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学者姓名：付凤富
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Abstract ：
Alkaline phosphatase (ALP) is a vital protein catalyst that holds a central role in modulating protein metabolism and function throughout organisms, and serves as a reliable foundation for the treatment of human diseases. Thus, the development of a sensitive and selective detection strategy for ALP is considerable scientific and practical significance. In this study, SiO2 was chosen as the carrier for encapsulating Ru-1 luminophores to effectively prevent the leakage of Ru-1 molecules. Meanwhile, during the preparation process, the introduction of a poor solvent induces aggregation of Ru-1 molecules into nanoparticles, thereby enhancing emission through the aggregation-induced emission effect. Furthermore, using an in-situ growth method,we developed a phosphorescence-based detection strategy, which builds on Ru-SiO2-MnO2 nanocomposites. L-ascorbic acid (AA) can be generated by ALP, which catalyzes the hydrolysis of sodium L-ascorbyl-2-phosphate (AAP). This process further breaks down MnO2 and triggers the phosphorescence sensor through a "turn-on" mechanism. Moreover, by exploiting the capacity of Na3VO4 to suppress ALP activity, this phosphorescent method was further employed for the identification of ALP inhibitors. Under the optimized experimental conditions, the phosphorescent intensity displayed a linear association with ALP concentrations ranging from 0.05 to 17 U/L, while the limit of detection was as low as 0.031 U/L. The proposed strategy eliminates the need for complex washing or modification procedures and avoids the aggregation-caused quenching phenomenon of traditional organic dyes, thus standing out as an uncomplicated, economical "turn-on" phosphorescent detection method for ALP. This approach also demonstrated excellent applicability in the analysis of serum samples.
Keyword ：
Aggregation-induced emission Aggregation-induced emission Alkaline phosphatase Alkaline phosphatase Nanocomposites Nanocomposites Phosphorescence Phosphorescence Ru(II) complex Ru(II) complex
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| GB/T 7714 | Zheng, Qianghui , Zhang, Jinyuan , Li, Mei-Jin et al. Aggregation-induced emission Ru(II) complex nanomaterials: Phosphorescent determination of alkaline phosphatase activity and inhibitors [J]. | MICROCHEMICAL JOURNAL , 2025 , 218 . |
| MLA | Zheng, Qianghui et al. "Aggregation-induced emission Ru(II) complex nanomaterials: Phosphorescent determination of alkaline phosphatase activity and inhibitors" . | MICROCHEMICAL JOURNAL 218 (2025) . |
| APA | Zheng, Qianghui , Zhang, Jinyuan , Li, Mei-Jin , Fu, Fengfu . Aggregation-induced emission Ru(II) complex nanomaterials: Phosphorescent determination of alkaline phosphatase activity and inhibitors . | MICROCHEMICAL JOURNAL , 2025 , 218 . |
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A simple method for one-step synthesis of aggregated gold nanoparticles (a-AuNPs) using single-layer carbon dots (s-CDs) as the capping agents has been proposed. The obtained a-AuNPs are mainly composed of several spherical AuNPs of 20-25 nm sized, which aggregate to form nanogaps of similar to 1 nm. Furthermore, the obtained aAuNPs produce a strong localized surface plasmon resonance (LSPR) absorption band centered at around 640 nm, which is quite close to the wavelength of the commonly used 633 nm laser in surface enhanced Raman scattering (SERS). Thus, under the irradiation of 633 nm laser, a lot of electromagnetic field "hot spots" are formed at around the nanogaps, and strong SERS activity is achieved. The obtained a-AuNPs are dropped on tinfoil wafers to fabricate SERS substrates, which show the advantages of high sensitivity, fast response, good repeatability and satisfactory stability. On the basis, a sensitive SERS sensor is developed to detect malachite green in aquaculture water, with a low detection limit of 1 x 10(-9) mol/L.
