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学者姓名：黄衍堂
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Abstract ：
Lensless imaging microscopy has gained extensive application with the merits of system compactness and cost efficiency; however, its spatial resolution is usually compromised compared to conventional lens-based microscopes. To further enhance the spatial resolution, we built a lensless imaging system integrating a phase mask and a CMOS image sensor, and employed fluorescence fluctuation super-resolution microscopy (FF-SRM) algorithms to fully exploit the fluorescence intermittency (FI) characteristics of fluorescent molecules for high-resolution lensless image reconstruction. The study demonstrates that lensless image sequences processed by the Wiener deconvolution method can effectively retain the original fluorescence intermittency information, allowing for high-resolution reconstruction using FF-SRM algorithms. Furthermore, by combining expansion microscopy (ExM) and leveraging multi-algorithm synergy, we obtained additional improvements in spatial resolution and image quality for lensless imaging, facilitating clear visualization of biological subcellular organelles. This scheme offers a new pathway to achieve high spatial resolution imaging with practical advantages in simplicity and affordability.
Keyword ：
fluorescence intermittency fluorescence intermittency lensless imaging lensless imaging multi-algorithm synergy multi-algorithm synergy super-resolution reconstruction super-resolution reconstruction
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| GB/T 7714 | Zeng, Zhiping , Chen, Xinyi , Xu, Biqing et al. High-Resolution Lensless Microscopy Imaging Based on Fluorescence Intermittency [J]. | JOURNAL OF BIOPHOTONICS , 2025 . |
| MLA | Zeng, Zhiping et al. "High-Resolution Lensless Microscopy Imaging Based on Fluorescence Intermittency" . | JOURNAL OF BIOPHOTONICS (2025) . |
| APA | Zeng, Zhiping , Chen, Xinyi , Xu, Biqing , Qiu, Jin , Huang, Yantang , Xu, Canhua . High-Resolution Lensless Microscopy Imaging Based on Fluorescence Intermittency . | JOURNAL OF BIOPHOTONICS , 2025 . |
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With the increasing demand for temperature sensing and fluorescent anti-counterfeiting applications, there is an urgent need to develop new environmentally friendly materials with multi-peak fluorescence and high stability. In this study, Cs2NaErCl6: Yb3+, Dy3+ double perovskite microcrystals (MCs) are successfully synthesized via a simple 'dissolve-dry' method. The fluorescence intensity ratio technique is employed to assess the temperature sensing performance of these samples, achieving maximum relative sensitivity are 3.27 %/K (up-conversion) and 1.23 %/K (down-conversion), respectively. Because these MCs presents yellow or green emission colors under different excitation, we design an advanced anti-counterfeiting pattern based on the MCs, which greatly improving the security of anti-counterfeiting. The above results show that the sample is dual-mode temperature sensing materials with high sensitivity. In addition, it has great application prospects in dual-mode fluorescent anti-counterfeiting. © 2025 Elsevier B.V.
