Collagen　mimetic　peptides　(CMPs)　incorporating　the　triple-helical　sequence　or/and　integrin-binding　sequence　were　designed.　To　evaluate　biocompatibility　of　CMPs　in　vitro,　fibroblast　cells　(L929)　were　cultured.　The　proliferation　and　attachment　of　cell　were　observed　in　certain　time.　It　is　demonstrated　that　all　CMPs　had　no　obvious　effect　on　the　proliferation　of　L929　cells.　The　results　also　show　that　all　CMPs　promote　migration　and　attachment　of　L929　to　the　coated　surface.　And　the　cells　attachment　shape　and　proliferation　rate　of　L929　were　good.　After　coating　with　CMP27　which　containing　the　triple-helical　sequence　and　integrin-binding　sequence,　the　cell　adhesion　and　spreading　of　L929　were　significantly　improved　compared　with　others,　and　comparable　to　that　observed　on　type　I　collagen.　The　triple-helical　sequence　and　the　integrin-binding　sequence　were　shown　to　be　important　roles　on　promoting　cell　adhesion　collaboratively.　Therefore,　CMP　is　effective　for　improving　the　bioactivity　of　cell　adhesion,　and　could　be　potentially　used　as　an　adhesive　for　biomedical　application.　Moreover,　this　study　provided　new　insights　in　designing　other　peptide-based　bioactivity　materials.