VeA　is　a　key　velvet　protein　that　regulates　sexual/asexual　development　and　secondary　metabolism　in　filamentous　fungi,　particularly　Aspergilli,　but　has　not　been　explored　yet　in　asexual　insect　mycopathogens,　such　as　Beauveria　bassiana.　Here,　we　report　a　localization　of　B.　bassiana　VeA　in　the　cytoplasm　of　hyphal　cells　exposed　to　either　light　or　dark　cue　and　its　migration　to　the　nucleus　only　in　darkness.　Deletion　of　veA　resulted　in　facilitated　hyphal　growth　and　decreased　cell　length　on　rich　media,　light　growth　defects　on　scant　media,　and　increased　sensitivities　to　oxidation,　high　osmolarity　and　prolonged　heat　shock　during　colony　growth.　Compared　to　wild-type,　the　deletion　mutant　was　much　more　triggered　in　conidiation　at　optimal　25　degrees　C　in　darkness　than　in　a　light/dark　(L:D)　cycle　of　12:12,　indicating　the　role　of　VeA　acting　as　a　negative　regulator　of　conidiation　in　a　light-dependent　manner.　The　mutant　conidia　produced　at　L:D　12:12　showed　defects　in　germination,　thermotolerance　and　UVB　resistance　but　no　change　in　virulence,　contrasting　to　attenuated　virulence　for　the　mutant　conidia　produced　in　darkness.　Intriguingly,　fungal　outgrowth　and　conidiation　were　markedly　suppressed　on　the　surfaces　of　the　mutant-mummified　insect　cadavers,　suggesting　a　significant　role　of　VeA　in　fungal　survival,　dispersal　and　prevalence　in　host　habitats.　Transcriptomic　analysis　revealed　1248　and　1183　differentially　expressed　genes　in　the　deletion　mutant　versus　wild-type　grown　at　L:D　0:24　and　12:12　respectively,　including　those　involved　in　central　developmental　pathway　and　secondary　metabolism.　Altogether,　VeA　is　functionally　involved　in　asexual　cycle,　stress　tolerance　and　transcriptional　regulation　of　B.　bassiana.