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学者姓名：郭良洽
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Abstract ：
Monitoring mitochondrial pH and differentiating live and dead cells are crucial for diagnosing cell status. However, most fluorescent probes suffer from limitations such as high cytotoxicity, photobleaching, unreliability, and an inability to differentiate cell death caused by different inducers. Herein, a ratiometric fluorescent nanoprobe was developed by assembling pH-sensitive hydroxyl- and phenyl-co-modified carbon nitride (HPCN) with pH-insensitive Rhodamine B (RB). HPCN was prepared via thermal condensation of phenylguanidine carbonate using NaOH as the melt. The hydroxyl group modification endowed HPCN with improved water solubility and pH-sensitive characteristics, while the phenyl group modification facilitated mitochondrial targeting and DNA staining via hydrophobic interactions. Based on the fluorescence resonance energy transfer (FRET) from HPCN to RB, the nanoprobe exhibited a linear response in the relative fluorescence intensities at 500 nm and 584 nm over a pH range of 4.5-8.5. Benefiting from its low cytotoxicity, excellent reversibility, and outstanding photostability, the nanoprobe was capable of monitoring mitochondrial pH changes in live cells and differentiating live and dead cells, apoptosis and necrosis, and necrosis induced by different agents, regardless of cell type. This work provides a reliable method for diagnosing cell status and cell death induced by various inducers.
Keyword ：
Carbon nitride Carbon nitride Cell differentiation Cell differentiation Mitochondrial pH Mitochondrial pH Nanoprobe Nanoprobe Ratiometric fluorescence Ratiometric fluorescence
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| GB/T 7714 | Chen, Jingru , Zhang, Xiaomin , Lu, Heng et al. Hydroxyl and phenyl co-modified carbon nitride-based ratiometric fluorescent nanoprobe for monitoring mitochondrial pH in live cells and differentiating cell death [J]. | TALANTA , 2025 , 291 . |
| MLA | Chen, Jingru et al. "Hydroxyl and phenyl co-modified carbon nitride-based ratiometric fluorescent nanoprobe for monitoring mitochondrial pH in live cells and differentiating cell death" . | TALANTA 291 (2025) . |
| APA | Chen, Jingru , Zhang, Xiaomin , Lu, Heng , Wu, Yali , Xu, Yuanteng , Guo, Liangqia . Hydroxyl and phenyl co-modified carbon nitride-based ratiometric fluorescent nanoprobe for monitoring mitochondrial pH in live cells and differentiating cell death . | TALANTA , 2025 , 291 . |
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The cascaded catalytic reactions of glucose oxidase (GOx) and horseradish peroxidase (HRP)-like nanozymes have been extensively used for the colorimetric detection of serum glucose. Nevertheless, the usage of biological enzyme and the adjustment of buffer pH due to the inconsistent pH of this two-step catalytic reactions are involved during the detection of serum samples. Herein, the photocatalysis of cyanamide-functionalized carbon nitride (CACN) with high efficient electron-hole separation and photoelectron storage ability was utilized for non-enzymatic colorimetric sensing of serum glucose without the need to adjust the buffer pH and the usage of biological enzyme. Under the irradiation of visible light, glucose was utilized as electron donor to quench the photogenerated holes of CACN and to facilitate the reduction of 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2Htetrazolium-5-carboxanilide (XTT) by the photoelectrons into orange-yellow product (formazan). The absorbance of formazan at 470 nm showed a linear response toward glucose over 1.8-22.0 mmol L- 1 with the limit of detection of 0.27 mmol L- 1 (S/N = 3). Furthermore, the digital image colorimetric (DIC) method was also developed by a portable mobile. Finally, the colorimetric and DIC methods were utilized for the detection of serum glucose with high accuracy, reproducibility and recovery. This work not only proposed a new strategy for the colorimetric sensing of serum glucose but also extended the photocatalysis of carbon nitride application in the field of biosensing.
