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author:

Shah, Jaivik V. (Shah, Jaivik V..) [1] | Patel, Daxesh P. (Patel, Daxesh P..) [2] | Shah, Priyanka A. (Shah, Priyanka A..) [3] | Sanyal, Mallika (Sanyal, Mallika.) [4] | Shrivastav, Pranav S. (Shrivastav, Pranav S..) [5]

Indexed by:

Scopus SCIE

Abstract:

A simple, sensitive and reproducible ultra-performance liquid chromatography-tandem mass spectrometry method has been developed for the simultaneous determination of atenolol, a -adrenergic receptor-blocker and chlorthalidone, a monosulfonamyl diuretic in human plasma, using atenolol-d7 and chlorthalidone-d4 as the internal standards (ISs). Following solid-phase extraction on Phenomenex Strata-X cartridges using 100L human plasma sample, the analytes and ISs were separated on an Acquity UPLC BEH C-18 (50mmx2.1mm, 1.7 mu m) column using a mobile phase consisting of 0.1% formic acid-acetonitrile (25:75, v/v). A tandem mass spectrometer equipped with electrospray ionization was used as a detector in the positive ionization mode for both analytes. The linear concentration range was established as 0.50-500ng/mL for atenolol and 0.25-150ng/mL for chlorthalidone. Extraction recoveries were within 95-103% and ion suppression/enhancement, expressed as IS-normalized matrix factors, ranged from 0.95 to 1.06 for both the analytes. Intra-batch and inter-batch precision (CV) and accuracy values were 2.37-5.91 and 96.1-103.2%, respectively. Stability of analytes in plasma was evaluated under different conditions, such as bench-top, freeze-thaw, dry and wet extract and long-term. The developed method was superior to the existing methods for the simultaneous determination of atenolol and chlorthalidone in human plasma with respect to the sensitivity, chromatographic analysis time and plasma volume for processing. Further, it was successfully applied to support a bioequivalence study of 50mg atenolol+12.5mg chlorthalidone in 28 healthy Indian subjects. Copyright (c) 2015 John Wiley & Sons, Ltd.

Keyword:

atenolol bioequivalence chlorthalidone human plasma sensitive ultra-performance liquid chromatography-tandem mass spectrometry

Community:

  • [ 1 ] [Shah, Jaivik V.]Gujarat Univ, Sch Sci, Dept Chem, Ahmadabad 380009, Gujarat, India
  • [ 2 ] [Shah, Priyanka A.]Gujarat Univ, Sch Sci, Dept Chem, Ahmadabad 380009, Gujarat, India
  • [ 3 ] [Shrivastav, Pranav S.]Gujarat Univ, Sch Sci, Dept Chem, Ahmadabad 380009, Gujarat, India
  • [ 4 ] [Patel, Daxesh P.]Fuzhou Univ, Canc Metastasis Alert & Prevent Ctr, Fujian 350002, Peoples R China
  • [ 5 ] [Sanyal, Mallika]St Xaviers Coll, Dept Chem, Ahmadabad 380009, Gujarat, India

Reprint 's Address:

  • [Shrivastav, Pranav S.]Gujarat Univ, Sch Sci, Dept Chem, Ahmadabad 380009, Gujarat, India

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Source :

BIOMEDICAL CHROMATOGRAPHY

ISSN: 0269-3879

Year: 2016

Issue: 2

Volume: 30

Page: 208-216

1 . 6 1 3

JCR@2016

1 . 8 0 0

JCR@2023

ESI Discipline: CHEMISTRY;

ESI HC Threshold:235

JCR Journal Grade:3

CAS Journal Grade:4

Cited Count:

WoS CC Cited Count: 16

SCOPUS Cited Count: 16

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count:

30 Days PV: 2

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