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学者姓名：徐两军
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Abstract ：
Saxitoxin (STX) has high toxicity, and is water soluble, acid stable and thermostable. Therefore, STX in seawater can be accumulated by marine organism to form bioaccumulation. To ensure the safety of seafood for consumption, it is crucial to accurately determine trace STX in seawater and seafood. We herein developed a novel magnetic electrochemical immunosensor for ultra-sensitive detection of STX in seawater and seafood by using non-competitive strategy. The immunosensor employs STX-specific antibody-functionalized magnetic beads (MBs) for STX recognition, palladium-doped graphitic carbon nitride (g-C3N4-PdNPs) peroxidase mimetic for catalyzing H2O2-mediated oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) to generate signal. The immunosensor combines the merits of g-C3N4-PdNPs peroxidase mimetic, non-competitive strategy, MBs-based antibody recognition and magnetic gold electrode, and thus has excellent stability, lower cost, no risk of false positive result, high sensitivity and strong ability resist to matrix interference. The proposed immunosensor has been successfully used to detect trace STX in seawater and shellfish samples with a detection limit of 1.2 pg/mL (4.0 x 10(-12) M), a recovery of 93-107% and a relative standard deviation (RSD, n = 5) < 5%. The success of this study provided a promising approach for the rapid and on-site detection of trace STX in seawater and seafood.
Keyword ：
Enzyme mimetics Enzyme mimetics Graphitic carbon nitride Graphitic carbon nitride Seafood Seafood Seawater Seawater Shellfish Shellfish Shellfish toxins Shellfish toxins
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| GB/T 7714 | Jin, Xin , Chen, Jianlang , Zeng, Xiaoxue et al. A signal-on magnetic electrochemical immunosensor for ultra-sensitive detection of saxitoxin using palladium-doped graphitic carbon nitride-based non-competitive strategy [J]. | BIOSENSORS & BIOELECTRONICS , 2019 , 128 : 45-51 . |
| MLA | Jin, Xin et al. "A signal-on magnetic electrochemical immunosensor for ultra-sensitive detection of saxitoxin using palladium-doped graphitic carbon nitride-based non-competitive strategy" . | BIOSENSORS & BIOELECTRONICS 128 (2019) : 45-51 . |
| APA | Jin, Xin , Chen, Jianlang , Zeng, Xiaoxue , Xu, LiangJun , Wu, Yongning , Fu, FengFu . A signal-on magnetic electrochemical immunosensor for ultra-sensitive detection of saxitoxin using palladium-doped graphitic carbon nitride-based non-competitive strategy . | BIOSENSORS & BIOELECTRONICS , 2019 , 128 , 45-51 . |
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A novel tumor-targeting and release-controllable drug delivery nano-platform with persistent luminescence was constructed for imaging-guided cancer chemotherapy. The persistent luminescent drug delivery nano-platform employed the persistent luminescent nanoparticles ZnGa2O4:Cr3+,Sn4+ (ZGCS) to realize the repeatable luminescence imaging of cells or tissues with high signal-to-noise ratio. Mesoporous silica (MS) was coated on the ZGCS surface to load drugs, and hyaluronic acid (HA) acted as gatekeeper and targeting moiety. The proposed nano-platform could repeatedly be excited to produce persistent luminescence under red light (550 nm) irradiation to realize repeatable luminescence imaging of cells or tissues without in situ excitation, and simultaneously possessed an excellent tumor-targeting ability towards CD-44 over-expressing cancer cells and controllable drug release with hyaluronic acidase (Hyal-1) response. By using the nano-platform, we have successfully delivered the non-fluorescent chemotherapeutic drug, paclitaxel (Ptx), into human breast adenocarcinoma cells (MCF-7 cells) with a loading capacity of 0.187 mg mg(-1) and Hyal-1 responsive controllable-release, and simultaneously, we monitored the distribution of the nano-platform and the release of Ptx in target cells by luminescence-imaging. The combination of excellent persistent luminescence feature, tumor-targeting ability and controllable drug release makes this nano-platform a promising tool for imaging-guided cancer chemotherapy, which will greatly enhance the chemotherapeutic efficiency of the drug and reduce the side effects.
