Raman　spectroscopy　can　provide　molecular-level　fingerprint　information　about　the　biochemical　composition　and　structure　of　cells　and　tissues　with　excellent　spatial　resolution.　In　this　study,　Raman　spectroscopy　of　3　different　nasopharyngeal　carcinoma　cell　lines　C666-1,　CNE1,　and　CNE2　and　1　nasopharyngeal　normal　cell　line　NP69　acquired　on　a　piece　of　silica　glass　slide　are　presented　to　investigate　the　differences　among　them.　The　results　show　the　ratio　of　I　1657/I1449　(=　0.7)　could　provide　good　distinction　between　tumor　and　normal　cell　lines　very　easily,　which　coincides　with　existing　reports　about　the　study　of　different　cell　lines　and　bronchial　tissue.　In　addition,　several　statistical　analytical　methods　were　used　to　classify　these　4　different　cell　lines　and　then　achieved　an　exciting　result　with　great　sensitivity　and　specificity　of　＞90%,　respectively.　The　findings　of　this　work　further　support　former　work　where　cells＇　Raman　spectra　were　acquired　on　a　different　substrate.　All　of　these　results　indicate　Raman　spectroscopy　has　the　potential　to　discriminate　between　normal　and　tumor　cells　and　have　potential　use　in　early　diagnosis　of　nasopharyngeal　carcinoma.　©　2013　Published　by　NRC　Research　Press.