Query：
学者姓名：韩金志
Refining：
Year
Type
Indexed by
Source
Complex
Co-
Language
Clean All
Abstract ：
Aims To address the increasingly serious challenge of the transmission of foodbrone pathogens in the food chain.Methods and results In this study, we employed rational design strategies, including truncation, amino acid substitution, and heterozygosity, to generate seven engineered peptides with alpha-helical structure, cationic property, and amphipathic characteristics based on the original Abhisin template. Among them, as the hybird antimicrobial peptide (AMP), AM exhibits exceptional stability, minimal toxicity, as well as broad-spectrum and potent antimicrobial activity against foodborne pathogens. Besides, it was observed that the electrostatic incorporation demonstrates by AM results in its primary targeting and disruption of the cell wall and membrane of Escherichia coli O157: H7 (EHEC) and methicillin-resistant Staphylococcus aureus (MRSA), resulting in membrane perforation and enhanced permeability. Additionally, AM effectively counteracts the deleterious effects of lipopolysaccharide, eradicating biofilms and ultimately inducing the demise of both food spoilage and pathogenic microorganisms.Conclusions The findings highlight the significant potential of AM as a highly promising candidate for a novel food preservative and its great importance in the design and optimization of AMP-related agents.
Keyword ：
Abhisin Abhisin EHEC EHEC engineered peptides engineered peptides MRSA MRSA rational design rational design
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Han, Jinzhi , Wu, Peifen , Yang, Jie et al. Development of a novel hybrid antimicrobial peptide for enhancing antimicrobial spectrum and potency against food-borne pathogens [J]. | JOURNAL OF APPLIED MICROBIOLOGY , 2024 , 135 (2) . |
| MLA | Han, Jinzhi et al. "Development of a novel hybrid antimicrobial peptide for enhancing antimicrobial spectrum and potency against food-borne pathogens" . | JOURNAL OF APPLIED MICROBIOLOGY 135 . 2 (2024) . |
| APA | Han, Jinzhi , Wu, Peifen , Yang, Jie , Weng, Yanlin , Lin, Yayi , Chen, Zhiying et al. Development of a novel hybrid antimicrobial peptide for enhancing antimicrobial spectrum and potency against food-borne pathogens . | JOURNAL OF APPLIED MICROBIOLOGY , 2024 , 135 (2) . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
Background: The bacteriocins, particularly derived from lactic acid bacteria, currently exhibit potential as a promising food preservative owing to their low toxicity and potent antimicrobial activity. This study aimed to evaluate the efficacy of lactocin 63, produced by Lactobacillus coryniformis, in inhibiting the deterioration of Lateolabrax japonicas during chilled storage, while also investigating its underlying inhibitory mechanism. The measurement of total viable count, biogenic amines, and volatile organic compounds were conducted, along with high-throughput sequencing and sensory evaluation. Results: The findings demonstrated that treatment with lactocin 63 resulted in a notable retardation of bacterial growth in L. japonicas fish fillet during refrigerated storage compared with the water-treated and nisin-treated groups. Moreover, lactocin 63 effectively maintained the microbial flora balance in the fish fillet and inhibited the proliferation and metabolic activity of specific spoilage microorganisms, particularly Shewanella, Pseudomonas, and Acinetobacter. Furthermore, the production of unacceptable volatile organic compounds (e.g. 1-octen-3-ol, hexanal, nonanal), as well as the biogenic amines derived from the bacterial metabolism, could be hindered, thus preventing the degradation in the quality of fish fillets and sustaining relatively high sensory quality. Conclusion: The results of this study provide valuable theoretical support for the development and application of lactocin 63, or other bacteriocins derived from lactic acid bacteria, as potential bio-preservatives in aquatic food. (c) 2024 Society of Chemical Industry.
