Query：
学者姓名：段建平
Refining：
Year
Type
Indexed by
Source
Complex
Former Name
Co-
Language
Clean All
Abstract ：
Two online sample stacking methods in capillary electrophoresis, large volume sample stacking using reversed pressure as a pump (LVSRP) and large volume sample stacking using electroosmotic flow (EOF) pump (LVSEP), were developed for the separation and determination of carnosine, anserine, and homocarnosine. The principles of the two stacking methods were described. Various parameters affecting capillary electrophoresis separation and sample stacking were studied and optimized. The optimal stacking conditions for LVSRP were as follows: hydrodynamic sample injection at 41.4 kPa (60 s), then injection of a short plug of buffer at 13.8 kPa × 15 s, stacking of sample at a voltage of 10 kV with a back pressure of 34.5 kPa, and separation in phosphate buffer (pH 5.63, 100 mmol/L) at a voltage of 15 kV. The optimal stacking conditions for LVSEP were as follows: hydrodynamic sample injection at 48.3 kPa (60 s), and stacking and separation in a buffer of 100 mmol/L phosphate-0.5 mmol/L cetyltrimethylammonium bromide (CTAB) (pH 5.31) at a voltage of 20 kV. About 40-60 fold improvement of sensitivity was achieved without loss of separation efficiency by LVSRP and LVSEP, compared with the normal CZE method. Under the optimum conditions, excellent linear responses were obtained in the concentration range of 0.080-5.0 μmol/L with the correlation coefficient ranging from 0.9962 to 0.9999. The relative standard deviations (RSDs) of peak height were 2.4%-2.7% and 0.6%-4.0% for LVSRP and LVSEP, respectively. The concentration limits of detection (defined as the signal-to-noise ratio of 3) of carnosine, anserine, and homocarnosine obtained for LVSRP and LVSEP were 41-58 nmol/L and 35-43 nmol/L, respectively. © 2007 Chinese Chemical Society and Dalian Institute of Chemical Physics, Chinese Academy of Sciences.
Keyword ：
capillary electrophoresis (CE); carnosine-related peptides; online sample stacking capillary electrophoresis (CE); carnosine-related peptides; online sample stacking
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | HUANG, Y. , DUAN, J. , ZHANG, J. et al. Online Sample Stacking for Determination of Carnosine-Related Peptides by Capillary Electrophoresis [J]. | Chinese Journal of Chromatography , 2007 , 25 (3) : 326-332 . |
| MLA | HUANG, Y. et al. "Online Sample Stacking for Determination of Carnosine-Related Peptides by Capillary Electrophoresis" . | Chinese Journal of Chromatography 25 . 3 (2007) : 326-332 . |
| APA | HUANG, Y. , DUAN, J. , ZHANG, J. , CHEN, G. . Online Sample Stacking for Determination of Carnosine-Related Peptides by Capillary Electrophoresis . | Chinese Journal of Chromatography , 2007 , 25 (3) , 326-332 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
以荧光素异硫氰酸盐柱前衍生了3种激肽类生物活性肽,较为详细地研究了衍生反应条件以及毛细管电泳的分离条件,建立了毛细管电泳激光诱导荧光法分离检测舒缓激肽、胰激肽、神经激肽A的方法。
Keyword ：
异硫氰酸盐 异硫氰酸盐 毛细管电泳 毛细管电泳 生物活性肽 生物活性肽 神经激肽A 神经激肽A 胰激肽 胰激肽 舒缓激肽 舒缓激肽 荧光检测 荧光检测 荧光素 荧光素
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | 陈颖 , 段建平 , 徐两军 et al. 激肽类生物活性肽的毛细管电泳激光诱导荧光检测 [C] //中国化学会第十四届有机分析及生物分析学术研讨会论文集 . 2007 : 199 . |
| MLA | 陈颖 et al. "激肽类生物活性肽的毛细管电泳激光诱导荧光检测" 中国化学会第十四届有机分析及生物分析学术研讨会论文集 . (2007) : 199 . |
| APA | 陈颖 , 段建平 , 徐两军 , 陈红青 , 陈国南 . 激肽类生物活性肽的毛细管电泳激光诱导荧光检测 中国化学会第十四届有机分析及生物分析学术研讨会论文集 . (2007) : 199 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
采用毛细管电泳安培检测法对厚朴酚及和厚朴酚的分离测定进行了详细研究.工作电极为0.3 mm碳圆盘电极,30 mmol/L、pH 10.0的硼砂-氢氧化钠为运行缓冲液,分离电压21 kV,检测电位0.95 V.在最优化实验条件下,两待测物在6 min以内完全基线分离.厚朴酚及和厚朴酚的线性范围分别为0.2～50 μg/mL和0.4～60 μg/mL,检测限分别为0.06和0.2 μg/mL.应用于中药厚朴以及中成药霍香正气水和保济丸中厚朴酚及和厚朴酚的检测.
