A　novel　lysozyme　exhibiting　antifungal　activity　and　with　a　molecular　mass　of　14.4　kDa　in　SDS-polyacrylamide　gel　electrophoresis　was　isolated　from　mung　bean　(Phaseolus　mungo)　seeds　using　a　procedure　that　involved　aqueous　extraction,　ammonium　sulfate　precipitation,　ion　exchange　chromatography　on　CM-Sephadex,　and　high-performance　liquid　chromatography　on　POROS　HS-20.　Its　N-terminal　sequence　was　very　different　from　that　of　hen　egg　white　lysozyme.　Its　pI　was　estimated　to　be　above　9.7.　The　specific　activity　of　the　lysozyme　was　355　U/mg　at　pH　5.5　and　30　degreesC.　The　lysozyme　exhibited　a　pH　optimum　at　pH　5.5　and　a　temperature　optimum　at　55　degreesC.　It　is　reported　herein,　for　the　first　time,　that　a　novel　plant　lysozyme　exerted　an　antifungal　action　toward　Fusarium　oxysporum,　Fusarium　solani,　Pythium　aphanidermatum,　Sclerotium　rolfsii,　and　Botrytis　cinerea,　in　addition　to　an　antibacterial　action　against　Staphylococcus　aureus.　(C)　2004　Elsevier　Inc.　All　rights　reserved.