A　novel,　simple　and　sensitive　method　for　the　enantioseparation　and　determination　of　DL-tetrahydropalmatine　(DL-THP)　was　developed　using　ACE　in　combination　with　partial　filling　technique　and　field-amplified　sample　injection.　A　chiral　selector,　i.e.　BSA,　was　used　for　the　enantioseparation　of　DL-THP　in　ACE.　Effects　of　BSA　concentration,　pH　and　separation　voltage　on　the　effectiveness　of　the　enantiomer　separation　were　evaluated.　In　an　optimal　condition,　D-　and　L-THP　were　completely　enantio-separated　in　less　than　9　min　by　partially　filling　an　electrophoretic　capillary　with　50　mu　mol/L　BSA　(50　mbar,　100　s)　and　carrying　out　an　electrophoresis　with　20　mmol/L　phosphate　buffer　(pH　7.4)　at　15　kV.　The　sensitivity　was　further　improved　by　making　use　of　field-amplified　sample　injection　to　lower　the　LOD　(defined　as　S/N　=　3)　down　to　6　ng/mL.　Real　samples　were　also　tested　and　promising　results　for　the　determination　of　DL-THP　enantiomers　were　obtained.