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学者姓名：吴韶华
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Abstract ：
The flap endonuclease 1 (FEN1) plays a key role in DNA replication and repair, its aberrant expression is associated with tumor development, so it has been recognized as a promising biomarker for a variety of cancers. Here, a novel "turn on" mode gold nanocube-enhanced surface-enhanced Raman scattering (SERS) biosensor was constructed by combining a heated Au electrode (HAuE), exonuclease III (Exo III)-assisted cycle amplification, and gold nanocube (AuNC)-based SERS enhancement to achieve highly sensitive detection of FEN1 activity. The SERS tag was prepared using the Raman reporter modified on the AuNC surface, and the high electromagnetic field provided by the sharp geometric feature of AuNC greatly enhanced the SERS signal. At the same time, HAuE was used to increase the electrode surface temperature and enhance the FEN1 activity, leading to more trigger DNA being cleaved, which was used to initiate the Exo III-assisted cycle amplification. Taking all these advantages, the proposed method possessed high sensitivity and good selectivity, with a low limit of detection (LOD) of 3.19 x 10- 7 U mu L- 1. In addition, this method was successfully applied to detect FEN1 activity in real cellular extracts.
Keyword ：
Exo III-Assisted cycle amplification Exo III-Assisted cycle amplification Flap endonuclease 1 (FEN1) Flap endonuclease 1 (FEN1) Heated Au electrode Heated Au electrode Surface enhanced Raman spectroscopy (SERS) Surface enhanced Raman spectroscopy (SERS)
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| GB/T 7714 | Jin, Lei-Xin , Wang, Qi-Meng , Zhu, Shu-Jiao et al. Gold nanocube-enhanced SERS biosensor based on heated electrode coupled with exonuclease III-assisted cycle amplification for sensitive detection of flap endonuclease 1 activity [J]. | TALANTA , 2025 , 286 . |
| MLA | Jin, Lei-Xin et al. "Gold nanocube-enhanced SERS biosensor based on heated electrode coupled with exonuclease III-assisted cycle amplification for sensitive detection of flap endonuclease 1 activity" . | TALANTA 286 (2025) . |
| APA | Jin, Lei-Xin , Wang, Qi-Meng , Zhu, Shu-Jiao , Zhu, Cai-Lian , Sun, Jian-Jun , Wu, Shao-Hua . Gold nanocube-enhanced SERS biosensor based on heated electrode coupled with exonuclease III-assisted cycle amplification for sensitive detection of flap endonuclease 1 activity . | TALANTA , 2025 , 286 . |
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Real-time, high-frequency measurements of pharmaceuticals, metabolites, exogenous antigens, and other biomolecules in biological samples can provide critical information for health management and clinical diagnosis. Electrochemical aptamer-based (EAB) sensor is a promising analytical technique capable of achieving these goals. However, the issues of insufficient sensitivity, frequent calibration and lack of adapted portable electrochemical device limit its practical application in immediate detection. In response we have fabricated an on-chip-integrated, cold-hot Janus EAB (J-EAB) sensor based on the thermoelectric coolers (TECs). Attributed to the Peltier effect, the enhanced/suppressed current response can be generated simultaneously on cold/hot sides of the J-EAB sensor. The ratio of the current responses on the cold and hot sides was used as the detection signal, enabling rapid on-site, calibration-free determination of small molecules (procaine) as well as macromolecules (SARS-CoV-2 spike protein) in single step, with detection limits of 1 μM and 10 nM, respectively. We have further demonstrated that the J-EAB sensor is effective in improving the ease and usability of the actual detection process, and is expected to provide a universal, low-cost, fast and easy potential analytical tool for other clinically important biomarkers, drugs or pharmaceutical small molecules. © 2024 Elsevier B.V.
