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Abstract:
A novel device was designed for the multipoint addressable detection of DNA hybridization. Row and column electrodes array were orthogonally arranged, and the microwells were assembled on the crossing points of the row/column electrodes to form a 4 x 4 microwell array. Amperometric signals at the individual microwells could be detected separately on the basis of redox cycling of localized electroactive species occurring between the electrodes. Immobilization and hybridization of DNA could block the redox cycling of Fe(CN)(6)(4-)/Fe(CN)(6)(3-) at the designated microwells, resulting in the reduction of current response. This device had been used to detect DNA hybridization with excellent sensitivity (0.03 mu M) and selectivity. The device can be applied to comprehensive and high-throughput detection and imaging of biochemical species. Crown Copyright (C) 2011 Published by Elsevier B.V. All rights reserved.
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SENSORS AND ACTUATORS B-CHEMICAL
ISSN: 0925-4005
Year: 2011
Issue: 1
Volume: 160
Page: 923-928
3 . 8 9 8
JCR@2011
8 . 0 0 0
JCR@2023
ESI Discipline: CHEMISTRY;
JCR Journal Grade:1
CAS Journal Grade:1
Cited Count:
SCOPUS Cited Count: 19
ESI Highly Cited Papers on the List: 0 Unfold All
WanFang Cited Count:
Chinese Cited Count:
30 Days PV: 1
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