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Abstract ：
Chemotherapy is commonly used to treat malignant tumors. However, conventional chemotherapeutic drugs often cannot distinguish between tumor and healthy cells, resulting in adverse effects and reduced therapeutic efficacy. Therefore, zigzag-shaped gear-occlude-guided cymbal-closing (ZGC) DNA nanotechnology was developed based on the mirror-symmetry principle to efficiently construct symmetric DNA polyhedra. This nanotechnology employed simple mixing steps for efficient sequence design and assembly. A targeting aptamer was installed at a user-defined position using an octahedron as a model structure. Chemotherapeutic drug-loaded polyhedral objects were subsequently delivered into tumor cells. Furthermore, anticancer drug-loaded DNA octahedra were intravenously injected into a HeLa tumor-bearing mouse model. Assembly efficiency was almost 100 %, with no residual building blocks identified. Moreover, this nanotechnology required a few DNA oligonucleotides, even for complex polyhedrons. Symmetric DNA polyhedrons retained their structural integrity for 24 h in complex biological environments, guaranteeing prolonged circulation without drug leakage in the bloodstream and promoting efficient accumulation in tumor tissues. In addition, DNA octahedra were cleared relatively slowly from tumor tissues. Similarly, tumor growth was significantly inhibited in vivo, , and a thera- peutic outcome comparable to that of conventional gene-chemo combination therapy was observed. Moreover, no systemic toxicity was detected. These findings indicate the potential application of ZGC DNA nanotechnology in precision medicine.
Keyword ：
DNA nanotechnology DNA nanotechnology Human cancer Human cancer Mirror-symmetric DNA polyhedron Mirror-symmetric DNA polyhedron Precision medicine Precision medicine Zigzag-shaped triangle-toothed gear occlude Zigzag-shaped triangle-toothed gear occlude
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| GB/T 7714 | Gao, Qian , He, Tenghang , Chen, Linhuan et al. Triangle-toothed gear occlude-guided universal nanotechnology constructs 3D symmetric DNA polyhedra with high assembly efficiency for precision cancer therapy [J]. | JOURNAL OF COLLOID AND INTERFACE SCIENCE , 2024 , 677 : 1045-1060 . |
| MLA | Gao, Qian et al. "Triangle-toothed gear occlude-guided universal nanotechnology constructs 3D symmetric DNA polyhedra with high assembly efficiency for precision cancer therapy" . | JOURNAL OF COLLOID AND INTERFACE SCIENCE 677 (2024) : 1045-1060 . |
| APA | Gao, Qian , He, Tenghang , Chen, Linhuan , Zhu, Shidan , Li, Congcong , Zeng, Yi et al. Triangle-toothed gear occlude-guided universal nanotechnology constructs 3D symmetric DNA polyhedra with high assembly efficiency for precision cancer therapy . | JOURNAL OF COLLOID AND INTERFACE SCIENCE , 2024 , 677 , 1045-1060 . |
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Electronic cigarette (e-Cig) has been promoted as a safer alternative to traditional cigarette (t-Cig) recently. However, there are limited scientific data on the potential health effects of e-Cig use. In this study, we evaluated the cytotoxicities of e-Cig and t-Cig condensate solutions (e-CigCS and t-CigCS) on human bronchial epithelial cells (16HBE cells) in vitro, and employed the exosome proteomic technique to systematically assess the effects of e-CigCS and t-CigCS on 16HBE cells. Cytotoxicity assay showed 16HBE cells were more sensitive to t-CigCS than e-CigCS. Proteomic analysis demonstrated that there are 431 differential expressed exosomal proteins (DEEPs) in test groups compared to the control air group (P-value<0.05) and t-CigCS has a greater influence than e-CigCS on exosomal protein expression. Bioinformatic analysis showed the DEEPs from the t-Cig group were significantly enriched in pathways in cancer while tobacco-flavored e-Cig (e-Cigt) and menthol-flavored e-Cig (e-Cigm) groups were not. Further validations of some DEEPs, such as NF-kappa B p65, Sulfiredoxin-1(SRXN1) and Thioredoxininteracting protein (TXNIP), were carried out using immunoblot and Real-time PCR analysis, showing that tCig may have a greater influence than e-Cig on tumor development and metastasis. Taken together, the finding reported here strongly support our hypothesis that electronic cigarettes are significantly less toxic compared with traditional cigarette.