Keyword ：
Calibration-free Calibration-free Cold-hot Cold-hot Electrochemical aptamer-based sensor Electrochemical aptamer-based sensor Janus Janus Peltier effect Peltier effect
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| GB/T 7714 | Feng, L. , Gao, R.-Y. , Chen, Z.-M. et al. Cold-hot Janus electrochemical aptamer-based sensor for calibration-free determination of biomolecules [J]. | Biosensors and Bioelectronics , 2024 , 264 . |
| MLA | Feng, L. et al. "Cold-hot Janus electrochemical aptamer-based sensor for calibration-free determination of biomolecules" . | Biosensors and Bioelectronics 264 (2024) . |
| APA | Feng, L. , Gao, R.-Y. , Chen, Z.-M. , Qin, S.-N. , Cao, Y.-J. , Salminen, K. et al. Cold-hot Janus electrochemical aptamer-based sensor for calibration-free determination of biomolecules . | Biosensors and Bioelectronics , 2024 , 264 . |
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Abstract ：
Real-time, high-frequency measurements of pharmaceuticals, metabolites, exogenous antigens, and other biomolecules in biological samples can provide critical information for health management and clinical diagnosis. Electrochemical aptamer-based (EAB) sensor is a promising analytical technique capable of achieving these goals. However, the issues of insufficient sensitivity, frequent calibration and lack of adapted portable electrochemical device limit its practical application in immediate detection. In response we have fabricated an on-chip-integrated, cold-hot Janus EAB (J-EAB) sensor based on the thermoelectric coolers (TECs). Attributed to the Peltier effect, the enhanced/suppressed current response can be generated simultaneously on cold/hot sides of the J-EAB sensor. The ratio of the current responses on the cold and hot sides was used as the detection signal, enabling rapid on-site, calibration-free determination of small molecules (procaine) as well as macromolecules (SARS-CoV-2 spike protein) in single step, with detection limits of 1 mu M and 10 nM, respectively. We have further demonstrated that the J-EAB sensor is effective in improving the ease and usability of the actual detection process, and is expected to provide a universal, low-cost, fast and easy potential analytical tool for other clinically important biomarkers, drugs or pharmaceutical small molecules.
Keyword ：
Calibration-free Calibration-free Cold-hot Cold-hot Electrochemical aptamer-based sensor Electrochemical aptamer-based sensor Janus Janus Peltier effect Peltier effect
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| GB/T 7714 | Feng, Lei , Gao, Run-Yu , Chen, Zhi-Min et al. Cold-hot Janus electrochemical aptamer-based sensor for calibration-free determination of biomolecules [J]. | BIOSENSORS & BIOELECTRONICS , 2024 , 264 . |
| MLA | Feng, Lei et al. "Cold-hot Janus electrochemical aptamer-based sensor for calibration-free determination of biomolecules" . | BIOSENSORS & BIOELECTRONICS 264 (2024) . |
| APA | Feng, Lei , Gao, Run-Yu , Chen, Zhi-Min , Qin, Sai-Nan , Cao, Yi-Jie , Salminen, Kalle et al. Cold-hot Janus electrochemical aptamer-based sensor for calibration-free determination of biomolecules . | BIOSENSORS & BIOELECTRONICS , 2024 , 264 . |
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本发明公开一种检测DNA甲基转移酶活性的SERS传感器。该传感器包括金盘温控电极，金电极，限制性内切酶DpnI，聚合酶KFP，切口核酸内切酶Nb.BbvcI，基底探针H1，引发探针triDNA，捕获探针H2，信号探针sDNA和信号分子4‑MBA组装在金纳米立方块表面的SERS tag。通过提高温控金电极的表面温度，Dam MTase和DpnI的活性显着增加，从而导致SDA模板DNA的快速产生。在SDA过程中，释放的ssDNA呈指数级扩增，其浓度与Dam MTase的活性呈正相关。本发明检测限为8.65×10‑5 U mL‑1，低于大多数文献报道值。
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| GB/T 7714 | 吴韶华 , 胡浩成 . 一种检测DNA甲基转移酶活性的SERS传感器 : CN202210289749.7[P]. | 2022-03-23 00:00:00 . |
| MLA | 吴韶华 et al. "一种检测DNA甲基转移酶活性的SERS传感器" : CN202210289749.7. | 2022-03-23 00:00:00 . |
| APA | 吴韶华 , 胡浩成 . 一种检测DNA甲基转移酶活性的SERS传感器 : CN202210289749.7. | 2022-03-23 00:00:00 . |
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An ultrasensitive electrochemical biosensor for detecting p53 gene was fabricated based on heated gold disk electrode coupling with endonuclease Nt.BstNBI-assisted target recycle amplification and alkaline phosphatase (ALP)-based electrocatalytic signal amplification. For biosensor assembling, biotinylated ssDNA capture probes were first immobilized on heated Au disk electrode (HAuDE), then combined with streptavidin-alkaline phosphatase (SA-ALP) by biotin-SA interaction. ALP could catalyze the hydrolysis of ascorbic acid 2-phosphate (AAP) to produce ascorbic acid (AA). While AA could induce the redox cycling to generate electrocatalytic oxidation current in the presence of ferrocene methanol (FcM). When capture probes hybridized with p53, Nt.BstNBI would recognize and cleave the duplexes and p53 was released for recycling. Meanwhile, the biotin group dropt from the electrode surface and subsequently SA-ALP could not adhere to the electrode. The signal difference before and after cleavage was proportional to the p53 gene concentration. Furthermore, with electrode temperature elevated, the Nt.BstNBI and ALP activities could be increased, greatly improving the sensitivity and efficiency for p53 detection. A detection limit of 9.5 x 10-17 M could be obtained (S/N = 3) with an electrode temperature of 40 degrees C, ca. four magnitudes lower than that at 25 degrees C.
