Lysozyme　with　a　small　monomeric　globular　enzymatic　protein　is　part　of　the　innate　immune　system,　and　its　deficiency　can　cause　the　increased　incidence　of　disease.　Herein,　we　devise　a　new　signal-enhanced　fluorescence　aptasensing　platform　for　quantitative　screening　of　lysozyme　by　coupling　with　rolling　circle　amplification　(RCA)　and　strand　hybridization　reaction,　accompanying　the　assembly　of　CdTe/CdSe　quantum　dots　(QDs)　and　hemin/G-quadruplex　DNzyme.　Initially,　target–triggered　release　of　the　primer　was　carried　out　from　DNA　duplex　via　the　reaction　of　the　aptamer　with　the　analyte,　and　the　released　primer　could　be　then　utilized　as　the　template　to　produce　numerous　repeated　oligonucleotide　sequences　by　the　RCA　reaction.　Following　that,　the　formed　long-stranded　DNA　simultaneously　hybridized　with　the　CdTe/CdSe　QD-labeled　probe　and　hemin/G-quadruplex　DNzyme　strand　in　the　system,　thereby　resulting　in　the　quenching　of　QD　fluorescent　signal　through　the　proximity　hemin/G-quadruplex　DNzyme　on　the　basis　of　transferring　photoexcited　conduction　band　electrons　of　quantum　dots　to　Fe(III)/Fe(II)-protoporphyrin　IX　(hemin)　complex.　Under　optimal　conditions,　the　fluorescent　signal　decreased　with　the　increasing　target　lysozyme　within　the　dynamic　range　from　5.0　to　500 nM　with　a　detection　limit　(LOD)　of　2.6 nM　at　the　3sblank　criterion.　Intra-assay　and　interassay　coefficients　of　variation　(CVs)　were　below　8.5%　and　11.5%,　respectively.　Finally,　the　system　was　applied　to　analyze　spiked　human　serum　samples,　and　the　recoveries　in　all　cases　were　85–111.9%.　©　2016　Elsevier　B.V.