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学者姓名：林峻
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Purpose: This study aims to investigate the antibiotic resistance and virulence factors of Paenibacillus campinasensis, an understudied opportunistic pathogen, through whole-genome sequencing and resistance genes. The findings will contribute to the development of effective clinical strategies for infection control. Patients and Methods: A 1-year and 3-month-old boy presenting with fever and rash was admitted to the hospital and clinically diagnosed with hand-foot-mouth disease. A blood culture was obtained, and the pathogen was preliminarily identified as belonging to the genus Paenibacillus using MALDI-TOF-MS. Subsequent 16S rDNA PCR product Sanger sequencing confirmed the isolate as Paenibacillus campinasensis. Whole-genome sequencing and bioinformatics analysis were performed to evaluate the antibiotic resistance and virulence factors of the strain. Results: Whole-genome sequencing and bioinformatics analysis identified 18 resistance genes in the Paenibacillus campinasensis strain, spanning multiple antibiotic categories. These findings suggest the potential for multidrug resistance in this bacterium, despite its isolation from blood without associated clinical infection in the patient. Conclusion: The identification of resistance genes in Paenibacillus campinasensis underscores the challenges in clinical treatment and emphasizes the critical role of whole-genome analysis in pathogen detection and characterization. These findings are vital for informing effective clinical strategies to address infections, particularly given the potential for antibiotic resistance to hinder treatment outcomes, even in the absence of direct disease causation by the bacterium.
Keyword ：
antibiotic resistance antibiotic resistance bioinformatics analysis bioinformatics analysis multidrug resistance multidrug resistance virulence factors virulence factors whole-genome sequencing whole-genome sequencing
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| GB/T 7714 | Yu, Xiaoling , Lin, Jun , Jiang, Wenqian et al. Whole-Genome Analysis of Paenibacillus campinasensis from Blood of a Hand-Foot-Mouth Patient [J]. | INFECTION AND DRUG RESISTANCE , 2025 , 18 : 2663-2672 . |
| MLA | Yu, Xiaoling et al. "Whole-Genome Analysis of Paenibacillus campinasensis from Blood of a Hand-Foot-Mouth Patient" . | INFECTION AND DRUG RESISTANCE 18 (2025) : 2663-2672 . |
| APA | Yu, Xiaoling , Lin, Jun , Jiang, Wenqian , Zhu, Tianzhu , Lin, Xiao , Liu, Xiaolong et al. Whole-Genome Analysis of Paenibacillus campinasensis from Blood of a Hand-Foot-Mouth Patient . | INFECTION AND DRUG RESISTANCE , 2025 , 18 , 2663-2672 . |
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Acute respiratory infections, caused by RNA viruses like respiratory syncytial virus, influenza, rhinovirus, and coronavirus, are major global health threats. Real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR) is the gold standard for detecting these viruses but is time-consuming, complex, and requires specialized equipment. There is a need for rapid, convenient, and multi-target detection methods to improve disease prevention and control. This study developed a multi-target immunochromatographic detection method using LbuCas13a protein and "band elimination" test strips for detecting SARS-CoV-2 and influenza virus. The method's performance was evaluated by testing known 5 positive and 4 negative samples for SARS-CoV-2 and comparing results with fluorescent PCR and colloidal gold methods. Detection sensitivity was quantified using digital PCR and qPCR. The immunochromatographic test strips showed 100% concordance with fluorescent PCR and colloidal gold methods in initial clinical SARS-CoV-2 detection. Subsequently, we used dual-target immunochromatographic test strips to detect 9 SARS-CoV-2 positive samples and 9 H3N2 positive samples. However, false negatives were observed in dual-target detection of SARS-CoV-2 and H3N2 samples, likely due to low sample concentration or sample degradation. The method had a minimum detection limit of 381.75 copies/mu L, as determined by digital PCR and qPCR. The developed multi-target immunochromatographic detection method offers a rapid, low-cost, and simple approach for detecting both SARS-CoV-2 and influenza viruses. With high sensitivity, specificity, and reliability, this method holds promise as a practical tool for RNA virus diagnosis and improving public health response to respiratory infections.
