Query：
学者姓名：吕鹏
Refining：
Year
Type
Indexed by
Source
Complex
Co-
Language
Clean All
Abstract ：
Soft-tissue sarcomas (STS) emerges as formidable challenges in clinics due to the complex genetic heterogeneity, high rates of local recurrence and metastasis. Exploring specific targets and biomarkers would benefit the prognosis and treatment of STS. Here, we identified RCC1, a guanine-nucleotide exchange factor for Ran, as an oncogene and a potential intervention target in STS. Bioinformatics analysis indicated that RCC1 is highly expressed and correlated with poor prognosis in STS. Functional studies showed that RCC1 knockdown significantly inhibited the cell cycle transition, proliferation and migration of STS cells in vitro, and the growth of STS xenografts in mice. Mechanistically, we identified Skp2 as a downstream target of RCC1 in STS. Loss of RCC1 substantially diminished Skp2 abundance by compromising its protein stability, resulting in the upregulation of p27Kip1 and G1/S transition arrest. Specifically, RCC1 might facilitate the nucleo-cytoplasmic trafficking of Skp2 via direct interaction. As a result, the cytoplasmic retention of Skp2 would further protect it from ubiquitination and degradation. Notably, recovery of Skp2 expression largely reversed the phenotypes induced by RCC1 knockdown in STS cells. Collectively, this study unveils a novel RCC1-Skp2-p27Kip1 axis in STS oncogenesis, which holds promise for improving prognosis and treatment of this formidable malignancy.
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Zhuang, Mingzhi , Li, Fengyue , Liang, Hong et al. Targeting RCC1 to block the human soft-tissue sarcoma by disrupting nucleo-cytoplasmic trafficking of Skp2 [J]. | CELL DEATH & DISEASE , 2024 , 15 (4) . |
| MLA | Zhuang, Mingzhi et al. "Targeting RCC1 to block the human soft-tissue sarcoma by disrupting nucleo-cytoplasmic trafficking of Skp2" . | CELL DEATH & DISEASE 15 . 4 (2024) . |
| APA | Zhuang, Mingzhi , Li, Fengyue , Liang, Hong , Su, Yongfu , Cheng, Lei , Lin, Bingkai et al. Targeting RCC1 to block the human soft-tissue sarcoma by disrupting nucleo-cytoplasmic trafficking of Skp2 . | CELL DEATH & DISEASE , 2024 , 15 (4) . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
Hepatocellular carcinoma (HCC) poses a significant threat due to its highly aggressive and high recurrence characteristics, necessitating urgent advances in diagnostic and therapeutic approaches. Long non-coding RNAs exert vital roles in HCC tumorigenesis, however the mechanisms of their expression regulation and functions are not fully elucidated yet. Herein, we identify that a novel tumor suppressor 'lnc-PIK3R1' was significantly downregulated in HCC tissues, which was correlated with poor prognosis. Functionally, lnc-PIK3R1 played tumor suppressor roles to inhibit the proliferation and mobility of HCC cells, and to impede the distant implantation of xenograft in mice. Mechanistic studies revealed that lnc-PIK3R1 interacted with miR-1286 and alleviated the repression on GSK3B by miR-1286. Notably, pharmacological inhibition of GSK3 beta compromised the tumor suppression effect by lnc-PIK3R1, confirming their functional relevance. Moreover, we identified that oncogenic YY1 acts as a specific transcriptional repressor to downregulate the expression of lnc-PIK3R1 in HCC. In summary, this study highlights the tumor-suppressive effect of lnc-PIK3R1, and provides new insights into the regulation of GSK3 beta expression in HCC, which would benefit the development of innovative intervention strategies for HCC.