Keyword ：
bacteriocin bacteriocin lactocin 63 lactocin 63 Lateolabrax japonicus Lateolabrax japonicus microbial community microbial community preservation effects preservation effects seabass seabass
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Wu, Peifen , Yang, Jie , Meng, Xiaojie et al. The inhibitory action of lactocin 63 on deterioration of seabass (Lateolabrax japonicus) during chilled storage [J]. | JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE , 2024 , 104 (7) : 4015-4027 . |
| MLA | Wu, Peifen et al. "The inhibitory action of lactocin 63 on deterioration of seabass (Lateolabrax japonicus) during chilled storage" . | JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE 104 . 7 (2024) : 4015-4027 . |
| APA | Wu, Peifen , Yang, Jie , Meng, Xiaojie , Weng, Yanlin , Lin, Yayi , Li, Ruili et al. The inhibitory action of lactocin 63 on deterioration of seabass (Lateolabrax japonicus) during chilled storage . | JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE , 2024 , 104 (7) , 4015-4027 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
Hongqu rice wine was brewed with glutinous rice as raw material and Hongqu as starter. Monascus and Saccharomyces cerevisiae were the core microorganisms in the brewing system. In this paper, using glutinous rice as the fermentation substrate, Monascus puijmreus, Monascus ruber and Monascus kaoliang were combined with S. cerevisiae to study the effects of different combinations and fermentation modes (simultaneous fermentation and sequential fermentation) on the production of volatile components. A total of 89 volatile flavor compounds were identified from fermented rice wine based on headspace solid phase microextraction-gas chromatography mass spectrometry (HS-SPME-GC-MS). Heat map analysis showed that the combined fermentation of Monascus and S. cerevisiae produced more aroma substances than the pure fermentation of Monascus, and the sequential fermentation produced more aroma substances than the simultaneous fermentation, especially the sequential fermentation of M. purpureas, M. ruber and S. cerevuiae. According to the principal component analysis of volatile flavor components of different Monascus and S. cerevisiae combined fermentation, M. purpureus and M. ruber and S. cerevisiae will produce more volatile flavor components under the sequential fermentation mode, and their flavor compositions were close to each other. Through the analysis of the content difference of volatile flavor components, isobutanol, 1-heptanol, (3Z)-3-nonene-1-ol, 2-tetradecanol, (Z)-5-octene-1-ol, decanal, ethyl caproate, ethyl decanoate, 9 -decenoate, ethyl phenylacetate, ethyl acetate, 1,2 -dimethoxy -4 -vinyl benzene, acridine were the characteristic volatile flavor substances in the combined fermentation products of Monascus and S. cerevisiae. The results of this study could provide a theoretical basis for the improvement of flavor quality and quality control in the industrial production of Hongqu rice wine. © 2024 Chinese Institute of Food Science and Technology. All rights reserved.
Keyword ：
Fermentation Fermentation Flavor compounds Flavor compounds Gas chromatography Gas chromatography Mass spectrometry Mass spectrometry Odors Odors Principal component analysis Principal component analysis Quality control Quality control Substrates Substrates Volatile organic compounds Volatile organic compounds Wine Wine Yeast Yeast
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | You, Wenqiang , Yan, Yingyin , Yang, Ziyi et al. Effects of Combinations of Monascus and Saccharomyces cerevisiae on the Formation of Volatile Flavor Components of Rice Wine [J]. | Journal of Chinese Institute of Food Science and Technology , 2024 , 24 (3) : 287-297 . |