Keyword ：
Microcrystals Microcrystals Thermocouples Thermocouples
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| GB/T 7714 | Gong, Chenglin , Lin, Lin , Wu, Yingzhen et al. Dual-mode temperature sensing and fluorescent anti-counterfeiting utilizing Cs2NaErCl6: Yb3+, Dy3+ double perovskite microcrystals [J]. | Journal of Luminescence , 2025 , 284 . |
| MLA | Gong, Chenglin et al. "Dual-mode temperature sensing and fluorescent anti-counterfeiting utilizing Cs2NaErCl6: Yb3+, Dy3+ double perovskite microcrystals" . | Journal of Luminescence 284 (2025) . |
| APA | Gong, Chenglin , Lin, Lin , Wu, Yingzhen , Zhang, Yanan , Chen, Xin , Feng, Zhuohong et al. Dual-mode temperature sensing and fluorescent anti-counterfeiting utilizing Cs2NaErCl6: Yb3+, Dy3+ double perovskite microcrystals . | Journal of Luminescence , 2025 , 284 . |
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Fluorescence fluctuation-based super-resolution microscopy (FF-SRM) is an economical and widely applicable technique that significantly enhances the spatial resolution of fluorescence imaging by capitalizing on fluorescence intermittency. However, each variant of FF-SRM imaging has inherent limitations. This study proposes a super-resolution reconstruction strategy (synSRM) by synergizing multiple variants of the FF-SRM approach to address the limitations and achieve high-quality and high-resolution imaging. The simulation and experimental results demonstrate that, compared to images reconstructed using single FF-SRM algorithms, by selecting suitable synSRM routes according to various imaging conditions, further improvements of the spatial resolution and image reconstruction quality can be obtained for super-resolution fluorescence imaging. (c) 2024 Optica Publishing Group under the terms of the Optica Open Access Publishing Agreement
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| GB/T 7714 | Zeng, Zhiping , Xu, Biqing , Qiu, Jin et al. Fluorescence super-resolution microscopy via fluctuation-based multi-route synergy [J]. | BIOMEDICAL OPTICS EXPRESS , 2024 , 15 (10) : 5886-5900 . |
| MLA | Zeng, Zhiping et al. "Fluorescence super-resolution microscopy via fluctuation-based multi-route synergy" . | BIOMEDICAL OPTICS EXPRESS 15 . 10 (2024) : 5886-5900 . |
| APA | Zeng, Zhiping , Xu, Biqing , Qiu, Jin , Chen, Xinyi , Huang, Yantang , Xu, Canhua . Fluorescence super-resolution microscopy via fluctuation-based multi-route synergy . | BIOMEDICAL OPTICS EXPRESS , 2024 , 15 (10) , 5886-5900 . |
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The upconversion luminescence at the visible band was excited by the high power density whispering gallery mode (WGM) on the surface of the microsphere. Under the WGM excitation, Er3+ of NaYF4:Er3+ directly absorbed three photons at 1525 nm, followed by the generation of upconversion luminescence. We used WS2 quantum dots (QDs) as donors and NaYF4:Er3+ upconversion nanoparticles (UCNPs) as acceptors to enhance upconversion luminescence by Forster resonant energy transfer (FRET). The physical mixture composed of monolayer WS2 and NaYF4:Er3+ nanoparticles was coated on SiO2 microsphere by immersion method. Excited by high power density WGM, three photons were absorbed by WS2-QDs based on the quantum size effect to generate excited electrons as donors and UCNPs as acceptors based on FRET, subsequently, the energy of the electrons were transferred to the luminescence center Er3+ to improve the upconversion luminescence efficiency. The mechanism model of upconversion luminescence enhancement based on FRET of WS2-NaYF4:Er3+ was proposed, and the enhancement effect was experimentally studied and demonstrated. To study the distance dependence of energy transfer between QD and UCNP pairs, we covered the surface of the silica microsphere with a controlled concentration of WS2 mixed with NaYF4:Er3+ nano-particles. When the mixed concentration of WS2-QDs was 0.15%, the upconversion luminescence reached the highest efficiency. The results showed that a twofold upconversion luminescence enhancement can be experimentally achieved. The proposed approach for enhancing the upconversion luminescence could benefit the improvement of imaging signal-to-noise ratio (SNR) using UCNPs for fluorescent labels in life sciences.