Keyword ：
16HBE cells 16HBE cells Cytotoxicity Cytotoxicity Electronic cigarette(e-Cig) Electronic cigarette(e-Cig) Exosome proteomics Exosome proteomics Traditional cigarette(t-Cig) Traditional cigarette(t-Cig)
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| GB/T 7714 | Wang, Weiling , Zeng, Rong , Liu, Min et al. Exosome proteomics study of the effects of traditional cigarettes and electronic cigarettes on human bronchial epithelial cells [J]. | TOXICOLOGY IN VITRO , 2023 , 86 . |
| MLA | Wang, Weiling et al. "Exosome proteomics study of the effects of traditional cigarettes and electronic cigarettes on human bronchial epithelial cells" . | TOXICOLOGY IN VITRO 86 (2023) . |
| APA | Wang, Weiling , Zeng, Rong , Liu, Min , Chen, Mulan , Wei, Shiqin , Li, Bowen et al. Exosome proteomics study of the effects of traditional cigarettes and electronic cigarettes on human bronchial epithelial cells . | TOXICOLOGY IN VITRO , 2023 , 86 . |
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It remains technically challenging to develop a sensitive assay system to isothermally amplify the signal for miRNA detection because of its low abundance in tested sample, sequence similarities and existence in complex biological environments. In this study, using miRNA-21 as target model, a hairpin-inserted cross-shaped DNA nanoprobe (CP) with four functional arms is constructed for the ultrasensitive detection of miRNA via one-step built-in target analogue (BTA) cycle-mediated signal amplification. BTA is pre-locked in one arm of CP probe and inactive. In the presence of target miRNA, BTA can be unlocked and initiate an isothermal amplification process. Utilizing as-designed CP probe, miRNA-21 can be detected to down to 500 fM, and the linear response range spans over five orders of magnitude. The nonspecific signal is less than 1% upon nontarget miRNAs. CP probe exhibits ~six times enhancement in resistance to nuclease degradation and no obvious degradation-induced fluorescence change is detected during the assay period. The recovery yield ranges from 98.2~105.5% in FBS solution. Because of the high sensitivity, desirable specificity, strong anti-interference ability and substantial increase in nuclease resistance, CP probe is a promising tool for the detection of miRNAs in a complex biological milieu.
Keyword ：
Built-in target analogue cycle Built-in target analogue cycle Isothermal Isothermal MicroRNA-21 MicroRNA-21 Palindromic sticky end Palindromic sticky end Signal amplification Signal amplification
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| GB/T 7714 | Jiang, Hao , Wang, Wenqing , Wang, Weijun et al. Hairpin-inserted cross-shaped DNA nanoprobe for ultrasensitive microRNA detection based on built-in target analogue cycle amplification [J]. | TALANTA , 2022 , 250 . |
| MLA | Jiang, Hao et al. "Hairpin-inserted cross-shaped DNA nanoprobe for ultrasensitive microRNA detection based on built-in target analogue cycle amplification" . | TALANTA 250 (2022) . |
| APA | Jiang, Hao , Wang, Wenqing , Wang, Weijun , Xue, Chang , Wang, Lei , Liu, Dengyou et al. Hairpin-inserted cross-shaped DNA nanoprobe for ultrasensitive microRNA detection based on built-in target analogue cycle amplification . | TALANTA , 2022 , 250 . |
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本发明属于生物医药技术领域，具体涉及了血清CYR61‑SNO蛋白作为标志物在制备新型乳腺癌快速检测试剂盒中的应用。本发明首次发现乳腺癌病人血清中CYR61‑SNO蛋白含量显著高于正常健康者，提示其在乳腺癌检测中的价值。并提供了一种基于检测血清中CYR61‑SNO蛋白含量的新型乳腺癌快速检测试剂盒，通过选择性地清除血清中的高丰度干扰蛋白，再富集血清中的巯亚硝基化蛋白，然后采用ELISA法测定血清中CYR61‑SNO的含量，进而完成对乳腺癌患者的检测及筛查。该试剂盒检测样品为外周血，取样方便，操作简单，检测灵敏，具有较好的实施意义。
