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author:

Guo, LQ (Guo, LQ.) [1] | Xie, ZH (Xie, ZH.) [2] (Scholars:谢增鸿) | Lin, XC (Lin, XC.) [3] (Scholars:林旭聪) | Ke, ZH (Ke, ZH.) [4] | Chen, GN (Chen, GN.) [5] (Scholars:陈国南)

Indexed by:

SCIE PKU CSCD

Abstract:

Direct fluorophotometric and flow-injection fluorophotometric methods for trace ammonia measurement involving the fluorescent reaction of ammonia with o-phthaldlaldehyde (OPA) and 2-mercaptoethanol(ME) producing derivant of iso-indol in alkaline solution are described. Triton X-100 was added to improve the stability of the system. The maximum excitation wavelength is 415 nm, while the maximum emission wavelength is 486 nm. With direct fliuorophotometric method, the calibration curve's regression equation is DeltaI = 22.286 + 785.71c(NH3) (mug(.)ml(-1)) (r = 0.999 5) in the range of ammonia concentration from 0.2 to 1.0 mug(.)mL(-1), and DeltaI = -27.429 + 2 371.4c(NH3) (mug(.)ml(-1)) (r = 0.996 5) in the range of ammonia concentration from 0.02 to 0.10 mug(.)mL(-1). With flow-injection fluorophotometric method, the calibration curve's regression equation is DeltaI = 1188c(NH3) (mug(.)mL(-1))(r = 0.999 8) in the range of ammonia concentration of ammonia 0(.)0.7 mug(.)mL(-1). The mechanism of reaction was also briefly discussed.

Keyword:

ammonia flow-injection analysis fluorophotometry nonionic surfactant

Community:

  • [ 1 ] Fuzhou Univ, Dept Chem, Fuzhou 350002, Peoples R China

Reprint 's Address:

  • 郭良洽

    [Guo, LQ]Fuzhou Univ, Dept Chem, Fuzhou 350002, Peoples R China

Email:

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Source :

SPECTROSCOPY AND SPECTRAL ANALYSIS

ISSN: 1000-0593

CN: 11-2200/O4

Year: 2004

Issue: 7

Volume: 24

Page: 851-854

0 . 3 5

JCR@2004

0 . 7 0 0

JCR@2023

ESI Discipline: CHEMISTRY;

JCR Journal Grade:4

Cited Count:

WoS CC Cited Count:

SCOPUS Cited Count:

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count:

30 Days PV: 2

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