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学者姓名:林悦
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The persistence of T-2 toxin in food and feed matrices renders it a pervasive contaminant, impacting both human and animal health. Traditional detection methods suffer from cumbersome instrumentation and intricate procedures, rendering on-site detection of T-2 toxin unfeasible. Therefore, we have constructed a real-time detection method for T-2 toxin detection by employing a target-responsive DNA hydrogel in conjunction with potassium iodide starch test paper. This method integrates both colorimetric and distance-based signal outputs, offering a streamlined and effective approach for the on-site detection of T-2 toxin. The specific binding of the target to the aptamer in the DNA hydrogel results in the collapse of the hydrogels structure, which changes the viscosity of the system and released horseradish peroxidase in the hydrogel wrapped, and then produces blue-purple marks of different lengths on the starch iodide papers to achieve the specific detection of T-2 toxin. Under optimized conditions, the assay exhibits a detection range spanning from 10 ng/mL to 10 mg/mL for the toxin, with a detection limit of 12.83 ng/mL. The proposed method has been successfully applied for the detection of real corn samples with satisfied result. Such colorimetric-distance dual signal detection method offers notable advantages, including straightforward operation, clear signal interpretation, and practical utility. Its implementation enables rapid, on-the-spot detection of T-2 toxin, particularly beneficial in resource-limited regions and less developed countries.
Keyword :
Point-of-care testing Point-of-care testing Starch iodide paper Starch iodide paper T-2 toxin T-2 toxin Target-responsive DNA hydrogel Target-responsive DNA hydrogel
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GB/T 7714 | Deng, Ye , Lin, Jiarong , Wang, Jingxuan et al. Dual-signal point-of-care testing method for T-2 toxin utilizing target-responsive DNA hydrogel and starch iodide paper [J]. | MICROCHEMICAL JOURNAL , 2025 , 210 . |
MLA | Deng, Ye et al. "Dual-signal point-of-care testing method for T-2 toxin utilizing target-responsive DNA hydrogel and starch iodide paper" . | MICROCHEMICAL JOURNAL 210 (2025) . |
APA | Deng, Ye , Lin, Jiarong , Wang, Jingxuan , Lin, Yue , Luo, Fang , Weng, Zuquan et al. Dual-signal point-of-care testing method for T-2 toxin utilizing target-responsive DNA hydrogel and starch iodide paper . | MICROCHEMICAL JOURNAL , 2025 , 210 . |
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Filling the microchannel with negatively charged hydrogel can exhibit microsacle ion current rectification (ICR) behavior, which is attributed to the space negative charge and structural asymmetry of hydrogel. In this study, this character had been applied to develop a trypsin sensor for the first time. A hydrogel synthesized with bovine serum albumin (BSA) and glyoxal (BSAG hydrogel) was filled at the tip of microchannel firstly. Subsequently, the BSAG hydrogel-filled microchannel was immersed in a trypsin solution to hydrolyze the BSA within the BSAG hydrogel. This process changes the space charge density and pore size of the BSAG hydrogel-filled microchannel, leading to a change in microscale ICR, which can be used for quantifying trypsin. Then the key parameters affecting the sensing performance such as the concentration of BSA, strength of the electrolyte, pH and reaction time were optimized. The detection range was from 10.0 ng/mL to 100 mu g/mL with a detection limit as low as 2.55 ng/mL (S/N = 3). Due to the distinctive three-dimensional pore structure of the hydrogel and the specificity of trypsin for BSA hydrolysis, the sensor exhibits high sensitivity and specificity, as well as remarkable reproducibility and stability. This sensor has been effectively used to measure trypsin levels in human serum samples.
