The　effectiveness　of　mesenchymal　stem　cells　(MSC)　in　the　treatment　of　cartilage　diseases　has　been　demonstrated　to　be　attributed　to　the　paracrine　mechanisms,　especially　the　mediation　of　exosomes.　But　the　exosomes　derived　from　unsynchronized　MSCs　may　be　nonhomogeneous　and　the　therapeutic　effect　varies　between　samples.　Aim:　To　produce　homogeneous　and　more　effective　exosomes　for　the　regeneration　of　cartilage.　Materials　&　methods:　In　this　study　we　produced　specific　exosomes　from　bone　marrow　MSCs　(BMSC)　through　kartogenin　(KGN)　preconditioning　and　investigated　their　performance　in　either　in　vitro　or　in　vivo　experiments.　Results　&　conclusion:　The　exosomes　derived　from　KGN-preconditioned　BMSCs　(KGN-BMSC-Exos)　performed　more　effectively　than　the　exosomes　derived　from　BMSCs　(BMSC-Exos).　KGN　preconditioning　endowed　BMSC-Exos　with　stronger　chondral　matrix　formation　and　less　degradation.