Hexagonal　boron　nitride　nanosheets　(BNNS),　one　kind　of　inorganic　graphene　analogue,　exhibit　high　fluorescence　quenching　ability　via　the　photo-induced　electron　transfer　(PET)　and　different　affinity　toward　single-stranded　DNA　(ssDNA)　and　double-stranded　DNA　(dsDNA).　As　a　proof　of　concept,　the　BNNS　was　first　used　as　a　sensing　platform　for　the　rapid　detection　of　DNA　and　small　molecules　with　high　sensitivity　and　selectivity　in　this　study.　This　strategy　is　versatile　and　quick　fluorescence　sensing　of　DNA　and　extensive　DNA　related　analytes　such　as　metal　cations　and　small　molecules.　Moreover,　this　strategy　might　be　available　for　the　practical　application　in　future.　This　work　provides　an　avenue　for　understanding　the　interaction　between　two-dimensional　nanomaterials　and　biomolecules　and　designing　novel　sensing　strategies　for　extending　the　applications　of　nanomaterials　in　bioanalysis.