Keyword ：
GSK3 beta GSK3 beta Hepatocellular carcinoma (HCC) Hepatocellular carcinoma (HCC) miR-1286 miR-1286
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Lyu, Peng , Li, Fengyue , Deng, Runzhi et al. Lnc-PIK3R1, transcriptionally suppressed by YY1, inhibits hepatocellular carcinoma progression via the Lnc-PIK3R1/miR-1286/GSK3β axis [J]. | BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE , 2024 , 1870 (6) . |
| MLA | Lyu, Peng et al. "Lnc-PIK3R1, transcriptionally suppressed by YY1, inhibits hepatocellular carcinoma progression via the Lnc-PIK3R1/miR-1286/GSK3β axis" . | BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE 1870 . 6 (2024) . |
| APA | Lyu, Peng , Li, Fengyue , Deng, Runzhi , Wei, Qiliang , Lin, Bingkai , Cheng, Lei et al. Lnc-PIK3R1, transcriptionally suppressed by YY1, inhibits hepatocellular carcinoma progression via the Lnc-PIK3R1/miR-1286/GSK3β axis . | BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE , 2024 , 1870 (6) . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
A simple and feasible pH meter-based immunoassay is reported for detection of C-reactive protein (CRP) using glucose oxidase (GOD)-conjugated dendrimer loaded with platinum nanozyme. Initially, platinum nanozymes were loaded into the dendrimers through an in situ synthetic method. Then, GOD and monoclonal anti-CRP antibody with a high molar ratio were covalently conjugated onto carboxylated dendrimers via typical carbodiimide coupling. The immunoreaction was carried out with a competitive mode in a CRP-coated microplate. Along with formation of immunocomplex, the added glucose was oxidized into gluconic acid and hydrogen peroxide by GOD, and the latter was further decomposed by platinum nanozyme, thus accelerating chemical reaction in the positive direction. The produced gluconic acid changed the pH of detection solution, which was determined using a handheld pH meter. Under optimum conditions, the pH meter-based immunoassay gave a good signal toward target CRP from 0.01 to 100 ng mL(-1). The limit of detection was 5.9 pg mL(-1). An intermediate precision <= 11.2% was acquired with batch-to-batch identification. No nonspecific adsorption was observed during a series of procedures to detect target CRP, and the cross-reaction against other biomarkers was very low. Importantly, our system gave well-matched results for analysis of human serum samples relative to a referenced ELISA kit.Graphical abstract
Keyword ：
C-reactive protein C-reactive protein Enzymatic cascade amplification Enzymatic cascade amplification Nanoparticle-encapsulated dendrimer Nanoparticle-encapsulated dendrimer pH detection pH detection Platinum nanozyme Platinum nanozyme Potentiometric immunoassay Potentiometric immunoassay
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Li, Bin , Ge, Lilin , Lyu, Peng et al. Handheld pH meter-assisted immunoassay for C-reactive protein using glucose oxidase-conjugated dendrimer loaded with platinum nanozymes [J]. | MICROCHIMICA ACTA , 2021 , 188 (1) . |
| MLA | Li, Bin et al. "Handheld pH meter-assisted immunoassay for C-reactive protein using glucose oxidase-conjugated dendrimer loaded with platinum nanozymes" . | MICROCHIMICA ACTA 188 . 1 (2021) . |
| APA | Li, Bin , Ge, Lilin , Lyu, Peng , Chen, Meijuan , Zhang, Xiongfei , Xie, Shuping et al. Handheld pH meter-assisted immunoassay for C-reactive protein using glucose oxidase-conjugated dendrimer loaded with platinum nanozymes . | MICROCHIMICA ACTA , 2021 , 188 (1) . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
Loss of neuron homeostasis in the arcuate nucleus (ARC) is responsible for diet-induced-obesity (DIO). We previously reported that loss of Rb1 gene compromised the homeostasis of anorexigenic POMC neurons in ARC and induced obesity in mice. To evaluate the development of DIO, we propose to analyze the transcriptomic alteration of POMC neurons in mice following high fat diet (HFD) feeding. We isolated these neurons from established DIO mice and performed transcriptomic profiling using RNA-seq. In total, 1066 genes (628 upregulated and 438 downregulated) were identified as differentially expressed genes (DEGs). Pathway enrichment analysis with these DEGs further revealed that "cell cycle," "apoptosis," "chemokine signaling," and "sphingo-lipid metabolism" pathways were correlated with DIO development. Moreover, we validated that the pRb protein, a key regulator of "cell cycle pathway," was inactivated by phosphorylation in POMC neurons by HFD feeding. Importantly, the reversal of deregulated cell cycle by stereotaxic delivering of the unphosphorylated pRb.P in ARC significantly meliorated the DIO. Collectively, our study provides insights into the mechanisms related to the loss of homeostasis of POMC neurons in DIO, and suggests pRb phosphorylation as a potential intervention target to treat DIO.
