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学者姓名：陈岚岚
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Abstract ：
It remains a challenge to use a single probe to simultaneously detect extracellular pH fluctuations and specifically recognize cancer cells for precise drug delivery. Here, we engineered a tetrahedral framework nucleic acid-based logic nanoprobe (isgc8-tFNA) on live cell membranes for simultaneously monitoring extracellular pH and targeted drug delivery. Isgc8-tFNA was anchored stably on the cell surface through three cholesterol molecules inserting into the bilayer of the cell membrane. Once responding to the acidic tumor microenvironment, isgc8-tFNA formed an i-motif structure, leading to turn-on FRET signals for monitoring changes of extracellular pH. The nanoprobe exhibited a narrow pH-response window and excellent reversibility. Moreover, the nanoprobe could execute logic identification on the cell surface for precise drug delivery. Only if both in the acidic microenvironment and aptamer-targeting marker are present on the cell surface, the sgc8-ASO-chimera strand, carrying an antisense oligonucleotide drug, was released from the nanoprobe and entered into targeted cancer cells for gene silence. Additionally, the in situ drug release facilitated the uptake of drugs mediated by the interaction between sgc8 aptamer and membrane proteins, resulting in enhanced inhibition of cancer cell migration and proliferation. This logic nanoprobe will provide inspiration for designing smart devices for diagnosis of pH-related diseases and targeted drug delivery.
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| GB/T 7714 | Chen, Wanzhen , Lai, Jingjing , Dong, Siqi et al. Engineering Logic DNA Nanoprobes on Live Cell Membranes for Simultaneously Monitoring Extracellular pH and Precise Drug Delivery [J]. | ANALYTICAL CHEMISTRY , 2024 , 96 (8) : 3462-3469 . |
| MLA | Chen, Wanzhen et al. "Engineering Logic DNA Nanoprobes on Live Cell Membranes for Simultaneously Monitoring Extracellular pH and Precise Drug Delivery" . | ANALYTICAL CHEMISTRY 96 . 8 (2024) : 3462-3469 . |
| APA | Chen, Wanzhen , Lai, Jingjing , Dong, Siqi , Chen, Lanlan , Yang, Huanghao . Engineering Logic DNA Nanoprobes on Live Cell Membranes for Simultaneously Monitoring Extracellular pH and Precise Drug Delivery . | ANALYTICAL CHEMISTRY , 2024 , 96 (8) , 3462-3469 . |
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Helicobacter Pylori infection is drawing increasing attentions in public health, especially the drug resistance problems induced by Single-Nucleotide Variants (SNV). Diagnosis of H. Pylori remains challenging for its requirement in selectivity and sensitivity. Herein an initial check-reexamination strategy is designed for analysis of H. Pylori DNA and SNV. At the first stage, target DNA with all genotypes is captured to form a Y-shaped structure, resulting in an electrochemiluminescence (ECL) signal recovered from quenched states. Then Cas9 assisted cleavage processes are followed to cut off the Y-shaped structure, resulting in corresponding signal decrease. By means of these two stages with different selectivity, both the total amount of H. Pylori DNA and the ratio of SNV can be clarified. To expand its capacity, a large-scale screening assay is carried out on chip. Array detection improves the reliability and the following PCA analysis confirms the otherness. This approach improved the work efficiency and reduced the cost, which may offer an appealing option for the prevention and cure of H. pylori infections in the future.
Keyword ：
CRISPR/Cas9 CRISPR/Cas9 Electrochemiluminescence Electrochemiluminescence Helicobacter pylori Helicobacter pylori Single-nucleotide variants Single-nucleotide variants
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| GB/T 7714 | Hu, Shanwen , Zhong, Xinyi , Deng, Yuan et al. An initial check-reexamination strategy for analysis of H. Pylori DNA and single-nucleotide variants [J]. | SENSORS AND ACTUATORS B-CHEMICAL , 2024 , 398 . |
| MLA | Hu, Shanwen et al. "An initial check-reexamination strategy for analysis of H. Pylori DNA and single-nucleotide variants" . | SENSORS AND ACTUATORS B-CHEMICAL 398 (2024) . |
| APA | Hu, Shanwen , Zhong, Xinyi , Deng, Yuan , Deng, Yuhang , Chen, Lanlan . An initial check-reexamination strategy for analysis of H. Pylori DNA and single-nucleotide variants . | SENSORS AND ACTUATORS B-CHEMICAL , 2024 , 398 . |
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本研究建立了一种利用催化发夹自组装反应阵列对多种微生物进行联合检测的新方法.该方法采用双靶点策略,第一个靶点为原核生物 16S rRNA的一段保守序列,用于初步判定原核生物,第二个靶点分别为大肠埃希氏菌、单核增生李斯特菌、金黄色葡萄球菌的特异性序列,用于确证微生物种类.双靶点识别后,释放引发链触发催化发夹自组装反应,循环打开带有FAM荧光基团和猝灭基团标记的发夹探针,产生荧光信号.优化反应条件,评价信号的稳定性后,建立对靶标序列检测的工作曲线,通过荧光信号实现对多种微生物的鉴定和定量检测,并建立检测阵列.本方法在靶标浓度为3～50 nmol/L范围内具有良好的线性关系,检出限为0.001 nmol/L.在阵列检测中,本方法能够有效区分3种目标微生物,为多种病原微生物的同时检测提供了新的技术思路.
