In　this　research,　a　recombinant　Pichia　pastoris　strain　GS-BBA　overexpressing　barley　β-amylase　was　developed　using　the　recombinant　expression　plasmid　pPIC-BBA.　Strain　GS-BBA　could　produce　approximately　180　U/mL　β-amylase　in　shaking　flask　culture.　The　biochemical　properties　of　the　recombinant　β-amylase　were　examined.　The　maximum　activity　was　achieved　under　the　conditions　of　pH　5.0　and　55.　Meanwhile,　its　excellent　thermostability　and　pH　stability　was　observed　at　a　temperature　not　exceeding　55　and　in　the　pH　range　of　3.0-10.0.　The　recombinant　enzyme　could　hydrolyze　starch　to　form　maltose　as　the　major　product.　With　the　aid　of　pullulanase,　it　hydrolyzed　starch　to　form　maltose　with　a　conversion　efficiency　up　to　78.28%.　©　2016,　Editorial　Office　of　Food　Science.　All　right　reserved.