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| GB/T 7714 | 余素红 , 刘敏 , 曾蓉 et al. 血清CYR61-SNO蛋白作为标志物在制备新型乳腺癌快速检测试剂盒中的应用 : CN202110862469.6[P]. | 2021-07-29 . |
| MLA | 余素红 et al. "血清CYR61-SNO蛋白作为标志物在制备新型乳腺癌快速检测试剂盒中的应用" : CN202110862469.6. | 2021-07-29 . |
| APA | 余素红 , 刘敏 , 曾蓉 , 王伟玲 . 血清CYR61-SNO蛋白作为标志物在制备新型乳腺癌快速检测试剂盒中的应用 : CN202110862469.6. | 2021-07-29 . |
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Aberrant mitophagy has been implicated in a broad spectrum of disorders. PINK1, Parkin, and ubiquitin have pivotal roles in priming mitophagy. However, the entire regulatory landscape and the precise control mechanisms of mitophagy remain to be elucidated. Here, we uncover fundamental mitophagy regulation involving PINK1 and a non-canonical role of the mitochondrial Tu translation elongation factor (TUFm). The mitochondrion-cytosol dual-localized TUFm interacts with PINK1 biochemically and genetically, which is an evolutionarily conserved Parkin-independent route toward mitophagy. A PINK1-dependent TUFm phosphoswitch at Ser222 determines conversion from activating to suppressing mitophagy. PINK1 modulates differential translocation of TUFm because p-S222-TUFm is restricted predominantly to the cytosol, where it inhibits-mitophagy by impeding Atg5-Atg12 formation. The self-antagonizing feature of PINK1/TUFm is critical for the robustness of mitophagy regulation, achieved by the unique kinetic parameters of p-S222-TUFm, p-S65-ubiquitin, and their common kinase PINK1. Our findings provide new mechanistic insights into mitophagy and mitophagy-associated disorders.
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| GB/T 7714 | Lin, Jingjing , Chen, Kai , Chen, Wenfeng et al. Paradoxical Mitophagy Regulation by PINK1 and TUFm [J]. | MOLECULAR CELL , 2020 , 80 (4) : 607-, . |
| MLA | Lin, Jingjing et al. "Paradoxical Mitophagy Regulation by PINK1 and TUFm" . | MOLECULAR CELL 80 . 4 (2020) : 607-, . |
| APA | Lin, Jingjing , Chen, Kai , Chen, Wenfeng , Yao, Yizhou , Ni, Shiwei , Ye, Meina et al. Paradoxical Mitophagy Regulation by PINK1 and TUFm . | MOLECULAR CELL , 2020 , 80 (4) , 607-, . |
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Abstract ：
MicroRNAs (miRNAs) are involved in a variety of biological processes, and the accurate detection of miRNAs is of great importance for early diagnosis of various cancers. Herein, we have developed a highly sensitive method for the intracellular imaging of miRNAs based on a palindromic probe-induced strand displacement amplification (pSDA). The sensing element is a partly complementary hybrid consisting of two DNA components: one fluorescent dye-labeled signaling probe containing a palindromic sequence and loop-based target recognition site and one quencher moiety-attached locking probe. In the presence of target miRNA, the target species can hybridize with the loop site and release the terminal palindromic fragment, initiating the pSDA reaction. Thus, a considerable amount of fluorescent moieties are spatially separated from the quenchers, generating a dramatically enhanced fluorescence signal. As a result, the target miRNAs can be quantified down to 25 pM with the linear response range over four orders of magnitude. The detection specificity is high enough to eliminate the interference from nontarget miRNAs and other biospecies co-existing in samples, and thus the diseased cells are easily distinguished from healthy cells. Strikingly, the pSDA-based system possesses the desirable capability to discriminate tumor cells from healthy cells, indicating a promising diagnostic tool for the detection of cancers and other diseases in early stage.