Keyword ：
厚朴酚 厚朴酚 和厚朴酚 和厚朴酚 安培检测 安培检测 毛细管电泳 毛细管电泳
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | 陈颖 , 段建平 , 陈红青 et al. 毛细管电泳安培检测法同时测定厚朴酚与和厚朴酚 [J]. | 福州大学学报（自然科学版） , 2007 , 35 (4) : 612-615 . |
| MLA | 陈颖 et al. "毛细管电泳安培检测法同时测定厚朴酚与和厚朴酚" . | 福州大学学报（自然科学版） 35 . 4 (2007) : 612-615 . |
| APA | 陈颖 , 段建平 , 陈红青 , 陈国南 . 毛细管电泳安培检测法同时测定厚朴酚与和厚朴酚 . | 福州大学学报（自然科学版） , 2007 , 35 (4) , 612-615 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
建立了毛细管区带电泳(CZE)在线富集3种肌肽类活性肽(肌肽、鹅肌肽和高肌肽)的两种简便有效的方法.一种是大体积进样反向压力排除基体富集(LVSRP)技术,即通过流体动力学进样,在不改变电源极性的条件下,利用反向压力排除样品基体,电堆积富集后进行CZE分离;另一种是大体积进样电渗流排除基体富集(LVSEP)技术,即通过流体动力学进样,于运行缓冲液中加入溴化十六烷基三甲基铵(CTAB)动态修饰毛细管表面,通过电渗流排除样品基体,改变电源极性后进行CZE分离.与常规CZE相比,LVSRP技术和LVSEP技术使检测灵敏度提高了40～60倍.对影响两种富集过程的一些因素进行了研究,在最优富集条件下考察本方法的线性范围为0.080～5.0μmol/L.对3种生物活性肽的检测限(S/N=3)分别为LVSRP 41～58nmol/L,LVSEP 35～43 nmol/L.
Keyword ：
在线富集 在线富集 毛细管电泳 毛细管电泳 肌肽类活性肽 肌肽类活性肽
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | 黄颖 , 段建平 , 张建华 et al. 肌肽类生物活性肽的毛细管电泳在线富集技术 [J]. | 色谱 , 2007 , 25 (3) : 326-331 . |
| MLA | 黄颖 et al. "肌肽类生物活性肽的毛细管电泳在线富集技术" . | 色谱 25 . 3 (2007) : 326-331 . |
| APA | 黄颖 , 段建平 , 张建华 , 陈国南 . 肌肽类生物活性肽的毛细管电泳在线富集技术 . | 色谱 , 2007 , 25 (3) , 326-331 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
通过将1,2-二肉豆蔻酰磷酯酰胆碱和胆固醇制成磷脂双分子层膜,并涂抹在毛细管的内壁上,形成半永久性膜,可以抑制蛋白质在管壁内的吸附.在涂有磷脂双分子层的毛细管中分别对溶菌酶、细胞色素C、核糖核酸A以及α-糜蛋白酶这四种碱性蛋白质的分离行为进行了考察,采用区带毛细管电泳-紫外检测方法(CZE-UV),在最优的分离测定条件下,即30 mmol/L Tris-HCl缓冲液(pH7.0),检测波长为200 nm,分离电压20 kV,进样时间9 s/0.5psi,四种碱性蛋白质能够在20 min内达到基线分离.
Keyword ：
分离 分离 毛细管区带电泳(CZE) 毛细管区带电泳(CZE) 涂层 涂层 碱性蛋白质 碱性蛋白质 磷脂双分子层 磷脂双分子层
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | 魏伟平 , 吴晓苹 , 姜晖 et al. 涂层法在毛细管电泳分离蛋白质中的应用 [C] //南昌大学学报(理科版) . 2006 : 636-637 . |
| MLA | 魏伟平 et al. "涂层法在毛细管电泳分离蛋白质中的应用" 南昌大学学报(理科版) . (2006) : 636-637 . |
| APA | 魏伟平 , 吴晓苹 , 姜晖 , 段建平 , 陈红青 , 徐两军 et al. 涂层法在毛细管电泳分离蛋白质中的应用 南昌大学学报(理科版) . (2006) : 636-637 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
用毛细管电泳-紫外检测法同时测定了四种黄酮类化合物(山奈酚、芦丁、金丝桃甙和槲皮素),研究了各种条件的影响,得到了优化的实验条件.采用无涂层毛细管(75 μm×58 cm,有效长度48.8 cm),以pH=8.30的5 mmol/L的Na2B4O7-10 mmol/L的NaH2PO4缓冲溶液为运行液,进样压力0.5 psi,进样时间10 s,检测波长为254 nm,当分离电压为20 kV时,山奈酚、芦丁、金丝桃甙和槲皮素在6 min内得到了良好的分离.山奈酚、芦丁、金丝桃甙和槲皮素的线性范围分别为0.50～20.0 μg/mL,1.00～50.0 μg/mL,0.80～50.0 μg/mL和1.00～50.0μg/mL,检测下限分别为0.07 μg/mL,0.08 μg/mL和0.23 μg/mL和0.44 μμg/mL.该方法应用于益母草中黄酮类化合物的测定,结果基本满意.