Keyword :
Hydrogel Hydrogel Microchannel Microchannel Microscale ionic current rectification Microscale ionic current rectification Space negtive charge Space negtive charge Trypsin Trypsin
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GB/T 7714 | Cai, Huabin , Yuan, Runhao , Huang, Shaokun et al. Sensitive trypsin sensor based on the regulation of microscale ionic current rectification by the selectivity hydrolysis of hydrogel filled in microchannel [J]. | TALANTA , 2025 , 285 . |
MLA | Cai, Huabin et al. "Sensitive trypsin sensor based on the regulation of microscale ionic current rectification by the selectivity hydrolysis of hydrogel filled in microchannel" . | TALANTA 285 (2025) . |
APA | Cai, Huabin , Yuan, Runhao , Huang, Shaokun , Huang, Yanling , Lin, Cuiying , Lin, Yue et al. Sensitive trypsin sensor based on the regulation of microscale ionic current rectification by the selectivity hydrolysis of hydrogel filled in microchannel . | TALANTA , 2025 , 285 . |
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Seafood consumption is the major source of total Hg (tHg) and methyl mercury (MeHg) for humans. Lack of broad-representative bio-accessibility of mercury species makes accurate assessment on health risk of seafood's mercury impossible. Herein, the concentrations and in vitro bio-accessibilities of mercury species in 93 seafood samples with 71 different species were extensively investigated. Results indicated that all shellfish and fish samples, and most seaweed samples contained both Hg2+ and MeHg, while some seaweed samples contained only Hg2+. The concentrations of mercury species varied depending on the differences in species/individuals of seafood and sampling regions. MeHg in seafood can be partly de-methylated into Hg2+ during gastrointestinal digestion, which reduced the toxicity of mercury in seafood. The mean demethylation rate of MeHg varied as follows: seaweeds (similar to 62.1 %) > shellfishes/shrimps (similar to 19.7 %) > fishes (similar to 9.2 %). The mean bio-accessibility of Hg2+ and tHg varied as follows: seaweeds (similar to 97.7 % and similar to 90.1 %) > shellfishes/shrimps (similar to 65.1 % and similar to 67.9 %) approximate to fishes (similar to 65.1 % and similar to 66.7 %), while that of MeHg varied as follows: fishes (similar to 57.7 %) > shellfishes/shrimps (50.8 %) > seaweeds (similar to 11.6 %). The simulated calculation of target hazard quotient (THQ) revealed that the health risk of seafood's mercury may be accurately assessed using tHg, not mercury species, even without considering bio-accessibility. This offers a simple but protective approach for assessing the health risk of seafood's mercury. Results of this study provide the potential broad-representative bio-accessibilities of mercury species existing in various kinds of seafood and novel insights for scientifically assessing the health risk of seafood's mercury and revising the mercury limitation in seafood.
Keyword :
Bioavailability Bioavailability Marine organism Marine organism Mercury Mercury Risk assessment Risk assessment Seafood Seafood
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GB/T 7714 | Sun, Chaochen , Yin, Miaomiao , Peng, Ying et al. The characteristic and bio-accessibility evaluation of mercury species in various kinds of seafood collected from Fujian of China for mercury risk assessment [J]. | JOURNAL OF HAZARDOUS MATERIALS , 2025 , 482 . |
MLA | Sun, Chaochen et al. "The characteristic and bio-accessibility evaluation of mercury species in various kinds of seafood collected from Fujian of China for mercury risk assessment" . | JOURNAL OF HAZARDOUS MATERIALS 482 (2025) . |
APA | Sun, Chaochen , Yin, Miaomiao , Peng, Ying , Lin, Chen , Wu, Yongning , Fu, Fengfu et al. The characteristic and bio-accessibility evaluation of mercury species in various kinds of seafood collected from Fujian of China for mercury risk assessment . | JOURNAL OF HAZARDOUS MATERIALS , 2025 , 482 . |
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采用鲁米诺/H2O2 作为微通道电化学发光(ECL)报告体系,构建了一种微通道ECL传感器用于四环素(TC)的检测.鲁米诺/H2O2 ECL体系较低的激发电位可以降低体系钳位电压的影响,从而提高微通道ECL传感器的检测性能.修饰了四环素适配体(TC-aptamer)的微通道可特异性识别并捕获TC,TC带有的正电荷导致微通道内界面负电荷密度降低,使微通道的离子电流增加,导致体系ECL信号增强.对电解质浓度、TC-aptamer修饰液浓度、TC与TC-aptamer的反应时间等条件进行了优化.在最优条件下,体系ECL信号变化值与TC浓度在1.00~200 ng/mL范围内呈良好的线性关系,检出限为0.69 ng/mL.此传感器具有良好的选择性,并成功用于牛奶样本中TC的检测.
