A　novel　lysozyme　exhibiting　antifungal　activity　and　with　a　molecular　mass　of　14.4　kDa　in　SDS-polyacrylamide　gel　electrophoresis　was　isolated　from　mung　bean　(Phaseolus　mungo)　seeds　using　a　procedure　that　involved　aqueous　extraction,　ammonium　sulfate　precipitation,　ion　exchange　chromatography　on　CM-Sephadex,　and　high-performance　liquid　chromatography　on　POROS　HS-20.　Its　N-terminal　sequence　was　very　different　from　that　of　hen　egg　white　lysozyme.　Its　pI　was　estimated　to　be　above　9.7.　The　specific　activity　of　the　lysozyme　was　355　U/mg　at　pH　5.5　and　30°C.　The　lysozyme　exhibited　a　pH　optimum　at　pH　5.5　and　a　temperature　optimum　at　55°C.　It　is　reported　herein,　for　the　first　time,　that　a　novel　plant　lysozyme　exerted　an　antifungal　action　toward　Fusarium　oxysporum,　Fusarium　solani,　Pythium　aphanidermatum,　Sclerotium　rolfsii,　and　Botrytis　cinerea,　in　addition　to　an　antibacterial　action　against　Staphylococcus　aureus.　©　2004　Elsevier　Inc.　All　rights　reserved.