Clinical　application　of　cisplatin　(CDDP)　against　various　solid　tumors　is　often　limited　due　to　its　poor　selectivity　and　severe　side　effect.　Considering　this,　in　our　study,　CDDP　was　incorporated　in　fluorescent　PEG　amine　grafted　aldehyde　hyaluronic　acid　by　imine　bond　and　metal　ion　coordination　bond　linking　and　formed　a　complex,　the　complex　was　then　self-assembled　into　nanoparticles　in　water　simply.　FT-IR,　XRD,　DLS　and　SEM　analysis　demonstrated　that　the　nanoparticles　were　prepared　successfully　and　exhibited　a　spherical　structure　with　size　ranged　from　216.4　to　372.3nm　in　diameter.　CDDP　releasing　from　the　nanoparticles　was　in　a　controlled　manner,　and　had　faster　release　rate　at　lower　pH,　indicating　the　nanoparticles　were　responsive　to　tumor　micro-acid　environment.　Since　fluorescent　Cy5.5　and　targeting　hyaluronic　acid　existed　on　the　surface　of　the　nanoparticles,　CLSM　images　clearly　showed　that　the　nanoparticles　could　target　and　internalize　into　HeLa　cells,　and　then　inhibited　the　growth　of　HeLa　cells.　In　addition,　MTT,　AO-EB　staining,　and　hemolysis　assay　showed　that　the　nanoparticles　had　good　cyto-/hemo-compatibility.　Hence,　the　nanoparticles　had　the　potential　to　be　used　for　cancer　therapy　and　diagnosis.　The　further　in　vivo　experiment　will　be　shown　in　the　next　work.　[GRAPHICS]　.