Keyword ：
Diet-induced obesity (DIO) Diet-induced obesity (DIO) High-fat-diet (HFD) High-fat-diet (HFD) Neuron homeostasis Neuron homeostasis POMC neuron POMC neuron pRb phosphorylation pRb phosphorylation
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Lyu, Peng , Huang, Zhishun , Feng, Qingjun et al. Unveiling the transcriptome alteration of POMC neuron in diet-induced obesity [J]. | EXPERIMENTAL CELL RESEARCH , 2020 , 389 (1) . |
| MLA | Lyu, Peng et al. "Unveiling the transcriptome alteration of POMC neuron in diet-induced obesity" . | EXPERIMENTAL CELL RESEARCH 389 . 1 (2020) . |
| APA | Lyu, Peng , Huang, Zhishun , Feng, Qingjun , Su, Yongfu , Zheng, Mengying , Hong, Yannv et al. Unveiling the transcriptome alteration of POMC neuron in diet-induced obesity . | EXPERIMENTAL CELL RESEARCH , 2020 , 389 (1) . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
A new electrochemical immunoassay was designed for the sensitive detection of prostate-specific antigen (PSA) via a pyrophosphatase (PPase)-hydrolysed Cu(ii)-coordinated pyrophosphate ion (Cu2+-PPi) complex, accompanying the release of Cu(ii) ions and capture on a negatively charged electrode. This system involved a sandwiched immunoreaction with nanogold-labeled PPase/detection antibodies on a polystyrene microplate, enzymatic hydrolysis, Cu(ii) release and voltammetric measurement. The voltammetric signal is derived from the as-released Cu(ii) ions within the applied potential on the electrode. Under optimum conditions, our strategy exhibited a good voltammetric response for the analyte, and allowed determination of PSA at a concentration as low as 5.2 pg mL(-1). Good precision, high specificity and acceptable accuracy were achieved for the detection of PSA.
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Xie, Shuping , Li, Bin , Lyu, Peng et al. A new voltammetric immunosensing platform for prostate-specific antigen based on the Cu(ii)-pyrophosphate ion chelation reaction [J]. | NEW JOURNAL OF CHEMISTRY , 2020 , 44 (10) : 3820-3823 . |
| MLA | Xie, Shuping et al. "A new voltammetric immunosensing platform for prostate-specific antigen based on the Cu(ii)-pyrophosphate ion chelation reaction" . | NEW JOURNAL OF CHEMISTRY 44 . 10 (2020) : 3820-3823 . |
| APA | Xie, Shuping , Li, Bin , Lyu, Peng , Kwok, Hang Fai , Ge, Lilin , Wu, Qinan . A new voltammetric immunosensing platform for prostate-specific antigen based on the Cu(ii)-pyrophosphate ion chelation reaction . | NEW JOURNAL OF CHEMISTRY , 2020 , 44 (10) , 3820-3823 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
Phenylboronic acid-functionalized nanometer-sized CaCO3 particles (PBA-CaCO3) were designed to determine the carcinoembryonic antigen (CEA) glycoprotein with a portable Ca2+ ion-selective electrode (Ca-ISE) through a typical boronate ester bond. CaCO3 nanospheres were conjugated to 3-aminophenylboronic acid by amine-epoxy reaction, whereas target CEA was captured into the aptasensing interface by the immobilized thiolated aptamer on gold substrate. Upon PBA-CaCO3 introduction, 3-aminophenylboronic acid labeled to CaCO3 microsphere specifically recognized with CEA glycoprotein based on sugar-boronic acid interaction to form a sandwiched complex. The carried CaCO3 was dissolved under acidic conditions to release Ca2+ ion with a portable Ca-ISE readout. Thanks to the specific boronate ester bond between PBA and 1,2-diols, the synthesized PBA-CaCO3 exhibited good conjugation properties for CEA glycoprotein. Under optimum conditions, Ca-ISE-based aptasensing platform exhibited good electrode potential response for evaluation of target CEA, and allowed detection of CEA at a concentration as low as 7.3 pg mL(-1). Importantly, Ca-ISE-based aptasensing system is readily extended to detect other disease-related glycoproteins by controlling the corresponding aptamer.
