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< Page ,Total 31 >
Screening of Cross-Reactive Aptamers for the Detection of 24 Quinolones by Using a Liebig's Law-Guided Parallel-Series Strategy SCIE
期刊论文 | 2024 , 96 (21) , 8576-8585 | ANALYTICAL CHEMISTRY
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Quinolones, a widely used class of antibiotics, present significant environmental and health concerns if they excessively remain in the environment and in food. Aptamers specific to quinolones can be applied as bioreceptors for the detection of quinolone residues in the environment and food. The quinolone family contains dozens of different individuals that share the same core structure coupled with various substituents at six different positions. The diversity and complexity of the substitution sites make it a challenge to choose a set of representative molecules that encompass all the desired sites and preserve the core molecular framework for the screening of quinolone-specific aptamers via systematic evolution of ligands by exponential enrichment (SELEX). To address this challenge, we introduce a novel parallel-series strategy guided by Liebig's law for isolating quinolone-specific cross-reactive aptamers by using the library-immobilized SELEX method. Through this approach, we successfully identified 5 aptamers (Apt.AQ01-Apt.AQ05) with high binding affinity and excellent specificity to 24 different quinolone individuals. Among them, Apt.AQ03 showcased optimal performance with affinities ranging from 0.14 to 1.07 mu M across the comprehensive set of 24 quinolones, exhibiting excellent specificity against nontarget interferents. The binding performance of Apt.AQ03 was further characterized with microscale thermophoresis, circular dichroism spectra, and an exonuclease digestion assay. By using Apt.AQ03 as a bioreceptor, a fluorescence resonance energy transfer (FRET) aptasensor was developed for the detection of 24 quinolones in milk, achieving a remarkable detection limit of 14.5-21.8 ng/mL. This work not only establishes a robust and effective strategy for selecting cross-reactive aptamers applicable to other small-molecule families but also provides high-quality aptamers for developing various high-throughput and reliable methods for the detection of multiple quinolone residues in food.

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GB/T 7714 Yang, Weijuan , Luo, Dongdong , Zheng, Siyu et al. Screening of Cross-Reactive Aptamers for the Detection of 24 Quinolones by Using a Liebig's Law-Guided Parallel-Series Strategy [J]. | ANALYTICAL CHEMISTRY , 2024 , 96 (21) : 8576-8585 .
MLA Yang, Weijuan et al. "Screening of Cross-Reactive Aptamers for the Detection of 24 Quinolones by Using a Liebig's Law-Guided Parallel-Series Strategy" . | ANALYTICAL CHEMISTRY 96 . 21 (2024) : 8576-8585 .
APA Yang, Weijuan , Luo, Dongdong , Zheng, Siyu , Zhang, Yiru , Wang, Zongwen , Fu, Fengfu . Screening of Cross-Reactive Aptamers for the Detection of 24 Quinolones by Using a Liebig's Law-Guided Parallel-Series Strategy . | ANALYTICAL CHEMISTRY , 2024 , 96 (21) , 8576-8585 .
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Mitochondria-targeted ruthenium(ii) complexes for photodynamic therapy and GSH detection in living cells SCIE
期刊论文 | 2024 , 53 (13) , 5957-5965 | DALTON TRANSACTIONS
WoS CC Cited Count: 1
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Photodynamic therapy is an emerging tumor therapy that kills tumor cells by activating reactive oxygen species (ROS) produced by photosensitizers. Mitochondria, as an important organelle, are the main generator of cellular ROS. Therefore, the development of photosensitizers capable of targeting mitochondria could significantly enhance the efficacy of photodynamic therapy. In this study, two novel ruthenium(ii) complexes, Ru-1 and Ru-2, were designed and synthesized, both of which were functionalized with alpha,beta-unsaturated ketones for sensing of glutathione (GSH). The crystal structures of the two complexes were determined and they exhibited good recognition of GSH by off-on luminescence signals. The complex Ru-2 containing aromatic naphthalene can enter the cells and react with GSH to generate a strong luminescence signal that can be used to monitor intracellular GSH levels through imaging. Ru-2 also has an excellent mitochondrial localization ability with a Pearson's coefficient of 0.95, which demonstrates that it can efficiently target the mitochondria of tumor cells to enhance the effectiveness of photodynamic therapy as a photosensitizer.

