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学者姓名：王航
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目的:观察可溶性糖蛋白130(sgp130)对糖尿病(DM)小鼠视网膜p-STAT3及VEGF-A表达的影响,探讨sgp130防治糖尿病视网膜病变(DR)炎性损害的可能性.方法:小鼠45只被随机分为正常组、DM组和sgp130组.采用链脲佐菌素对DM组和sgp130组进行DM造模.正常组、DM组不做特殊干预,sgp130组在第1、5wk被给予1.5mg/mL sgp1302μL进行玻璃体腔注射治疗.10wk后处死所有小鼠,检查视网膜组织IL-6、p-STAT3、VEGF-A等蛋白的表达.结果:在第10wk时,DM组视网膜IL-6、p-STAT3、VEGF-A表达较正常组升高(均P<0.01).sgp130组p-STAT3、VEGF-A表达水平较DM组低(均P<0.01).结论:sgp130可以选择性拮抗IL-6反式信号传导通路,下调下游炎性因子VEGF-A的表达,可以用于干预DM引起的IL-6相关性视网膜炎性损害.
Keyword ：
p-STAT3 p-STAT3 可溶性糖蛋白130(sgp130) 可溶性糖蛋白130(sgp130) 炎症 炎症 白介素6 白介素6 糖尿病视网膜病变 糖尿病视网膜病变 血管内皮生长因子A(VEGF-A) 血管内皮生长因子A(VEGF-A)
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| GB/T 7714 | 刘光辉 , 史常旋 , 洪雅军 et al. sgp130对糖尿病小鼠视网膜p-STAT3及VEGF-A表达的影响 [J]. | 国际眼科杂志 , 2023 , 23 (3) : 375-378 . |
| MLA | 刘光辉 et al. "sgp130对糖尿病小鼠视网膜p-STAT3及VEGF-A表达的影响" . | 国际眼科杂志 23 . 3 (2023) : 375-378 . |
| APA | 刘光辉 , 史常旋 , 洪雅军 , 郑永征 , 王航 , 孟春 . sgp130对糖尿病小鼠视网膜p-STAT3及VEGF-A表达的影响 . | 国际眼科杂志 , 2023 , 23 (3) , 375-378 . |
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A real-time electric nose (E-nose) with a metal oxide sensor (MOS) array was developed to monitor 5 highly flammable liquids (ethanol, tetrahydrofuran, turpentine, lacquer thinner, and gasoline) in this work. We found that temperature had a significant impact on the test results and temperature control could efficiently improve the performance of our E-nose. The results of our qualitative analysis showed that principal component analysis (PCA) could not efficiently distinguish these samples compared to a back-propagation artificial neural network (BP-ANN) which had a 100% accuracy rate on the test samples. Quantitative analysis was performed by regression analysis and the average errors were 9.1%-18.4%. In addition, through anti-interference training, the E-nose could filter out the potential false alarm caused by mosquito repellent, perfume and hair jelly.
Keyword ：
artificial neural network artificial neural network back propagation back propagation electronic nose electronic nose flammable liquids flammable liquids qualitative and quantitative analysis qualitative and quantitative analysis
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| GB/T 7714 | Wu, Zhiyuan , Wang, Hang , Wang, Xiping et al. Development of Electronic Nose for Qualitative and Quantitative Monitoring of Volatile Flammable Liquids [J]. | SENSORS , 2020 , 20 (7) . |
| MLA | Wu, Zhiyuan et al. "Development of Electronic Nose for Qualitative and Quantitative Monitoring of Volatile Flammable Liquids" . | SENSORS 20 . 7 (2020) . |
| APA | Wu, Zhiyuan , Wang, Hang , Wang, Xiping , Zheng, Hunlong , Chen, Zhiming , Meng, Chun . Development of Electronic Nose for Qualitative and Quantitative Monitoring of Volatile Flammable Liquids . | SENSORS , 2020 , 20 (7) . |
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新型冠状病毒(severe acute respiratory syndrome coronavirus-2, SARS-CoV-2)是一种可引起人新型冠状病毒肺炎(novel coronavirus pneumonia, NCP;亦称为COVID-19)的新发呼吸道病原体,与中东呼吸综合征冠状病毒(Middle East respiratory syndrome coronavirus, MERS-CoV)和严重急性呼吸综合征冠状病毒(severe acute respiratory syndrome coronavirus, SARS-CoV)同属β-冠状病毒,其受体与SARS-CoV的受...