Keyword :
四环素 四环素 微通道 微通道 电化学发光 电化学发光 适配体 适配体 鲁米诺 鲁米诺
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GB/T 7714 | 黄少坤 , 谢秀琳 , 蔡华宾 et al. 以鲁米诺/过氧化氢作为报告体系的微通道电化学发光传感器用于四环素的检测 [J]. | 分析化学 , 2025 , 53 (3) : 356-363 . |
MLA | 黄少坤 et al. "以鲁米诺/过氧化氢作为报告体系的微通道电化学发光传感器用于四环素的检测" . | 分析化学 53 . 3 (2025) : 356-363 . |
APA | 黄少坤 , 谢秀琳 , 蔡华宾 , 黄艳玲 , 林悦 , 林振宇 . 以鲁米诺/过氧化氢作为报告体系的微通道电化学发光传感器用于四环素的检测 . | 分析化学 , 2025 , 53 (3) , 356-363 . |
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Dual-signal point-of-care testing (POCT) method relying on multicolor changes have demonstrated a promising improvement in performance. In this work, we have developed a highly sensitive and rapid POCT method for detecting aflatoxin B1 (AFB1), utilizing a dual-signal readout mode based on temperature and multicolor changes. A synthetic enzyme mimics, Al-Cu-Santa Barbara Amorphous material (Al-Cu-SBA), has been used to enable the dual-signal changes of gold nanobipyramids (Au NBPs) for the first time. Specifically, the aptamer was immobilized on magnetic beads, facilitating hybridization with complementary strands functionalized with Al-Cu-SBA and hence forming double-stranded DNA. Upon introducing AFB1 into the system, it bound to the aptamer chain, resulting in the release of Al-Cu-SBA into solution. The liberated Al-Cu-SBA was subsequently collected and used to oxidize 3, 3′, 5, 5′-tetramethylbenzidine (TMB). The oxidized TMB was used to etch Au NBPs, inducing significant color changes. Meanwhile, by leveraging the photothermal properties of Au NBPs, we observed a temperature difference generated upon exposure to infrared laser irradiation. This method exhibited a linear range from 0.5 μg·mL−1 to 100 μg·mL−1, with a detection limit of 0.167 μg·mL−1. The method's applicability was extended to successfully detect AFB1 in both peanut oil and milk samples. Given the application of enzyme mimics and the dual-signal readout integrating temperature and multicolor changes, our study successfully overcomes the limitations inherent in traditional biological enzyme-based methods and the conventional single-signal readout approach. Additionally, it surpasses the limitations associated with dual-signal readout systems that rely solely on monochromatic colorimetric analysis. © 2024 Elsevier B.V.
Keyword :
Aluminum Aluminum Amorphous materials Amorphous materials Colorimetry Colorimetry Copper Copper Enzymes Enzymes Silica Silica
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GB/T 7714 | Zheng, Zhenjie , Zhan, Linxiu , Wang, Liang et al. Aluminium-copper-mesoporous silica molecular sieve-enabled dual-signal point-of-care aptasensor with integrated temperature and multicolor readout [J]. | Sensors and Actuators B: Chemical , 2024 , 418 . |
MLA | Zheng, Zhenjie et al. "Aluminium-copper-mesoporous silica molecular sieve-enabled dual-signal point-of-care aptasensor with integrated temperature and multicolor readout" . | Sensors and Actuators B: Chemical 418 (2024) . |
APA | Zheng, Zhenjie , Zhan, Linxiu , Wang, Liang , Deng, Ye , Lin, Yue , Luo, Fang et al. Aluminium-copper-mesoporous silica molecular sieve-enabled dual-signal point-of-care aptasensor with integrated temperature and multicolor readout . | Sensors and Actuators B: Chemical , 2024 , 418 . |
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A simple and highly sensitive method for detection of miRNA was constructed by combining the high-efficiency thiolated-mediated strand displacement (TMSD) non-enzymatic amplification and the high -reproducibility electrochemiluminescence/electrochemistry (ECL/EC) ratio sensing technique. The EC signal generated by methylene blue (MB) modified on the electrode was used as the internal reference signal, and Ru(phen)(3)(2+) was embedded in DNA nanoparticles produced by the branched hybridization chain reaction as the ECL probe. The presence of target miRNA facilitated the detachment of MB from electrode surface and the modification of ECL beacon on the electrode, resulting in a decrease of the EC signal and an enhancement of the ECL signal. The method of using the ratio of ECL signal to EC signal reduced potential interference and improved reproducibility and sensitivity of the biosensor. The ratio of ECL/EC signals exhibited linear relationship with miRNA-21 concentration in the range from 10 fmol/L to 500 pmol/L, with a detection limit of 6.5 fmol/L, and the relative standard deviation of the assay was reduced from 8.2 % to 4.1 % when determining 1 pmol/L target miRNA-21 compared to that using single ECL signal. This method was preliminarily applied to the detection of miRNA-21 in complex cell samples.