Keyword ：
Confocal scanning microscopy Confocal scanning microscopy miRNAs miRNAs Palindromic probe-mediated strand displacement amplification (pSDA) Palindromic probe-mediated strand displacement amplification (pSDA) Tumor cells Tumor cells
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| GB/T 7714 | Xu, Huo , Niu, Huimin , Liu, Jingtao et al. Palindromic probe-mediated strand displacement amplification for highly sensitive and selective microRNA imaging [J]. | TALANTA , 2020 , 219 . |
| MLA | Xu, Huo et al. "Palindromic probe-mediated strand displacement amplification for highly sensitive and selective microRNA imaging" . | TALANTA 219 (2020) . |
| APA | Xu, Huo , Niu, Huimin , Liu, Jingtao , Zhang, Yafeng , Yin, Hongwei , Liu, Dengyou et al. Palindromic probe-mediated strand displacement amplification for highly sensitive and selective microRNA imaging . | TALANTA , 2020 , 219 . |
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Successful adhesion of circulating tumor cells (CTCs) to microvascular endothelium of distant metastatic tissue is the key starting step of metastatic cascade that could be effectively chemoprevented as we demonstrated previously. Here, we hypothesize that the hetero-adhesion may produce secretory biomarkers that may be important for both premetastatic diagnosis and chemoprevention. We show that co-incubation of triple-negative breast cancer (TNBC) cell line MDA-MB-231 with human pulmonary microvascular endothelial monolayers (HPMEC) secretes Cyr61 (CCN1), primarily from MDA-MB-231. However, addition of metapristone (RU486 metabolite) to the co-incubation system inhibits Cyr61 secretion probably via the Cyr61/integrin alpha v beta 1 signaling pathway without significant cytotoxicity on both MDA-MB-231 and HPMEC. Transfection of MDA-MB-231 with Cyr61-related recombinant plasmid or siRNA enhances or reduces Cyr61 expression, accordingly. The transfection significantly changes hetero-adhesion and migration of MDA-MB-231, and the changed bioactivities by overexpressed CYR61 could be antagonized by metapristone in vitro. Moreover, the circulating MDA-MB-231 develops lung metastasis in mice, which could be effectively prevented by oral metapristone without significant toxicity. The present study, for the first time, demonstrates that co-incubation of MDA-MB-231 with HPMEC secrets CYR61 probably via the CYR61/integrin alpha(v)beta(1) signaling pathway to promote adhesion-invasion of TNBC (early metastatic step). Metapristone, by interfering the adhesion-invasion process, prevents metastasis from happening.
Keyword ：
CYR61 CYR61 HPMEC co-culture HPMEC co-culture integrin alpha(v)beta(1) integrin alpha(v)beta(1) MDA-MB-231 MDA-MB-231 metapristone metapristone metastasis chemoprevention metastasis chemoprevention
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| GB/T 7714 | Yu, Suhong , Yan, Cuicui , Wu, Wenjing et al. RU486 Metabolite Inhibits CCN1/Cyr61 Secretion by MDA-MB-231-Endothelial Adhesion [J]. | FRONTIERS IN PHARMACOLOGY , 2019 , 10 . |
| MLA | Yu, Suhong et al. "RU486 Metabolite Inhibits CCN1/Cyr61 Secretion by MDA-MB-231-Endothelial Adhesion" . | FRONTIERS IN PHARMACOLOGY 10 (2019) . |
| APA | Yu, Suhong , Yan, Cuicui , Wu, Wenjing , He, Sudan , Liu, Min , Liu, Jian et al. RU486 Metabolite Inhibits CCN1/Cyr61 Secretion by MDA-MB-231-Endothelial Adhesion . | FRONTIERS IN PHARMACOLOGY , 2019 , 10 . |
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本发明提供一种抗菌肽Lchamp2‑3在抗肿瘤细胞转移药物中的应用。通过划痕实验，静态粘附实验，侵袭实验，细胞毒性实验表明，抗菌肽Lchamp2‑3对癌细胞的毒性相对不高，但对癌细胞转移具有显著的抑制作用。本发明中的抗菌肽Lchamp2‑3能够应用于抗肿瘤转移药物的制备中，开发新型抗肿瘤转移候选药物。
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| GB/T 7714 | 余素红 , 吴文静 , 刘敏 et al. 抗菌肽Lchamp2-3在抗肿瘤细胞转移药物中的应用 : CN201910573838.2[P]. | 2019/6/28 . |
| MLA | 余素红 et al. "抗菌肽Lchamp2-3在抗肿瘤细胞转移药物中的应用" : CN201910573838.2. | 2019/6/28 . |