Keyword ：
CRISPR-Cas13a CRISPR-Cas13a Immunochromatographic test strip Immunochromatographic test strip Multi-target detection Multi-target detection Respiratory RNA virus Respiratory RNA virus
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| GB/T 7714 | Wang, Tao , Jiang, Wenqian , Huang, Zhiqing et al. Multiplex detection of respiratory RNA viruses without amplification based on CRISPR-Cas13a immunochromatographic test strips [J]. | VIROLOGY JOURNAL , 2025 , 22 (1) . |
| MLA | Wang, Tao et al. "Multiplex detection of respiratory RNA viruses without amplification based on CRISPR-Cas13a immunochromatographic test strips" . | VIROLOGY JOURNAL 22 . 1 (2025) . |
| APA | Wang, Tao , Jiang, Wenqian , Huang, Zhiqing , Yuan, Zhitao , Chen, Zhiwei , Lin, Jun . Multiplex detection of respiratory RNA viruses without amplification based on CRISPR-Cas13a immunochromatographic test strips . | VIROLOGY JOURNAL , 2025 , 22 (1) . |
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Acinetobacter baumannii is a notable opportunistic pathogen responsible for severe hospital-acquired infections, with multidrug-resistant strains posing significant treatment challenges. Phage therapy, which employs bacteriophages as natural bacterial antagonists, has gained renewed attention as a promising solution to combat antibiotic-resistant infections. In this study, we isolated and characterized a novel virulent phage, vB_AbaS_qsb1, which specifically lyses A.baumannii. Phylogenetic and genomic analyses indicate that vB_AbaS_qsb1 is the founding member of a previously unreported genus, which we propose to name Acinibactriovirus, with Acinibactriovirus lysinus as the type species. vB_AbaS_qsb1 demonstrated robust stability across diverse temperature and pH ranges, a short latent period, and no known virulence or antibiotic resistance genes within its 54,713 bp dsDNA genome. Safety assessments showed that high-dose vB_AbaS_qsb1 induced no adverse effects in mice, with histopathology confirming its safety profile. Therapeutic experiments further indicated that vB_AbaS_qsb1 provided at least 50% protection against A.baumannii-induced pneumonia, significantly reducing bacterial loads and inflammation markers, while maintaining high phage titers in lung tissue.This study introduces vB_AbaS_qsb1 as a promising candidate for phage therapy against A.baumannii, offering both innovative insights and a valuable framework for future isolation, genomic characterization, and efficacy evaluation of phages targeting antibiotic-resistant bacteria.
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| GB/T 7714 | Wang, Jiawang , Hu, Huan , Wang, Qingqing et al. A novel genus of virulent phage targeting Acinetobacter baumannii: Efficacy and safety in a murine model of pulmonary infection [J]. | PLOS PATHOGENS , 2025 , 21 (6) . |
| MLA | Wang, Jiawang et al. "A novel genus of virulent phage targeting Acinetobacter baumannii: Efficacy and safety in a murine model of pulmonary infection" . | PLOS PATHOGENS 21 . 6 (2025) . |
| APA | Wang, Jiawang , Hu, Huan , Wang, Qingqing , Zhu, Tianzhu , Ren, Xiaohan , Jiang, Wenqian et al. A novel genus of virulent phage targeting Acinetobacter baumannii: Efficacy and safety in a murine model of pulmonary infection . | PLOS PATHOGENS , 2025 , 21 (6) . |
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Here, the mixed metal nodes MOFs, Pd/MIL-100(FeaCub), were constructed as photocatalysts for hydrogenation of alpha,beta-unsaturated aldehyde (UAL) under visible light. 1 wt% Pd/MIL-100(Fe0.81Cu0.19) can convert a range of UAL to saturated aldehydes (SAL) with a high efficiency (approximate to 100 %) and selectivity (approximate to 98 %). The results of XPS and in situ DRIFTS reveal that UAL can be selectively activated via a coordination of -C--C- on Cu2+ sites, determining the high selectivity of the photocatalytic reaction. The mixed metal nodes and Pd clusters can improve the transformation and separation of photogenerated electrons-holes. EPR result suggests that photogenerated carriers can facilitate the generation of H center dot on Pd/MIL-100(Fe0.81Cu0.19), enhancing the catalytic activity. A possible mechanism is proposed for elucidating the catalytic processes at the molecular level. This work provides a valuable strategy for tailoring the selectivity of photocatalytic hydrogenation via selective coordination activation.