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GB/T 7714 Zhang, Wanqing , Chen, Weibin , Fu, Fengfu et al. Mitochondria-targeted ruthenium(ii) complexes for photodynamic therapy and GSH detection in living cells [J]. | DALTON TRANSACTIONS , 2024 , 53 (13) : 5957-5965 .
MLA Zhang, Wanqing et al. "Mitochondria-targeted ruthenium(ii) complexes for photodynamic therapy and GSH detection in living cells" . | DALTON TRANSACTIONS 53 . 13 (2024) : 5957-5965 .
APA Zhang, Wanqing , Chen, Weibin , Fu, Fengfu , Li, Mei-Jin . Mitochondria-targeted ruthenium(ii) complexes for photodynamic therapy and GSH detection in living cells . | DALTON TRANSACTIONS , 2024 , 53 (13) , 5957-5965 .
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MoS2 Nanosheets as Substrates for SERS-Based Sensing SCIE
期刊论文 | 2024 , 7 (4) , 3988-3996 | ACS APPLIED NANO MATERIALS
WoS CC Cited Count: 1
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The chemical enhancement of semiconductor-based surface-enhanced Raman scattering (SERS) substrates is an exciting hot topic. Herein, a simple hydrothermal method is developed to prepare molybdenum disulfide (MoS2), which can be easily exfoliated into monolayer nanosheets (MoS(2)NSs) by sonication, even in the absence of any surfactant. The obtained MoS(2)NSs contain two types of defects, namely, one caused by the incorporation of Mo atoms of high valence states and one caused by the incorporation of S-2(2-). The density of the two types of defects can be easily tuned by controlling the ratio of Na2S and Na2MoO4 in the raw materials. The unique properties and the clear surface make the obtained MoS(2)NSs ideal models to investigate the effect of defects on the SERS activity of MoS2. It is found that the SERS activity of the obtained MoS(2)NSs increases dramatically with the defects caused by Mo atoms of high valence states, while it first increases and then decreases with the increase of defects caused by S-2(2-). On the basis, MoS(2)NSs with high SERS activity and a low detection limit of 5.0 x 10(-9) mol/L toward crystal violet (CV) are obtained. Moreover, the mechanism of defects affecting the SERS activity of MoS(2)NSs is revealed. The defects on one hand provide a large amount of dangling bonds that can combine CV molecules to form MoS2NS-CV complex and on the other hand provide extensive induced local dipoles and enhance the overall SERS spectrum of CV.

Keyword :

chemical enhancement chemical enhancement crystal violet crystal violet defects defects molybdenum disulfidenanosheets molybdenum disulfidenanosheets surface-enhanced Raman scattering surface-enhanced Raman scattering