Keyword ：
Aggregated gold nanoparticles Aggregated gold nanoparticles Localized surface plasmon resonance Localized surface plasmon resonance Malachite green Malachite green Plasmonic hotspots Plasmonic hotspots Surface enhanced Raman scattering Surface enhanced Raman scattering
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| GB/T 7714 | Zhou, Yongcong , Zhang, Jingwen , Lai, Xiaojing et al. Aggregated gold nanoparticles rich in electromagnetic field "hotspots " for surface enhanced Raman scattering [J]. | TALANTA , 2025 , 282 . |
| MLA | Zhou, Yongcong et al. "Aggregated gold nanoparticles rich in electromagnetic field "hotspots " for surface enhanced Raman scattering" . | TALANTA 282 (2025) . |
| APA | Zhou, Yongcong , Zhang, Jingwen , Lai, Xiaojing , Chen, Yiquan , Fu, Fengfu , Lin, Zhenyu et al. Aggregated gold nanoparticles rich in electromagnetic field "hotspots " for surface enhanced Raman scattering . | TALANTA , 2025 , 282 . |
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There are multiple lead species, including inorganic and organic lead, that may simultaneously be present in marine organisms. Thus, it is crucial to detect lead species in seafood for scientifically evaluating the health risk of lead in seafood. We herein developed a cost-effective short-column ion chromatography-inductively coupled plasma mass spectrometry (IC-ICP-MS) method and a broadly applicable microwave-assisted extraction method for the speciation analysis of lead in various types of seafood, including different species of seaweed, fish and shellfish. The short-column IC-ICP-MS method utilized low-cost cation guard columns as separation columns, offering high sensitivity, shorter analysis time and lower analysis costs. The microwave-assisted extraction method is widely applicable; it can be used to extract lead species from various types of seafood with an extraction efficiency >90%, while keeping lead species unaltered. In particular, the seafood extract obtained with the extraction method can be directly injected into the short-column IC-ICP-MS for the detection of lead species without extra pretreatment. The developed extraction and IC-ICP-MS method has been successfully used to detect Pb2+, tri-methyl lead (TML) and tri-ethyl lead (TEL) in various species of seaweed, fish and shellfish with a recovery of 94-105%, a relative standard deviation (RSD, n = 5) <6% and a method detection limit of 0.60-0.72 ng Pb g(-1) dried seafood. The success of this study offers a reliable and universal approach for objectively assessing the health risks associated with lead originating from seafood.
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| GB/T 7714 | Xu, Haochen , Zhu, Yiwei , Peng, Ying et al. A cost-effective method applicable to the speciation analysis of lead in various types of seafood based on short-column ion chromatography-inductively coupled plasma mass spectrometry [J]. | JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY , 2025 , 40 (5) : 1364-1372 . |
| MLA | Xu, Haochen et al. "A cost-effective method applicable to the speciation analysis of lead in various types of seafood based on short-column ion chromatography-inductively coupled plasma mass spectrometry" . | JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY 40 . 5 (2025) : 1364-1372 . |
| APA | Xu, Haochen , Zhu, Yiwei , Peng, Ying , Sun, Chaochen , Fu, Fengfu , Lin, Yue . A cost-effective method applicable to the speciation analysis of lead in various types of seafood based on short-column ion chromatography-inductively coupled plasma mass spectrometry . | JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY , 2025 , 40 (5) , 1364-1372 . |
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Real time detection of hydrogen sulfide (H2S) in deep brain regions is of great significance. Photoelectrochemical (PEC) biosensors hold promise for real-time monitoring but low sensitivity due to the use of small electrode restrained its application. In this study, a miniaturized sensor that integrates organic photoelectrochemical transistors (OPECTs) with optical fiber (OF) microelectrodes was designed for the first time, which can amplify the small PEC signal into large channel current change. A conductive gold layer and cuprous oxide nanoparticles (Cu2O NPs) were sequentially deposited onto the OF, followed by a Nafion polymer coating to enhance anti-interference capabilities. Cu2O reacts with H2S to generate Cu2S. The resulting Cu2O/Cu2S heterojunction induces changes in the PEC signal and channel current. Compared to a conventional PEC system, the OPECT sensor has higher sensitivity and lower detection limit, enabling monitoring of H2S concentration fluctuations within the rat brain, which demonstrates the approach's capability for highly sensitive in vivo biomarker detection.
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| GB/T 7714 | Zeng, Yulan , Wang, Xinyang , Chen, Zhonghui et al. Target-Induced Chemical Reaction Modulated Optical Fiber-Gated Organic Photoelectrochemical Transistor for in Vivo H2S Detection [J]. | ANALYTICAL CHEMISTRY , 2025 , 97 (13) : 7467-7475 . |
| MLA | Zeng, Yulan et al. "Target-Induced Chemical Reaction Modulated Optical Fiber-Gated Organic Photoelectrochemical Transistor for in Vivo H2S Detection" . | ANALYTICAL CHEMISTRY 97 . 13 (2025) : 7467-7475 . |
| APA | Zeng, Yulan , Wang, Xinyang , Chen, Zhonghui , Luo, Fang , Qiu, Bin , Fu, Fengfu et al. Target-Induced Chemical Reaction Modulated Optical Fiber-Gated Organic Photoelectrochemical Transistor for in Vivo H2S Detection . | ANALYTICAL CHEMISTRY , 2025 , 97 (13) , 7467-7475 . |
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A simple one-pot method is developed to prepare positively charged aggregated silver nanoparticles (a-AgNPs). The obtained a-AgNPs show strong localized surface plasmon resonance (LSPR) absorption, whose wavelength can be easily tuned to match the commonly used lasers in surface enhanced Raman scattering (SERS). Furthermore, the obtained a-AgNPs can be easily fabricated into paper-based SERS chips by filtering against a negatively charged filter membrane. On the basis, a convenient SERS sensor has been developed for the detection of thiram using a 785 nm handheld Raman spectrometer.