Keyword ：
Carbon nitride Carbon nitride Colorimetric sensing Colorimetric sensing Digital image colorimetry Digital image colorimetry Photocatalysis Photocatalysis Serum glucose Serum glucose
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| GB/T 7714 | Cui, Mei , Zheng, Wanling , Zhang, Xiaomin et al. Cyanamide-functionalized carbon nitride based photocatalysis for non-enzymatic colorimetric sensing of serum glucose [J]. | TALANTA , 2025 , 293 . |
| MLA | Cui, Mei et al. "Cyanamide-functionalized carbon nitride based photocatalysis for non-enzymatic colorimetric sensing of serum glucose" . | TALANTA 293 (2025) . |
| APA | Cui, Mei , Zheng, Wanling , Zhang, Xiaomin , Chen, Jingru , Xu, Yuanteng , Guo, Liangqia . Cyanamide-functionalized carbon nitride based photocatalysis for non-enzymatic colorimetric sensing of serum glucose . | TALANTA , 2025 , 293 . |
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It is of vital significance to explore effective and economical multifunctional materials for the detection, remediation and recycling of heavy metal (Cr(VI)). Herein, two-dimensional (2D) phenyl -modified carbon nitride (PCN) polymer was prepared through thermal polymerization of phenylguanidine carbonate. The incorporation of phenyl groups endowed PCN polymer with both strong green fluorescence and high photocatalytic activity for the reduction of Cr(VI). An multifunctional gel for simultaneous detection, adsorption, photo -reduction and recycling of Cr(VI) was further prepared by assembling PCN with polyethyleneimine-N,N'-methyl- enebisacrylamide (PEI -MBA) gel. PCN-PEI-MBA gel showed a sensitive fluorescence / colorimetric dual -mode detection of Cr(VI). Meanwhile, PCN-PEI-MBA gel displayed preeminent adsorption capacity of 3055.9 mg g-1 MBA gel was photocatalytically reduced into Cr(III) with visual real-time monitoring the detoxification process and Cr(III) can be further recycled as Cr2O3 by calcination of PCN-PEI-MBA gel. Finally, PCN-PEI-MBA gel was utilize to adsorb and recycle Cr(VI) from the chromium -plating wastewater and detect the concentration of remaining Cr(VI) in the wastewater. This work provides an all -in -one treatment strategy for the detection, remediation and resource recycling of Cr(VI) in practical wastewater. , surpassing previously reported adsorbents. Under visible -light irradiation, the absorbed Cr(VI) in PCN-PEI
Keyword ：
Adsorption Adsorption Carbon nitride Carbon nitride Chromium ion Chromium ion Chromium recycling Chromium recycling Detection Detection Photocatalytic reduction Photocatalytic reduction
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| GB/T 7714 | Chen, Jingru , Qiu, Yonghan , Liu, Xiaotao et al. Phenyl-modified carbon nitride based multifunctional gel for the detection, adsorption, photocatalytic reduction and recycling of chromium(VI) in wastewater [J]. | APPLIED SURFACE SCIENCE , 2024 , 652 . |
| MLA | Chen, Jingru et al. "Phenyl-modified carbon nitride based multifunctional gel for the detection, adsorption, photocatalytic reduction and recycling of chromium(VI) in wastewater" . | APPLIED SURFACE SCIENCE 652 (2024) . |
| APA | Chen, Jingru , Qiu, Yonghan , Liu, Xiaotao , Guo, Liangqia . Phenyl-modified carbon nitride based multifunctional gel for the detection, adsorption, photocatalytic reduction and recycling of chromium(VI) in wastewater . | APPLIED SURFACE SCIENCE , 2024 , 652 . |
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Fluorescent labeling and imaging is a useful tool to track the temporal and spatial distribution of microplastics (MPs) with size and shape features. However, the limited fluorescently labeled MPs spheres, photo -bleaching and leaching of organic small molecular dyes hamper the tracking of MPs in the environment. In this work, phenyl -modified carbon nitride (PMCN) fluorescent polymer was prepared and applied for the staining of eight different types of MPs. The interface interaction mechanism between PMCN fluorescent polymer and MPs was investigated by the desorption experiments. The long-time photo -stability of the stained MPs against ultraviolet (UV) light irradiation, freeze -thaw cycles, various environmental factors and real environmental water samples was evaluated. The MPs stained with PMCN fluorescent polymer showed more robust stability than the common dye (Nile red) against the damage when exposure to UV light for 72 h, treatment with freeze -thaw cycles for 60 days, exposure to strong acid, strong alkali, high salinity, freshwater (river and lake) or seawater for 60 days. Results indicate PMCN fluorescent polymer is promising as a convenient, efficient and universal fluorescent tracer for visually tracking the migration and distribution of MPs in environmental water system.