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| GB/T 7714 | Jiang, Wenjing , Huang, Lin , Mo, Fan et al. Persistent luminescent multifunctional drug delivery nano-platform based on nanomaterial ZnGa2O4:Cr3+,Sn4+ for imaging-guided cancer chemotherapy [J]. | JOURNAL OF MATERIALS CHEMISTRY B , 2019 , 7 (18) : 3019-3026 . |
| MLA | Jiang, Wenjing et al. "Persistent luminescent multifunctional drug delivery nano-platform based on nanomaterial ZnGa2O4:Cr3+,Sn4+ for imaging-guided cancer chemotherapy" . | JOURNAL OF MATERIALS CHEMISTRY B 7 . 18 (2019) : 3019-3026 . |
| APA | Jiang, Wenjing , Huang, Lin , Mo, Fan , Zhong, Yingying , Xu, Liangjun , Fu, FengFu . Persistent luminescent multifunctional drug delivery nano-platform based on nanomaterial ZnGa2O4:Cr3+,Sn4+ for imaging-guided cancer chemotherapy . | JOURNAL OF MATERIALS CHEMISTRY B , 2019 , 7 (18) , 3019-3026 . |
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An exonuclease-assisted multicolor aptasensor was developed for the visual detection of ochratoxin A (OTA). It is based on the etching of gold nanorods (AuNRs) mediated by a G-quadruplex-hemin DNAzyme. A DNA sequence (AG4-OTA) was designed that comprises a hemin aptamer and an OTA aptamer. OTA binds to AG4-OTA to form an antiparallel G-quadruplex, which halts its digestion by exonuclease I (Exo I) from the 3'-end of AG4-OTA. Thus, the retained hemin aptamer can bind to hemin to form a G-quadruplex-hemin DNAzyme. This DNAzyme has peroxidase-like activity that catalyzes the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) by H2O2 to produce its diimine derivative (TMB2+) in acidic solution. TMB2+ can etch the AuNRs by oxidizing Au(0) into Au(I). This results in the generation of rainbow-like colors and provides a multicolor platform for the visual detection of OTA. The assay is based on the use of a single isolated aptamer and possesses obvious advantages such as multi-color visual inspection, relatively high sensitivity and accuracy. It can be used to detect as little as 30 nM concentrations of OTA by visual observation and even 10 nM concentrations by spectrophotometry. The method was successfully applied to the determination of OTA in spiked beer where it gave recoveries of 101-108%, with a relative standard deviation (RSD, n = 5) of <5%.
Keyword ：
Aptamer Aptamer Beer Beer Biosensor Biosensor Biotoxin Biotoxin Colorimetric detection Colorimetric detection DNA DNA Gold nanorods Gold nanorods Mycotoxins Mycotoxins
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| GB/T 7714 | Yu, Xinhui , Lin, Yaohui , Wang, Xusheng et al. Exonuclease-assisted multicolor aptasensor for visual detection of ochratoxin A based on G-quadruplex-hemin DNAzyme-mediated etching of gold nanorod [J]. | MICROCHIMICA ACTA , 2018 , 185 (5) . |
| MLA | Yu, Xinhui et al. "Exonuclease-assisted multicolor aptasensor for visual detection of ochratoxin A based on G-quadruplex-hemin DNAzyme-mediated etching of gold nanorod" . | MICROCHIMICA ACTA 185 . 5 (2018) . |
| APA | Yu, Xinhui , Lin, Yaohui , Wang, Xusheng , Xu, Liangjun , Wang, Zongwen , Fu, FengFu . Exonuclease-assisted multicolor aptasensor for visual detection of ochratoxin A based on G-quadruplex-hemin DNAzyme-mediated etching of gold nanorod . | MICROCHIMICA ACTA , 2018 , 185 (5) . |
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Assessing the polymerization efficiency of a dual-cured resin cement through zirconia with three different cusp inclinations is the aim of this study. Seventy-two resin cement (Multilink Speed) specimens were light-cured through the zirconia with three different cusp inclinations (30 degrees, 20 degrees, and 0 degrees) for 20 s or 40 s (n = 12). For each group, the Vickers hardness (VH) measurement was performed on half of the specimens, whereas the degree of conversion (DC) was tested for the other half with a Fourier transform infrared spectrometer. The recorded data were analyzed with a two-way ANOVA. Varied VH (32.3 +/- 3.2-40.3 +/- 4.8) and DC (50.5% +/- 3.0%-59.6% +/- 2.4%) values were obtained from different groups. The cusp inclinations significantly affect the VH (p = 0.001) and DC (p < 0.001) of the resin cement, and the curing time exhibited a significant effect on the DC (p = 0.014) of resin cement but not on the VH (p = 0.167). In conclusion, the cusp inclination of zirconia significantly affects the polymerization efficiency of dual-cured resin cement.