Keyword ：
Aggregated AgNPs Aggregated AgNPs Paper-based chips Paper-based chips Positively charged Positively charged SERS SERS Thiram Thiram
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| GB/T 7714 | Lin, Wei , Liu, Zesong , Zhang, Jingwen et al. Paper-based SERS chips for the rapid detection of thiram [J]. | SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY , 2025 , 332 . |
| MLA | Lin, Wei et al. "Paper-based SERS chips for the rapid detection of thiram" . | SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY 332 (2025) . |
| APA | Lin, Wei , Liu, Zesong , Zhang, Jingwen , Xu, Jinhua , Fu, Fengfu , Lin, Zhenyu et al. Paper-based SERS chips for the rapid detection of thiram . | SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY , 2025 , 332 . |
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The current surface-enhanced Raman scattering (SERS) substrates typically feature a single energy level, posing challenges in coordinating electromagnetic enhancement (EM) and chemical enhancement (CM), thereby limiting the sensitive detection of numerous crucial target molecules. In this study, novel aggregated nanorings (a-NRs) hybridizing Ag, Au and AgCl are constructed as SERS substrates. On one hand, the obtained a-NRs exhibit robust localized surface plasmon resonance absorption, whose wavelength can be tuned to match three commonly used laser wavelengths (532, 633 and 785 nm) to gain strong EM effect. On the other hand, these materials possess the Fermi levels of Au nanoparticles and Au/Ag alloy, in addition to the valence band and conduction band of AgCl. The abundant energy levels of the obtained a-NRs facilitate increased charge transfer opportunities for molecules, leading to a strong CM effect. Therefore, the obtained a-NRs show ultra-high SERS sensitivity towards numerous molecules. Moreover, the unique chemical composition makes the obtained a-NRs have good long-term stability in terms of SERS activity. Besides providing high-performance SERS substrates, the valuable experience for coordinating EM and CM to construct highly active SERS substrate demonstrated in this work are expected to significantly advance the application of SERS.
Keyword ：
Chemical enhancement Chemical enhancement Electromagnetic enhancement Electromagnetic enhancement Energy-level rich Energy-level rich Gold Gold SERS SERS Silver Silver Silver chloride Silver chloride
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| GB/T 7714 | Hu, Rongjing , Fu, Shilan , Zhou, Yongcong et al. Energy-level rich nanorings hybridizing Ag, Au and AgCl as high-performance SERS substrate for numerous molecules [J]. | TALANTA , 2025 , 286 . |
| MLA | Hu, Rongjing et al. "Energy-level rich nanorings hybridizing Ag, Au and AgCl as high-performance SERS substrate for numerous molecules" . | TALANTA 286 (2025) . |
| APA | Hu, Rongjing , Fu, Shilan , Zhou, Yongcong , Lin, Zhenyu , Fu, Fengfu , Dong, Yongqiang . Energy-level rich nanorings hybridizing Ag, Au and AgCl as high-performance SERS substrate for numerous molecules . | TALANTA , 2025 , 286 . |
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A simple method is developed for the direct synthesis of aggregated silver nanoparticles (a-AgNPs), which show strong and wavelength-tunable localized surface plasmon resonance (LSPR). Three a-AgNPs (532-a-AgNPs, 633-a-AgNPs and 787-a-AgNPs) exhibiting the maximum LSPR wavelength coinciding well with those of lasers commonly used in SERS detection (532, 633 and 785 nm) are obtained. Thus, the three a-AgNPs possess abundant plasmonic hotspots under the irradiation of corresponding laser. Moreover, the three a-AgNPs show robust stability in water, and can be developed into liquid substrates directly for SERS determination. All the three substrates (532-sub, 633-sub, and 787-sub) show excellent SERS activity to 4-mercaptobenzoic acid (4-MBA) due to the synergistic effect of strong electromagnetic enhancement (EM) and chemical enhancement (CM) effects. The obtained 787-sub is further applied for the determination of thiram in juices. The low detection limit of 10 ppb and outstanding recoveries of 91.75–109.92 % suggest substantially the reliability and practicality of the developed SERS substrates in food safety detection. © 2025 Elsevier B.V.
