A　simple　and　sensitive　electrochemiluminescence　(ECL)　aptasensor　has　been　developed　for　bisphenol　A　(BPA)　detection.　The　capture　DNA　(CDNA)　was　modified　on　the　heated　indium-tin-oxide　(ITO)　working　electrode　surface　firstly　and　then　hybridized　with　BPA　aptamer　to　form　double　strand　DNA　(dsDNA).　The　presence　of　target　can　cause　the　releasing　of　aptamer　from　the　electrode　surface　since　the　aptamer　prefers　to　switch　its　configuration　to　combine　with　BPA.　Subsequently,　the　free　CDNA　will　induce　hybridization　chain　reaction　(HCR)　to　produce　long　dsDNA　on　the　electrode　surface.　Ru(phen)32+　can　integrate　into　the　grooves　of　dsDNA　to　act　as　an　ECL　reagent,　thus　enhanced　ECL　signal　can　be　detected.　The　temperature　control　during　the　processes　of　target　recognition　and　HCR　were　realized　through　the　heated　electrode　instead　of　the　bulk　solution　heating.　Furthermore,　the　performance　of　the　ECL　aptasensor　can　be　further　enhanced　at　elevated　electrode　temperature.　Under　the　optimized　conditions,　the　ECL　intensity　of　the　system　has　a　linear　relationship　with　the　logarithm　of　BPA　concentration　in　the　range　of　2.0　pM-50　nM.　The　limit　of　detection　(LOD)　at　55　°C　(electrode　surface　temperature)　was　calculated　to　be　1.5　pM,　which　was　approximately　6.5-fold　lower　than　that　at　25　°C.　The　proposed　biosensor　has　been　applied　to　detect　the　BPA　in　drink　samples　with　satisfactory　results.　©　2019　Elsevier　B.V.