| MLA | You, Wenqiang et al. "Effects of Combinations of Monascus and Saccharomyces cerevisiae on the Formation of Volatile Flavor Components of Rice Wine" . | Journal of Chinese Institute of Food Science and Technology 24 . 3 (2024) : 287-297 . |
| APA | You, Wenqiang , Yan, Yingyin , Yang, Ziyi , Sun, Jinyuan , Zhang, Wen , Han, Jinzhi et al. Effects of Combinations of Monascus and Saccharomyces cerevisiae on the Formation of Volatile Flavor Components of Rice Wine . | Journal of Chinese Institute of Food Science and Technology , 2024 , 24 (3) , 287-297 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
该文以鲜榨豆薯汁为植物乳杆菌FZU122发酵基质，探究发酵时间（0、24、48、72 h）对豆薯汁基本理化指标、游离单糖、游离氨基酸、有机酸和挥发性成分的影响。结果表明：pH值随发酵时间的延长显著下降。发酵48 h后，豆薯汁中总还原糖浓度显著变化，葡萄糖和果糖为主要的单糖成分。总游离氨基酸的含量在发酵后出现波动，其中苦味氨基酸含量下降，甜味氨基酸含量上升。气相色谱-质谱联用技术检测发现，醇类物质为发酵豆薯汁的主要香气组分，原有的醛类物质发酵后未被检出。发酵后总有机酸含量升高，苹果酸和乳酸为豆薯中两种主要酸类物质。
Keyword ：
挥发性成分 挥发性成分 有机酸 有机酸 益生菌发酵 益生菌发酵 豆薯汁 豆薯汁 还原糖 还原糖
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | 俞曼玲 , 黄晶晶 , 韩金志 et al. 植物乳杆菌发酵豆薯的营养及挥发性成分变化 [J]. | 食品研究与开发 , 2023 , 44 (17) : 61-67 . |
| MLA | 俞曼玲 et al. "植物乳杆菌发酵豆薯的营养及挥发性成分变化" . | 食品研究与开发 44 . 17 (2023) : 61-67 . |
| APA | 俞曼玲 , 黄晶晶 , 韩金志 , 汪少芸 , 施晓丹 . 植物乳杆菌发酵豆薯的营养及挥发性成分变化 . | 食品研究与开发 , 2023 , 44 (17) , 61-67 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
经乳酸菌发酵可显著改善果蔬汁饮料的风味，并提高产品营养价值。文章以芒果、哈密瓜复合果汁为发酵基质，通过植物乳植杆菌发酵，并对不同发酵时间果汁的pH、挥发性香气成分的变化过程以及感官品质进行测定。结果表明，果汁经发酵36 h后，pH值下降至3.5，并保持恒定至发酵结束；同时，随着发酵过程的进展，复合果汁中的醛、醇、酸、酯等物质均呈现逐步积累趋势；此外，不同发酵时间复合果汁的香气组成有较大差异，其中发酵36 h时果汁中的挥发性气味物质达最多，主要特征化合物为2-庚酮、2-乙基己醇、甲酸己酯、1-石竹烯、2-壬酮等，可进一步赋予发酵果汁花香、果香和酯香。同时，感官评价结果表明，经乳植杆菌发酵36 h复合果汁的感官品质亦达到最佳。
Keyword ：
GC-MS GC-MS 乳植杆菌发酵 乳植杆菌发酵 复合果汁饮料 复合果汁饮料 香气物质 香气物质
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | 陈驰 , 吴伯文 , 杨晓丽 et al. 植物乳植杆菌发酵复合果汁饮料香气成分变化过程的分析 [J]. | 福建轻纺 , 2023 , 6 (08) : 7-11,80 . |
| MLA | 陈驰 et al. "植物乳植杆菌发酵复合果汁饮料香气成分变化过程的分析" . | 福建轻纺 6 . 08 (2023) : 7-11,80 . |
| APA | 陈驰 , 吴伯文 , 杨晓丽 , 韩金志 , 周红艳 . 植物乳植杆菌发酵复合果汁饮料香气成分变化过程的分析 . | 福建轻纺 , 2023 , 6 (08) , 7-11,80 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
The widespread and escalating emergence of multidrug resistance is now recognized as one of the most severe global threats to human health. To address the urgent issue of drug-resistant bacteria and the limitation of effective clinical treatments, antimicrobial peptides (AMPs) have been developed as promising substituents of conventional antibiotics. In this study, rational design strategies were employed to acquire seven cationic and alpha-helical engineered peptides based on the original template of Abaecin. After investigation, we found that AC7 (LLRRWKKLFKKIIRWPRPLPNPGH) demonstrated potent and broad-spectrum antimicrobial activity. Additionally, it demonstrated low cytotoxicity and hemolysis while maintaining good stability. Notably, AC7 displays the antibacterial mechanism with superior abilities in cell membrane disruption and potential DNA binding in vitro, as well as effectively disrupting biofilms. Moreover, the murine skin wound model infected with drug-resistant Pseudomonas aeruginosa was employed to evaluate the anti-infective efficacy and therapeutic potential of AC7. It was observed that AC7 displays a remarkable capacity to inhibit wound colonization, reduce levels of inflammatory cytokines (TNF-alpha) and inflammatory cells (white blood cells (WBC), monocytes (MONO), lymphocytes (LYMPH), neutrophils (GRAN)), promote the levels of IL-10 and VEGF, and enhance wound healing. Overall, these findings demonstrate the potential of AC7 as a viable alternative to traditional antibiotics.