Keyword ：
Laser safety Laser safety Metal nanoparticles Metal nanoparticles Silver nanoparticles Silver nanoparticles Surface plasmon resonance Surface plasmon resonance
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| GB/T 7714 | Hu, Rongjing , Zeng, Chenyi , Zhang, Jingwen et al. Stable aggregated silver nanoparticle in water with strong and wavelength-tunable localized surface plasmon resonance for liquid-state SERS substrates [J]. | Journal of Molecular Liquids , 2025 , 424 . |
| MLA | Hu, Rongjing et al. "Stable aggregated silver nanoparticle in water with strong and wavelength-tunable localized surface plasmon resonance for liquid-state SERS substrates" . | Journal of Molecular Liquids 424 (2025) . |
| APA | Hu, Rongjing , Zeng, Chenyi , Zhang, Jingwen , Xu, Jinhua , Pan, Cheng , Lin, Zhenyu et al. Stable aggregated silver nanoparticle in water with strong and wavelength-tunable localized surface plasmon resonance for liquid-state SERS substrates . | Journal of Molecular Liquids , 2025 , 424 . |
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Seafood consumption is the major source of total Hg (tHg) and methyl mercury (MeHg) for humans. Lack of broad-representative bio-accessibility of mercury species makes accurate assessment on health risk of seafood's mercury impossible. Herein, the concentrations and in vitro bio-accessibilities of mercury species in 93 seafood samples with 71 different species were extensively investigated. Results indicated that all shellfish and fish samples, and most seaweed samples contained both Hg2+ and MeHg, while some seaweed samples contained only Hg2+. The concentrations of mercury species varied depending on the differences in species/individuals of seafood and sampling regions. MeHg in seafood can be partly de-methylated into Hg2+ during gastrointestinal digestion, which reduced the toxicity of mercury in seafood. The mean demethylation rate of MeHg varied as follows: seaweeds (similar to 62.1 %) > shellfishes/shrimps (similar to 19.7 %) > fishes (similar to 9.2 %). The mean bio-accessibility of Hg2+ and tHg varied as follows: seaweeds (similar to 97.7 % and similar to 90.1 %) > shellfishes/shrimps (similar to 65.1 % and similar to 67.9 %) approximate to fishes (similar to 65.1 % and similar to 66.7 %), while that of MeHg varied as follows: fishes (similar to 57.7 %) > shellfishes/shrimps (50.8 %) > seaweeds (similar to 11.6 %). The simulated calculation of target hazard quotient (THQ) revealed that the health risk of seafood's mercury may be accurately assessed using tHg, not mercury species, even without considering bio-accessibility. This offers a simple but protective approach for assessing the health risk of seafood's mercury. Results of this study provide the potential broad-representative bio-accessibilities of mercury species existing in various kinds of seafood and novel insights for scientifically assessing the health risk of seafood's mercury and revising the mercury limitation in seafood.