Keyword ：
Environmental water Environmental water Fluorescence image Fluorescence image Microplastics Microplastics Migration Migration Tracking Tracking
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| GB/T 7714 | Feng, Qiaocheng , Cui, Mei , Chen, Jingru et al. Enhanced long-term stability of stained microplastics with carbon nitride fluorescent polymer for tracking [J]. | ENVIRONMENTAL TECHNOLOGY & INNOVATION , 2024 , 34 . |
| MLA | Feng, Qiaocheng et al. "Enhanced long-term stability of stained microplastics with carbon nitride fluorescent polymer for tracking" . | ENVIRONMENTAL TECHNOLOGY & INNOVATION 34 (2024) . |
| APA | Feng, Qiaocheng , Cui, Mei , Chen, Jingru , Zheng, Xueyi , Guo, Liangqia . Enhanced long-term stability of stained microplastics with carbon nitride fluorescent polymer for tracking . | ENVIRONMENTAL TECHNOLOGY & INNOVATION , 2024 , 34 . |
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Typical fluorescent biosensors use fluorescently labeled ssDNA for target recognition and nanomaterials for signal transduction. Herein, we propose a reverse sensing strategy that Mo5N6 nanosheets are used for target recognition while fluorescein (FAM)-labeled ssDNA only serves for signal generation. We discover that Mo5N6 nanosheets show high fluorescence quenching ability (>95%) and selective recognition for sodium hexametaphosphate (SHMP). After FAM-labeled ssDNA is adsorbed on Mo5N6 nanosheets, the fluorescence is quenched due to the photoinduced electron transfer (PET) effect between FAM and Mo5N6 nanosheets. SHMP can specifically displace the adsorbed FAM-labeled ssDNA from Mo5N6 nanosheets, resulting in more than 80% fluorescence recovery on addition of 5 mu mol L-1 SHMP. This biosensor can sensitively detect SHMP down to 150 nmol L-1 and selectively recognize SHMP over glucose, lactose, common amino acids, Zn2+, Mg2+, Ca2+ and other phosphates (such as Na2HPO4, sodium pyrophosphate, sodium tripolyphosphate). This biosensor also shows great potential for the detection of SHMP in bacon sample. This work not only provides a facile sensitive and selective biosensor for SHMP but also exploits the application of transition metal nitrides in the field of sensing and biosensing.