Keyword ：
Alkaline phosphatase (ALP) Alkaline phosphatase (ALP) BstNBI BstNBI Electrochemical sensor Electrochemical sensor Heated gold disk electrode Heated gold disk electrode Nicking endonuclease Nicking endonuclease Nt Nt p53 gene p53 gene
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| GB/T 7714 | Mi, Zhen-Zhen , Hu, Hao-Cheng , Sun, Jian-Jun et al. Heating promoted super sensitive electrochemical detection of p53 gene based on alkaline phosphatase and nicking endonuclease Nt.BstNBI-assisted target recycling amplification strategy at heated gold disk electrode [J]. | ANALYTICA CHIMICA ACTA , 2023 , 1275 . |
| MLA | Mi, Zhen-Zhen et al. "Heating promoted super sensitive electrochemical detection of p53 gene based on alkaline phosphatase and nicking endonuclease Nt.BstNBI-assisted target recycling amplification strategy at heated gold disk electrode" . | ANALYTICA CHIMICA ACTA 1275 (2023) . |
| APA | Mi, Zhen-Zhen , Hu, Hao-Cheng , Sun, Jian-Jun , Wu, Shao-Hua . Heating promoted super sensitive electrochemical detection of p53 gene based on alkaline phosphatase and nicking endonuclease Nt.BstNBI-assisted target recycling amplification strategy at heated gold disk electrode . | ANALYTICA CHIMICA ACTA , 2023 , 1275 . |
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Abstract ：
本文以有机化学实验教学改革为例介绍了福州大学以培养学生的科学思维、科学能力和科学素养为核心的"C3H3"化学创新人才培养模式.通过夯实基础、以赛促改、科研反哺教学、更新实验内容,采用问题导向的实验教学方法,综合性的实验教学评价方法,全方位培养学生的创新意识和批判性思维,从而将实验教学和拔尖创新人才培养目标紧密结合起来.
Keyword ：
"C3H3"教育理念 "C3H3"教育理念 实验教学改革 实验教学改革 有机化学实验 有机化学实验
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| GB/T 7714 | 林彩霞 , 鄢剑锋 , 袁耀锋 et al. 基于"C3H3"教育理念的有机化学实验教学改革实践 [J]. | 高等理科教育 , 2022 , (5) : 32-40 . |
| MLA | 林彩霞 et al. "基于"C3H3"教育理念的有机化学实验教学改革实践" . | 高等理科教育 5 (2022) : 32-40 . |
| APA | 林彩霞 , 鄢剑锋 , 袁耀锋 , 林佳丽 , 吴韶华 . 基于"C3H3"教育理念的有机化学实验教学改革实践 . | 高等理科教育 , 2022 , (5) , 32-40 . |
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A novel split-type and signal-on photoelectrochemical (PEC) biosensor was proposed based on double-strand specific nuclease (DSN)-assisted recycling amplification coupled with photodeposited Ag enhanced BiVO4 photoanode for sensitive detection of microRNA (miRNA)-155. In the presence of target miRNA, the capture DNA (cDNA) modified with silver nanocube(AgNC)would hybridize with the miRNA, then DSN-assisted cycle amplification was triggered, leading to the release of AgNCs. The released AgNCs were acidolyzed and photodeposited on the BiVO4 electrode. Compared with the BiVO4 electrode, an enhanced photocurrent could be achieved at this obtained BiVO4/Ag electrode. And the morphology change from worm-like nanoparticles to nanosheets after Ag photodeposition was observed for the first time. This PEC signal enhancement was mainly contributed to the synergistic action of localized surface plasmon resonance (LSPR) effect of Ag, Schottky junction formed between BiVO4 and Ag, and the formation of BiVO4 nanosheets with larger specific surface area. The PEC signal enhancement was positively related to the miRNA-155 concentration, so this method could be used to sensitively and selectively detect miRNA-155. A limit of detection of 8 aM could be achieved. This strategy does not require layer-by-layer modification of the electrode, so it is easy to operate, and owns good stability.