Keyword ：
新型冠状病毒 新型冠状病毒 疫苗 疫苗 肺炎 肺炎 血管紧张素转化酶2 血管紧张素转化酶2
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| GB/T 7714 | 梁育玮 , 赖玲玲 , 王航 . 新型冠状病毒致病机理及其疫苗的研发进展 [J]. | 微生物学免疫学进展 , 2020 , 48 (04) : 68-73 . |
| MLA | 梁育玮 et al. "新型冠状病毒致病机理及其疫苗的研发进展" . | 微生物学免疫学进展 48 . 04 (2020) : 68-73 . |
| APA | 梁育玮 , 赖玲玲 , 王航 . 新型冠状病毒致病机理及其疫苗的研发进展 . | 微生物学免疫学进展 , 2020 , 48 (04) , 68-73 . |
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为开展半滑舌鳎(Cynoglossus semilaevis)免疫学研究提供细胞平台, 利用密度梯度离心法分离半滑舌鳎外周血淋巴细胞, 采用短期细胞培养法分离悬浮淋巴细胞, 悬浮淋巴细胞在含有0.3 μg/mL的PHA的DMEM完全培养基, 于24℃条件下可连续培养3—4d左右, 采用自制的尼龙毛柱可将悬浮淋巴细胞中的非黏附淋巴细胞和黏附淋巴细胞成功分离; 利用流式细胞仪结合特异抗体检测对非黏附淋巴细胞和黏附淋巴细胞进行鉴定,结果表明, 非黏附细胞与鼠抗人FTIC-CD3单克隆抗体特异结合, 为T样淋巴细胞; 黏附细胞和鼠抗人FTIC-CD19单抗特异结合,为B样淋巴细胞.T细胞表面抗原受体TCRβ基因可特异性的在非黏膜细胞中表达,而在黏附细胞中不表达, 证明分离获得的非黏膜细胞为T淋巴细胞, 采用qRT-PCR (Quantitative Real-Time PCR)方法检测TCRβ基因表达, 结果表明, TCRβ基因在半滑舌鳎肝、脾、头肾、后肾、小肠、胃、血液、鳃、皮肤、肌肉、心脏、脑、卵巢组织中均有表达, 其中在肠、胃、脾、头肾中表达量较高; 鳗弧菌感染后TCRβ基因在肝、脾、鳃中呈现明显的上调表达,且表达峰值出现在感染后72—96h,表明TCRβ基因在获得性免疫应答中起重要作用.
Keyword ：
B淋巴细胞 B淋巴细胞 TCRβ基因 TCRβ基因 T淋巴细胞 T淋巴细胞 免疫应答 免疫应答 半滑舌鳎 半滑舌鳎 外周血 外周血
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| GB/T 7714 | 汪林庆 , 王航 , 陈亚东 et al. 半滑舌鳎外周血T淋巴细胞的分离、鉴定及TCRβ基因免疫应答分析 [J]. | 水生生物学报 , 2018 , 42 (3) : 542-549 . |
| MLA | 汪林庆 et al. "半滑舌鳎外周血T淋巴细胞的分离、鉴定及TCRβ基因免疫应答分析" . | 水生生物学报 42 . 3 (2018) : 542-549 . |
| APA | 汪林庆 , 王航 , 陈亚东 , 杨光 , 白莉 , 孙璐明 et al. 半滑舌鳎外周血T淋巴细胞的分离、鉴定及TCRβ基因免疫应答分析 . | 水生生物学报 , 2018 , 42 (3) , 542-549 . |
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本发明公开了阵列式加样器，其包括若干个加样针、若干个储液模块、若干个输送驱动装置和气源，若干个储液模块与若干个加样针一一对应且连接，若干个输送驱动装置的一端均与气源连接，若干个输送驱动装置的另一端与若干个储液模块一一对应且连接并用于将储液模块中的试剂推送至加样针进行输出加样，其实现了不同试剂使用不同的试剂针进行加样，免去了试剂针清洗的过程，减少了加样所需时间并简化了运动结构，从而可以使每次加样所需时间减少，保证了在多种不同第一抗体试剂滴加过程中不同切片接触试剂时间的差距大大减小，保证了染色结果的一致性与可重复性，在仪器运行过程中所存储的试剂与空气的接触面积极小，保证试剂的安全性和纯净性。
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| GB/T 7714 | 孟春 , 钟乐 , 王航 . 阵列式加样器 : CN201810023012.4[P]. | 2018/1/10 . |
| MLA | 孟春 et al. "阵列式加样器" : CN201810023012.4. | 2018/1/10 . |
| APA | 孟春 , 钟乐 , 王航 . 阵列式加样器 : CN201810023012.4. | 2018/1/10 . |
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A limited number of xenobiotic-induced inflammatory episodes occur in the human liver and small intestine under normal physiological conditions, although many xenobiotic agents are metabolized in these organs every day. In this study, we found that rifampicin-activated pregnane X receptor (PXR) plays an important role in the suppression of lipopolysaccharide-activated nuclear factor kappa B (NF-kappa B) activity by increasing the expression of the inhibitor of kappa B alpha (I kappa B alpha) in HepG2 cells. Rifampicin