Keyword ：
Biosensor Biosensor Food safety Food safety Molybdenum nitrides Molybdenum nitrides Sodium hexametaphosphate Sodium hexametaphosphate Turn-on Turn-on
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| GB/T 7714 | Huang, Wenying , Wu, Yali , Xia, Chunqiu et al. Mo5N6 nanosheets for fluorescent quenching and target recognition: Highly selectively sensing of sodium hexametaphosphate [J]. | TALANTA , 2024 , 273 . |
| MLA | Huang, Wenying et al. "Mo5N6 nanosheets for fluorescent quenching and target recognition: Highly selectively sensing of sodium hexametaphosphate" . | TALANTA 273 (2024) . |
| APA | Huang, Wenying , Wu, Yali , Xia, Chunqiu , Guo, Liangqia . Mo5N6 nanosheets for fluorescent quenching and target recognition: Highly selectively sensing of sodium hexametaphosphate . | TALANTA , 2024 , 273 . |
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CD39 is one of the important nucleotidases to adjust extracellular adenosine triphosphate (ATP) and adenosine diphosphate (ADP) concentration. However, the enzyme mimics to simulate the activity of CD39 still remains to be explored. Herein nitrogen-rich molybdenum nitride (Mo5N6) nanosheets are explored to possess CD39-like activity, which are able to catalyze the hydrolysis of the high-energy phosphate bonds (HEPBs) in ATP and ADP but not the common phosphate bonds in adenosine monophosphate (AMP). The catalytic hydrolysis of the phosphate bond over Mo5N6-700 nanosheets is first investigated using para-nitrophenyl phosphate as the model substrate and then the CD39-like activity is further explored and verified by (31)p NMR spectroscopy. Mo4+ on the surface of Mo5N6-700 nanosheets are the catalytic active sites. Using ATP as the model substrate, the K-m and V-max values of CD39-like activity at optimal pH 9.0 are 3.2 mu mol L-1 and 18.5 mu mol L-1 h(-1), respectively. The CD39-like activity of Mo5N6-700 nanosheets enabled the down-regulation of intracellular ATP concentration to a larger degree for cancer cells than normal cells, which makes Mo5N6-700 nanosheets a potential therapeutic reagent for cancers.
Keyword ：
ATP ATP CD39 CD39 Mo5N6 Mo5N6 nucleotidase nucleotidase phosphatase phosphatase
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| GB/T 7714 | Zhang, Xiaomin , Xia, Chunqiu , Guo, Liangqia . Nitrogen-Rich Molybdenum Nitride with Intrinsic CD39 Nucleotidase Activity [J]. | SMALL , 2024 , 21 (2) . |
| MLA | Zhang, Xiaomin et al. "Nitrogen-Rich Molybdenum Nitride with Intrinsic CD39 Nucleotidase Activity" . | SMALL 21 . 2 (2024) . |
| APA | Zhang, Xiaomin , Xia, Chunqiu , Guo, Liangqia . Nitrogen-Rich Molybdenum Nitride with Intrinsic CD39 Nucleotidase Activity . | SMALL , 2024 , 21 (2) . |
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Herein, oxidized 3,3 ',5,5 '-tetramethylbenzidine (oxTMB) nanobelts were developed to enhance the colorimetric and paper-based sensing of H2O2. It was found that the minor component of Fe2+ in Na2SO4 reagent could catalyze the oxidization of TMB by H2O2 into positively charged oxTMB, which was further assembled into dark blue oxTMB nanobelts via electrostatic interaction with SO42- . The extinction originating from the absorption and scattering of oxTMB nanobelts was utilized to quantitatively detect H2O2 with a wide linear detection range (1.0-300 mu M) and a low limit of detection (0.48 mu M). In addition, no coffee-ring effect was observed in the test zone of the paper-based colorimetric array, which was beneficial to judge the color by naked eye. Finally, the colorimetric method was applied to detect H2O2 in contact lens care solution. This work not only proposed a new colorimetric sensing platform for H2O2, but also highlighted the minor component in the reagent might influence the experimental result.