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| GB/T 7714 | Zhang, Chang-Yuan , Yu, Hao , Cheng, Yi-Ling et al. Polymerization Efficiency of a Dual-Cured Resin Cement through Zirconia with Three Different Cusp Inclinations [J]. | JOURNAL OF SPECTROSCOPY , 2018 , 2018 . |
| MLA | Zhang, Chang-Yuan et al. "Polymerization Efficiency of a Dual-Cured Resin Cement through Zirconia with Three Different Cusp Inclinations" . | JOURNAL OF SPECTROSCOPY 2018 (2018) . |
| APA | Zhang, Chang-Yuan , Yu, Hao , Cheng, Yi-Ling , Wu, Wen-Zhou , Xu, Liang-Jun , Cheng, Hui . Polymerization Efficiency of a Dual-Cured Resin Cement through Zirconia with Three Different Cusp Inclinations . | JOURNAL OF SPECTROSCOPY , 2018 , 2018 . |
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In this research, a novel tumor targeting dual stimuli responsive nanoplatform was fabricated for the controllable delivery and release of a drug to realize chemo-photothermal synergetic cancer therapy by integrating a DNA aptamer with polydopamine reduced graphene oxide (rGO-PDA) nanosheets. The rGO-PDA nanosheets simultaneously acted as a near-infrared radiation (NIR) photothermal agent to generate hyperthermia for photothermal therapy and a nano-carrier for loading doxorubicin (DOX), and the specially designed DNA aptamer served as a supplementary carrier for DOX loading as well as targeting moiety/gatekeeper for specific cellular recognition and controllable release of DOX. The proposed nanoplatform possessed a good targeting ability, remarkable photothermal conversion ability and intelligent drug release with both pH and photothermal heating dual stimuli response. The nanoplatform was successfully used to selectively deliver DOX to protein tyrosine kinase 7 over-expressing cancer cells with a loading capacity of 1.56 mg mg(-1) and controllable drug release, which responded to both acidic intracellular environments and NIR irradiation. The combination of the dual stimuli responsive controllable release and the dual nanocarrier for drug loading results in efficient chemo-photothermal synergetic therapy and holds great potential for multimodal cancer therapy.
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| GB/T 7714 | Jiang, Wenjing , Mo, Fan , Lin, Yaohui et al. Tumor targeting dual stimuli responsive controllable release nanoplatform based on DNA-conjugated reduced graphene oxide for chemo-photothermal synergetic cancer therapy [J]. | JOURNAL OF MATERIALS CHEMISTRY B , 2018 , 6 (26) : 4360-4367 . |
| MLA | Jiang, Wenjing et al. "Tumor targeting dual stimuli responsive controllable release nanoplatform based on DNA-conjugated reduced graphene oxide for chemo-photothermal synergetic cancer therapy" . | JOURNAL OF MATERIALS CHEMISTRY B 6 . 26 (2018) : 4360-4367 . |
| APA | Jiang, Wenjing , Mo, Fan , Lin, Yaohui , Wang, Xusheng , Xu, LiangJun , Fu, FengFu . Tumor targeting dual stimuli responsive controllable release nanoplatform based on DNA-conjugated reduced graphene oxide for chemo-photothermal synergetic cancer therapy . | JOURNAL OF MATERIALS CHEMISTRY B , 2018 , 6 (26) , 4360-4367 . |
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Herein, a novel palladium-doped graphitic carbon nitride nanosheet (g-C3N4-PdNPs) is reported. The prepared g-C3N4-PdNPs has a 6.7 and 14.0 times higher peroxidase-like activity in comparison with pure Pd nanoparticles (PdNPs) and graphitic carbon nitride (g-C3N4) nanosheets respectively, and can be stably stored for 3 months. The high peroxidase-like activity make g-C3N4-PdNPs effectively catalyze H2O2-mediation oxidization of 3,3,5,5-tetramethylbenzidine to generate a color change from colorless to blue under lower concentration level and shorter time. The g-C3N4-PdNPs can be used as peroxidase mimetic to develop sensitive and specific colorimetric method for the rapid detection of glucose in the serum, and to fabricate a simple and cheap portable test kit for instrument-free visual detection of glucose in serum. The portable test kit possesses obvious advantages such as low-cost, short detection time, tiny sample consumption, excellent specificity, and higher visual sensitivity. The visual detection limit of portable test kit is lower than the glucose concentration in the serum of a diabetic. Using portable test kit, the glucose in serum can be visually detected by bare eye observation within 30 min with only 30 mu L serum consumption. The success of this study provided a potential approach for low-cost and instrument-free see diabetes in clinical early diagnosis.