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GB/T 7714 Fu, Xiaolong , Wu, Huiying , Liu, Zhihong et al. MoS2 Nanosheets as Substrates for SERS-Based Sensing [J]. | ACS APPLIED NANO MATERIALS , 2024 , 7 (4) : 3988-3996 .
MLA Fu, Xiaolong et al. "MoS2 Nanosheets as Substrates for SERS-Based Sensing" . | ACS APPLIED NANO MATERIALS 7 . 4 (2024) : 3988-3996 .
APA Fu, Xiaolong , Wu, Huiying , Liu, Zhihong , Wang, Pengzhao , Rong, Jiefeng , Fu, Fengfu et al. MoS2 Nanosheets as Substrates for SERS-Based Sensing . | ACS APPLIED NANO MATERIALS , 2024 , 7 (4) , 3988-3996 .
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Effect of inorganic arsenic in paddy soil on the migration and transformation of selenium species in rice plants SCIE CSCD
期刊论文 | 2024 , 143 , 35-46 | JOURNAL OF ENVIRONMENTAL SCIENCES
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Selenium (Se) in paddy rice is one of the significant sources of human Se nutrition. However, the effect of arsenic (As) pollution in soil on the translocation of Se species in rice plants is unclear. In this research, a pot experiment was designed to examine the effect of the addition of 50 mg As/kg soil as arsenite or arsenate on the migration of Se species from soil to indica Minghui 63 and Luyoumingzhan. The results showed that the antagonism between inorganic As and Se was closely related to the rice cultivar and Se oxidation state in soil. Relative to the standalone selenate treatment, arsenite significantly ( p < 0.05) decreased the accumulation of selenocystine, selenomethionine and selenate in the roots, stems, sheaths, leaves, brans and kemels of both cultivars by 21.4%-100.0%, 40.0%-100.0%, 41.0%-100%, 5.4%96.3%, 11.3%-100.0% and 26.2%-39.7% respectively, except for selenocystine in the kemels of indica Minghui 63 and selenomethionine in the leaves of indica Minghui 63 and the stems of indica Luyoumingzhan. Arsenate also decreased ( p < 0.05) the accumulation of selenocystine, selenomethionine and selenate in the roots, stems, brans and kernels of both cultivars by 34.9%-100.0%, 30.2%-100.0%, 11.3%-100.0% and 5.6%-39.6% respectively, except for selenate in the stems of indica Minghui 63. However, relative to the standalone selenite treatment, arsenite and arsenate decreased ( p < 0.05) the accumulation of selenocystine, selenomethionine and selenite only in the roots of indica Minghui 63 by 45.5%-100.0%. Our results suggested that arsenite and arsenate had better antagonism toward Se species in selenate-added soil than that in selenite-added soil; moreover, arsenite had a higher inhibiting effect on the accumulation of Se species than arsenate. (c) 2024 The Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences. Published by Elsevier B.V.

Keyword :

Antagonistic effect Antagonistic effect Inorganic arsenic Inorganic arsenic Rice( Oryza sativa L.) Rice( Oryza sativa L.) Selenium species Selenium species Translocation Translocation

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GB/T 7714 Pokhrel, Ganga Raj , Wang, Kaiteng , Ying, Kaiyang et al. Effect of inorganic arsenic in paddy soil on the migration and transformation of selenium species in rice plants [J]. | JOURNAL OF ENVIRONMENTAL SCIENCES , 2024 , 143 : 35-46 .
MLA Pokhrel, Ganga Raj et al. "Effect of inorganic arsenic in paddy soil on the migration and transformation of selenium species in rice plants" . | JOURNAL OF ENVIRONMENTAL SCIENCES 143 (2024) : 35-46 .
APA Pokhrel, Ganga Raj , Wang, Kaiteng , Ying, Kaiyang , Wu, Yongchen , Wang, Ze , Zhu, Xi et al. Effect of inorganic arsenic in paddy soil on the migration and transformation of selenium species in rice plants . | JOURNAL OF ENVIRONMENTAL SCIENCES , 2024 , 143 , 35-46 .
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Pargyline-phosphine copper(I) clusters with tunable emission for light-emitting devices SCIE
期刊论文 | 2024 , 53 (13) , 5844-5850 | DALTON TRANSACTIONS
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Three pargyline-phosphine copper(I) clusters, [Cu-4(C equivalent to C-C9H12N)(3)(PPh3)(4)](PF6) (1) and [Cu-6(CC-C9H12N)(4)(dppy)(4)](X)(2) (dppy = diphenyl-2-pyridylphosphine; X = PF6 for 2 and X = ClO4 for 3), were synthesized. Their structures were fully characterized using various spectroscopic methods and X-ray crystallography, which showed that the stoichiometry and nature of pargyline and phosphine ligands play an important role in tuning the structure and photophysical features of Cu(i) clusters. Interestingly, clusters 1, 2 and 3 exhibited red, orange and yellow phosphorescence with high quantum yields of 88.5%, 22.0% and 40.2%, respectively, at room temperature. Moreover, clusters 1-3 show distinct temperature-dependent emissions. The excellent luminescence performance of 1 and 3 was designed and employed for the construction of monochrome and white light-emitting devices (LEDs).