Keyword ：
alpha alpha beta-Unsaturated aldehydes beta-Unsaturated aldehydes Coordination activation Coordination activation Hydrogenation Hydrogenation MIL-100(Fe/Cu) MIL-100(Fe/Cu) Photocatalyst Photocatalyst
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| GB/T 7714 | Wang, Zhiwen , Kang, Yueyue , Shi, Yingzhang et al. Selective coordination activation regulating the selectivity for photocatalytic hydrogenation of α,β-unsaturated aldehyde over Pd/ MIL-100(FeaCub) [J]. | APPLIED CATALYSIS B-ENVIRONMENTAL , 2024 , 340 . |
| MLA | Wang, Zhiwen et al. "Selective coordination activation regulating the selectivity for photocatalytic hydrogenation of α,β-unsaturated aldehyde over Pd/ MIL-100(FeaCub)" . | APPLIED CATALYSIS B-ENVIRONMENTAL 340 (2024) . |
| APA | Wang, Zhiwen , Kang, Yueyue , Shi, Yingzhang , Liu, Cheng , Liu, Yunni , Lin, Jun et al. Selective coordination activation regulating the selectivity for photocatalytic hydrogenation of α,β-unsaturated aldehyde over Pd/ MIL-100(FeaCub) . | APPLIED CATALYSIS B-ENVIRONMENTAL , 2024 , 340 . |
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Trauma is a significant health issue that not only leads to immediate death in many cases but also causes severe complications, such as sepsis, thrombosis, haemorrhage, acute respiratory distress syndrome and traumatic brain injury, among trauma patients. Target protein identification technology is a vital technique in the field of biomedical research, enabling the study of biomolecular interactions, drug discovery and disease treatment. It plays a crucial role in identifying key protein targets associated with specific diseases or biological processes, facilitating further research, drug design and the development of treatment strategies. The application of target protein technology in biomarker detection enables the timely identification of newly emerging infections and complications in trauma patients, facilitating expeditious medical interventions and leading to reduced post-trauma mortality rates and improved patient prognoses. This review provides an overview of the current applications of target protein identification technology in trauma-related complications and provides a brief overview of the current target protein identification technology, with the aim of reducing post-trauma mortality, improving diagnostic efficiency and prognostic outcomes for patients.
Keyword ：
biomarkers biomarkers mass spectrometry mass spectrometry target protein identification target protein identification trauma trauma trauma-related complications trauma-related complications
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| GB/T 7714 | Wei, YiLiu , Ren, Xiaohan , Yuan, Zhitao et al. Trauma diagnostic-related target proteins and their detection techniques [J]. | EXPERT REVIEWS IN MOLECULAR MEDICINE , 2024 , 26 . |
| MLA | Wei, YiLiu et al. "Trauma diagnostic-related target proteins and their detection techniques" . | EXPERT REVIEWS IN MOLECULAR MEDICINE 26 (2024) . |
| APA | Wei, YiLiu , Ren, Xiaohan , Yuan, Zhitao , Hong, Jie , Wang, Tao , Chen, Weizhi et al. Trauma diagnostic-related target proteins and their detection techniques . | EXPERT REVIEWS IN MOLECULAR MEDICINE , 2024 , 26 . |