Keyword ：
enzyme mimetics enzyme mimetics glucose glucose graphite phase carbon nitride graphite phase carbon nitride horseradish peroxidase horseradish peroxidase nanomaterials enzyme mimetics nanomaterials enzyme mimetics
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| GB/T 7714 | Jin, Xin , Zhong, Yingying , Chen, Lian et al. A Palladium-Doped Graphitic Carbon Nitride Nanosheet with High Peroxidase-Like Activity: Preparation, Characterization, and Application in Glucose Detection [J]. | PARTICLE & PARTICLE SYSTEMS CHARACTERIZATION , 2018 , 35 (3) . |
| MLA | Jin, Xin et al. "A Palladium-Doped Graphitic Carbon Nitride Nanosheet with High Peroxidase-Like Activity: Preparation, Characterization, and Application in Glucose Detection" . | PARTICLE & PARTICLE SYSTEMS CHARACTERIZATION 35 . 3 (2018) . |
| APA | Jin, Xin , Zhong, Yingying , Chen, Lian , Xu, Liangjun , Wu, Yongning , Fu, FengFu . A Palladium-Doped Graphitic Carbon Nitride Nanosheet with High Peroxidase-Like Activity: Preparation, Characterization, and Application in Glucose Detection . | PARTICLE & PARTICLE SYSTEMS CHARACTERIZATION , 2018 , 35 (3) . |
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As one of paralytic shellfish toxins, the saxitoxin (STX) in the aqueous environment can be accumulated by most shellfish, and thus harms human health through the food chain. Therefore, it is crucial to determine trace STX in seafood samples in order to ensure the safety of seafood consumption. In this study, we developed a novel indirect method for ultrasensitively determining trace STX in seafood by using CE-ICP-MS together with Eu3+ chelate labeling. We demonstrated that diethylenetriamine-N,N,N',N'', N''-pentaacetic acid (DTPA) can couple with STX and simultaneously chelate with Eu3+ to realize metallic labeling of STX, and thus realize the ultrasensitive quantification of trace STX with CEICP-MS. The proposed method has strong antiinterference ability, good stability, and extremely high sensitivity. It could be used to determine trace STX in seafood samples with an extremely low detection limit of 0.38 fmol (3.8x10(-9) M, 100 nL sample injection) and a relative standard deviation (RSD, n = 5) < 7%. The success of this study provides an alternative to precise quantification of ultra-trace STX in seafood samples, and further expands the application of ICP-MS.