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GB/T 7714 Huang, Qiu-Qin , Lin, Yan-Yan , Wang, Yu-Ling et al. Pargyline-phosphine copper(I) clusters with tunable emission for light-emitting devices [J]. | DALTON TRANSACTIONS , 2024 , 53 (13) : 5844-5850 .
MLA Huang, Qiu-Qin et al. "Pargyline-phosphine copper(I) clusters with tunable emission for light-emitting devices" . | DALTON TRANSACTIONS 53 . 13 (2024) : 5844-5850 .
APA Huang, Qiu-Qin , Lin, Yan-Yan , Wang, Yu-Ling , Qi, Jia yuan , Fu, FengFu , Wei, Qiao-Hua . Pargyline-phosphine copper(I) clusters with tunable emission for light-emitting devices . | DALTON TRANSACTIONS , 2024 , 53 (13) , 5844-5850 .
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Gold nanoparticle-loaded MoS2 nanosheets with peroxidase-like and pyranose oxidase-like activities for bio-enzyme-free visual detection of glucose, xylose and galactose ESCI
期刊论文 | 2024 , 5 (10) , 4221-4230 | MATERIALS ADVANCES
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Enzyme mimics with dual enzyme-like activities can catalyse cascade reactions with high efficiency and thus play a significant role in biochemistry since multistep cascade reactions often occur in biocatalysis. Especially, a nanozyme that simultaneously possesses peroxidase-like and pyranose oxidase (POx)-like activities is highly desired since it can be used for bio-synthetizing rare sugars and fabricating bio-enzyme-free colorimetric methods for the detection of various pyranoses. We herein prepared a novel dual-active nanozyme, which simultaneously possesses enhanced and stable peroxidase-like and POx-like activities, by loading gold nanoparticles (AuNPs) on MoS2 nanosheets (AuNPs@MoS2). The prepared AuNPs@MoS2 nanozyme can catalyse various tandem reactions of pyranose oxidation and H2O2-mediated oxidation of TMB with high efficiency and, therefore, can be used to fabricate bio-enzyme-free colorimetric methods for the detection of various monosaccharides with a pyranyl ring, including glucose, xylose and galactose. Based on the AuNPs@MoS2 nanozyme, we successfully developed bio-enzyme-free colorimetric methods for the detection of glucose, xylose and galactose with a visual detection limit of 0.2-0.3 mM and a spectrometry detection limit of 5.0-11 mu M. The developed colorimetric glucose, xylose and galactose detection methods were successfully used to detect glucose in serum, xylose in bread and galactose in milk, respectively, with a recovery of 89-108% and a relative standard deviation (RSD, n = 5) of <5%. With enhanced peroxidase-like and POx-like activities and good stability, the developed AuNPs@MoS2 provided a promising dual-active nanozyme for the bio-enzyme-free catalysis of various cascade reactions for the oxidation of various monosaccharides with a pyranyl ring and for further fabricating bio-enzyme-free, cost-effective and simple colorimetric sensors for the visual detection of various monosaccharides with a pyranyl ring, including glucose, xylose and galactose.

