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学者姓名：阮成旭
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Abstract ：
Intestinal enteritis is a main issue in crucian carp production which results in massive economic loss. Traditional antibiotics used for disease prevention of crucian carp (Carassius carassius) have been banned, thus an alternative approach needs to be identified. In this study, the bioactive peptide was evaluated as a diet supplement for preventing intestinal inflammation in crucian carp. Intestinal inflammation was induced by intrarectal admin-istration of a 2,4,6-trinitrobenzene sulfonic acid (TNBS) solution. The fish samples were fed with different diets for 14 days. The disease activity index (DAI), which included, fish swimming, food intake, anal inflammation, body surface, and ascites was determined daily. Intestine segments were stained with haematoxylin and eosin (H. E.) for histopathological analysis. The expression of cytokines, including interleukin-1 beta (IL-1 beta), interleukin-8 (IL -8), tumor necrosis factor alpha (TNF-alpha), and myeloperoxidase (MPO) in crucian carp were determined. In TNBS-induced groups, the DAI scores were dramatically increased compared to the control group. The histopatho-logical analysis showed that the damage of the fish intestine after the injection of TNBS. The relative expression levels of pro-inflammation cytokines (TNF-alpha, IL-1 beta, IL-8, MPO) were significantly increased compared to the control group on day 1. In the TNBS-induced group feed with a diet supplemented with bioactive peptide, the symptoms of intestinal inflammation were relieved on day 3 and the mRNA expression levels of pro -inflammation cytokines (TNF-alpha, IL-1 beta, IL-8, MPO) were reduced compared to day 1. On day 7, the fish sam-ples enrofloxacin group and bioactive peptide group were recovered from TNBS-induced intestinal inflammation. This study showed that the fish diet supplemented with bioactive peptide could help to prevent and recover from intestinal inflammation. Thus, the bioactive peptide can be used as a replacement for antibiotics to prevent disease in aquaculture production.
Keyword ：
Bioactive peptide Bioactive peptide Crucian carp Crucian carp Histology Histology Proinfilammatory molecules Proinfilammatory molecules TNBS TNBS
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| GB/T 7714 | Huang, Meijuan , Wei, Xinyao , Wu, Tiecheng et al. Inhibition of TNBS-induced intestinal inflammation in crucian carp (Carassius carassius) by oral administration of bioactive Bioactive food derived peptides [J]. | FISH & SHELLFISH IMMUNOLOGY , 2022 , 131 : 999-1005 . |
| MLA | Huang, Meijuan et al. "Inhibition of TNBS-induced intestinal inflammation in crucian carp (Carassius carassius) by oral administration of bioactive Bioactive food derived peptides" . | FISH & SHELLFISH IMMUNOLOGY 131 (2022) : 999-1005 . |
| APA | Huang, Meijuan , Wei, Xinyao , Wu, Tiecheng , Li, Mengyan , Zhou, Lei , Chai, Libing et al. Inhibition of TNBS-induced intestinal inflammation in crucian carp (Carassius carassius) by oral administration of bioactive Bioactive food derived peptides . | FISH & SHELLFISH IMMUNOLOGY , 2022 , 131 , 999-1005 . |
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Tubeimoside I (TBMS) is a natural compound with antitumor properties. However, the detailed mechanism underlying the function of TBMS in liver cancer has not been fully elucidated. In the present study, TBMS was shown to suppress cell proliferation and induce S phase cell cycle arrest in HepG2 cells. Furthermore, TBMS treatment induced autophagy, evidenced by autophagosome accumulation, and increased the mRNA expression of Beclin 1 and microtubule-associated protein 1 light chain 3 (LC3)-I. However, flow cytometry analysis demonstrated that TBMS exerted no effect on cell apoptosis. Moreover, TBMS increased the phosphorylation of AMP-activated protein kinase (AMPK) in a concentration-dependent manner, thereby activating the AMPK signaling pathway. A specific AMPK inhibitor, compound C (CC), caused markedly suppressed TBMS-induced accumulation of LC3-II. In addition, the mRNA expression of LC3-I and Beclin 1 was also suppressed in cells treated with TBMS and CC in combination. The results of the present study provide new insights into the role of TBMS in inducing autophagy and support the potential application of TBMS for liver cancer treatment in the clinical setting.
