A　fabrication　strategy　for　developing　new　electrochemical　interface　based　on　graphite/poly(methylmethacrylate)　(PMMA)　composite　electrode　for　some　biological　molecules　was　proposed.　The　results　indicated　that　this　electrochemical　sensing　interface　has　the　advantages,　such　as　quick　electron-transfer　rate,　avoidance　or　electrode　fouling,　highly　sensitive　and　stable　amperometric　sensing　for　some　biomolecules.　Compared　with　carbon　paste　electrode,　this　interface　would　effectively　minimize　the　over-potential　and　enhance　the　electrochemical　response　to　some　biomolecules.　In　order　to　further　evaluate　the　high　performance　of　this　interface,　the　electrochemical　behaviors　of　NADH　at　this　sensing　interface　were　investigated　in　detail.　(C)　2008　Elsevier　B.V.　All　rights　reserved.