did not increase the expression of I kappa B alpha in PXR knockdown HepG2 cells. Furthermore, we found that the activation of PXR could inhibit the lipopolysaccharide-stimulated nuclear translocation of NF-kappa B p50/p65 using electrophoretic mobility shift assay, immunoprecipitation assays, and microscopy. High levels of I kappa B alpha directly interacted with NF-kappa B and prevented its nuclear translocation, thus inhibiting its binding to consensus DNA sequences in nuclei. Xenobiotic-activated tissue-specific PXR might exert anti-inflammatory actions by antagonizing the xenobiotic-induced transcriptional activity of NF-kappa B in the liver and small intestine. The results also showed that activation of PXR arrested the HepG2 cell cycle in the G(0)/G(1) phase and exhibited cancer prevention potential by inhibiting inflammatory reactions in cells.
Keyword ：
Anti-inflammation Anti-inflammation Cancer prevention Cancer prevention Inhibitor of kappa B alpha Inhibitor of kappa B alpha Pregnane X receptor Pregnane X receptor
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| GB/T 7714 | Ye, Nanhui , Wang, Hang , Li, Qiaoling et al. Activation of PXR inhibits LPS-induced NF-kappa B activation by increasing I kappa B alpha expression in HepG2 cells [J]. | MOLECULAR & CELLULAR TOXICOLOGY , 2018 , 14 (1) : 93-104 . |
| MLA | Ye, Nanhui et al. "Activation of PXR inhibits LPS-induced NF-kappa B activation by increasing I kappa B alpha expression in HepG2 cells" . | MOLECULAR & CELLULAR TOXICOLOGY 14 . 1 (2018) : 93-104 . |
| APA | Ye, Nanhui , Wang, Hang , Li, Qiaoling , Lin, Chaotong , Feng, Huahua , Lin, Suying et al. Activation of PXR inhibits LPS-induced NF-kappa B activation by increasing I kappa B alpha expression in HepG2 cells . | MOLECULAR & CELLULAR TOXICOLOGY , 2018 , 14 (1) , 93-104 . |
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The Escherichia coli aspartate aminotransferase gene was introduced into a high 2-phenylethanol (2-PEA) producing Saccharomyces cerevisiae YS58, and the recombinant strain of S. cerevisiae was utilized for the co-production of 2-PEA and L-homophenylalanine (L-HPA) via a fermentation process. The L-HPA productivity of the recombinant S. cerevisiae improved 78.9% in comparison to the wild-type S. cerevisiae. High yields of 43.7 mM L-HPA and 32.4 mM 2-PEA were achieved. As a result, the coupling of the biosynthesis process for these two products in the recombinant strain led to a more complete and efficient utilization of the substrate, L-phenylalanine. (C) 2016, The Society for Biotechnology, Japan. All rights reserved.
