Raman　microspectroscopy　can　provide　molecular-level　finger-print　information　about　the　biochemical　composition　and　structure　of　cells　and　tissues　with　excellent　spatial　resolution.　Raman　spectroscopy　of　nasopharyngeal　carcinoma　cell　lines　C666-1,　CNE2　and　normal　nasopharyngeal　cell　line　NP69　are　presented　to　investigate　the　differences　between　them.　The　ratio　of　I1449/I1657(1.10)　seems　to　very　easily　divide　tumor　and　normal　cell　lines　into　two　groups,　and　this　result　is　coincident　with　the　existing　report　about　the　study　of　normal　and　malignant　bronchial　tissue.　Principal　component　analysis　(PCA)　and　linear　discriminant　analysis　(LDA)　are　also　used　to　classify　different　cell　lines　and　achieve　an　exciting　result　with　a　sensitivity　and　specificity　of　90%　and　100%,　respectively.　The　results　of　the　work　may　be　helpful　to　the　discrimination　of　normal　and　tumor　cells,　which　show　that　Raman　spectroscopy　can　be　one　of　the　diagnostic　methods　of　nasopharyngeal　carcinoma　and　do　favor　to　the　early　diagnosis.