A　new　chemiluminescent　probe　10,　10＇-dimethyl-3,3＇-disulfo-9,9＇-biacridine　(DMDSBA)　is　used　to　label　anti-CEA　antibody,　the　molar　incorporation　is　1.15-1.32　mole　of　DMDSBA　per　mol　anti-CEA;　the　conditions　of　labeling　reaction,sandwich　immunoassay　and　chemiluminescent　reaction　are　studied.　Morever,　a　new　double　antibody　sandwich　chemiluminescence　immunoassay　is　firstly　established,the　linear　calibration　ranges　is　1.5-150.0　ng.mL(-1)　and　the　detection　limit　is　0.53　ng.mL(-1).　This　method　is　used　to　detect　cancer　embryo　antigen　(CEA)　in　human　serum,the　results　are　well-matched　with　that　of　radio　immunoassay.