Despite　aminoglycosides　(AGs)　have　superior　antibacterial　ability,　all　approved　AGs　by　FDA　are　associated　with　adverse　effects　such　as　ototoxicity　and　nephrotoxicity.　To　solve　these　problems,　AGs　hydrogels　based　on　dynamic　covalent　bond　cross-linking　were　quickly　prepared　within　25　s　by　using　AGs,　aldehyde　hyaluronic　acid　(A-HA),　and　adipic　acid　dihydrazide　graft　hyaluronic　acid　(HA-ADH)　as　materials.　FT-IR,　thermal　analysis,　and　SEM　results　exhibited　that　A-HA/HA-ADH/AGs　hydrogels　were　successfully　synthesized　with　highly　porous　and　interconnected　network　structure.　The　water　absorption　ratio　of　the　hydrogels　increased　with　the　decreasing　pH　and　temperature,　indicating　the　hydrogels　were　pH-　and　temp-responsive.　The　pH-dependent　degradation　also　demonstrated　pH　sensitivity　of　the　hydrogels.　Rheology　and　self-healing　analysis　assessment　displayed　that　AGs　hydrogels　had　good　mechanical　property,　self-healing　ability,　and　injectability.　The　hydrogels　had　no　cytotoxicity　to　L929　cells　and　their　hemolysis　ratios　were　between　0.7%　and　1.3%,　which　reached　a　nontoxic　level.　Most　importantly,　inhibition　zones　results　demonstrated　that　the　hydrogels　had　excellent　and　sustained　antibacterial　performance　against　Escherichia　coli　and　Staphylococcus　aureus.　Therefore,　A-HA/HA-ADH/AGs　hydrogels　are　potential　dressings　for　wound　healing.　Further　plans　including　antibacterial　and　in　vivo　wound　healing　assays　will　be　shown　in　the　next　work.　©　2020　Wiley　Periodicals,　Inc.