Keyword ：
AMP-activated protein kinase AMP-activated protein kinase autophagy autophagy liver cancer liver cancer tubeimoside I tubeimoside I
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| GB/T 7714 | Ruan, Chengxu , You, Lijuan , Qiu, Yingdong et al. Tubeimoside I induces autophagy in HepG2 cells by activating the AMP-activated protein kinase signaling pathway [J]. | ONCOLOGY LETTERS , 2020 , 20 (1) : 623-630 . |
| MLA | Ruan, Chengxu et al. "Tubeimoside I induces autophagy in HepG2 cells by activating the AMP-activated protein kinase signaling pathway" . | ONCOLOGY LETTERS 20 . 1 (2020) : 623-630 . |
| APA | Ruan, Chengxu , You, Lijuan , Qiu, Yingdong , Cui, Xiao , Wu, Defeng . Tubeimoside I induces autophagy in HepG2 cells by activating the AMP-activated protein kinase signaling pathway . | ONCOLOGY LETTERS , 2020 , 20 (1) , 623-630 . |
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Lutein is a metabolite produced by plants or microalgae, and offers various health benefits. Chlorella sorokiniana FZU60 is considered a hyper-lutein producing microalga. In this study, the effects of temperature and light intensity on cell growth and lutein accumulation by FZU60 under mixotrophic conditions were investigated. Maximal lutein productivity and production were obtained at 33 degrees C, and high light intensity enhanced cell concentration but decreased lutein content when nitrate was replete. Further, two fed-batch strategies with gradient (fed-batch strategy I) or constant (fed-batch strategy II) NaAc feeding concentrations were compared. Results showed that the second strategy was more desirable for lutein production. Moreover, two types of mixotrophy/photoautotrophy two-stage strategies with a light intensity of 750 mu mol/m(2)/s (two-stage strategy I) or 150 mu mol/m(2)/s (two-stage strategy II) in stage 2 were explored. Under the two-stage strategy I, high lutein content (11.22 mg/g), productivity (8.25 mg/L/d), and production (65.96 mg/L) were achieved. The lutein content, productivity, and production obtained in this study are higher than those reported in most related studies, indicating that the proposed strategy can be used in commercial production.
Keyword ：
Chlorella sorokiniana Chlorella sorokiniana Environmental conditions Environmental conditions Fed-batch strategy Fed-batch strategy Lutein Lutein Two-stage strategy Two-stage strategy
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| GB/T 7714 | Ma, Ruijuan , Zhang, Zhen , Ho, Shih-Hsin et al. Two-stage bioprocess for hyper-production of lutein from microalga Chlorella sorokiniana FZU60: Effects of temperature, light intensity, and operation strategies [J]. | ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS , 2020 , 52 . |
| MLA | Ma, Ruijuan et al. "Two-stage bioprocess for hyper-production of lutein from microalga Chlorella sorokiniana FZU60: Effects of temperature, light intensity, and operation strategies" . | ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 52 (2020) . |
| APA | Ma, Ruijuan , Zhang, Zhen , Ho, Shih-Hsin , Ruan, Chengxu , Li, Jun , Xie, Youping et al. Two-stage bioprocess for hyper-production of lutein from microalga Chlorella sorokiniana FZU60: Effects of temperature, light intensity, and operation strategies . | ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS , 2020 , 52 . |
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以分离纯化自冰鲜大黄鱼的3株希瓦氏菌(MA1-5、MA1-7、MA1-13)和3株假单胞菌(R3-1、R3-2、R3-5)为供试菌株,研究鱼源腐败菌的表面疏水性、自凝聚能力、生物膜和腐败菌对鱼体(鱼肠、鱼鳃和体表)黏液的黏附能力,探明黏附特性与不同部位黏附能力的相关性,并进一步研究生物膜在不同因素下的形成特性。研究表明,希瓦氏菌具有更强的表面疏水性和自凝聚能力,对鱼体黏液的黏附性强,并具有更强的生物膜形成能力。主成分分析和聚类分析说明黏附能力在属间存在差异性,属内具有相似性。腐败菌对鱼肠和鱼鳃的黏附能力与自凝聚能力和生物膜形成能力具有较高的相关性,对体表黏附能力只与生物膜形成能力有关。腐败菌生...