Keyword ：
催化发夹自组装 催化发夹自组装 病原微生物 病原微生物 阵列检测 阵列检测
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| GB/T 7714 | 黄敏芳 , 庄妙慧 , 张习梅 et al. 基于催化发夹自组装的多种病原微生物阵列检测 [J]. | 中国口岸科学技术 , 2024 , 6 (9) : 70-77 . |
| MLA | 黄敏芳 et al. "基于催化发夹自组装的多种病原微生物阵列检测" . | 中国口岸科学技术 6 . 9 (2024) : 70-77 . |
| APA | 黄敏芳 , 庄妙慧 , 张习梅 , 陈岚岚 . 基于催化发夹自组装的多种病原微生物阵列检测 . | 中国口岸科学技术 , 2024 , 6 (9) , 70-77 . |
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Bacterial infections can easily occur when patients mishandle wounds or eat moldy food. The prompt diagnosis of a bacterial infection could effectively reduce the risk of possible anatomical damage. However, non-invasive early detection of bacterial infections is difficult to achieve due to the lack of favorable tools. Here, we designed two hNQO1 fluorescent probes (RX2 and RX3) to visualize bacterial infection after deep learning on the pathogenesis of bacterial infection. RX2 and RX3 enable early detection of bacterial infection and are verified to be, respectively, suitable for fluorescence imaging (FLI) and photoacoustic imaging (PAI) by comparing the signal-to-background ratio of both probes in a mouse model of myositis caused by Escherichia coli infection. In view of the difference in penetration depth between the two imaging modalities, we further applied RX2 for FLI of E. coli-infected wounds and RX3 for PAI of E. coli-infected inflammatory bowel disease, suggesting the great potential of both probes for early diagnosis of bacterial infections.
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| GB/T 7714 | Chen, Lanlan , Yuan, Lin , Zuo, Shan et al. Family of hNQO1 Activatable Near-Infrared Fluoro-Photoacoustic Probes for Diagnosis of Wound Infection and Ulcerative Colitis [J]. | ANALYTICAL CHEMISTRY , 2023 , 95 (2) : 898-906 . |
| MLA | Chen, Lanlan et al. "Family of hNQO1 Activatable Near-Infrared Fluoro-Photoacoustic Probes for Diagnosis of Wound Infection and Ulcerative Colitis" . | ANALYTICAL CHEMISTRY 95 . 2 (2023) : 898-906 . |
| APA | Chen, Lanlan , Yuan, Lin , Zuo, Shan , Jiang, Gangwei , Zheng, Yingxin , Zhang, Xingxing et al. Family of hNQO1 Activatable Near-Infrared Fluoro-Photoacoustic Probes for Diagnosis of Wound Infection and Ulcerative Colitis . | ANALYTICAL CHEMISTRY , 2023 , 95 (2) , 898-906 . |
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Molecular imaging is a non-invasive method to image and analyze the concentration and activity of functional biomolecules in cells or in vivo at molecular level, and plays an increasing role in deep understanding of biological processes, early and accurate diagnosis of diseases, and evaluation of treatment. Nowadays, numerous novel molecular imaging probes have been developed, involving every biomedical imaging modality, such as optical imaging, photoacoustic imaging, magnetic resonance imaging, single-photon-emission computed tomography, and positron emission tomography. In this review, we summarize the development of current state-of-the-art molecular imaging probes. We introduce the design strategies of molecular probes and detailed imaging modalities, and highlight the properties of probes and biomedical imaging applications in cells and in vivo, including disease diagnosis, drug tracking, and imaging-guided surgery. Then we discuss the perspectives and challenges in this emerging field. We expect this review could inspire more effective molecular imaging probes to be developed, achieving the goal towards clinical practices.