Keyword ：
Assembly Assembly Colorimetric sensor Colorimetric sensor Fe 2+ Fe 2+ Na2SO4 Na2SO4 Nanobelts Nanobelts TMB TMB
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| GB/T 7714 | Xu, Lanlan , Yang, Bingyu , Guo, Liangqia . Oxidized 3,3′,5,5′-tetramethylbenzidine nanobelts enhance colorimetric sensing of H2O2 [J]. | TALANTA , 2024 , 279 . |
| MLA | Xu, Lanlan et al. "Oxidized 3,3′,5,5′-tetramethylbenzidine nanobelts enhance colorimetric sensing of H2O2" . | TALANTA 279 (2024) . |
| APA | Xu, Lanlan , Yang, Bingyu , Guo, Liangqia . Oxidized 3,3′,5,5′-tetramethylbenzidine nanobelts enhance colorimetric sensing of H2O2 . | TALANTA , 2024 , 279 . |
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MicroRNAs (miRNAs) are pivotal in regulating biological processes such as cell proliferation and disease progression. Traditional miRNA detection methods like qRT-PCR and Northern blotting do not allow for monitoring dynamic changes in living cells and typically require invasive sample collection. This research presents a robust, non-enzymatic technique known as the Localized Hybridization Chain Reaction Amplifier (LHCRA), designed for real-time, in vivo miRNA imaging. This method addresses these limitations by providing rapid and sensitive detection of miRNAs, crucial for progressing clinical diagnostics and research. The LHCRA utilizes hairpin chain reaction probes embedded within self-assembled DNA micelles (DM), significantly amplifying miRNA signals. LHCRA demonstrated exceptional performance, with a detection limit of 100 pM and a response time of 10 min. Importantly, it effectively differentiated between cancerous and normal cells using clinical peripheral blood samples, showcasing high specificity and stability under physiological conditions. Additionally, LHCRA maintained consistent performance in complex biological media, including 10 % serum and 2 U/mL DNase I, confirming its broad applicability in various diagnostic scenarios. This performance highlights LHCRA's potential as a transformative tool in miRNA-based diagnostics. The introduction of LHCRA marks a significant advancement in miRNA detection technology. By enabling accurate, non-invasive, and real-time monitoring of miRNA dynamics within living cells, this method offers substantial potential to enhance diagnostic accuracy and deepen our understanding of disease mechanisms, particularly in cancer. Thus, it could greatly improve therapeutic strategies and patient outcomes in clinical environment.
Keyword ：
Cancer diagnostics Cancer diagnostics DNA nanostructures DNA nanostructures In situ monitoring In situ monitoring Living cell imaging Living cell imaging microRNA microRNA Non-enzymatic amplification Non-enzymatic amplification
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| GB/T 7714 | Yang, Bingyu , Gao, Xiang , Yu, Hengxin et al. Localized hybridization chain reaction amplifier utilizing three-dimensional DNA nanostructures for efficient and reliable microRNA imaging in living cells [J]. | ANALYTICA CHIMICA ACTA , 2024 , 1330 . |
| MLA | Yang, Bingyu et al. "Localized hybridization chain reaction amplifier utilizing three-dimensional DNA nanostructures for efficient and reliable microRNA imaging in living cells" . | ANALYTICA CHIMICA ACTA 1330 (2024) . |
| APA | Yang, Bingyu , Gao, Xiang , Yu, Hengxin , Xu, Jiawei , Liu, Wenming , Xu, Huo et al. Localized hybridization chain reaction amplifier utilizing three-dimensional DNA nanostructures for efficient and reliable microRNA imaging in living cells . | ANALYTICA CHIMICA ACTA , 2024 , 1330 . |
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Nanozymes have attracted great interest in the field of biotechnology, (bio)sensing, and environmental monitoring. Herein, a co-doping strategy is proposed to enhance the peroxidase-like activity of graphitic carbon nitride (g-C3N4). Cu2+ and triazole group co-doped graphitic carbon nitride (g-C3N5-Cu2+) was derived from thermal polymerization of 3-amino-1,2,4-triazole and then coordination with Cu2+. g-C3N5Cu2+ nanosheets (NSs) could catalyze the oxidation reaction of 3,3′,5,5′-tetramethylbenzidine (TMB) by H2O2 under neutral conditions, yielding 10.9-fold better catalytic activity than g-C3N4 NSs. Reactive oxygen species scavenging experiments indicated that ·OH played a crucial role during the catalytic process. When glutathione (GSH) was introduced, the absorbance of the system decreased because of the reduction of the oxidation product of TMB by GSH. The colorimetric method demonstrated a sensitive response for GSH with a linear range of 0.8–33.3 μmol L-1 and a limit of detection of 0.3 μmol L-1. The sensitivity is higher than most other peroxidase mimetic-based colorimetric methods for GSH. Finally, the feasibility for the detection of GSH in HeLa cells was demonstrated. © 2023 The Author(s).