| APA | 余素红 , 吴文静 , 刘敏 , 陈新华 , 母尹楠 , 贾力 . 抗菌肽Lchamp2-3在抗肿瘤细胞转移药物中的应用 : CN201910573838.2. | 2019/6/28 . |
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本发明公开了一种抗菌肽Lchamp2‑3在抗肿瘤细胞增殖药物中的应用。通过体外细胞实验表明，源于大黄鱼的抗菌肽Lchamp2‑3对癌细胞的增殖、凋亡及细胞集落形成具有显著的抑制作用。能够被应用于抗肿瘤细胞增殖药物中，开发新型防治肿瘤候选药物。
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| GB/T 7714 | 余素红 , 陈新华 , 吴文静 et al. 抗菌肽Lchamp2-3在抗肿瘤细胞增殖药物中的应用 : CN201910574493.2[P]. | 2019/6/28 . |
| MLA | 余素红 et al. "抗菌肽Lchamp2-3在抗肿瘤细胞增殖药物中的应用" : CN201910574493.2. | 2019/6/28 . |
| APA | 余素红 , 陈新华 , 吴文静 , 母尹楠 , 刘敏 , 江舟 . 抗菌肽Lchamp2-3在抗肿瘤细胞增殖药物中的应用 : CN201910574493.2. | 2019/6/28 . |
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A perfect microenvironment facilitates the activated circulating tumor cells (CTCs) to spark the adhesion-invasion-extravasation metastatic cascade in their premetastatic niche. Platelet-CTC interaction contributes to the progression of tumor malignancy by protecting CTCs from shear stress and immunological assault, aiding CTCs entrapment in the capillary bed, enabling CTCs to successfully exit the bloodstream and enter the tissue, inducing epithelial-mesenchymal-like transition (EMT), and assisting in the establishment of metastatic foci. To prevent the cascade from sparking, we show that, the multifunctional S-nitrosocaptopril (CapNO) acts on both CTCs and platelets to interrupt platelet/CTCs interplay and adhesion to endothelium, thus inhibiting CTC-based pulmonary metastasis in vivo. The activated platelets cloak cancer HT29 cells, resulting in HT29-exhibiting platelet biomarkers CD61 and P-selectin positive. CapNO inhibits both sialyl Lewis(x) (Slex) expression on HT29 and ADP-induced activation of platelets through P-selectin- and GPIIb/IIIa-dependent mechanisms, confirmed by the corresponding antibody assay. CapNO inhibits platelet- or interleukin (IL)-1 beta-mediated adhesion between HT29 and endothelial cells, and micrometastatic formation in the lungs of immunocompetent syngeneic mouse models. CapNO have also shown the effects of vasodilation, anticoagulation, inhibition of matrix metalloproteinase-2 (MMP2) expression on cancer cells, and inhibition of cell adhesion molecules (CAMs) expression on vascular endothelium. Due to a series of the beneficial effects of CapNO, CTCs remain exposed to the hostile bloodstream environment and are vulnerable to death induced by shear stress and immune elimination. This new discovery provides a basis for CapNO used for cancer metastatic chemoprevention, and might suggest regulation of the CTCs bloodstream microenvironment as a new avenue for cancer metastatic prevention.
Keyword ：
Cancer bloodstream microenvironment Cancer bloodstream microenvironment Cancer metastatic chemoprevention Cancer metastatic chemoprevention Circulating tumor cells Circulating tumor cells Platelet/CTCs interplay Platelet/CTCs interplay S-Nitrosocaptopril S-Nitrosocaptopril
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| GB/T 7714 | Lu, Yusheng , Lian, Shu , Ye, Yuying et al. S-Nitrosocaptopril prevents cancer metastasis in vivo by creating the hostile bloodstream microenvironment against circulating tumor cells [J]. | PHARMACOLOGICAL RESEARCH , 2019 , 139 : 535-549 . |
| MLA | Lu, Yusheng et al. "S-Nitrosocaptopril prevents cancer metastasis in vivo by creating the hostile bloodstream microenvironment against circulating tumor cells" . | PHARMACOLOGICAL RESEARCH 139 (2019) : 535-549 . |
| APA | Lu, Yusheng , Lian, Shu , Ye, Yuying , Yu, Ting , Liang, Haiyan , Cheng, Yunlong et al. S-Nitrosocaptopril prevents cancer metastasis in vivo by creating the hostile bloodstream microenvironment against circulating tumor cells . | PHARMACOLOGICAL RESEARCH , 2019 , 139 , 535-549 . |
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