Environmental　endocrine　disruptors　such　as　17　beta-estradiol,　are　widely　distributed　with　low　concentration　in　the　water,　which　has　great　harm　to　ecosystems　and　human　health.　To　improve　the　detection　sensitivity　of　17　beta-estradiol,　a　Ru　(bpy)　(2+)(3)/MWCNTs-Nafion-SiO2　modified　electrode　was　firstly　made　by　electrostatic　adsorption　of　multi-walled　carbon　nanotubes　(MWCNTs)　and　ion　exchange　of　Nafion,　binding　nano.　silica　and　immobilizing　Ru(bpy)　(2+)(3)　on　the　surface　of　gold　electrode.　And　then　a　molecular　imprinting-electrochemiluminescence　sensor　(ECL-MIPs)　was　acquired　by　modifying　the　molecular　imprinted　membrane　with　sol-gel　method　for　improving　the　specific　selectivity.　Under　the　optimum　conditions　(in　0.　1　mol/L　PBS,　pH　7.　4)　at　a　scan　rate　of　100　mV/s　and　accumulation　time　of　20　min),　there　was　a　good　linear　relationship　between　the　ECL　intensity　difference　and　17　beta-estradiol　concentration　in　the　range　of　0.　03-2　mu　g/L,　with　a　detection　limit　of　0.　006　mu　g/L.　The　ECL.　MIPs　sensor　was　successfully　applied　to　the　determination　of　17　beta-estradiol　in　the　water　samples　with　recoveries　from　88.　7%　to　105.　0%.