Keyword ：
Biosensor, microRNA Biosensor, microRNA BiVO4/Ag BiVO4/Ag Duplex-strand specific nuclease (DSN) Duplex-strand specific nuclease (DSN) Photoelectrochemical Photoelectrochemical
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| GB/T 7714 | Wei, Yu-Yang , Wu, Shao-Hua , Wang, Qi-Meng et al. A novel split-type photoelectrochemical biosensor based on double-strand specific nuclease-assisted cycle amplification coupled with plasmonic Ag enhanced BiVO4 performance for sensitive detection of microRNA-155 [J]. | SENSORS AND ACTUATORS B-CHEMICAL , 2022 , 369 . |
| MLA | Wei, Yu-Yang et al. "A novel split-type photoelectrochemical biosensor based on double-strand specific nuclease-assisted cycle amplification coupled with plasmonic Ag enhanced BiVO4 performance for sensitive detection of microRNA-155" . | SENSORS AND ACTUATORS B-CHEMICAL 369 (2022) . |
| APA | Wei, Yu-Yang , Wu, Shao-Hua , Wang, Qi-Meng , Sun, Jian-Jun . A novel split-type photoelectrochemical biosensor based on double-strand specific nuclease-assisted cycle amplification coupled with plasmonic Ag enhanced BiVO4 performance for sensitive detection of microRNA-155 . | SENSORS AND ACTUATORS B-CHEMICAL , 2022 , 369 . |
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In this work, a novel "turn-on" mode Au nanocubes (AuNCs) enhanced surface-enhanced Raman scattering (SERS) biosensing platform coupled with heated Au electrode (HAuE) and strand displacement amplification (SDA) strategy was proposed for highly sensitive detection of DNA adenine methylation (Dam) Methyltransferase (MTase) activity. The Dam MTase and DpnI enzyme activities were significantly increased by elevating the HAuE surface temperature, resulting in the rapid production of template DNA for later SDA. During the SDA process, the released single-stranded DNA (ssDNA) could be amplified exponentially, and its concentration was positively related to the Dam MTase activity. The plasmonic AuNCs in SERS tags could provide significant SERS enhancement due to their "lightning rod" effect resulting from the sharp feature of the edges and corners of AuNCs. Because of these factors, the proposed biosensors exhibited high sensitivity in detecting the Dam MTase activity. The limit of detection was estimated to be 8.65 x 10(-5) U mL(-1), which was lower than that in most of the sensors for detection of Dam MTase activity in the literature. This SERS biosensor could also be used to screen inhibitors of Dam MTase and had the potential for detecting Dam MTase activity in real biological samples.
Keyword ：
Au nanocube Au nanocube Dam methyltransferase Dam methyltransferase Heated electrode Heated electrode Strand displacement amplification (SDA) Strand displacement amplification (SDA) Surface enhanced Raman spectroscopy (SEAS) Surface enhanced Raman spectroscopy (SEAS)
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| GB/T 7714 | Hu, Hao-Cheng , Wu, Shao-Hua , Jin, Lei-Xin et al. Plasmonic Au nanocube enhanced SERS biosensor based on heated electrode and strand displacement amplification for highly sensitive detection of Dam methyltransferase activity [J]. | BIOSENSORS & BIOELECTRONICS , 2022 , 210 . |
| MLA | Hu, Hao-Cheng et al. "Plasmonic Au nanocube enhanced SERS biosensor based on heated electrode and strand displacement amplification for highly sensitive detection of Dam methyltransferase activity" . | BIOSENSORS & BIOELECTRONICS 210 (2022) . |
| APA | Hu, Hao-Cheng , Wu, Shao-Hua , Jin, Lei-Xin , Sun, Jian-Jun . Plasmonic Au nanocube enhanced SERS biosensor based on heated electrode and strand displacement amplification for highly sensitive detection of Dam methyltransferase activity . | BIOSENSORS & BIOELECTRONICS , 2022 , 210 . |
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Surface-enhanced Raman scattering (SERS)-based biosensing has strong potential owing to its high sensitivity and good selectivity. In this study, a SERS biosensing platform for microRNA-21 detection was proposed by combining heating-enhanced duplex-specific nuclease (DSN)-assisted target recycling amplification and silver nanocube (AgNC)-based SERS enhancement. The activity of DSN was greatly enhanced by increasing the surface temperature of a heated Au electrode (HAuE) during the DSN-assisted target recycling process, resulting in dramatic miRNA-21 amplification detection. 4-Mercaptobenzoic acid (4-MBA) acting as a Raman reporter was immobilized onto the surface of AgNC to form a AgNC/4-MBA SERS tag with AgNC providing significantly larger electric field enhancement and thus excellent SERS applications compared with conventional silver or gold nanospheres. This enhancement was attributed to the well-known "lighting rod" effect derived from the sharp geometrical feature of the edges and corners of AgNCs. These factors lead to the high sensitivity of the proposed SERS biosensor for the miRNA-21 measurement. A limit of detection of 2.88 fM (S/N = 3) was achieved at an electrode temperature of 55 degrees C, which was decreased by more than three magnitudes compared with that at 25 degrees C.