Keyword ：
2-Phenylethanol 2-Phenylethanol Aspartate aminotransferase Aspartate aminotransferase Cosynthesis Cosynthesis L-Homophenylalanine L-Homophenylalanine Saccharomyces cerevisiae Saccharomyces cerevisiae
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| GB/T 7714 | Luo, Chunhua , Lin, Qingmei , Lin, Suying et al. Cosynthesis of L-homophenylalanine and 2-phenylethanol by recombinant Saccharomyces cerevisiae expressing aspartate aminotransferase from Escherichia coli BL21(DE3) [J]. | JOURNAL OF BIOSCIENCE AND BIOENGINEERING , 2017 , 123 (1) : 1-7 . |
| MLA | Luo, Chunhua et al. "Cosynthesis of L-homophenylalanine and 2-phenylethanol by recombinant Saccharomyces cerevisiae expressing aspartate aminotransferase from Escherichia coli BL21(DE3)" . | JOURNAL OF BIOSCIENCE AND BIOENGINEERING 123 . 1 (2017) : 1-7 . |
| APA | Luo, Chunhua , Lin, Qingmei , Lin, Suying , Meng, Chun , Wang, Hang . Cosynthesis of L-homophenylalanine and 2-phenylethanol by recombinant Saccharomyces cerevisiae expressing aspartate aminotransferase from Escherichia coli BL21(DE3) . | JOURNAL OF BIOSCIENCE AND BIOENGINEERING , 2017 , 123 (1) , 1-7 . |
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The pregnane X receptor (PXR), a liver and intestine specific receptor has been reported to be related with the repression of inflammation as well as activation of cytochronnosome P450 3A (CYP3A) expression. We examined the effect of PXR on tetrachloromethane (CCl4)-induced mouse liver inflammation in this work. Ginkgolide A, one main component of Ginkgo biloba extracts (GBE), activated PXR and enhanced PXR expression level, displayed both significant therapeutic effect and preventive effect against CCl4-induced mouse hepatitis. siRNA-mediated decrease of PXR expression significantly reduced the efficacy of Ginkgolide A in treating CCl4-induced inflammation in mice. Flavonoids, another important components of GBE, were shown anti-inflammatory effect in a different way from Ginkgolide A which might be independent on PXR because flavonoids significantly inhibited CYP3A11 activities in mice. The results indicated that anti-inflammatory effect of PXR might be mediated by enhancing transcription level of I kappa B alpha through binding of I kappa B alpha. Inhibition of NF-kappa B activity by NF-kappa B-specific suppressor I kappa B alpha is one of the potential mechanisms of Ginkgolide A against CCl4-induced liver inflammation.
Keyword ：
Ginkgolide A Ginkgolide A I kappa B alpha expression I kappa B alpha expression Liver inflammation Liver inflammation Pregnane X receptor Pregnane X receptor PXR knock-down PXR knock-down
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| GB/T 7714 | Ye, Nanhui , Wang, Hang , Hong, Jing et al. PXR Mediated Protection against Liver Inflammation by Ginkgolide A in Tetrachloromethane Treated Mice [J]. | BIOMOLECULES & THERAPEUTICS , 2016 , 24 (1) : 40-48 . |
| MLA | Ye, Nanhui et al. "PXR Mediated Protection against Liver Inflammation by Ginkgolide A in Tetrachloromethane Treated Mice" . | BIOMOLECULES & THERAPEUTICS 24 . 1 (2016) : 40-48 . |
| APA | Ye, Nanhui , Wang, Hang , Hong, Jing , Zhang, Tao , Lin, Chaotong , Meng, Chun . PXR Mediated Protection against Liver Inflammation by Ginkgolide A in Tetrachloromethane Treated Mice . | BIOMOLECULES & THERAPEUTICS , 2016 , 24 (1) , 40-48 . |
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Background The presence of cancer stem cells (CSCs) is currently regarded as one of the main culprits of tumor formation and therapy failure. It is known that chronic inflammation is associated with CSCs, but it is not clear yet how inflammation affects the development of CSCs. In the present study we aimed to examine the relationship between cancer cell stimulation mediated by immune cells and the acquisition of a CSC-like phenotype. Methods Cancer cells derived from single hepatocarcinoma HepG2 cells were treated with mouse splenic B cells (MSBCs) and mouse peritoneal macrophage cells (MPMCs), respectively. The