Keyword ：
Bioavailability Bioavailability Marine organism Marine organism Mercury Mercury Risk assessment Risk assessment Seafood Seafood
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| GB/T 7714 | Sun, Chaochen , Yin, Miaomiao , Peng, Ying et al. The characteristic and bio-accessibility evaluation of mercury species in various kinds of seafood collected from Fujian of China for mercury risk assessment [J]. | JOURNAL OF HAZARDOUS MATERIALS , 2025 , 482 . |
| MLA | Sun, Chaochen et al. "The characteristic and bio-accessibility evaluation of mercury species in various kinds of seafood collected from Fujian of China for mercury risk assessment" . | JOURNAL OF HAZARDOUS MATERIALS 482 (2025) . |
| APA | Sun, Chaochen , Yin, Miaomiao , Peng, Ying , Lin, Chen , Wu, Yongning , Fu, Fengfu et al. The characteristic and bio-accessibility evaluation of mercury species in various kinds of seafood collected from Fujian of China for mercury risk assessment . | JOURNAL OF HAZARDOUS MATERIALS , 2025 , 482 . |
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The contamination of micro-plastics (MPs)/nano-plastics (NPs) in environment poses a global concern, necessitating a scientific evaluation of their potential risks to ecosystems and organisms. We herein investigated the adsorption kinetics of different mercury species including inorganic mercury (Hg2+), methylmercury (MeHg) and ethylmercury (EtHg) on three different MPs, such as polyethylene MPs (PE MPs), polypropylene MPs (PP MPs) and polystyrene MPs (PS MPs) in micro-polluted water in detail, and further evaluated the individual and combined cytotoxicity of polystyrene NPs (PS NPs)/polypropylene NPs (PP NPs) and different mercury species. The results indicated that EtHg undergoes demethylation partly to form Hg2+ during the adsorption process on PP MPs, and the adsorption kinetics of Hg2+, MeHg and EtHg on three MPs fitted with pseudo-first-order (PFO) model in initial stage and simultaneously fitted with pseudo-second-order (PSO) model during whole adsorption process. This suggested that adsorption of mercury species on three MPs we driven mainly by physical process in initial stage and by chemical process during whole absorption process. In natural micro-polluted water, the equilibrium adsorption capacities (Qe) of Hg2+ on three MPs (156.3–270.3 ng/g) are much higher than that of MeHg (5.562–78.13 ng/g) and EtHg (7.831–70.42 ng/g). Cytotoxicity experiments revealed that PP NPs and PS NPs themselves have little cytotoxicity, but the presence of them can enhance the cytotoxicity of mercury species, showing NPs size-depended and mercury species-depended synergistic toxic effect. The findings of this study provided valuable insights for scientifically evaluating the potential risk of MPs/NPs to ecosystems and organisms. © 2025 Elsevier B.V.
Keyword ：
Marine pollution Marine pollution Microplastic Microplastic Risk assessment Risk assessment River pollution River pollution Water analysis Water analysis
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| GB/T 7714 | Wu, Siqi , Yang, Chen , Xu, Haochen et al. Adsorption kinetics of different mercury species on three kinds of micro-/nano-plastics in micro-polluted aquatic environments and their combined toxicity [J]. | Science of the Total Environment , 2025 , 981 . |
| MLA | Wu, Siqi et al. "Adsorption kinetics of different mercury species on three kinds of micro-/nano-plastics in micro-polluted aquatic environments and their combined toxicity" . | Science of the Total Environment 981 (2025) . |
| APA | Wu, Siqi , Yang, Chen , Xu, Haochen , Zhu, Yiwei , Fu, FengFu , Lin, Yue . Adsorption kinetics of different mercury species on three kinds of micro-/nano-plastics in micro-polluted aquatic environments and their combined toxicity . | Science of the Total Environment , 2025 , 981 . |
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In recent years, the optical functional materials of iridium(III) complexes have attracted much attention due to their excellent photophysical properties and potential biomedical applications. Among them, aggregation-induced emission (AIE) properties have shown significant advantages in photodynamic therapy (PDT). In this work, three new iridium(III) complexes with near-infrared emission were designed and synthesized, and their crystal structures were determined. All complexes exhibited significant AIE phenomena. Especially, Ir-1 containing an -NH2 functional group exhibited a unique hollow nanoparticle morphology, excellent mitochondrial targeting ability, and high reactive oxygen species (ROS) efficiency, which is expected to play an important role as a new type of nanoprobe in the field of PDT. The complex has the ability to generate both type I and type II ROS, which makes it a potent photosensitizer for the efficient phototherapy of hypoxic tumors. What is more, the complex has dual functions of PDT and chemotherapy, and their synergistic effect greatly enhances the antineoplastic effects of the reagent.
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| GB/T 7714 | Chen, Weibin , Zheng, Qianghui , Zhang, Wanqing et al. Mitochondria-Localized and Aggregation-Induced Near-Infrared Emission Iridium(III) Complexes for Photodynamic Therapy [J]. | INORGANIC CHEMISTRY , 2025 , 64 (28) : 14487-14497 . |
| MLA | Chen, Weibin et al. "Mitochondria-Localized and Aggregation-Induced Near-Infrared Emission Iridium(III) Complexes for Photodynamic Therapy" . | INORGANIC CHEMISTRY 64 . 28 (2025) : 14487-14497 . |
| APA | Chen, Weibin , Zheng, Qianghui , Zhang, Wanqing , Fu, Fengfu , Li, Mei-Jin . Mitochondria-Localized and Aggregation-Induced Near-Infrared Emission Iridium(III) Complexes for Photodynamic Therapy . | INORGANIC CHEMISTRY , 2025 , 64 (28) , 14487-14497 . |
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