Keyword ：
CE CE ICP-MS ICP-MS Paralytic shellfish toxins Paralytic shellfish toxins Saxitoxin Saxitoxin Seafood Seafood
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| GB/T 7714 | He, Ye , Mo, Fan , Chen, Danlong et al. Capillary electrophoresis inductively coupled plasma mass spectrometry combined with metal tag for ultrasensitively determining trace saxitoxin in seafood [J]. | ELECTROPHORESIS , 2017 , 38 (3-4) : 469-476 . |
| MLA | He, Ye et al. "Capillary electrophoresis inductively coupled plasma mass spectrometry combined with metal tag for ultrasensitively determining trace saxitoxin in seafood" . | ELECTROPHORESIS 38 . 3-4 (2017) : 469-476 . |
| APA | He, Ye , Mo, Fan , Chen, Danlong , Xu, LiangJun , Wu, Yongning , Fu, FengFu . Capillary electrophoresis inductively coupled plasma mass spectrometry combined with metal tag for ultrasensitively determining trace saxitoxin in seafood . | ELECTROPHORESIS , 2017 , 38 (3-4) , 469-476 . |
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For rapidly and sensitively determining ultra-trace methyl mercury (MeHg) in aqueous environment, we herein synthesized a MeHg ion-imprinted magnetic nanoparticle (MeHg IIMN) to simply and specifically extract/concentrate ultra-trace MeHg from water samples. The MeHg IIMN employed core-shell Fe3O4@SiO2 nanoparticles (Fe3O4@SiO2 NPs), as supporting structure, the complex ion of 1-pyrrolidinecarbodithioic acid and MeHg (PDC-CH3Hg+) as template, methacrylic acid (MM) as functional monomer and trimethylolpropane trimethacrylate (TMPTM) as cross linker. The MeHg IIMN offered obvious advantages such as low cost, easy manipulation, better specificity and stability, and recycling characteristics. It can be used to separate/concentrate ultra-trace MeHg from natural water sample within 30 min with a recovery > 95%, an enrichment factor of 250, a relative standard deviation (RSD, n = 5) < 7%, a 25 mg MeHg/g of maximum adsorption capacity, 50 times of recycling, and without obvious interference of other ions. Combining with capillary electrophoresis-inductively coupled plasma mass spectrometry (CE-ICP-MS), it can be used for the accurate detection of ultra-trace methyl mercury in natural water samples with a limit of detection of 0.084 pg/mL, a recovery of 92-99% and a RSD (n = 5) < 8%. The success of this study promises a valuable technique for relatively simple detection of ultra-trace methyl mercury in aqueous environment. (C) 2017 Elsevier B.V. All rights reserved.
Keyword ：
Ion imprinted polymer Ion imprinted polymer Magnetic nano-particle Magnetic nano-particle Mercury Mercury Methyl mercury Methyl mercury Speciation analysis Speciation analysis
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| GB/T 7714 | Jiang, Wenjing , Jin, Xin , Yu, Xinhui et al. Ion-imprinted magnetic nanoparticles for specific separation and concentration of ultra-trace methyl mercury from aqueous sample [J]. | JOURNAL OF CHROMATOGRAPHY A , 2017 , 1496 : 167-173 . |
| MLA | Jiang, Wenjing et al. "Ion-imprinted magnetic nanoparticles for specific separation and concentration of ultra-trace methyl mercury from aqueous sample" . | JOURNAL OF CHROMATOGRAPHY A 1496 (2017) : 167-173 . |
| APA | Jiang, Wenjing , Jin, Xin , Yu, Xinhui , Wu, Weihua , Xu, LiangJun , Fu, FengFu . Ion-imprinted magnetic nanoparticles for specific separation and concentration of ultra-trace methyl mercury from aqueous sample . | JOURNAL OF CHROMATOGRAPHY A , 2017 , 1496 , 167-173 . |
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A novel colorimetric biosensor, which employs DNAzyme-functionalized magnetic beads (MBs) as recognition probe, enzyme-assisted catalytic oxidation of TMB (3,3',5,5'-tetramethylbenzidine sulfate) as signal and DNA hybridization chain reaction as amplification strategy, has been developed for detecting trace uranyl ion (UO22+ in seafood and aqueous environment with high sensitivity and specificity. We demonstrated that UO22+ can specifically cleave DNAzyme immobilized on MBs surface to release a short single-strand DNA (primer), and the released primer trigger DNA hybridization chain reaction to form a long one dimensional DNA concatamer on the MBs surface. The resulting long DNA concatamer could capture a large amount of HRP to generate the one UO22+-to-multiple HRP amplification effect. Upon the addition of TME-H2O2 solution, the HRP-tagged DNA concatamer-MBs conjugates could catalyze the H2O2-mediated oxidation of TMB, and thus results in a color change from colorless to blue in solution. This provided a sensitive and selective sensing platform for the visual or colorimetric detection of UO22+. The proposed biosensor has high sensitivity and strong anti-interference capability, it can be used to detect as low as 2.5 ppb (9.25 nM) of UO22+ by naked-eye observation and 0.09 ppb (0.33 nM) of UO22+ by UV-visible spectrometry with no interference of other ions and a RSD <= 6% (n = 5). With the help of this method, we have successfully determined trace UO22+ in fish muscle and river water with a recovery of 93 -106%. High sensitivity and specificity, as well operation convenience, low cost and strong resistibility to the matrix, which makes our method a potential approach for the on-site detection of UO22+ in seafood and aqueous environment. (C) 2016 Elsevier B.V. All rights reserved.