Keyword ：
abaecin abaecin AC7 AC7 antimicrobialactivity antimicrobialactivity murine skin wound infection model murine skin wound infection model rational design rational design
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Yang, Jie , Wu, Peifen , Weng, Yanlin et al. Rational Design and Antimicrobial Potency Assessment of Abaecin Analogues [J]. | ACS BIOMATERIALS SCIENCE & ENGINEERING , 2023 , 9 (12) : 6698-6714 . |
| MLA | Yang, Jie et al. "Rational Design and Antimicrobial Potency Assessment of Abaecin Analogues" . | ACS BIOMATERIALS SCIENCE & ENGINEERING 9 . 12 (2023) : 6698-6714 . |
| APA | Yang, Jie , Wu, Peifen , Weng, Yanlin , Lin, Yayi , Chen, Zhiying , Yu, Fengfan et al. Rational Design and Antimicrobial Potency Assessment of Abaecin Analogues . | ACS BIOMATERIALS SCIENCE & ENGINEERING , 2023 , 9 (12) , 6698-6714 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
Infections caused by pathogens can be a significant challenge in wound healing, particularly when antimicrobial resistance is a factor. This can pose a serious threat to human health and well-being. In this scenario, it is imperative to explore novel antimicrobial agents to fight against multi-drug resistant (MDR) pathogenic bacteria. This study employed rational design strategies, including truncation, amino acid replacement, and heterozygosity, to obtain seven a-helical, cationic, and engineered peptides based on the original template of Abhisin. Among the analogs of Abhisin, AB7 displayed broad-spectrum and potent antimicrobial activity, superior targeting of membranes and DNA, and the ability to disrupt biofilms and anti-endotoxins in vitro. Additionally, we evaluated the anti-infection ability of AB7 using a murine skin wound model infected with methicillin-resistant Staphylococcus aureus (MRSA) and found that AB7 displayed negligible toxicity both in vitro and in vivo. Furthermore, AB7 exhibited desirable therapeutic efficacy by reducing bacterial burden and pro-inflammatory mediators, modulating cytokines, promoting wound healing, and enhancing angiogenesis. These results highlight the potential of AB7 as a promising candidate for a new antibiotic.