Keyword ：
毛细管电泳 毛细管电泳 益母草 益母草 黄酮类化合物 黄酮类化合物
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | 徐两军 , 林舒 , 段建平 et al. 毛细管电泳-紫外检测法测定益母草中的黄酮类化合物 [C] //南昌大学学报(理科版) . 2006 : 648-649 . |
| MLA | 徐两军 et al. "毛细管电泳-紫外检测法测定益母草中的黄酮类化合物" 南昌大学学报(理科版) . (2006) : 648-649 . |
| APA | 徐两军 , 林舒 , 段建平 , 陈红青 , 许雪琴 . 毛细管电泳-紫外检测法测定益母草中的黄酮类化合物 南昌大学学报(理科版) . (2006) : 648-649 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
蜕皮激素包括羟基蜕皮甾酮(ecdysterone,EDS)和牛膝甾酮.其中EDS有促进蛋白质合成、抑制药物引起的血糖升高、改善肝功和降低血浆胆固醇的作用.目前已有用HPLC测定蜕皮甾酮的报道,但我们选择的这3种中药材中的蜕皮甾酮含量的测定却很少有报道.本法以C18柱为分离柱,甲醇和水作流动相,分别测定了中药材紫背金盘、网果和金疮小草中20-羟基蜕皮甾酮的含量,方法可靠,适合多种中药材中蜕皮甾酮的测定。
Keyword ：
中药材 中药材 含量测定 含量测定 牛膝甾酮 牛膝甾酮 紫背金盘 紫背金盘 羟基蜕皮甾酮 羟基蜕皮甾酮 蜕皮甾酮 蜕皮甾酮 金疮小草 金疮小草 高效液相色谱法 高效液相色谱法
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | 吴红京 , 段建平 . 高效液相色谱法测定中药材中蜕皮甾酮的含量 [C] //全国生物医药色谱学术交流会论文集 . 2006 : 191-192 . |
| MLA | 吴红京 et al. "高效液相色谱法测定中药材中蜕皮甾酮的含量" 全国生物医药色谱学术交流会论文集 . (2006) : 191-192 . |
| APA | 吴红京 , 段建平 . 高效液相色谱法测定中药材中蜕皮甾酮的含量 全国生物医药色谱学术交流会论文集 . (2006) : 191-192 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
A capillary electrophoresis method, using field-amplified sample injection (FASI), was developed for separation and determination of some beta(2)-agonists, such as cimaterol, clenbuterol and salbutamol. The optimum conditions for this system had been investigated in detail. The precision of the migration time, peak height and accuracy were determined in both intra-day (n = 5) and inter-day (n = 15) assays. Under the optimum conditions, the detection limits (defined as S/N = 3) of this method were found to be lower than 2.0 ng/mL for all of these three beta(2)-agonists, which were much lower than that of the conventional electro-migration injection method, the enhancement factors were greatly improved to be 30-40-fold. Such lower detection limit lets this method to be suitable for determination of above-mentioned beta(2)-agonists in the urine sample. The mean recoveries in urine were higher than 96.2%, 95.6% and 95.3% for cimaterol, clenbuterol and salbutamol, respectively, with relative standard deviations lower than 3.5%. (c) 2006 Elsevier B.V. All rights reserved.
