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[期刊论文]

A signal-on fluorescence biosensor for detection of adenosine triphosphate based on click chemistry

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author:

Sun, Ning (Sun, Ning.) [1] | Guo, Qi (Guo, Qi.) [2] | Shao, Jingwei (Shao, Jingwei.) [3] | Unfold

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EI

Abstract:

A novel signal-on fluorescence biosensor for detection of adenosine triphosphate (ATP) based on the target induced recombination of split aptamer fragments and click chemistry is proposed. The CuS NPs modified on the streptavidin magnesphere paramagnetic particles (PMPs) can be destroyed by acid to form Cu(ii). In the presence of sodium ascorbate, Cu(ii) can be reduced to Cu(i) which in turn catalyzes the reaction between a weak-fluorescent 3-azido-7-hydroxycoumarin and propargyl alcohol to form strongly fluorescent 1,2,3-triazole compounds. The proposed sensor has been applied to the detection of ATP in human serum samples with satisfactory results. © the Partner Organisations 2014.

Keyword:

Adenosinetriphosphate Biosensors Copper compounds Fluorescence Phosphates Proteins Signal detection Synthesis (chemical)

Community:

  • [ 1 ] [Sun, Ning]Department of Applied Biology and Chemical Technology, Hong Kong Polytechnic University, Hunghom, Kowloon, Hong Kong
  • [ 2 ] [Guo, Qi]Department of Pharmacy, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China
  • [ 3 ] [Shao, Jingwei]Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350002, China
  • [ 4 ] [Qiu, Bin]Department of Applied Biology and Chemical Technology, Hong Kong Polytechnic University, Hunghom, Kowloon, Hong Kong
  • [ 5 ] [Qiu, Bin]Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350002, China
  • [ 6 ] [Lin, Zhenyu]Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350002, China
  • [ 7 ] [Wong, K.Y.]Department of Applied Biology and Chemical Technology, Hong Kong Polytechnic University, Hunghom, Kowloon, Hong Kong
  • [ 8 ] [Chen, Guonan]Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350002, China

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Source :

Analytical Methods

ISSN: 1759-9660

Year: 2014

Issue: 10

Volume: 6

Page: 3370-3374

1 . 8 2 1

JCR@2014

2 . 7 0 0

JCR@2023

ESI HC Threshold:268

JCR Journal Grade:2

CAS Journal Grade:3

Cited Count:

WoS CC Cited Count:

SCOPUS Cited Count: 12

30 Days PV: 2

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