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Background Nasopharyngeal carcinoma (NPC) treatment is largely based on a 'one-drug-fits-all' strategy in patients with similar pathological characteristics. However, given its biological heterogeneity, patients at the same clinical stage or similar therapies exhibit significant clinical differences. Thus, novel molecular subgroups based on these characteristics may better therapeutic outcomes. Methods Herein, 192 treatment-naive NPC samples with corresponding clinicopathological information were obtained from Fujian Cancer Hospital between January 2015 and January 2018. The gene expression profiles of the samples were obtained by RNA sequencing. Molecular subtypes were identified by consensus clustering. External NPC cohorts were used as the validation sets. Results Patients with NPC were classified into immune, metabolic, and proliferative molecular subtypes with distinct clinical features. Additionally, this classification was repeatable and predictable as validated by the external NPC cohorts. Metabolomics has shown that arachidonic acid metabolites were associated with NPC malignancy. We also identified several key genes in each subtype using a weighted correlation network analysis. Furthermore, a prognostic risk model based on these key genes was developed and was significantly associated with disease-free survival (hazard ratio, 1.11; 95% CI, 1.07-1.16; P < 0.0001), which was further validated by an external NPC cohort (hazard ratio, 7.71; 95% CI, 1.39-42.73; P < 0.0001). Moreover, the 1-, 3-, and 5-year areas under the curve were 0.84 (95% CI, 0.74-0.94), 0.81 (95% CI, 0.73-0.89), and 0.82 (95% CI, 0.73-0.90), respectively, demonstrating a high predictive value. Conclusions Overall, we defined a novel classification of nasopharyngeal carcinoma (immune, metabolism, and proliferation subtypes). Among these subtypes, metabolism and proliferation subtypes were associated with advanced stage and poor prognosis of NPC patients, whereas the immune subtype was linked to early stage and favorable prognosis.
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| GB/T 7714 | Lin, Wanzun , Chen, Xiaochuan , Huang, Zongwei et al. Identification of novel molecular subtypes to improve the classification framework of nasopharyngeal carcinoma [J]. | BRITISH JOURNAL OF CANCER , 2024 , 130 (7) : 1176-1186 . |
| MLA | Lin, Wanzun et al. "Identification of novel molecular subtypes to improve the classification framework of nasopharyngeal carcinoma" . | BRITISH JOURNAL OF CANCER 130 . 7 (2024) : 1176-1186 . |
| APA | Lin, Wanzun , Chen, Xiaochuan , Huang, Zongwei , Ding, Qin , Yang, Hanxuan , Li, Ying et al. Identification of novel molecular subtypes to improve the classification framework of nasopharyngeal carcinoma . | BRITISH JOURNAL OF CANCER , 2024 , 130 (7) , 1176-1186 . |
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Glioma stem cells (GSCs) are thought to be responsible for the initiation and progression of glioblastoma (GBM). GBM presents highly invasive growth with a very high recurrence rate, so it has become a clinical problem to be solved urgently. RNAseq demonstrates that thrombospondin 1 (THBS1) acts not only in the angiogenic core of glioma but also with a high degree of invasiveness and infiltration. Nevertheless, defects in the signaling pathway research lead to a poor prognosis in glioma patients. To investigate the relevant molecular mechanism and signal pathway of glioma stem cell behavior mediated by THBS1, U251 astroglioma cells and GSCs were taken as model cells for in vitro experiments. The biological effects of THBS1 on glioma proliferation, migration, and adhesion were evaluated using Cell Counting Kit-8(CCK8) assays, EdU incorporation assays, migration assays, Transwell assays, Western blotting, and RNAseq. We found that the knockout of the THBS1 gene by CRISPR/Cas9 promoted proliferation and migration in U251 cells and GSCs, as well as influencing cell cycle progression by regulating the TNF/MAPK/NF-kappa B and TGF-beta/Smad signaling pathways. Moreover, U251 cells and GSCs showed different re-sponses to THBS1 knockout, suggesting specific and potential targets for GSCs in signaling pathways mediated by THBS1.