Keyword ：
beta(2)-agonists beta(2)-agonists cimaterol cimaterol clenbuterol clenbuterol field-amplified sample injection field-amplified sample injection on-line sample stacking on-line sample stacking salbutamol salbutamol
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Shi, Yanfang , Huang, Ying , Duan, Jianping et al. Field-amplified on-line sample stacking for separation and determination of cimaterol, clenbuterol and salbutamol using capillary electrophoresis [J]. | JOURNAL OF CHROMATOGRAPHY A , 2006 , 1125 (1) : 124-128 . |
| MLA | Shi, Yanfang et al. "Field-amplified on-line sample stacking for separation and determination of cimaterol, clenbuterol and salbutamol using capillary electrophoresis" . | JOURNAL OF CHROMATOGRAPHY A 1125 . 1 (2006) : 124-128 . |
| APA | Shi, Yanfang , Huang, Ying , Duan, Jianping , Chen, Hongqing , Chen, Guonan . Field-amplified on-line sample stacking for separation and determination of cimaterol, clenbuterol and salbutamol using capillary electrophoresis . | JOURNAL OF CHROMATOGRAPHY A , 2006 , 1125 (1) , 124-128 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
A new flow injection analysis (FIA) system equipped with an electrochemiluminescent (ECL) detector has been developed and applied for the ECL detection of 2-thiouracil. The FIA-ECL system used a specially designed flow-through ECL thin-layer cell to reduce the dead volume, the IR drop across the cell, and the probability of accumulation of gas bubbles in the cell. It was thus envisioned to improve the detection limit of the FIA-ECL method. After being established, the new FIA-ECL system was used to investigate the ECL response of 2-thiouracil in the presence of the ECL of Ru(bpy)(3)(2+). It was found that 2-thiouracil could enhance the ECL of Ru(bpy)(3)(2+) over a wide pH range (pH 4.0-12.0). A highly sensitive method for detection of 2-thiouracil in biological samples was developed by the new FIA-ECL system after optimizing several experimental conditions, such as the applied potential of the working electrode, the pH value of the aqueous solution, the flow rate of carrier solution, and the concentration of Ru(bpy)(3)(2+).
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Chi, YW , Duan, JP , Lin, SD et al. Flow injection analysis system equipped with a newly designed electrochemiluminescent detector and its application for detection of 2-thiouracil [J]. | ANALYTICAL CHEMISTRY , 2006 , 78 (5) : 1568-1573 . |
| MLA | Chi, YW et al. "Flow injection analysis system equipped with a newly designed electrochemiluminescent detector and its application for detection of 2-thiouracil" . | ANALYTICAL CHEMISTRY 78 . 5 (2006) : 1568-1573 . |
| APA | Chi, YW , Duan, JP , Lin, SD , Chen, GN . Flow injection analysis system equipped with a newly designed electrochemiluminescent detector and its application for detection of 2-thiouracil . | ANALYTICAL CHEMISTRY , 2006 , 78 (5) , 1568-1573 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
A method of capillary electrophoresis with wall-jet amperometric detection (AD) has been developed for separation and determination of L-tyrosine (Tyr) and its metabolites. such as Tyramine (TA), p-hydroxyphenylpyruvic (pHPP), homogentisic acid (HGA) and sonic dipeptides containing Tyr. such as Tyr-Gly-Gly (YGG), Tyr-Arg (YR) and Tyr-D-Arg (Y-D-R). A carbon disk electrode was used as the working electrode and the optimal detection potential was 1.00 V (versus Ag/AgCl). At 18 kV of applied voltage, the seven compounds were completely separated within 20 min in 110 x 10(-3) mol/L Na2HPO4-NaH2PO4 buffer (pH 7.10) containing 3 x 10(-3) mol/L beta-cyclodextrin (beta-CD). Good linear relationship was obtained for all analytes and the detection limits of seven analytes were in the range of 0.95-4.25 ng/mL. The proposed method has been applied to examine the metabolic process Of L-tyrosine in rabbit's urine. (c) 2006 Elsevier B.V. All rights reserved.
Keyword ：
amperometric detection amperometric detection beta-cyclodextrin beta-cyclodextrin capillary electrophoresis capillary electrophoresis L-tyrosine L-tyrosine pharmacokinetics pharmacokinetics
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Huang, Ying , Jiang, Xiuyan , Wang, Wei et al. Separation and determination of L-tyrosine and its metabolites by capillary zone electrophoresis with a wall-jet amperometric detection [J]. | TALANTA , 2006 , 70 (5) : 1157-1163 . |
| MLA | Huang, Ying et al. "Separation and determination of L-tyrosine and its metabolites by capillary zone electrophoresis with a wall-jet amperometric detection" . | TALANTA 70 . 5 (2006) : 1157-1163 . |
| APA | Huang, Ying , Jiang, Xiuyan , Wang, Wei , Duan, Jianping , Chen, Guonan . Separation and determination of L-tyrosine and its metabolites by capillary zone electrophoresis with a wall-jet amperometric detection . | TALANTA , 2006 , 70 (5) , 1157-1163 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Export
| Results： |
Selected to |
| Format： |