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GB/T 7714 Fu, Shilan , Liu, Junfeng , Wu, Siqi et al. Gold nanoparticle-loaded MoS2 nanosheets with peroxidase-like and pyranose oxidase-like activities for bio-enzyme-free visual detection of glucose, xylose and galactose [J]. | MATERIALS ADVANCES , 2024 , 5 (10) : 4221-4230 .
MLA Fu, Shilan et al. "Gold nanoparticle-loaded MoS2 nanosheets with peroxidase-like and pyranose oxidase-like activities for bio-enzyme-free visual detection of glucose, xylose and galactose" . | MATERIALS ADVANCES 5 . 10 (2024) : 4221-4230 .
APA Fu, Shilan , Liu, Junfeng , Wu, Siqi , Zhang, Lin , Zhang, Xu , Fu, Fengfu . Gold nanoparticle-loaded MoS2 nanosheets with peroxidase-like and pyranose oxidase-like activities for bio-enzyme-free visual detection of glucose, xylose and galactose . | MATERIALS ADVANCES , 2024 , 5 (10) , 4221-4230 .
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Bio-accessibility and bio-availability evaluation of each arsenic species existing in various edible seaweeds in vitro and in vivo for arsenic risk assessment Scopus
期刊论文 | 2024 , 946 | Science of the Total Environment
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Seaweeds consumption is one of main internal exposure sources of arsenic for human. However, the absence of representative bio-availabilities of arsenic species makes the accurate assessment of arsenic health risk originating from seaweeds consumption impossible. Herein, the arsenic species in various seaweeds collected from Fujian of China were investigated, and the bio-accessibilities/bio-availabilities of arsenic species existing in seaweeds were evaluated in vitro and in vivo. Results revealed that in vitro bio-availabilities of arsenic species presenting in seaweeds, which obtained with Caco-2 cells, were lower than those of pure arsenic standards, and varied with order of inorganic arsenic (iAs) > dimethylarsinic acid (DMA) ≈ arsenobetaine (AsB) > arsenosugars. During gastrointestinal digestion of mice, As5+ was partly methylated into monomethylarsonic acid (MMA) and DMA, which makes the in vivo bioavailability of iAs (⁓31.8 %) obtained with mouse metabolic experiment is much higher than its in vitro bio-availability (⁓10.3 %). The in vivo bio-availabilities of DMA and total arsenic (tAs) are similar to their in vitro bio-availabilities. As the dominant arsenic species in most seaweeds, arsenosugars have an ⁓0.0 % of in vivo bioavailability and only a ⁓3.7 % of in vitro bioavailability. The simulated calculation of target hazard quotient (THQ) and target cancer risk (TR) revealed that the arsenic risk originating from seaweeds was greatly degraded by taking into consideration of arsenic species and bio-availabilities, and all seaweeds collected from Fujian are safety for consumption. The simulated calculation also revealed that arsenic risk of seaweeds can be also more accurately assessed based on tAs together with bioavailability, which provides a simple but accurate and protective method for the risk assessment of arsenic originating from seaweeds. Our work provides the possible representative bio-availabilities of arsenic species presenting in seaweeds for accurately assessing arsenic risk of seaweeds, and novel insights into the bio-availabilities of arsenic in animal. © 2024 Elsevier B.V.

Keyword :

Arsenic Arsenic Bio-accessibility Bio-accessibility Bio-availability Bio-availability Risk assessment Risk assessment Seafood Seafood

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GB/T 7714 Lin, Y. , Liu, J. , Sun, Y. et al. Bio-accessibility and bio-availability evaluation of each arsenic species existing in various edible seaweeds in vitro and in vivo for arsenic risk assessment [J]. | Science of the Total Environment , 2024 , 946 .
MLA Lin, Y. et al. "Bio-accessibility and bio-availability evaluation of each arsenic species existing in various edible seaweeds in vitro and in vivo for arsenic risk assessment" . | Science of the Total Environment 946 (2024) .
APA Lin, Y. , Liu, J. , Sun, Y. , Chen, S. , Chen, J. , Fu, F. . Bio-accessibility and bio-availability evaluation of each arsenic species existing in various edible seaweeds in vitro and in vivo for arsenic risk assessment . | Science of the Total Environment , 2024 , 946 .
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Isolation of aptamers with excellent cross-reactivity and specificity to sulfonamides towards a ratiometric fluorescent aptasensor for the detection of nine sulfonamides in seafood EI
期刊论文 | 2024 , 277 | Talanta
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Sulfonamides (SAs) is a class of antibiotics that extensively used for treating infectious diseases in livestock industries and aquaculture. Thus, it is urgent need to obtain the bio-receptor, which has excellent cross-reactivity and specificity to SAs, for developing high-throughput methods for the determination of multiple SAs even all commonly-used SAs, to realize the quick screening/detection of total SAs in animal-derived foods. We herein isolated several SAs-specific cross-reactive aptamers by using a library-immobilized SELEX with multi-SAs parallel selection strategy. Two of the isolated aptamers (Sul-01 and Sul-04) can specifically recognize and bind seven SAs respectively with higher binding affinity and no interference of non-sulfonamide antibiotics, and thus can be applied as bio-receptors for developing high-throughput aptasensors for the quick screening/detection of multiple SAs. By using the mixture of Sul-01 and Sul-04 as bio-receptor, a ratiometric fluorescent aptasensor was created for the quick detection of nine SAs including sulfamethoxydiazine (SMD), sulfapyridine (SPD), sulfaquinoxaline (SQ), sulfathiazole (ST), sulfamonomethoxine (SMM), sulfamerazine (SMR), sulfaguanidine (SG), sulfamethazine (SMZ) and sulfadiazine (SD) with a detection limit (LOD) of 0.10–0.50 μM, or total of above nine SAs with a LOD of 0.20 μM. The fluorescent aptasensor was successfully applied to detect each or total of SMD, SPD, SQ, ST, SMM, SMR, SG, SMZ and SD in fish samples with a recovery of 83 %–92 % and a relative standard deviation (RSD, n = 5) © 2024 Elsevier B.V.