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| GB/T 7714 | Liu, X. , Zheng, X. , Xia, C. et al. Graphitic carbon nitride with Cu2+ and triazole group co-doping for enhanced peroxidase-like activity and its application for glutathione detection† [J]. | Sensors and Diagnostics , 2023 , 2 (4) : 902-908 . |
| MLA | Liu, X. et al. "Graphitic carbon nitride with Cu2+ and triazole group co-doping for enhanced peroxidase-like activity and its application for glutathione detection†" . | Sensors and Diagnostics 2 . 4 (2023) : 902-908 . |
| APA | Liu, X. , Zheng, X. , Xia, C. , Guo, L. . Graphitic carbon nitride with Cu2+ and triazole group co-doping for enhanced peroxidase-like activity and its application for glutathione detection† . | Sensors and Diagnostics , 2023 , 2 (4) , 902-908 . |
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Herein, a vinylene-linked covalent organic framework (TMT-BPDA-COF) was prepared via the Aldol condensation of 2,4,6-trimethyl-1,3,5-triazine (TMT) and 4,4'-biphenyl dimethyl formaldehyde (BPDA). TMT-BPDACOF displayed high crystallinity and thermal stability, and robust chemical stability in strong acid, strong base and common organic solvents. TMT-BPDA-COF nanosheets emitted strong yellow fluorescence with the quantum yield of 14.9% and showed fast reversible response toward pH with a wide range (pH 0.0 - 14.0) based on the intramolecular charge transfer effect. Furthermore, portable fluorescent sensors were prepared by incorporated TMT-BPDA-COF nanosheets into agarose gel and cellulose acetate butyrate (CAB), respectively. TMT-BPDA-COF gel showed a wide pH response range over pH 0.0 - 14.0 with a linear range of pH 4.0 - 14.0. TMT-BPDA-COF CAB film exhibited high sensitivity for sensing of NH3 gas with a limit of detection of 1.6 ppb. TMT-BPDA-COF agarose gel and CAB film also exhibited good reversibility and reproducibility in response to pH and NH3 gas, respectively. We expect TMT-BPDA-COF-based sensors, which feature fast response, high sensitivity, wide response range, good reproducibility and reversibility, can apply to detect pH and NH3 gas in the field of environment and industry.
Keyword ：
Covalent organic frameworks Covalent organic frameworks Films Films Fluorescent sensors Fluorescent sensors Gels Gels NH3 NH3 PH PH
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| GB/T 7714 | Cai, Jiaming , Qiu, Yonghan , Qi, Guomin et al. A Highly fluorescent vinylene-linked covalent organic framework for chemical sensing of pH and NH3 gas with wide dynamic range [J]. | SENSORS AND ACTUATORS B-CHEMICAL , 2023 , 402 . |
| MLA | Cai, Jiaming et al. "A Highly fluorescent vinylene-linked covalent organic framework for chemical sensing of pH and NH3 gas with wide dynamic range" . | SENSORS AND ACTUATORS B-CHEMICAL 402 (2023) . |
| APA | Cai, Jiaming , Qiu, Yonghan , Qi, Guomin , Guo, Liangqia . A Highly fluorescent vinylene-linked covalent organic framework for chemical sensing of pH and NH3 gas with wide dynamic range . | SENSORS AND ACTUATORS B-CHEMICAL , 2023 , 402 . |
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