Keyword :
Electrochemiluminescence Electrochemiluminescence Electrochemistry Electrochemistry miRNA miRNA Ratiometric biosensor Ratiometric biosensor Toehold-mediated strand displacement Toehold-mediated strand displacement
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GB/T 7714 | Jin, Xiao-Ya , Zhang, Ying , Lin, Yue et al. Ratiometric Electrochemiluminescence/Electrochemistry Sensor for miRNA Based on Toehold-Mediated Strand Displacement Amplification [J]. | CHINESE JOURNAL OF ANALYTICAL CHEMISTRY , 2024 , 52 (5) : 743-750 . |
MLA | Jin, Xiao-Ya et al. "Ratiometric Electrochemiluminescence/Electrochemistry Sensor for miRNA Based on Toehold-Mediated Strand Displacement Amplification" . | CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 52 . 5 (2024) : 743-750 . |
APA | Jin, Xiao-Ya , Zhang, Ying , Lin, Yue , Lin, Zhen-Yu . Ratiometric Electrochemiluminescence/Electrochemistry Sensor for miRNA Based on Toehold-Mediated Strand Displacement Amplification . | CHINESE JOURNAL OF ANALYTICAL CHEMISTRY , 2024 , 52 (5) , 743-750 . |
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Dual-signal point-of-care testing (POCT) method relying on multicolor changes have demonstrated a promising improvement in performance. In this work, we have developed a highly sensitive and rapid POCT method for detecting aflatoxin B1 (AFB 1 ), utilizing a dual-signal readout mode based on temperature and multicolor changes. A synthetic enzyme mimics, Al-Cu-Santa Barbara Amorphous material (Al-Cu-SBA), has been used to enable the dual-signal changes of gold nanobipyramids (Au NBPs) for the first time. Specifically, the aptamer was immobilized on magnetic beads, facilitating hybridization with complementary strands functionalized with AlCu-SBA and hence forming double-stranded DNA. Upon introducing AFB 1 into the system, it bound to the aptamer chain, resulting in the release of Al-Cu-SBA into solution. The liberated Al-Cu-SBA was subsequently collected and used to oxidize 3, 3 ' , 5, 5 '-tetramethylbenzidine (TMB). The oxidized TMB was used to etch Au NBPs, inducing significant color changes. Meanwhile, by leveraging the photothermal properties of Au NBPs, we observed a temperature difference generated upon exposure to infrared laser irradiation. This method exhibited a linear range from 0.5 mu g & sdot; mL- 1 to 100 mu g & sdot; mL- 1 , with a detection limit of 0.167 mu g & sdot; mL- 1 . The method ' s applicability was extended to successfully detect AFB 1 in both peanut oil and milk samples. Given the application of enzyme mimics and the dual-signal readout integrating temperature and multicolor changes, our study successfully overcomes the limitations inherent in traditional biological enzyme-based methods and the conventional single-signal readout approach. Additionally, it surpasses the limitations associated with dual-signal readout systems that rely solely on monochromatic colorimetric analysis.
