Transformation　of　vesicles　formed　in　DTAB/C-11-p-FhCNa　aqueous　surfactant　two-phase　(ASTP)　was　observed　by　the　transmission　electron　microscopy　(TEM).　The　trans-conformation　of　the　gemini　surfactant　in　the　aggregates　was　considered　to　be　the　important　factor　for　constructing　the　multi-lamellar　structure　of　the　vesicle　wall.　The　cation-pi　interaction　between　the　quaternary　ammonium　cation　and　the　aromatic　ring　in　the　spacer　was　determined　by　the　UV-Vis　spectrum　analysis,　which,　as　well　as　the　general　electrostatic　attraction　and　hydrophobic　force,　contributes　to　the　stability　of　the　multi-lamellar　structure.　The　concentrations　of　the　surface-active　ions　were　measured　for　understanding　the　mechanism　of　vesicle　transformation.　The　results　show　that　isoelectric　mixing　of　the　two　components　benefits　the　growth　of　vesicles　both　in　size　and　wall　thickness.