Keyword ：
Enhanced upconversion luminescence Enhanced upconversion luminescence Forster resonant energy transfer (FRET) Forster resonant energy transfer (FRET) Three-photon absorption Three-photon absorption Whispering gallery mode (WGM) Whispering gallery mode (WGM)
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| GB/T 7714 | Huang, Yantang , He, Xinyu , Liao, Songsong et al. Enhanced upconversion luminescence of NaYF4:Er nanocrystals based on the synergy between whispering gallery mode excitation and forster resonant energy transfer [J]. | OPTICAL MATERIALS , 2024 , 150 . |
| MLA | Huang, Yantang et al. "Enhanced upconversion luminescence of NaYF4:Er nanocrystals based on the synergy between whispering gallery mode excitation and forster resonant energy transfer" . | OPTICAL MATERIALS 150 (2024) . |
| APA | Huang, Yantang , He, Xinyu , Liao, Songsong , Tu, Bifeng , Xu, Canhua , Huang, Wenqi et al. Enhanced upconversion luminescence of NaYF4:Er nanocrystals based on the synergy between whispering gallery mode excitation and forster resonant energy transfer . | OPTICAL MATERIALS , 2024 , 150 . |
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Optical microcavity can confine photons to a tiny scale for a long time, and is an integrated, low-loss, small mode volume resonant cavity. The resonant frequency of WGM microcavities can be tuned by changing the refractive index and size of the cavity.Based on previous research, this paper proposes a micro-mushroom-shaped microcavity and illustrates the preparation method and characteristics of optical micro-mushroom cavities made of quartz materials. Through the photo-thermal effect, heated light was injected in along the handle of the micro-mushroom cavity, and the frequency tuning of the micro-cavity was thus achieved through the photo-thermal-optical conversion. © 2024 SPIE.
Keyword ：
Microcavities Microcavities Natural frequencies Natural frequencies Refractive index Refractive index
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| GB/T 7714 | Liao, Songsong , Tu, Bifeng , Xu, Huiyang et al. All-optical tuned optical micro-mushroom cavity resonance wavelength [C] . 2024 . |
| MLA | Liao, Songsong et al. "All-optical tuned optical micro-mushroom cavity resonance wavelength" . (2024) . |
| APA | Liao, Songsong , Tu, Bifeng , Xu, Huiyang , Feng, Baihe , Huang, Yantang . All-optical tuned optical micro-mushroom cavity resonance wavelength . (2024) . |
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According to the principle of blackbody radiation, the experimental exploration of high temperature optical fiber temperature sensor was carried out. The high temperature resistant calcium tungstate material was used as the blackbody radiation material to be made the optical fiber radiation probe. Optical power meter was used to measure the optical power of 980nm wavelength in every 10°C in the range of 150-800°C. The fourth-order polynomial was used to fit the relationship between the radiation optical power and the temperature, and the fourth-order optical power and temperature expression with a fitting degree of 0.99953 was obtained. The temperature measurement accuracy was within 1 % in the temperature range of 400-800 ℃. © 2024 SPIE.
Keyword ：
Heat radiation Heat radiation Optical fibers Optical fibers Temperature measurement Temperature measurement Temperature sensors Temperature sensors Tungsten compounds Tungsten compounds
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| GB/T 7714 | He, Pengyu , Wu, Luzhu , Liu, Huiyuan et al. High temperature optical fiber temperature sensor based on blackbody thermal radiation [C] . 2024 . |
| MLA | He, Pengyu et al. "High temperature optical fiber temperature sensor based on blackbody thermal radiation" . (2024) . |
| APA | He, Pengyu , Wu, Luzhu , Liu, Huiyuan , Feng, Baihe , Huang, Yantang . High temperature optical fiber temperature sensor based on blackbody thermal radiation . (2024) . |
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In this paper, Cs2NaErCl6 double perovskites microcrystals (MCs) co-doped with Yb3+ and Bi3+ are successfully synthesized by a simple "dissolving-drying" method. When excited at 379 nm, 808 nm and 980 nm, the MCs show strong red, green and yellow light, respectively, which can be well distinguished by the naked eyes. On this basis, two kinds of systems are designed with doped/undoped MCs for triple-mode fluorescent anti-counterfeiting and information encryption of numbers and characters. The results indicate that our MCs are not only beneficial to the application of fluorescent anti-counterfeiting, but also greatly improve the security of anti-counterfeiting. In addition, we evaluate the temperature sensing performance of these MCs by fluorescence intensity ratio technique. The samples show a high relative sensitivity as 3.8 %/K at 243 K. The results show that the samples have multi-functional applications in the fields of fluorescent anti-counterfeiting and temperature sensing, and this work will broaden the application fields of lead-free double perovskites in fluorescent anti-counterfeiting and temperature sensing.