Keyword ：
biomedical applications biomedical applications diseases diagnosis diseases diagnosis imaging probes imaging probes molecular imaging molecular imaging multimodality multimodality
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| GB/T 7714 | Chen, Lanlan , Lyu, Yifan , Zhang, Xuan et al. Molecular imaging: design mechanism and bioapplications [J]. | SCIENCE CHINA-CHEMISTRY , 2023 , 66 (5) : 1336-1383 . |
| MLA | Chen, Lanlan et al. "Molecular imaging: design mechanism and bioapplications" . | SCIENCE CHINA-CHEMISTRY 66 . 5 (2023) : 1336-1383 . |
| APA | Chen, Lanlan , Lyu, Yifan , Zhang, Xuan , Zheng, Liting , Li, Qingqing , Ding, Ding et al. Molecular imaging: design mechanism and bioapplications . | SCIENCE CHINA-CHEMISTRY , 2023 , 66 (5) , 1336-1383 . |
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Surface-enhanced Raman scattering (SERS) is a feasible and ultra-sensitive method for biomedical imaging and disease diagnosis. SERS is widely applied to in vivo imaging due to the development of functional nanoparticles encoded by Raman active molecules (SERS nanoprobes) and improvements in instruments. Herein, the recent developments in SERS active materials and their in vivo imaging and biosensing applications are overviewed. Various SERS substrates that have been successfully used for in vivo imaging are described. Then, the applications of SERS imaging in cancer detection and in vivo intraoperative guidance are summarized. The role of highly sensitive SERS biosensors in guiding the detection and prevention of diseases is discussed in detail. Moreover, its role in the identification and resection of microtumors and as a diagnostic and therapeutic platform is also reviewed. Finally, the progress and challenges associated with SERS active materials, equipment, and clinical translation are described. The present evidence suggests that SERS could be applied in clinical practice in the future.
Keyword ：
biosensing biosensing diagnosis diagnosis Raman mapping Raman mapping self-assembly self-assembly surface-enhanced Raman scattering surface-enhanced Raman scattering
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| GB/T 7714 | Li, Qingqing , Huo, Hongqi , Wu, Ying et al. Design and Synthesis of SERS Materials for In Vivo Molecular Imaging and Biosensing [J]. | ADVANCED SCIENCE , 2023 , 10 (8) . |
| MLA | Li, Qingqing et al. "Design and Synthesis of SERS Materials for In Vivo Molecular Imaging and Biosensing" . | ADVANCED SCIENCE 10 . 8 (2023) . |
| APA | Li, Qingqing , Huo, Hongqi , Wu, Ying , Chen, Lanlan , Su, Lichao , Zhang, Xuan et al. Design and Synthesis of SERS Materials for In Vivo Molecular Imaging and Biosensing . | ADVANCED SCIENCE , 2023 , 10 (8) . |
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We developed a circular bivalent aptamer (CBA) to precisely activate membrane receptor-mediated regenerative signaling for liver repair in vivo. The CBA showed enhanced biostability and receptor binding avidity, achieving effective pathway activation and satisfactory treatment in an acetaminophen-induced liver injury model. This work expands aptamer-based molecular engineering in regenerative medicine.
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| GB/T 7714 | Liang, Hong , Yan, Zhike , Tong, Yuhong et al. Circular bivalent aptamers enhance the activation of the regenerative signaling pathway for repairing liver injury in vivo [J]. | CHEMICAL COMMUNICATIONS , 2023 , 59 (12) : 1621-1624 . |
| MLA | Liang, Hong et al. "Circular bivalent aptamers enhance the activation of the regenerative signaling pathway for repairing liver injury in vivo" . | CHEMICAL COMMUNICATIONS 59 . 12 (2023) : 1621-1624 . |
| APA | Liang, Hong , Yan, Zhike , Tong, Yuhong , Chen, Shan , Li, Jingying , Chen, Lanlan et al. Circular bivalent aptamers enhance the activation of the regenerative signaling pathway for repairing liver injury in vivo . | CHEMICAL COMMUNICATIONS , 2023 , 59 (12) , 1621-1624 . |
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Helicobacter pylori is closely linked to many gastric diseases such as gastric ulcers and duodenal ulcers. Therefore, biosensing H. pylori has attracted wide attention from both scientists and clinicians. Here, we proposed an electrochemiluminescence (ECL)-based platform that could sensitively detect H. pylori DNA. In this platform, a novel target-cycling synchronized rolling circle amplification was used for signal amplification. Silver nanoclusters (Ag NCs) were synthesized on the circle DNA products, embedding them with the ability to catalyze the electrochemical reduction of K2S2O8, in turn resulting in rapid consumption of the ECL co-reactant near the working electrode, and leading to a decrease in the ECL emission intensity. In addition to its excellent stability and selectivity, the proposed strategy had a low detection limit of 10 pM, an indication that it can be beneficially applied to test biosamples. Furthermore, a biosensing chip was designed to improve the throughput and shed new light on large-scale clinical biosensing applications.
