The　amino-functionalized　metal　organic　framework　(NH2-Cu-MOF)　was　fabricated　via　bottom-up　synthesis　strategy.　Detailed　characterization　using　electron　microscopy,　X-ray　photoelectron　spectroscopy　and　atomic　force　microscopy　demonstrated　that　the　as-synthesized　NH2-Cu-MOF　was　two-dimensional　nanosheet　(a　thin　thickness　∼4.2　nm).　Results　showed　that　the　as-synthesized　NH2-Cu-MOF　nanosheet　possessed　fluorescence　property　(λem　=　425　nm)　as　well　as　peroxidase　mimic　activity.　On　the　basis　of　these　two　properties,　we　proposed　a　biosensor　for　hypoxanthine　detection.　The　fluorescence　intensity　had　a　linear　relationship　with　the　hypoxanthine　concentration　in　the　range　of　10–2000　μM.　The　limit　of　detection　was　3.93　μM　(S/N　=　3).　This　work　contributes　to　the　synthesis　of　a　new　two-dimensional　MOF　nanosheet　and　extends　the　application　of　two-dimensional　MOF　nanosheet　on　biological　luminescent　sensors.　©　2018