Keyword ：
Double perovskites Double perovskites Fluorescent anti-counterfeiting Fluorescent anti-counterfeiting High-sensitivity High-sensitivity Microcrystals Microcrystals Temperature sensing Temperature sensing
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| GB/T 7714 | Gong, Chenglin , Lin, Lin , Wu, Yingzhen et al. Triple-mode fluorescent anti-counterfeiting and temperature sensing properties of lead-free double perovskites Cs2NaErCl6: Yb3+ , Bi3+ microcrystals [J]. | JOURNAL OF ALLOYS AND COMPOUNDS , 2024 , 1008 . |
| MLA | Gong, Chenglin et al. "Triple-mode fluorescent anti-counterfeiting and temperature sensing properties of lead-free double perovskites Cs2NaErCl6: Yb3+ , Bi3+ microcrystals" . | JOURNAL OF ALLOYS AND COMPOUNDS 1008 (2024) . |
| APA | Gong, Chenglin , Lin, Lin , Wu, Yingzhen , Zhang, Yanan , Feng, Zhuohong , Wang, Zhezhe et al. Triple-mode fluorescent anti-counterfeiting and temperature sensing properties of lead-free double perovskites Cs2NaErCl6: Yb3+ , Bi3+ microcrystals . | JOURNAL OF ALLOYS AND COMPOUNDS , 2024 , 1008 . |
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In this work, a ligand-free luminescent hydrogel is used as a visual ratiometric fluorescence sensor to detect rareearth (RE) ions for the first time. This hydrogel is easy to be prepared, environmentally friendly, and has high luminescent efficiency. It is found that the fluorescence intensity and emission color of the hydrogel immediately change significantly when solution of active RE3+ 3 + ions is added without any treatment. According to the linear relationship between the fluorescence intensity ratio and the concentration of RE3+ 3 + solutions, the detection limit is calculated to be as low as 0.94 mu M. The detection of RE ions has strong anti-interference to other ions. Therefore, this sensor can be used for preliminary field exploration of RE ores and detection of waste containing small amounts of RE ions.
Keyword ：
Fluorescence sensor Fluorescence sensor Ligand-free hydrogel Ligand-free hydrogel Rare-earth ions Rare-earth ions Visual detection Visual detection
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| GB/T 7714 | Zhang, Yanan , Lin, Lin , Wu, Yingzhen et al. A ligand-free hydrogel as a visual fluorescence sensor for detection of rare-earth ions [J]. | OPTICS COMMUNICATIONS , 2024 , 570 . |
| MLA | Zhang, Yanan et al. "A ligand-free hydrogel as a visual fluorescence sensor for detection of rare-earth ions" . | OPTICS COMMUNICATIONS 570 (2024) . |
| APA | Zhang, Yanan , Lin, Lin , Wu, Yingzhen , Gong, Chenglin , Feng, Zhuohong , Wang, Zhezhe et al. A ligand-free hydrogel as a visual fluorescence sensor for detection of rare-earth ions . | OPTICS COMMUNICATIONS , 2024 , 570 . |
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Objective Due to its economic advantages, convenience of use, and wide applicability, fluorescence fluctuation-based superresolution microscopy has rapidly advanced in recent years and has garnered increased attention and application. Compared with other super-resolution imaging techniques, fluorescence fluctuation-based super-resolution microscopy offers lower system costs and is particularly suitable for imaging live cells, demonstrating exceptional performance in observing subcellular structures and monitoring dynamic processes. Specifically, variations in the fluorescence fluctuation characteristics significantly affect the quality of the superresolution reconstructed images. Therefore, a systematic investigation of image quality under various fluorescence fluctuation conditions is crucial for identifying the most suitable super-resolution imaging approach. These fluorescence fluctuation conditions include parameters such as the number of image-acquisition frames, signal-to-noise ratio, bright-to-dark state probability, and brightto- dark fluorescence intensity ratio, which directly affect image clarity, the signal-to-noise ratio, and accuracy. Thoroughly examining these conditions, we can effectively select and optimize the super-resolution imaging method that meet specific research requirements and experimental conditions. Methods We developed a fluorescence fluctuation-based super-resolution comprehensive imaging reconstruction platform using MATLAB. This platform integrates four super-resolution methods, namely, SOFI, MSSR, MUSICAL, and SPARCOM, and can simulate fluorescence fluctuation signals under different conditions while simultaneously applying multiple super-resolution methods to generate datasets. The platform also supports the import and reconstruction of experimental data and presents the reconstruction results clearly and intuitively on the platform interface, thus allowing users to conveniently compare the imaging results of different approaches. A comprehensive image-quality