Keyword :
Aflatoxin B1 Aflatoxin B1 Al -Cu -Santa Barbara Amorphous Al -Cu -Santa Barbara Amorphous Dual -signal readout Dual -signal readout Gold nanobipyramids Gold nanobipyramids Point -of -care testing Point -of -care testing
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GB/T 7714 | Zheng, Zhenjie , Zhan, Linxiu , Wang, Liang et al. Aluminium-copper-mesoporous silica molecular sieve-enabled dual-signal point-of-care aptasensor with integrated temperature and multicolor readout [J]. | SENSORS AND ACTUATORS B-CHEMICAL , 2024 , 418 . |
MLA | Zheng, Zhenjie et al. "Aluminium-copper-mesoporous silica molecular sieve-enabled dual-signal point-of-care aptasensor with integrated temperature and multicolor readout" . | SENSORS AND ACTUATORS B-CHEMICAL 418 (2024) . |
APA | Zheng, Zhenjie , Zhan, Linxiu , Wang, Liang , Deng, Ye , Lin, Yue , Luo, Fang et al. Aluminium-copper-mesoporous silica molecular sieve-enabled dual-signal point-of-care aptasensor with integrated temperature and multicolor readout . | SENSORS AND ACTUATORS B-CHEMICAL , 2024 , 418 . |
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A homogeneous electrochemiluminescence (ECL) sensor for hyaluronidase (HAase) detection was developed by controlling the coating of hyaluronic acid (HA) on the surface of magnetic ECL nanoparticles (NPs). Initially, Fe3O4@Ru@SiO2 NPs with magnetic properties were synthesized and subsequently coated with HA (Fe3O4@Ru@SiO2-HA). The properties of HA, such as its negative charge and viscosity, induced strong electrostatic repulsion and reduced electron transfer efficiency between the Fe3O4@Ru@SiO2-HA NPs and the negatively charged electrodes. Furthermore, this coating hindered the approach of co-reactants to the NPs, resulting in a diminished ECL signal. In the presence of HAase, HA is hydrolyzed, releasing the Fe3O4@Ru@SiO2 NPs. Magnetic separation minimizes background interference and allows the magnetic ECL NPs to diffuse freely in solution, facilitating electron transfer and reactant interaction, thereby leading to detectable signal changes. The sensor exhibited a linear response to HAase concentration ranging from 5.0 to 60 U/mL, with a detection limit of 1.8 U/mL. This method is rapid, straightforward, and effectively leverages the inherent properties of HA, making it suitable for broad applications in the detection of proteins, enzymes, and other biomarkers.
Keyword :
Homogeneous electrochemiluminescence Homogeneous electrochemiluminescence Hyaluronic acid Hyaluronic acid Hyaluronidase Hyaluronidase Magnetic separation Magnetic separation
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GB/T 7714 | Wang, Weijia , Luo, Peiqing , Wang, Juan et al. Homogeneous electrochemiluminescence biosensor for hyaluronidase based on the regulation of hyaluronic acid coating on magnetic nanoparticles [J]. | MICROCHEMICAL JOURNAL , 2024 , 207 . |
MLA | Wang, Weijia et al. "Homogeneous electrochemiluminescence biosensor for hyaluronidase based on the regulation of hyaluronic acid coating on magnetic nanoparticles" . | MICROCHEMICAL JOURNAL 207 (2024) . |
APA | Wang, Weijia , Luo, Peiqing , Wang, Juan , Hong, Ming , Lu, Fen , Zeng, Ronghao et al. Homogeneous electrochemiluminescence biosensor for hyaluronidase based on the regulation of hyaluronic acid coating on magnetic nanoparticles . | MICROCHEMICAL JOURNAL , 2024 , 207 . |
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Homogeneous electrochemiluminescence (ECL) has gained attention for its simplicity and stability. However, false positives due to solution background interference pose a challenge. To address this, magnetic ECL nanoparticles (Fe3O4@Ru@SiO2 NPs) were synthesized, offering easy modification, magnetic separation, and stable luminescence. These were utilized in an ECL sensor for miRNA-155 (miR-155) detection, with locked DNAzyme and substrate chain (mDNA) modified on their surface. The poor conductivity of long-chain DNA significantly impacts the conductivity and electron transfer capability of Fe3O4@Ru@SiO2 NPs, resulting in weaker ECL signals. Upon target presence, unlocked DNAzyme catalyzes mDNA cleavage, leading to shortened DNA chains and reduced density. In contrast, the presence of short-chain DNA has minimal impact on the conductivity and electron transfer capability of Fe3O4@Ru@SiO2 NPs. Simultaneously, the material surface's electronegativity decreases, weakening the electrostatic repulsion with the negatively charged electrode, resulting in the system detecting stronger ECL signals. This sensor enables homogeneous ECL detection while mitigating solution background interference through magnetic separation. Within a range of 100 fM to 10 nM, the sensor exhibits a linear relationship between ECL intensity and target concentration, with a 26.91 fM detection limit. It demonstrates high accuracy in clinical sample detection, holding significant potential for clinical diagnostics. Future integration with innovative detection strategies may further enhance sensitivity and specificity in biosensing applications.