Keyword ：
假单胞菌 假单胞菌 大黄鱼 大黄鱼 希瓦氏菌 希瓦氏菌 生物膜 生物膜 黏附特性 黏附特性
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| GB/T 7714 | 张雯 , 卞丹 , 阮成旭 et al. 大黄鱼源腐败菌的黏附特性与生物膜特性分析 [J]. | 食品科学 , 2019 , 40 (14) : 84-90 . |
| MLA | 张雯 et al. "大黄鱼源腐败菌的黏附特性与生物膜特性分析" . | 食品科学 40 . 14 (2019) : 84-90 . |
| APA | 张雯 , 卞丹 , 阮成旭 , 时祥柱 , 倪莉 . 大黄鱼源腐败菌的黏附特性与生物膜特性分析 . | 食品科学 , 2019 , 40 (14) , 84-90 . |
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采用营养液水培方式,研究5 mg·L-1镉(Cd2+)对青萍生长、光合色素、渗透调节物质含量、抗氧化酶活性和细胞膜脂过氧化反应的影响及外施不同质量浓度脱落酸(ABA)对镉胁迫下青萍的缓解效应.结果表明:与空白组(CK)相比,镉能显著抑制青萍的生长,减少光合色素含量,降低超氧化物酶(SOD)活性,增加可溶性蛋白、可溶性糖和脯氨酸的含量,提高过氧化物酶(POD)和过氧化氢酶(CAT)活性及丙二醛(MDA)含量.ABA的叶面喷施能促进青萍的生长,随着ABA质量浓度的增大,青萍生长指标呈先上升后下降的趋势,另外,ABA的添加降低了青萍POD活性及MDA含量,并在质量浓度为1×10-4 mg·L-1或1×10-3 mg·L-1时可回到CK水平,为本试验的最佳处理质量浓度.
Keyword ：
缓解效应 缓解效应 脱落酸 脱落酸 镉胁迫 镉胁迫 青萍 青萍
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| GB/T 7714 | 游丽娟 , 阮成旭 , 袁重桂 et al. 外施脱落酸对镉胁迫下青萍生理特性的影响 [J]. | 福州大学学报（自然科学版） , 2019 , 47 (2) : 272-278 . |
| MLA | 游丽娟 et al. "外施脱落酸对镉胁迫下青萍生理特性的影响" . | 福州大学学报（自然科学版） 47 . 2 (2019) : 272-278 . |
| APA | 游丽娟 , 阮成旭 , 袁重桂 , 高俊晖 , 丘英栋 . 外施脱落酸对镉胁迫下青萍生理特性的影响 . | 福州大学学报（自然科学版） , 2019 , 47 (2) , 272-278 . |
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以分离纯化自冰鲜大黄鱼的3株希瓦氏菌(MA1-5、MA1-7、MA1-13)和3株假单胞菌(R3-1、R3-2、R3-5)为供试菌株,研究鱼源腐败菌的表面疏水性、自凝聚能力、生物膜和腐败菌对鱼体(鱼肠、鱼鳃和体表)黏液的黏附能力,探明黏附特性与不同部位黏附能力的相关性,并进一步研究生物膜在不同因素下的形成特性.研究表明,希瓦氏菌具有更强的表面疏水性和自凝聚能力,对鱼体黏液的黏附性强,并具有更强的生物膜形成能力.主成分分析和聚类分析说明黏附能力在属间存在差异性,属内具有相似性.腐败菌对鱼肠和鱼鳃的黏附能力与自凝聚能力和生物膜形成能力具有较高的相关性,对体表黏附能力只与生物膜形成能力有关.腐败菌生物膜的形成受初始菌浓度、培养时间、温度、pH值、盐含量等多种环境因素影响.希瓦氏菌在初始菌浓度106 CFU/mL以上、37℃、pH 7～8、0.8％NaCl时形成的生物膜量最多;生物膜在12～24 h期间快速形成,并在36 h达到峰值后,随培养时间延长而逐渐下降.经鱼体黏液包被后,生物膜形成量受到显著影响,鱼鳃黏液促进生物膜形成,鱼肠黏液抑制生物膜形成.