Keyword :

Agriculture Agriculture Amides Amides Antibiotics Antibiotics Aquaculture Aquaculture Binding energy Binding energy Diagnosis Diagnosis Diseases Diseases Fish Fish Fluorescence Fluorescence Meats Meats Sulfur compounds Sulfur compounds

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GB/T 7714 Zhang, Lin , Wu, Siqi , Liu, Junfeng et al. Isolation of aptamers with excellent cross-reactivity and specificity to sulfonamides towards a ratiometric fluorescent aptasensor for the detection of nine sulfonamides in seafood [J]. | Talanta , 2024 , 277 .
MLA Zhang, Lin et al. "Isolation of aptamers with excellent cross-reactivity and specificity to sulfonamides towards a ratiometric fluorescent aptasensor for the detection of nine sulfonamides in seafood" . | Talanta 277 (2024) .
APA Zhang, Lin , Wu, Siqi , Liu, Junfeng , Ping, Meiling , Yang, Weijuan , Fu, FengFu . Isolation of aptamers with excellent cross-reactivity and specificity to sulfonamides towards a ratiometric fluorescent aptasensor for the detection of nine sulfonamides in seafood . | Talanta , 2024 , 277 .
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Rapid detection of tebuconazole based on hydrogel SERS chips SCIE
期刊论文 | 2024 , 277 | TALANTA
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Tebuconazole is one of the most commonly used fungicides in agricultural production, that has the merits of highly effectiveness, broad spectrum and systemic function. Excessive tebuconazole may pose a great threat to human and animal health. Traditional detection techniques for tebuconazole usually have limitations such as expensive equipment, poor antibody stability, and time-consuming procedures. Herein, a sensitive sensor is developed for the rapid detection of tebuconazole based on hydrogel surface-enhanced Raman scattering (SERS) chips. Aggregated Ag nanoparticles (a-AgNPs) with tunable localized surface plasmon resonance (LSPR) wavelength are in-situ synthesized in polyvinyl alcohol (PVA) solution for preparing hydrogel SERS chips. Three hydrogel SERS chips are obtained to match the three commonly used laser wavelengths. On the basis, a match laser wavelength is selected according to the energy levels of tebuconazole and the Fermi level of a-AgNPs to gain a strong chemical enhancement. At the same time, the chip with a corresponding LSPR wavelength to the laser is applied to obtain a strong electromagnetic enhancement. Thus, highly sensitive SERS signal of tebuconazole is obtained. Furthermore, the obtained hydrogel SERS chips have good repeatability, outstanding reproducibility and strong anti-interference ability, and show outstanding reliability in practical applications. As a result, the SERS chips offer a reliable and convenient platform for the quick detection of tebuconazole in foods. The detection limit is as low as 1 ppb, and the recoveries is distributed in the range of 94.66-106.70 %. This work would promote greatly the application of SERS in small molecule detection.

