The　rebinding　behavior　of　protein-imprinted　hydrogel　is　different　from　classical　small　molecularly　imprinted　polymer　especially　in　protein　aggregating　state　and　interaction　changeability　between　protein　and　imprinted　sites.　BSA-imprinted　calcium　alginate-hydroxy　ethyl　cellulose　microspheres　were　prepared,　and　the　rebinding　behavior　was　studied.　An　＂imprinting　induce-fit　model＇＇　was　proposed　to　describe　the　rebinding　property　of　protein-imprinted　hydrogel.　Three　kinds　of　different　interacting　forms　between　protein　and　imprinted　hydrogel　were　discovered　by　Scatchard　analysis.　Slogistic　fit　analysis　of　rebinding　rate　coefficient　was　carried　out,　and　the　imprinted　hydrogel　was　found　capable　of　promoting　rebinding　through　induce-fit　behavior.　Higher　imprinting　efficiency　was　found　in　microsphere　samples　with　lower　crosslinking　density.　Rebinding　regression　equation　was　established,　and　the　rebinding　quantity　was　computable　with　parameters　including　BSA　aggregate　concentration　[P(n)],　dissociating　rate　(k(1)),　and　single　molecule　rebinding　rate　(k(2)).　The　experimental　and　calculated　rebinding　concentration　were　compared,　and　errors　between　-9.43%　and　5.59%　were　found.　(C)　2011　Wiley　Periodicals,　Inc.　J　Appl　Polym　Sci　122:　1847-1856,　2011