Aim:　This　study　aimed　to　develop　an　enzyme-linked　immunosorbent　assay　(ELISA)　method　for　detecting　the　residues　of　furazolidone　(FZD)　in　animal　food.　Method:　The　indirect　competitive　ELISA　method　based　on　single　chain　fragment　antibody　was　established.　Result:　The　optimal　antigen　mass　concentration　was　2　μg/mL,　and　the　optimal　antibody　dilution　ratio　was　1:500,　and　the　optimal　reaction　time　of　primary　antibody,　the　optimal　reaction　time　of　secondary　antibody　and　the　optimal　reaction　time　of　TMB　were　60　min,　45　min,　and　20　min,　respectively.　The　good　linearity　was　seen　in　the　range　of　10-100　ng/mL　of　FZD,　with　the　IC50　value　being　13.01　ng/mL,　and　the　lowest　detection　limit　(LOD)　being　1.28　ng/mL,　and　the　recovery　rates　being　73.38%-84.52%.　Conclusion:　Compared　with　the　monoclonal　antibody　against　FZD,　the　detection　kit　based　on　single　chain　fragment　antibody　displayed　wider　detection　range,　higher　sensitivity,　better　specificity　and　detection　stability.　©　2017,　China　Food　Publishing　Company.　All　right　reserved.