Aldehyde　hyaluronic　acid-cisplatin　(A-HA-CDDP)　complex　nanoparticles　were　readily　prepared,　and　CDDP　was　stably　loaded　into　the　core　of　the　NPs　through　imine　bond　and　coordinate　bond　linkages.　The　results　show　that　the　NPs　were　prepared　successfully　by　a　chemical　complexation　reaction　rather　than　by　physical　mixing.　Compared　to　many　CDDP　and　HA　complex　nanoparticles　evaluated　in　other　studies,　A-HA-CDDP　NPs　with　imine　and　coordinate　bonds　between　the　A-HA　and　CDDP　displayed　better　sustained　release　behavior　and　pH　sensitivity.　Therefore,　the　acidic　tumor　environment　could　accelerate　the　release　of　CDDP　from　the　NPs.　MTT　and　AO/EB　staining　assays　showed　that　A-HA-CDDP　NPs　had　comparable　cell　inhibition　with　CDDP　in　HeLa　cells　as　well　as　little　toxicity　to　NIH3T3　cells.　This　result　indicates　that　the　chemical　reaction　between　A-HA　and　CDDP　had　little　effect　on　the　antitumor　activity　of　CDDP　and　that　the　NPs　actively　targeted　CD44-rich　tumor　cells.　Both　a　hemolysis　test　and　a　protein　adsorption　assay　demonstrated　that　A-HA-CDDP　NPs　had　good　biocompatibility　and　blood　circulation　in　vivo.　Therefore,　the　NPs　have　the　potential　to　be　used　for　targeted　CDDP　delivery　in　vivo.　A　subsequent　publication　will　describe　the　circulation,　targeting　and　tumor　inhibition　experiments　of　these　NPs　in　vivo.