A　novel　signal-on　fluorescence　biosensor　for　detection　of　adenosine　triphosphate　(ATP)　based　on　the　target　induced　recombination　of　split　aptamer　fragments　and　click　chemistry　is　proposed.　The　CuS　NPs　modified　on　the　streptavidin　magnesphere　paramagnetic　particles　(PMPs)　can　be　destroyed　by　acid　to　form　Cu(ii).　In　the　presence　of　sodium　ascorbate,　Cu(ii)　can　be　reduced　to　Cu(i)　which　in　turn　catalyzes　the　reaction　between　a　weak-fluorescent　3-azido-7-hydroxycoumarin　and　propargyl　alcohol　to　form　strongly　fluorescent　1,2,3-triazole　compounds.　The　proposed　sensor　has　been　applied　to　the　detection　of　ATP　in　human　serum　samples　with　satisfactory　results.　©　the　Partner　Organisations　2014.