stem cell-like characteristics of the resulting HepG2 cells (MSBC-HepG2 and MPMC-HepG2) were evaluated using different assays, including biomarker assays, in vitro tumoroid and colony forming assays, in vivo tumor forming assays and signal transduction pathway activation assays. Results Various stemness characteristics of HepG2 cells, including self-renewal, proliferation, chemoresistance and tumorigenicity were evaluated. The expression levels of stemness-related genes and its encoded proteins in the MSBC-HepG2 and MPMC-HepG2 cells were assessed using RT-PCR and FACS analyses. We found that MSBC-HepG2 and MPMC-HepG2 cells possess hepatic CSC properties, including persistent self-renewal, extensive proliferation, drug resistance, high tumorigenic capacity and over-expression of CSC-related genes and proteins (i.e., EpCAM, ALDH, CD133 and CD44), compared to the parental cells. We also found that 1x10(3) MSBC-HepG2 and MPMC-HepG2 cells were able to form tumors in NOD/SCID mice and that the Notch and SHH signaling pathways were highly activated in MSBC-HepG2 cells. Conclusions We conclude that the immune system may have a double-edge effect on cancer development. On one hand, immune cells such as B lymphocytes and macrophages may recognize, attack and eliminate cancer cells, whereas on the other hand, they may promote a subset of cancer cells to acquire stem cell-like characteristics.
Keyword ：
Cancer stem cells Cancer stem cells HepG2 cells HepG2 cells Peritoneal macrophage cells Peritoneal macrophage cells Splenic B cells Splenic B cells
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| GB/T 7714 | Wang, Hang , Yang, Miqing , Lin, Ling et al. HepG2 cells acquire stem cell-like characteristics after immune cell stimulation [J]. | CELLULAR ONCOLOGY , 2016 , 39 (1) : 35-45 . |
| MLA | Wang, Hang et al. "HepG2 cells acquire stem cell-like characteristics after immune cell stimulation" . | CELLULAR ONCOLOGY 39 . 1 (2016) : 35-45 . |
| APA | Wang, Hang , Yang, Miqing , Lin, Ling , Ren, Hongzhen , Lin, Chaotong , Lin, Suling et al. HepG2 cells acquire stem cell-like characteristics after immune cell stimulation . | CELLULAR ONCOLOGY , 2016 , 39 (1) , 35-45 . |
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IL-6 (interleukin 6) plays an important role in the development and growth of hepatocellular carcinoma (HCC) via both classic signaling and trans-signaling pathways. Soluble gp130 (sgp130) is known to be a natural inhibitor of the trans-signaling pathway. In the present study, our goal was to investigate whether recombinant sgp130 could suppress the initiation and progression of HCC in mouse models. Our results demonstrate that sgp130 induced an apoptosis of HepG2 cells and inhibited the clonogenicity of HepG2 in vitro. Moreover, the IL-6 trans-signaling pathway is significantly suppressed by sgp130 as reflected by the decrease in the level of STAT3 phosphorylation and other inflammatory factors both in vitro and in vivo. In the DEN-induced HCC mouse model, intravenous injection of sgp130 attenuated hepatic fibrosis at 16 weeks and reduced the initiation and progression of primary HCC at 36 weeks. Furthermore, our results also demonstrate that intravenous administration of sgp130 significantly suppressed the growth and metastasis of xenograft human HCC in NOD/SCID mice.
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| GB/T 7714 | Hong, Jing , Wang, Hang , Shen, Guoying et al. Recombinant soluble gp130 protein reduces DEN-induced primary hepatocellular carcinoma in mice [J]. | SCIENTIFIC REPORTS , 2016 , 6 . |
| MLA | Hong, Jing et al. "Recombinant soluble gp130 protein reduces DEN-induced primary hepatocellular carcinoma in mice" . | SCIENTIFIC REPORTS 6 (2016) . |
| APA | Hong, Jing , Wang, Hang , Shen, Guoying , Lin, Da , Lin, Yanxue , Ye, Nanhui et al. Recombinant soluble gp130 protein reduces DEN-induced primary hepatocellular carcinoma in mice . | SCIENTIFIC REPORTS , 2016 , 6 . |
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