Keyword ：
Ag nanocubes Ag nanocubes duplex-specific nuclease (DSN) duplex-specific nuclease (DSN) heated gold electrode heated gold electrode microRNA microRNA surface-enhanced Raman spectroscopy (SERS) surface-enhanced Raman spectroscopy (SERS)
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| GB/T 7714 | Du, Xing-Yuan , Wu, Shao-Hua , Huang, Xiao-Bin et al. Ag Nanocubes Coupled with Heating-Enhanced DSN-Assisted Cycling Amplification for Surface-Enhanced Raman Spectroscopy Detection of MicroRNA-21 [J]. | ACS APPLIED NANO MATERIALS , 2021 , 4 (3) : 2565-2574 . |
| MLA | Du, Xing-Yuan et al. "Ag Nanocubes Coupled with Heating-Enhanced DSN-Assisted Cycling Amplification for Surface-Enhanced Raman Spectroscopy Detection of MicroRNA-21" . | ACS APPLIED NANO MATERIALS 4 . 3 (2021) : 2565-2574 . |
| APA | Du, Xing-Yuan , Wu, Shao-Hua , Huang, Xiao-Bin , Sun, Jian-Jun . Ag Nanocubes Coupled with Heating-Enhanced DSN-Assisted Cycling Amplification for Surface-Enhanced Raman Spectroscopy Detection of MicroRNA-21 . | ACS APPLIED NANO MATERIALS , 2021 , 4 (3) , 2565-2574 . |
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Abstract ：
Surface-enhanced Raman scattering (SERS) biosensing has attracted great attention due to its multiplexed capability, high sensitivity, and rapid readout. In this study, plasmonic Ag nanocube (AgNC) enhanced SERS biosensing platform was proposed by coupling nicking endonuclease-assisted target recycling signal amplification with heated electrode for sensitive detection of DNA species related to oral cancer. SERS tags were manufactured by signal DNA (sDNA) conjugating onto the surfaces of AgNCs, and 4-mercaptobenzoic acid (4-MBA) assembled on the surfaces of AgNC/sDNA as a Raman reporter. The sharp feature of edges and corners of AgNCs with high electromagnetic field provided SER hot spots with high quality, and stronger SERS signal could be achieved. In addition, the activity of nicking endonuclease Nt.BstNBI could be greatly enhanced by elevating the temperature of the surface of the heated Au electrode (HAuE) during Nt.BstNBI-assisted target recycling amplification process, resulting in dramatic amplification of target DNA detection. All of these contributed to the high sensitivity of the developed SERS biosensor. A limit of detection (LOD) of 3.1 fM (S/N = 3) could be obtained with an electrode temperature of 45 ?C. This proposed SERS biosensor was anticipated to act as a promising biosensing platform for early clinical diagnosis of oral cancer.
Keyword ：
Ag nanocube Ag nanocube BstNBI BstNBI Heated electrode Heated electrode Nicking endonuclease signal amplification (NESA) Nicking endonuclease signal amplification (NESA) Nt Nt Oral cancer DNA Oral cancer DNA Surface-enhanced Raman spectroscopy (SERS) Surface-enhanced Raman spectroscopy (SERS)
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| GB/T 7714 | Liu, Yan , Wu, Shao-Hua , Du, Xing-Yuan et al. Plasmonic Ag nanocube enhanced SERS biosensor for sensitive detection of oral cancer DNA based on nicking endonuclease signal amplification and heated electrode [J]. | SENSORS AND ACTUATORS B-CHEMICAL , 2021 , 338 . |
| MLA | Liu, Yan et al. "Plasmonic Ag nanocube enhanced SERS biosensor for sensitive detection of oral cancer DNA based on nicking endonuclease signal amplification and heated electrode" . | SENSORS AND ACTUATORS B-CHEMICAL 338 (2021) . |
| APA | Liu, Yan , Wu, Shao-Hua , Du, Xing-Yuan , Sun, Jian-Jun . Plasmonic Ag nanocube enhanced SERS biosensor for sensitive detection of oral cancer DNA based on nicking endonuclease signal amplification and heated electrode . | SENSORS AND ACTUATORS B-CHEMICAL , 2021 , 338 . |
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