Keyword ：
假单胞菌 假单胞菌 大黄鱼 大黄鱼 希瓦氏菌 希瓦氏菌 生物膜 生物膜 黏附特性 黏附特性
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| GB/T 7714 | 张雯 , 卞丹 , 阮成旭 et al. 大黄鱼源腐败菌的黏附特性与生物膜特性分析 [J]. | 食品科学 , 2019 , 40 (14) : 84-90 . |
| MLA | 张雯 et al. "大黄鱼源腐败菌的黏附特性与生物膜特性分析" . | 食品科学 40 . 14 (2019) : 84-90 . |
| APA | 张雯 , 卞丹 , 阮成旭 , 时祥柱 , 倪莉 . 大黄鱼源腐败菌的黏附特性与生物膜特性分析 . | 食品科学 , 2019 , 40 (14) , 84-90 . |
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Bioengineered marine microalgae Schizochytrium sp. is currently used to produce docosahexaenoic acid (DHA). However, following DHA extraction, the remaining protein-rich materials are not well utilized. In this study, we report that marine microalgae bioengineered Schizochytrium sp. hydrolysate (MESH), which exhibits a unique peptide profile as identified by Ultra Performance Liquid Chromatography coupled with Q-TOF mass spectrometry(UPLC/Q-TOF-MS), ameliorated bowel inflammation in mice. In a mouse model of experimentalcolitis induced by dextran sulfate sodium, compared with the control mice, the mice treated with MESH were highly resistant to colitis, as demonstrated by marked reductions in body weight loss, clinical colitis scores, colonic histological damage, and colonic inflammation. Mechanistically, MESH attenuated the induction of pro-inflammatory cytokines and increased the induction of anti-inflammatory cytokines. MESH also promoted the proliferation of colonic crypt stem cells and progenitor cells required for crypt repair. Collectively, these results reveal a previously unrecognized role of MESH as a potential anti-inflammatory treatment for colitis.
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| GB/T 7714 | Wang, Xiaoli , Wang, Heng , Pierre, Joseph F. et al. Marine microalgae bioengineered Schizochytrium sp. meal hydrolysates inhibits acute inflammation [J]. | SCIENTIFIC REPORTS , 2018 , 8 . |
| MLA | Wang, Xiaoli et al. "Marine microalgae bioengineered Schizochytrium sp. meal hydrolysates inhibits acute inflammation" . | SCIENTIFIC REPORTS 8 (2018) . |
| APA | Wang, Xiaoli , Wang, Heng , Pierre, Joseph F. , Wang, Sheng , Huang, Huifang , Zhang, Jun et al. Marine microalgae bioengineered Schizochytrium sp. meal hydrolysates inhibits acute inflammation . | SCIENTIFIC REPORTS , 2018 , 8 . |
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采用单因子梯度试验法,设定20(I)、28(II)和40 kHz(III)3个不同低频率超声波试验组,以不进行超声波作用为对照组,研究超声波对养殖水体中浮游植物群落结构的影响.40d的试验结果表明:对照组浮游植物的丰度为各组最高,试验组II、III中,浮游植物丰度分别为3.8×105、2.3×105 ind·L-1,显著低于对照组(P＜0.05).共鉴定到浮游植物种类属数为16个,数目大小依次为试验组Ⅰ＞试验组Ⅱ＞试验组Ⅲ＞对照组.试验组Ⅰ中Shannonwiener多样性指数(日)、Margalef丰富度指数(D)分别达1.661±0.055和1.071±0.020,为各组最高;试验组Ⅲ中Pielou均匀度指数(J)达0.619±0.018,为各组最高;与对照组相比,施加20 kHz超声波显著提高了养殖水体中浮游植物生物群落多样性(P＜0.05).