assessment is then conducted on these simulated datasets. This study used four sets of data under different fluorescence fluctuation conditions and quantitatively analyzed the quality of the reconstructed images generated by the four super-resolution algorithms using five evaluation parameters: the resolution-scaled Pearson coefficient (RSP), resolutionscaled error (RSE), relative error of strength (K), signal-to-noise ratio (SNR), and resolution (R). These five parameters were used to determine the image reconstruction consistency, reconstruction error, image reconstruction uniformity, SNR of the reconstructed images, and improvements in the reconstructed image resolution. In addition, to assess the quality of images reconstructed by the super-resolution algorithms more comprehensively and objectively, this study assigned specific weights to these five evaluation parameters and defined a comprehensive evaluation factor (CEF). The weights were determined based on the relative importance of each parameter in the super-resolution imaging technology to ensure the contribution of each parameter was accurately reflected. To facilitate a better comparison of the performances of the four super-resolution algorithms, this study integrated a multilayer perceptron model with a CEF and datasets generated under various fluorescence fluctuation conditions. The model can determine the superresolution image reconstruction method that best performs under various fluorescence fluctuation conditions by learning and analyzing the performance of different algorithms and outputting an optimal algorithm selection. In short, this model considers different fluorescence fluctuation conditions as inputs and uses a comprehensive evaluation factor of the reconstructed results from various superresolution algorithms as outputs. Results and Discussions Under the fluorescence fluctuation super-resolution comprehensive imaging reconstruction platform, fluorescence signals under varying fluorescence fluctuation conditions were generated. Super-resolution algorithms were applied to reconstruct the datasets and calculate their CEF values; some simulation results are presented in Table 1. The SPARCOM method demonstrates the best performance in terms of resolution and denoising capability, achieving a spatial resolution of up to 44 nm. However, this method relies heavily on the sparsity of image sequences for super-resolution reconstruction and struggles to reconstruct images accurately when the bright-state probability of the fluorescence fluctuation signal is too high or the bright-dark ratio is too low. The MUSICAL method, which has lower resolution capabilities, offers superior denoising performance but exhibits poor image reconstruction consistency, uniformity, and a longer reconstruction time. The MSSR method has moderate resolution capabilities but exhibits superior image reconstruction consistency and uniformity and can be combined with other super-resolution algorithms to obtain higher-quality super-resolution images. Although the SOFI method has lower resolution and denoising capabilities, it exhibits good image reconstruction consistency and uniformity and exhibits a higher image reconstruction rate. A multilayer perceptron model was constructed with fluorescence fluctuation characteristics as inputs and the CEF values of different algorithms as outputs. An analysis of the generated and evaluated datasets showed that the constructed model achieves an accuracy of 92.3%, indicating reliable classification and recognition capabilities and enabling intelligent selection of the most suitable superresolution image reconstruction method under varying fluorescence fluctuation signal conditions. Conclusions We developed a comprehensive super-resolution image reconstruction platform using MATLAB, which implements signal generation and super-resolution image reconstruction functions under various fluorescence fluctuation conditions. The performances of multiple super-resolution algorithms across different fluorescence fluctuation scenarios were systematically evaluated. Leveraging of the dataset generated by the software platform enabled us to introduce a multi-layer perceptron model for intelligent algorithm selection. This in turn allowed for accurate classification and identification of the optimal super-resolution technique. This approach enhances research efficiency and assists researchers in selecting the most suitable fluorescence fluctuation method for various subcellular super-resolution imaging studies. The approach can further advance the application of fluorescence fluctuation-based superresolution imaging techniques for efficient investigation of the ultrafine structures of various biological subcellular organelles. © 2024 Science Press. All rights reserved.