Keyword ：
Abhisin-like peptides Abhisin-like peptides Antimicrobial activity Antimicrobial activity Arginine-rich peptides Arginine-rich peptides MRSA infections MRSA infections Murine wound model Murine wound model
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Wu, Peifen , Yang, Jie , Chen, Chi et al. Rational design of Abhisin-like peptides enables generation of potent antimicrobial activity against pathogens [J]. | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY , 2023 , 107 (21) : 6621-6640 . |
| MLA | Wu, Peifen et al. "Rational design of Abhisin-like peptides enables generation of potent antimicrobial activity against pathogens" . | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 107 . 21 (2023) : 6621-6640 . |
| APA | Wu, Peifen , Yang, Jie , Chen, Chi , Li, Ruili , Chen, Shunxian , Weng, Yanlin et al. Rational design of Abhisin-like peptides enables generation of potent antimicrobial activity against pathogens . | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY , 2023 , 107 (21) , 6621-6640 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
本发明公开了一种广谱抗菌肽CA‑1，其氨基酸序列为GLLSVLGSVAKHVLPHVVPVIAEHLWKKLFKK‑NH2。该广谱抗菌肽是结合现有的抗菌肽相关理论知识，对澳大利亚树蛙来源的天然抗菌肽Caerin1.1通过理性分子设计进行改造而得到的。抑菌实验结果表明，本发明的抗菌肽CA‑1具有广谱的抑菌效果，其对革兰氏阳性菌、革兰氏阴性菌均有很好的抑制效果，抑菌浓度可达1~8μg/ml。经圆二色谱测定，抗菌肽CA‑1在205~220nm处有负值吸收峰，在TFE溶液中可形成α螺旋结构。
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | 汪少芸 , 韩金志 , 杨捷 et al. 一种基于理性设计策略构建的广谱抗菌肽 : CN202210098946.0[P]. | 2022-01-27 00:00:00 . |
| MLA | 汪少芸 et al. "一种基于理性设计策略构建的广谱抗菌肽" : CN202210098946.0. | 2022-01-27 00:00:00 . |
| APA | 汪少芸 , 韩金志 , 杨捷 , 武培汾 , 陈舜娴 , 陈选 et al. 一种基于理性设计策略构建的广谱抗菌肽 : CN202210098946.0. | 2022-01-27 00:00:00 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
Multidrug-resistant bacteria infections frequently occur in wound care due to the excessive use of antibiotics. It can cause scar formation, wound closure delay, multiple organ failure, and high mortality. Here, a double network hydrogel with injectability, hemostasis, and antibacterial activity was developed to prompt multidrug-resistant bacteria infected wound healing. The double network hydrogel is composed of gelatin methacryloyl (GelMA), oxidized dextran (ODex), epsilon-polylysine (EPL), and bacitracin, and formed through the Schiff-base and UV-initiated crosslinking reaction. The injectable hydrogel with an adhesion effect could adapt to the irregular shape of the wound and possesses good hemostatic ability. The hydrogel presents good flexibility and rapid resilience due to its double network structure, and it can prompt cell proliferation and migration. In particular, the hydrogel has broad-spectrum in vitro antimicrobial activities against S. aureus, E. coli, and methicillin-resistant S. aureus (MRSA), and disrupts E. coli and MRSA biofilms. In vivo results demonstrated that the hydrogel can completely heal MRSA-infected wound in rats within 15 days, through inhibiting the growth of bacteria, accelerating skin tissue reepithelialization, collagen deposition, and angiogenesis, as well as adjusting the expression of CD31, alpha-SMA, and TNF-alpha. The findings of this study suggest that the presented hydrogel could enhance multidrug-resistant bacteria infected wound healing and mitigate antimicrobial resistance.