Keyword :

Aggregated AgNPs Aggregated AgNPs PVA hydrogel PVA hydrogel Surface-enhanced Raman scattering Surface-enhanced Raman scattering Tebuconazole Tebuconazole Tunable localized surface plasmon resonance Tunable localized surface plasmon resonance

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GB/T 7714 Chen, Mingming , Lai, Xiaojing , Su, Bihang et al. Rapid detection of tebuconazole based on hydrogel SERS chips [J]. | TALANTA , 2024 , 277 .
MLA Chen, Mingming et al. "Rapid detection of tebuconazole based on hydrogel SERS chips" . | TALANTA 277 (2024) .
APA Chen, Mingming , Lai, Xiaojing , Su, Bihang , Jiang, Xiancai , Xu, Jinhua , Fu, Fengfu et al. Rapid detection of tebuconazole based on hydrogel SERS chips . | TALANTA , 2024 , 277 .
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Dual-Loading of Fe3O4 and Pd Nanoparticles on g-C3N4 Nanosheets Toward a Magnetic Nanoplatform with Enhanced Peroxidase-like Activity for Loading Various Enzymes for Visual Detection of Small Molecules SCIE
期刊论文 | 2023 , 95 (11) , 5024-5033 | ANALYTICAL CHEMISTRY
WoS CC Cited Count: 18
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Enzyme mimics now play a significant role in biochemistry. Especially, peroxidase mimics have been widely used for developing colorimetric sensors of blood glucose. The peroxidase mimics previously reported could not be recycled for reusing and may generate scattering to cause unwanted optical interference when it was used for fabricating colorimetric sensors. We herein prepared a broad-applicable and reusable magnetic enzyme-loading nanoplatform with enhanced peroxidase-like activity by simultaneously loading Fe3O4 nanoparticles (Fe3O4NPs) and palladium nanoparticles (PdNPs) on graphitic carbon nitride (g-C3N4) nanosheets (Fe3O4NPs/PdNPs/g-C3N4). The prepared Fe3O4NPs/PdNPs/g-C3N4 possesses stable and enhanced peroxidase-like activity and good enzyme-loading capacity and can be used to load various natural enzymes to form highly-efficient and stable double-active nanozyme for fabricating colorimetric sensors for the visual detection of small molecules. Especially, the magnetic feature facilitates the magnetic separation of Fe3O4NPs/PdNPs/ g-C3N4 from sample solution, which is in favor of recycling and eliminating the optical interference caused by nanozyme in colorimetric sensors. The prepared Fe3O4NPs/PdNPs/g-C3N4 has been successfully used to load glucose oxidase (GOx) and cholesterol oxidase (Chox) to form magnetic peroxidase-GOx and peroxidase-Chox double-active nanozymes, which can be used to fabricate colorimetric methods for the detection of glucose and cholesterol, respectively, with a visual detection limit of 15 mu M and a spectrometry detection limit of 1.0 mu M. With the developed glucose and cholesterol detection methods, we have successfully detected glucose and cholesterol in serum with a recovery of 98-104% and a RSD (n = 5) < 5%. With high peroxidase-like activity, good stability, reusable features, and broad applicability of loading enzyme, the developed magnetic Fe3O4NPs/PdNPs/g-C3N4 provided a promising approach for fabricating cost-effective, sensitive, and simple colorimetric sensors for the visual detection of various small molecules.

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GB/T 7714 Zhang, Xu , Sun, Chaochen , Li, Ruiling et al. Dual-Loading of Fe3O4 and Pd Nanoparticles on g-C3N4 Nanosheets Toward a Magnetic Nanoplatform with Enhanced Peroxidase-like Activity for Loading Various Enzymes for Visual Detection of Small Molecules [J]. | ANALYTICAL CHEMISTRY , 2023 , 95 (11) : 5024-5033 .
MLA Zhang, Xu et al. "Dual-Loading of Fe3O4 and Pd Nanoparticles on g-C3N4 Nanosheets Toward a Magnetic Nanoplatform with Enhanced Peroxidase-like Activity for Loading Various Enzymes for Visual Detection of Small Molecules" . | ANALYTICAL CHEMISTRY 95 . 11 (2023) : 5024-5033 .
APA Zhang, Xu , Sun, Chaochen , Li, Ruiling , Wu, Yongning , Jin, Xin , Fu, FengFu . Dual-Loading of Fe3O4 and Pd Nanoparticles on g-C3N4 Nanosheets Toward a Magnetic Nanoplatform with Enhanced Peroxidase-like Activity for Loading Various Enzymes for Visual Detection of Small Molecules . | ANALYTICAL CHEMISTRY , 2023 , 95 (11) , 5024-5033 .
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