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GB/T 7714 | Lin, Yue , Luo, Peiqing , Luo, Fang et al. High-Sensitivity Homogeneous Detection of miRNA-155 Governed by DNA Walker-Regulated Surface DNA Density of Magnetic Electrochemiluminescence Nanoparticles [J]. | ANALYTICAL CHEMISTRY , 2024 , 96 (33) : 13710-13718 . |
MLA | Lin, Yue et al. "High-Sensitivity Homogeneous Detection of miRNA-155 Governed by DNA Walker-Regulated Surface DNA Density of Magnetic Electrochemiluminescence Nanoparticles" . | ANALYTICAL CHEMISTRY 96 . 33 (2024) : 13710-13718 . |
APA | Lin, Yue , Luo, Peiqing , Luo, Fang , Lin, Cuiying , Wang, Jian , Qiu, Bin et al. High-Sensitivity Homogeneous Detection of miRNA-155 Governed by DNA Walker-Regulated Surface DNA Density of Magnetic Electrochemiluminescence Nanoparticles . | ANALYTICAL CHEMISTRY , 2024 , 96 (33) , 13710-13718 . |
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Seaweeds consumption is one of main internal exposure sources of arsenic for human. However, the absence of representative bio-availabilities of arsenic species makes the accurate assessment of arsenic health risk originating from seaweeds consumption impossible. Herein, the arsenic species in various seaweeds collected from Fujian of China were investigated, and the bio-accessibilities/bio-availabilities of arsenic species existing in seaweeds were evaluated in vitro and in vivo . Results revealed that in vitro bio-availabilities of arsenic species presenting in seaweeds, which obtained with Caco-2 cells, were lower than those of pure arsenic standards, and varied with order of inorganic arsenic (iAs) > dimethylarsinic acid (DMA) approximate to arsenobetaine (AsB) > arsenosugars. During gastrointestinal digestion of mice, As( 5 + )was partly methylated into monomethylarsonic acid (MMA) and DMA, which makes the in vivo bioavailability of iAs (-31.8 %) obtained with mouse metabolic experiment is much higher than its in vitro bio-availability (-10.3 %). The in vivo bio-availabilities of DMA and total arsenic (tAs) are similar to their in vitro bio-availabilities. As the dominant arsenic species in most seaweeds, arsenosugars have an -0.0 % of in vivo bioavailability and only a -3.7 % of in vitro bioavailability. The simulated calculation of target hazard quotient (THQ) and target cancer risk (TR) revealed that the arsenic risk originating from seaweeds was greatly degraded by taking into consideration of arsenic species and bio-availabilities, and all seaweeds collected from Fujian are safety for consumption. The simulated calculation also revealed that arsenic risk of seaweeds can be also more accurately assessed based on tAs together with bioavailability, which provides a simple but accurate and protective method for the risk assessment of arsenic originating from seaweeds. Our work provides the possible representative bio-availabilities of arsenic species presenting in seaweeds for accurately assessing arsenic risk of seaweeds, and novel insights into the bio-availabilities of arsenic in animal.
Keyword :
Arsenic Arsenic Bio-accessibility Bio-accessibility Bio-availability Bio-availability Risk assessment Risk assessment Seafood Seafood
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GB/T 7714 | Lin, Yue , Liu, Junfeng , Sun, Ying et al. Bio-accessibility and bio-availability evaluation of each arsenic species existing in various edible seaweeds in vitro and in vivo for arsenic risk assessment [J]. | SCIENCE OF THE TOTAL ENVIRONMENT , 2024 , 946 . |
MLA | Lin, Yue et al. "Bio-accessibility and bio-availability evaluation of each arsenic species existing in various edible seaweeds in vitro and in vivo for arsenic risk assessment" . | SCIENCE OF THE TOTAL ENVIRONMENT 946 (2024) . |
APA | Lin, Yue , Liu, Junfeng , Sun, Ying , Chen, Shilong , Chen, Jianlang , Fu, Fengfu . Bio-accessibility and bio-availability evaluation of each arsenic species existing in various edible seaweeds in vitro and in vivo for arsenic risk assessment . | SCIENCE OF THE TOTAL ENVIRONMENT , 2024 , 946 . |
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