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Zhang, Zibo , Guo, Jiadong , He, Yuxiang et al. An injectable double network hydrogel with hemostasis and antibacterial activity for promoting multidrug-resistant bacteria infected wound healing [J]. | BIOMATERIALS SCIENCE , 2022 , 10 (12) : 3268-3281 . |
| MLA | Zhang, Zibo et al. "An injectable double network hydrogel with hemostasis and antibacterial activity for promoting multidrug-resistant bacteria infected wound healing" . | BIOMATERIALS SCIENCE 10 . 12 (2022) : 3268-3281 . |
| APA | Zhang, Zibo , Guo, Jiadong , He, Yuxiang , Han, Jinzhi , Chen, Mingmao , Zheng, Yunquan et al. An injectable double network hydrogel with hemostasis and antibacterial activity for promoting multidrug-resistant bacteria infected wound healing . | BIOMATERIALS SCIENCE , 2022 , 10 (12) , 3268-3281 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
Alcoholic liver injury is mainly caused by excessive alcohol consumption and has become a global public health problem threatening human health. It is well known that Ganoderma lucidum possesses various excellent bene-ficial effects on liver function and lipid metabolism. The purpose of this study was to evaluate the underlying protective effect and action mechanism of ganoderic acids-rich G. lucidum ethanol extract (GLE) on alcohol-induced liver injury in mice with excessive alcohol intake. Results showed that oral administration of GLE could obviously inhibit the abnormal increases of serum triglyceride (TG), total cholesterol (TC), low density lipoprotein cholesterol (LDL-C), aspartate aminotransferase (AST) and alanine aminotransferase (ALT), and also significantly protect the liver against alcohol-induced excessive hepatic lipid accumulation and pathological changes. In addition, alcohol-induced oxidative stress in liver was significantly ameliorated by the dietary intervention of GLE through reducing the hepatic levels of maleic dialdehyde (MDA) and lactate dehydrogenase (LDH), and increasing the hepatic levels of glutathione (GSH), catalase (CAT), superoxide dismutase (SOD) and alcohol dehydrogenase (ADH). Compared with the model group, GLE intervention significantly ameliorated the intestinal microbial disorder by elevating the relative abundance of Ruminiclostridium_9, Prevotellaceae_UCG-001, Oscillibacter, [Eubacterium]_xylanophilum_group, norank_f_Clostridiates_vadinBB60_group, GCA-900066225, Bilophila, Ruminococcaceae_UCG-009, norank_f_Desulfovibrionaceae and Hydrogenoanaerobacterium, but decreasing the proportion of Clostridium_sensu_stricto_1. Furthermore, liver metabolomic profiling suggested that GLE intervention had a significant regulatory effect on the composition of liver metabolites in mice with excessive alcohol intake, especially the levels of some biomarkers involved in primary bile acid biosynthesis, riboflavin metabolism, tryptophan metabolism, biosynthesis of unsaturated fatty acids, fructose and mannose metabolism, glycolysis/gluconeogenesis. Additionally, dietary supplementation with GLE significantly regulated the mRNA levels of key genes related to fatty acids metabolism, ethanol catabolism and inflammatory response in liver. Conclusively, these findings indicate that GLE has a potentially beneficial effect on alleviating alcohol-induced liver injury and may be developed as a promising functional food ingredient.
Keyword ：
alcoholic liver injury alcoholic liver injury Ganoderma lucidum Ganoderma lucidum Intestinal microbiota Intestinal microbiota Liver metabolomics Liver metabolomics Triterpenoids Triterpenoids
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Guo, Wei-Ling , Cao, Ying-Jia , You, Shi-Ze et al. Ganoderic acids-rich ethanol extract from Ganoderma lucidum protects against alcoholic liver injury and modulates intestinal microbiota in mice with excessive alcohol intake [J]. | CURRENT RESEARCH IN FOOD SCIENCE , 2022 , 5 : 515-530 . |
| MLA | Guo, Wei-Ling et al. "Ganoderic acids-rich ethanol extract from Ganoderma lucidum protects against alcoholic liver injury and modulates intestinal microbiota in mice with excessive alcohol intake" . | CURRENT RESEARCH IN FOOD SCIENCE 5 (2022) : 515-530 . |
| APA | Guo, Wei-Ling , Cao, Ying-Jia , You, Shi-Ze , Wu, Qi , Zhang, Fang , Han, Jin-Zhi et al. Ganoderic acids-rich ethanol extract from Ganoderma lucidum protects against alcoholic liver injury and modulates intestinal microbiota in mice with excessive alcohol intake . | CURRENT RESEARCH IN FOOD SCIENCE , 2022 , 5 , 515-530 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Export
| Results： |
Selected to |
| Format： |