Keyword ：
低频超声波 低频超声波 养殖水体 养殖水体 浮游植物 浮游植物 生物多样性 生物多样性 群落结构 群落结构
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| GB/T 7714 | 高俊晖 , 阮成旭 , 袁重桂 et al. 不同频率超声波对养殖水体浮游植物群落结构的影响 [J]. | 福州大学学报（自然科学版） , 2018 , 46 (5) : 748-752 . |
| MLA | 高俊晖 et al. "不同频率超声波对养殖水体浮游植物群落结构的影响" . | 福州大学学报（自然科学版） 46 . 5 (2018) : 748-752 . |
| APA | 高俊晖 , 阮成旭 , 袁重桂 , 游丽娟 , 崔笑 . 不同频率超声波对养殖水体浮游植物群落结构的影响 . | 福州大学学报（自然科学版） , 2018 , 46 (5) , 748-752 . |
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对工厂化养殖模式、网箱养殖模式养殖的大黄鱼和野生大黄鱼进行了肌肉常规营养成分、氨基酸和脂肪酸的分析比较.研究结果表明,工厂化养殖模式和网箱养殖模式的大黄鱼粗蛋白含量均显著低于野生大黄鱼(P<0.05),粗脂肪含量均显著高于野生大黄鱼(P<0.05).工厂化养殖模式的大黄鱼必需氨基酸总量、呈味氨基酸总量和氨基酸总量均显著高于网箱养殖大黄鱼,但显著低于野生大黄鱼(P<0.05).工厂化养殖模式的大黄鱼多不饱和脂肪酸以及二十碳五烯酸+二十二碳六烯酸均显著高于网箱养殖的而显著低于野生大黄鱼(P<0.05).工厂化养殖模式养殖大黄鱼可以生产出肉质营养结构和风味优于传统网箱养殖的大黄鱼.
Keyword ：
大黄鱼 大黄鱼 工厂化养殖模式 工厂化养殖模式 比较分析 比较分析 肉质 肉质
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| GB/T 7714 | 阮成旭 , 袁重桂 , 陶翠丽 et al. 不同养殖模式对大黄鱼肉质的影响 [J]. | 水产科学 , 2017 , 36 (5) : 623-627 . |
| MLA | 阮成旭 et al. "不同养殖模式对大黄鱼肉质的影响" . | 水产科学 36 . 5 (2017) : 623-627 . |
| APA | 阮成旭 , 袁重桂 , 陶翠丽 , 陈强 , 林文相 , 李昊 . 不同养殖模式对大黄鱼肉质的影响 . | 水产科学 , 2017 , 36 (5) , 623-627 . |
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用单因素分析法研究赤霉素(GA)、6-苄基氨基嘌呤(6-BA)和吲哚乙酸(IAA)3种植物激素对长茎葡萄蕨藻生长、分枝数及叶绿素、粗多糖含量的影响.结果表明:①0.8、1.4 mg·L-1的6-BA使长茎葡萄蕨藻质量相对增加率和比生长速率达到最大,11 mg·L-1为GA的最适使用浓度;IAA对长茎葡萄蕨藻质量相对增加率和比生长速率无显著影响;②GA、6-BA、IAA的最适促分枝浓度分别为14.0、0.4、5.0 mg·L-1;③ 不同浓度GA对长茎葡萄蕨藻叶绿素含量和粗多糖含量影响不显著,不同浓度IAA对其叶绿素含量的影响不显著,但对其粗多糖含量影响显著.
Keyword ：
分枝数 分枝数 叶绿素 叶绿素 植物激素 植物激素 粗多糖 粗多糖 长茎葡萄蕨藻 长茎葡萄蕨藻
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| GB/T 7714 | 陶翠丽 , 袁重桂 , 阮成旭 et al. 不同植物激素对长茎葡萄蕨藻生长的影响 [J]. | 福州大学学报（自然科学版） , 2017 , 45 (2) : 291-295 . |
| MLA | 陶翠丽 et al. "不同植物激素对长茎葡萄蕨藻生长的影响" . | 福州大学学报（自然科学版） 45 . 2 (2017) : 291-295 . |
| APA | 陶翠丽 , 袁重桂 , 阮成旭 , 陈强 , 林文相 . 不同植物激素对长茎葡萄蕨藻生长的影响 . | 福州大学学报（自然科学版） , 2017 , 45 (2) , 291-295 . |
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