A　new　acridone　derivative　2-nitroacridone　(NAD)　was　synthesized　in　this　paper,　and　it　was　found　that　NAD　had　excellent　electrochemical　activity　on　the　glassy　carbon　electrode　(GCE)　with　a　couple　reversible　redox　peaks　at　0.051　V　and　0.103　V,　respectively.　Voltammetry　was　used　to　investigate　the　electrochemical　behavior　of　NAD　and　the　interaction　between　NAD　and　salmon　sperm　DNA.　In　pH　4.0　phosphate　buffer　solution,　the　binding　ratio　between　NAD　and　salmon　sperm　DNA　was　calculated　to　be　2:1　and　the　binding　constant　was　3.19　x　10(5)　L/mol.　A　Chronic　Myelogenous　Leukemia　(CML,　Type　b(3)a(2))　DNA　biosensor　was　developed　by　immobilizing　covalently　single-stranded　CML　DNA　fragments　to　a　modified　GCE.　The　surface　hybridization　of　the　immobilized　single-stranded　CML　DNA　fragment　with　its　complementary　DNA　fragment　was　evidenced　by　electrochemical　methods　using　NAD　as　a　novel　electrochemical　indicator,　with　a　detection　limit　of　6.7　x　10(-9)　M　and　a　linear　response　range　of　1.8　x　10(-8)　M　to　9.1　x　10(-8)　M　for　CML　DNA.　Selective　determination　of　complementary　ssDNA　was　achieved　using　differential　pulse　voltammetry　(DPV).　(C)　2008　Elsevier　B.V.　All　rights　reserved.