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author:

Zhang, Huifang (Zhang, Huifang.) [1] | Luo, Fang (Luo, Fang.) [2] (Scholars:罗芳) | Wang, Peilong (Wang, Peilong.) [3] | Guo, Longhua (Guo, Longhua.) [4] | Qiu, Bin (Qiu, Bin.) [5] (Scholars:邱彬) | Lin, Zhenyu (Lin, Zhenyu.) [6] (Scholars:林振宇)

Indexed by:

EI Scopus SCIE

Abstract:

A simple and sensitive electrochemiluminescence (ECL) aptasensor has been developed for bisphenol A (BPA) detection. The capture DNA (CDNA) was modified on the heated indium-tin-oxide (ITO) working electrode surface firstly and then hybridized with BPA aptamer to form double strand DNA (dsDNA). The presence of target can cause the releasing of aptamer from the electrode surface since the aptamer prefers to switch its configuration to combine with BPA. Subsequently, the free CDNA will induce hybridization chain reaction (HCR) to produce long dsDNA on the electrode surface. Ru(phen)(3)(2+) can integrate into the grooves of dsDNA to act as an ECL reagent, thus enhanced ECL signal can be detected. The temperature control during the processes of target recognition and HCR were realized through the heated electrode instead of the bulk solution heating. Furthermore, the performance of the ECL aptasensor can be further enhanced at elevated electrode temperature. Under the optimized conditions, the ECL intensity of the system has a linear relationship with the logarithm of BPA concentration in the range of 2.0 pM-50 nM. The limit of detection (LOD) at 55 degrees C (electrode surface temperature) was calculated to be 1.5 pM, which was approximately 6.5-fold lower than that at 25 degrees C. The proposed biosensor has been applied to detect the BPA in drink samples with satisfactory results.

Keyword:

Aptamer Bisphenol A Electrochemiluminescence Heated indium-tin-oxide electrode Hybridization chain reaction

Community:

  • [ 1 ] [Zhang, Huifang]Fuzhou Univ, Fujian Prov Key Lab Anal & Detect Technol Food Sa, MOE Key Lab Analyt Sci Food Safety & Biol, Inst Nanomed & Nanobiosensing,Coll Chem, Fuzhou 350116, Fujian, Peoples R China
  • [ 2 ] [Luo, Fang]Fuzhou Univ, Fujian Prov Key Lab Anal & Detect Technol Food Sa, MOE Key Lab Analyt Sci Food Safety & Biol, Inst Nanomed & Nanobiosensing,Coll Chem, Fuzhou 350116, Fujian, Peoples R China
  • [ 3 ] [Guo, Longhua]Fuzhou Univ, Fujian Prov Key Lab Anal & Detect Technol Food Sa, MOE Key Lab Analyt Sci Food Safety & Biol, Inst Nanomed & Nanobiosensing,Coll Chem, Fuzhou 350116, Fujian, Peoples R China
  • [ 4 ] [Qiu, Bin]Fuzhou Univ, Fujian Prov Key Lab Anal & Detect Technol Food Sa, MOE Key Lab Analyt Sci Food Safety & Biol, Inst Nanomed & Nanobiosensing,Coll Chem, Fuzhou 350116, Fujian, Peoples R China
  • [ 5 ] [Lin, Zhenyu]Fuzhou Univ, Fujian Prov Key Lab Anal & Detect Technol Food Sa, MOE Key Lab Analyt Sci Food Safety & Biol, Inst Nanomed & Nanobiosensing,Coll Chem, Fuzhou 350116, Fujian, Peoples R China
  • [ 6 ] [Zhang, Huifang]Gannan Normal Univ, Sch Chem & Chem Engn, Key Lab Organopharmaceut Chem Jiangxi Prov, Ganzhou 341000, Peoples R China
  • [ 7 ] [Wang, Peilong]China Agr Acad Sci, Inst Qual Stand & Testing Technol Agr Prod, Minist Agr, Key Lab Agrifood Safety & Qual, Beijing 100081, Peoples R China

Reprint 's Address:

  • 林振宇

    [Lin, Zhenyu]Fuzhou Univ, Fujian Prov Key Lab Anal & Detect Technol Food Sa, MOE Key Lab Analyt Sci Food Safety & Biol, Inst Nanomed & Nanobiosensing,Coll Chem, Fuzhou 350116, Fujian, Peoples R China;;[Wang, Peilong]China Agr Acad Sci, Inst Qual Stand & Testing Technol Agr Prod, Minist Agr, Key Lab Agrifood Safety & Qual, Beijing 100081, Peoples R China

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Source :

BIOSENSORS & BIOELECTRONICS

ISSN: 0956-5663

Year: 2019

Volume: 129

Page: 36-41

1 0 . 2 5 7

JCR@2019

1 0 . 7 0 0

JCR@2023

ESI Discipline: CHEMISTRY;

ESI HC Threshold:184

JCR Journal Grade:1

CAS Journal Grade:1

Cited Count:

WoS CC Cited Count: 44

SCOPUS Cited Count: 40

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count:

30 Days PV: 3

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