In　this　article,　we　fabricated　a　bioactive　hydrogel　composed　of　glycol　chitosan　(G-CS)　and　oxidized　hyaluronic　acid　(OHA)　via　Schiff　base　reaction.　Cartilage　extracellular　matrix　(ECM)　particles　with　different　concentrations　were　used　to　functionalize　G-CS/OHA　(S1)　hydrogel.　The　results　demonstrated　that　S3　(G-CS/OHA/ECM　2%　w/v)　hydrogel　exhibited　the　most　suitable　compression　strength　and　provided　the　optimal　environment　for　proliferation　of　bone　marrow　mesenchymal　stem　cells　(BMSCs).　To　assess　the　chondroinductivity　of　ECM　in　vitro,　we　compared　the　chondrogenesis　of　BMSCs　in　S1　(G-CS/OHA)　and　S3　(G-CS/OHA/ECM　2%　w/v)　hydrogels.　The　results　confirmed　that　the　higher　levels　of　glycosaminoglycans　(GAGs)　and　collagen　type　II　(COL　II)　were　accumulated　in　S3　hydrogel.　In　vivo,　cartilage　defects　of　rats　generated　most　mature　tissue　within　BMSCs-laden　S3　hydrogel　(S3/BMSCs　group).　The　tissues　were　more　integrative　and　contained　higher　levels　of　COL　II　and　GAGs　compared　to　the　other　groups.　All　these　results　suggested　that　the　G-CS/OHA　hydrogel　functionalized　with　ECM　particles　is　a　good　candidate　biomaterial　to　be　applied　in　cartilage　tissue　engineering.