Keyword ：
Colorimetric biosensor Colorimetric biosensor DNA hybridization chain reaction DNA hybridization chain reaction DNAzyme DNAzyme Magnetic beads Magnetic beads Uranyl ion Uranyl ion
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| GB/T 7714 | Zhang, Hongyan , Cheng, Xian , Chen, Lian et al. Magnetic beads-based DNA hybridization chain reaction amplification and DNAzyme recognition for colorimetric detection of uranyl ion in seafood [J]. | ANALYTICA CHIMICA ACTA , 2017 , 956 : 63-69 . |
| MLA | Zhang, Hongyan et al. "Magnetic beads-based DNA hybridization chain reaction amplification and DNAzyme recognition for colorimetric detection of uranyl ion in seafood" . | ANALYTICA CHIMICA ACTA 956 (2017) : 63-69 . |
| APA | Zhang, Hongyan , Cheng, Xian , Chen, Lian , Mo, Fan , Xu, LiangJun , Fu, FengFu . Magnetic beads-based DNA hybridization chain reaction amplification and DNAzyme recognition for colorimetric detection of uranyl ion in seafood . | ANALYTICA CHIMICA ACTA , 2017 , 956 , 63-69 . |
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This study herein develops a novel tumor-targeting photothermal heating-responsive nanoplatform for enhanced photodynamic therapy (PDT). The nanoplatform is prepared by using dopamine-reduced graphene oxide nanosheets (rGO-PDA) to enhance photothermal ability, mesoporous silica coated on the rGO-PDA surface to load drug, and hyaluronic acid to act as gate keeper as well as targeting moiety. The developed nanoplatform has outstanding biocompatibility, well targeting ability, excellent near-infrared radiation (NIR) photothermal conversion ability, controllable drug release with NIR irradiation response, and the capacity of delivering diverse drugs. The proposed nanoplatform is successfully used to specifically deliver chlorin e6 (Ce6) to CD-44 over-expressing cancer cells for PDT with a loading capacity of 0.605 mg mg(-1) and controllable Ce6 release with NIR irradiation response. The combination of excellent NIR photothermal conversion ability and controllable Ce6 release confers the nanoplatform with enhanced singlet oxygen generation, which leads to more significant destruction of target cancer cells. The prepared photothermal heating-responsive nanoplatform can enhance photodynamic therapeutic efficiency and hold great potential for multimodal cancer therapy.
Keyword ：
cancer therapy cancer therapy graphene oxide graphene oxide hyaluronic acid hyaluronic acid mesoporous silica mesoporous silica photodynamic therapy photodynamic therapy
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| GB/T 7714 | Jiang, Wenjing , Mo, Fan , Jin, Xin et al. Tumor-Targeting Photothermal Heating-Responsive Nanoplatform Based on Reduced Graphene Oxide/Mesoporous Silica/Hyaluronic Acid Nanocomposite for Enhanced Photodynamic Therapy [J]. | ADVANCED MATERIALS INTERFACES , 2017 , 4 (20) . |
| MLA | Jiang, Wenjing et al. "Tumor-Targeting Photothermal Heating-Responsive Nanoplatform Based on Reduced Graphene Oxide/Mesoporous Silica/Hyaluronic Acid Nanocomposite for Enhanced Photodynamic Therapy" . | ADVANCED MATERIALS INTERFACES 4 . 20 (2017) . |
| APA | Jiang, Wenjing , Mo, Fan , Jin, Xin , Chen, Lian , Xu, LiangJun , Guo, Liangqia et al. Tumor-Targeting Photothermal Heating-Responsive Nanoplatform Based on Reduced Graphene Oxide/Mesoporous Silica/Hyaluronic Acid Nanocomposite for Enhanced Photodynamic Therapy . | ADVANCED MATERIALS INTERFACES , 2017 , 4 (20) . |
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