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学者姓名：陈岚岚
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Abstract ：
Labile heme (LH) is an important signaling molecule in virtually all organisms. However, specifically detecting LH remains an outstanding challenge. Herein, by learning from the bioactivation mechanism of artemisinin, we have developed the first LH-responsive small-molecule fluorescent probe, HNG, based on a 4-amino-1,8-naphthalimide (NG) fluorophore. HNG showed high selectivity for LH without interference from hemin, protein-interacting heme, and zinc protoporphyrin. Using HNG, the changes of LH levels in live cells were imaged, and a positive correlation of LH level with the degree of hemolysis was uncovered in hemolytic mice. Our study not only presents the first molecular probe for specific LH detection but also provides a strategy to construct probes with high specificity through a bioinspired approach.
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| GB/T 7714 | Xu, Shuai , Liu, Hong-Wen , Chen, Lanlan et al. Learning from Artemisinin: Bioinspired Design of a Reaction-Based Fluorescent Probe for the Selective Sensing of Labile Heme in Complex Biosystems [J]. | JOURNAL OF THE AMERICAN CHEMICAL SOCIETY , 2020 , 142 (5) : 2129-2133 . |
| MLA | Xu, Shuai et al. "Learning from Artemisinin: Bioinspired Design of a Reaction-Based Fluorescent Probe for the Selective Sensing of Labile Heme in Complex Biosystems" . | JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 142 . 5 (2020) : 2129-2133 . |
| APA | Xu, Shuai , Liu, Hong-Wen , Chen, Lanlan , Yuan, Jie , Liu, Yongchao , Teng, Lili et al. Learning from Artemisinin: Bioinspired Design of a Reaction-Based Fluorescent Probe for the Selective Sensing of Labile Heme in Complex Biosystems . | JOURNAL OF THE AMERICAN CHEMICAL SOCIETY , 2020 , 142 (5) , 2129-2133 . |
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Abstract ：
Elevated levels of intracellular hydrogen peroxide (H2O2) are closely related to the development of cancers. Specific imaging of H2O2 in tumor sites would be significant not only for cancer diagnosis but also for gaining a deep understanding of the role of H2O2 in cancer. However, traditional fluorescent probes based only on responses to overexpression levels of H2O2 in cancer cells are insufficient to distinguish cancer cells from other unhealthy or healthy cells in complex biological systems. Herein, we developed a smart, two-photon fluorescent GC-NABP nanoprobe with pH-dependent surface charge conversion for tumor-targeted imaging of H2O2. The nanoprobe was constructed by the self-assembly of amphiphilic GC-NABP, which was synthesized by grafting the hydrophobic, H2O2-responsive and two-photon fluorophore, NABP, onto hydrophilic biopolymer glycol chitosan (GC). Taking advantage of pH-titratable amino groups on GC, the nanoprobe had the capability of surface charge conversion from negative at physiologic pH to positive in the acidic tumor microenvironment. The positive charge of the nanoprobe promoted electrostatic interactions with cell membranes, leading to enhanced cellular uptake in acidic environment. Upon cellular uptake, the high level of H2O2 in tumor cells triggered boronate deprotections of the nanoprobe, generating a "turn-on" fluorescence emission for H2O2 imaging. The nanoprobe exhibited good sensitivity and selectivity to H2O2 with a detection limit down to 110 nM in vitro. The results from flow cytometry and two-photon fluorescence imaging of H2O2 in living cells and tissues evidenced the enhanced cellular uptake and targeted imaging of intracellular H2O2 in acidic environment. Compared to control nanoparticles that lack pH sensitivity, our nanoprobe showed enhanced accumulation in tumor sites and was applied to targeted imaging of H2O2 in a tumor-bearing mouse model. This work demonstrates that the nanoprobe GC-NABP holds great promise for tumor-specific imaging of cellular H2O2, providing a potential tool to explore the role of H2O2 in tumor sites.
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| GB/T 7714 | Chen, Lanlan , Xu, Shuai , Li, Wei et al. Tumor-acidity activated surface charge conversion of two-photon fluorescent nanoprobe for enhanced cellular uptake and targeted imaging of intracellular hydrogen peroxide [J]. | CHEMICAL SCIENCE , 2019 , 10 (40) : 9351-9357 . |
| MLA | Chen, Lanlan et al. "Tumor-acidity activated surface charge conversion of two-photon fluorescent nanoprobe for enhanced cellular uptake and targeted imaging of intracellular hydrogen peroxide" . | CHEMICAL SCIENCE 10 . 40 (2019) : 9351-9357 . |
| APA | Chen, Lanlan , Xu, Shuai , Li, Wei , Ren, Tianbing , Yuan, Lin , Zhang, Shusheng et al. Tumor-acidity activated surface charge conversion of two-photon fluorescent nanoprobe for enhanced cellular uptake and targeted imaging of intracellular hydrogen peroxide . | CHEMICAL SCIENCE , 2019 , 10 (40) , 9351-9357 . |
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