Keyword ：
electrochemiluminescence electrochemiluminescence Helicobacter pylori Helicobacter pylori rolling circle amplification rolling circle amplification
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| GB/T 7714 | Zhang, Xiaorong , Deng, Yuan , Qiu, Hongzhao et al. Target-cycling synchronized rolling circle amplification strategy for biosensing Helicobacter pylori DNA [J]. | LUMINESCENCE , 2023 , 38 (3) : 334-340 . |
| MLA | Zhang, Xiaorong et al. "Target-cycling synchronized rolling circle amplification strategy for biosensing Helicobacter pylori DNA" . | LUMINESCENCE 38 . 3 (2023) : 334-340 . |
| APA | Zhang, Xiaorong , Deng, Yuan , Qiu, Hongzhao , Yi, Sirui , Huang, Sijia , Chen, Lanlan et al. Target-cycling synchronized rolling circle amplification strategy for biosensing Helicobacter pylori DNA . | LUMINESCENCE , 2023 , 38 (3) , 334-340 . |
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Small molecule aptamers discovered by traditional selection methods usually lack conformational changes upon target binding. This limits the use of aptamers as molecular probes for small molecule detection and regulatory elements of genetic circuits. Here, we report a new method called capture and in vitro transcription-systematic evolution of ligands by exponential enrichment (CIVT-SELEX) to select DNA aptamers that can not only bind to small molecule ligands but also undergo significant conformational changes. Through this method, we select a structure-switching aptamer of uridine-5 '-diphosphate (UDP). Taking advantage of its conformational changes, we first construct a UDP-responsive transcriptional switch by inserting the aptamer in a genetic circuit and demonstrate that it can respond to the addition of UDP and regulate the transcription of downstream genes. We also build a UDP aptamer-based biosensor that can be used for active glycosyltransferase screening. We believe this method can provide a universal platform for selecting small molecule aptamers with conformational changes and expand the use of aptamers in small molecule detection and genetic regulation.
Keyword ：
aptamers aptamers biosensors biosensors CIVT-SELEX CIVT-SELEX conformational change conformational change small molecule small molecule
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| GB/T 7714 | Guo, Shaobin , Lin, Jingjing , Lin, Lujie et al. Selecting small molecule DNA aptamers with significant conformational changes for constructing transcriptional switches and biosensors [J]. | SCIENCE CHINA-CHEMISTRY , 2023 , 66 (5) : 1529-1536 . |
| MLA | Guo, Shaobin et al. "Selecting small molecule DNA aptamers with significant conformational changes for constructing transcriptional switches and biosensors" . | SCIENCE CHINA-CHEMISTRY 66 . 5 (2023) : 1529-1536 . |
| APA | Guo, Shaobin , Lin, Jingjing , Lin, Lujie , Xu, Wen , Guo, Yan , Xu, Zipeng et al. Selecting small molecule DNA aptamers with significant conformational changes for constructing transcriptional switches and biosensors . | SCIENCE CHINA-CHEMISTRY , 2023 , 66 (5) , 1529-1536 . |
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As a crucial indicator in food and water safety testing, the detection of Escherichia coli plays a significant role in maintaining environmental sanitation and promoting public health. Herein, based on the electrochemical activity characteristics of E. coli, we established an enhanced electrochemiluminescence aptasensor for E. coli analysis. This study presents a new method for accurate identification by utilizing a double aptamer recognition system. Specifically, a nano-cadmium sulfide (CdS) modified aptamer was used for primary labeling, while a second aptamer was immobilized on a graphene/chitosan composite electrode for re-capture. The use of two aptamers improves the accuracy of the identification process. Furthermore, the application of an electrode potential facilitates continuous electron transfer between the electrode and electrochemically active microorganisms, resulting in an enhanced electroluminescence signal in relation to the metabolic status. This strategy possesses better sensitivity, accuracy, and stability, demonstrating its potential for E. coli analysis.
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| GB/T 7714 | Zhong, Xinyi , Deng, Yuan , Yang, Qiling et al. An extracellular electron transfer enhanced electrochemiluminescence aptasensor for Escherichia coli analysis [J]. | ANALYST , 2023 , 148 (18) : 4414-4420 . |
| MLA | Zhong, Xinyi et al. "An extracellular electron transfer enhanced electrochemiluminescence aptasensor for Escherichia coli analysis" . | ANALYST 148 . 18 (2023) : 4414-4420 . |
| APA | Zhong, Xinyi , Deng, Yuan , Yang, Qiling , Yi, Sirui , Qiu, Haiyan , Chen, Lanlan et al. An extracellular electron transfer enhanced electrochemiluminescence aptasensor for Escherichia coli analysis . | ANALYST , 2023 , 148 (18) , 4414-4420 . |
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