Keyword ：
Glioma stem cells Glioma stem cells MAPK MAPK Proliferation Proliferation ? signaling pathways ? signaling pathways TGF TGF Thrombospondin 1 Thrombospondin 1 TNF TNF TRAF2 TRAF2
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| GB/T 7714 | Chen, Liqun , Fang, Wei , Chen, Weizhi et al. Deciphering the molecular mechanism of the THBS1 gene in the TNF signaling axis in glioma stem cells [J]. | CELLULAR SIGNALLING , 2023 , 106 . |
| MLA | Chen, Liqun et al. "Deciphering the molecular mechanism of the THBS1 gene in the TNF signaling axis in glioma stem cells" . | CELLULAR SIGNALLING 106 (2023) . |
| APA | Chen, Liqun , Fang, Wei , Chen, Weizhi , Wei, Yiliu , Ding, Jinwang , Li, Jiafeng et al. Deciphering the molecular mechanism of the THBS1 gene in the TNF signaling axis in glioma stem cells . | CELLULAR SIGNALLING , 2023 , 106 . |
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本发明公开了一种可规模化生产具有活性的DNA聚合酶的方法。所述方法包括以下步骤：1)将携带有目的基因的菌种划线于带氨苄抗性的LB平板上，挑取单菌落培养后作为种子液；2)配置高密度发酵培养基，加入发酵罐中；3)将种子液转接到步骤2)的发酵罐中进行培养；4)当培养至溶氧下降至30%以下时，开始加入补料培养基，控制溶氧为30％以上；5)当培养至细菌菌体湿重距最大菌体湿重4g/L时，一次性加入IPTG诱导，使IPTG终浓度为1±0.1 mM；6)达到最佳诱导时长后停止发酵，放罐，获取菌体。本发明提高了目标产物的发酵稳定性，并且减少了包涵体的产生，可实现规模化生产DNA聚合酶。
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| GB/T 7714 | 陈鲤群 , 高上 , 袁智涛 et al. 一种可规模化生产具有活性的DNA聚合酶的方法 : CN202210959921.5[P]. | 2022-08-11 00:00:00 . |
| MLA | 陈鲤群 et al. "一种可规模化生产具有活性的DNA聚合酶的方法" : CN202210959921.5. | 2022-08-11 00:00:00 . |
| APA | 陈鲤群 , 高上 , 袁智涛 , 王涛 , 姜文倩 , 林峻 . 一种可规模化生产具有活性的DNA聚合酶的方法 : CN202210959921.5. | 2022-08-11 00:00:00 . |
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本发明公开了一种丝状真菌复制子，所述复制子的核苷酸序列如SEQ ID NO.2所示。所述复制子是以19种真菌基因组为模板构建宏基因组文库，转化黑曲霉孢子筛选得到，来自厚孢根霉内生菌伯克霍尔德氏菌HKI454基因组。本发明的复制子可以在黑曲霉表达系统中发挥复制功能，并且可以提供比AMA1复制子更高的稳定性，为首次在除构巢曲霉以外的真菌中发现的复制子，丰富了复制子元件库，拓展了丝状真菌利用复制子的选择多样性。
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| GB/T 7714 | 林峻 , 燕天鹤 . 一种丝状真菌复制子 : CN202210598346.0[P]. | 2022-05-30 00:00:00 . |
| MLA | 林峻 et al. "一种丝状真菌复制子" : CN202210598346.0. | 2022-05-30 00:00:00 . |
| APA | 林峻 , 燕天鹤 . 一种丝状真菌复制子 : CN202210598346.0. | 2022-05-30 00:00:00 . |
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本实用新型公开一种采样管定位结构及样品管架，包括样品管放置孔，样品管放置孔上端面的外围设有至少三个沿圆周方向间隔设置的导向棱，各导向棱的凸起面为导向坡面，且导向坡面的低端朝向样品管放置孔。本实用新型采用多个导向棱的结构，用于样品管自动定圆心。此结构定圆心简单快速，从根源解决了定圆心问题。
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| GB/T 7714 | 林峻 , 高上 , 袁智涛 . 一种采样管定位结构及样品管架 : CN202320287361.3[P]. | 2023-02-22 00:00:00 . |
| MLA | 林峻 et al. "一种采样管定位结构及样品管架" : CN202320287361.3. | 2023-02-22 00:00:00 . |
| APA | 林峻 , 高上 , 袁智涛 . 一种采样管定位结构及样品管架 : CN202320287361.3. | 2023-02-22 00:00:00 . |
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