Keyword ：
Failure analysis Failure analysis Fluorescence imaging Fluorescence imaging Fluorescence microscopy Fluorescence microscopy Health risks Health risks Image acquisition Image acquisition Image denoising Image denoising Image quality Image quality Image reconstruction Image reconstruction Image resolution Image resolution Negative bias temperature instability Negative bias temperature instability Photointerpretation Photointerpretation Reliability analysis Reliability analysis Software reliability Software reliability Thermography (imaging) Thermography (imaging)
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| GB/T 7714 | Zeng, Zhiping , Xu, Biqing , Qiu, Jin et al. Fluorescence Fluctuation-Based Super-Resolution Microscopic Imaging Based on a Multilayer Perceptron [J]. | Chinese Journal of Lasers , 2024 , 51 (21) . |
| MLA | Zeng, Zhiping et al. "Fluorescence Fluctuation-Based Super-Resolution Microscopic Imaging Based on a Multilayer Perceptron" . | Chinese Journal of Lasers 51 . 21 (2024) . |
| APA | Zeng, Zhiping , Xu, Biqing , Qiu, Jin , Chen, Xinyi , Xu, Canhua , Huang, Yantang . Fluorescence Fluctuation-Based Super-Resolution Microscopic Imaging Based on a Multilayer Perceptron . | Chinese Journal of Lasers , 2024 , 51 (21) . |
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A flameproof optical fiber liquefied petroleum gas sensor was designed and fabricated. Bare-core CdSe and CdSe@ZnSeS@ZnS@CdZnS were modified with pentafluorobenzoic acid (FBA) and encapsulated in PMMA as fluorescent probes for liquefied petroleum gas, enhancing the quantum dots' response to liquefied petroleum gas detection. Both materials exhibited significant fluorescence quenching in the presence of liquefied petroleum gas, with the Stern-Volmer plot showing a good linear relationship with the gas. A detection optical path was designed, and the relationship between fluorescence intensity and gas concentration was fitted with an expression with a sensitivity of 0.081 (I0/I100-1), enabling real-time detection of liquefied petroleum gas. © Published under licence by IOP Publishing Ltd.
Keyword ：
Fiber optic chemical sensors Fiber optic chemical sensors Gasoline Gasoline Layered semiconductors Layered semiconductors Liquefied petroleum gas Liquefied petroleum gas Nanocrystals Nanocrystals Nanorings Nanorings Optical fiber fabrication Optical fiber fabrication Optical fibers Optical fibers Photonics Photonics Quenching Quenching Selenium compounds Selenium compounds Zinc Selenide Zinc Selenide
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| GB/T 7714 | Yu, Wenwei , Yang, Qiaoyun , Chen, Yujing et al. Optical fiber liquefied petroleum gas sensor based on the fluorescence quenching of CdSe quantum dot [C] . 2024 . |
| MLA | Yu, Wenwei et al. "Optical fiber liquefied petroleum gas sensor based on the fluorescence quenching of CdSe quantum dot" . (2024) . |
| APA | Yu, Wenwei , Yang, Qiaoyun , Chen, Yujing , Li, Hongjie , Huang, Yantang . Optical fiber liquefied petroleum gas sensor based on the fluorescence quenching of CdSe quantum dot . (2024) . |
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