This　study　isolated　and　identified　the　lutein-enriching　microalga　Chlorella　sorokiniana　FZU60.　Different　types　of　media　and　concentrations　of　sodium　acetate　and　nitrate　were　evaluated　to　improve　mixotrophic　growth　and　lutein　production.　Highest　lutein　content,　production,　and　productivity　were　obtained　in　BG11　medium　with　1　g/L　acetate　and　0.75　g/L　nitrate.　Additionally,　pulse　feeding　with　1　g/L　acetate　every　48　h　led　to　the　alternation　between　mixotrophy　and　photoinduction,　resulting　in　a　lutein　production　of　33.6　mg/L.　Most　notably,　excellent　lutein　content　(9.57　mg/g)　and　productivity　(11.57　mg/L/d)　were　obtained　using　a　new　multi-operation　integrated　strategy,　and　the　achieved　levels　exceed　those　reported　in　most　related　studies.　This　work　demonstrates　the　synergistic　integration　of　simple　and　effective　strategies　for　the　enhancement　of　lutein　production　in　the　indigenous　microalga　C.　sorokiniana　FZU60　and　provides　new　insight　into　the　highly　efficient　microalgae-based　lutein　production.　©　2019　Elsevier　Ltd