Keyword ：
Aptasensor Aptasensor Carcinoembryonic antigen Carcinoembryonic antigen Ion-selective electrode Ion-selective electrode Nanometer-sized CaCO3 Nanometer-sized CaCO3 Potentiometric measurement Potentiometric measurement
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Chen, Ye , Li, Bin , Lyu, Peng et al. Boronate ester bond-based potentiometric aptasensor for screening carcinoembryonic antigen-glycoprotein using nanometer-sized CaCO3 with ion-selective electrode [J]. | ANALYTICAL AND BIOANALYTICAL CHEMISTRY , 2020 , 413 (4) : 1073-1080 . |
| MLA | Chen, Ye et al. "Boronate ester bond-based potentiometric aptasensor for screening carcinoembryonic antigen-glycoprotein using nanometer-sized CaCO3 with ion-selective electrode" . | ANALYTICAL AND BIOANALYTICAL CHEMISTRY 413 . 4 (2020) : 1073-1080 . |
| APA | Chen, Ye , Li, Bin , Lyu, Peng , Kwok, Hang Fai , Ge, Lilin , Wu, Qinan . Boronate ester bond-based potentiometric aptasensor for screening carcinoembryonic antigen-glycoprotein using nanometer-sized CaCO3 with ion-selective electrode . | ANALYTICAL AND BIOANALYTICAL CHEMISTRY , 2020 , 413 (4) , 1073-1080 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
Amphibians are a natural source of abundant antimicrobial peptides and thus have been widely investigated for isolation of such biomolecules. Many new antimicrobial peptide families have been discovered from amphibians. In this study, a novel antimicrobial peptide named Limnonectes fujianensis Brevinvin (LFB) has been identified in the skin secretion from the Fujian large headed frog, Limnonectes fujianensis. The cDNA sequence was cloned from a skin secretion library and the predicted mature peptide was identified through MS/MS fragmentation sequencing of reverse phase HPLC fractions on the same sample. LFB was predicted to be an amphipathic, hydrophobic, alpha helical, and beta turn peptide that inserts into a lipid bilayer in order to kill the cells. In antimicrobial assays, a synthetic replicate of this novel antimicrobial peptide demonstrated significant activity against the Gram-positive bacterium Staphylococcus aureus, the Gram-negative bacterium Escherichia coli and the yeast, Candida albicans. This novel peptide was highly potent (MIC 4.88 uM) against Gram-negative bacterium, and also has the ability to inhibit the growth of human cancer cell lines with IC50 values ranging from 18.9 mu M down to 2.0 mu M. These findings help to enrich our understanding of Brevinin-like peptides. Moreover, the data presented here validate frog secretion as a source of potential novel antimicrobial peptides, that also exhibit anti-tumor properties, that could be useful for the treatment of cancer.
Keyword ：
amphibian amphibian anticancer peptide anticancer peptide antimicrobial peptides antimicrobial peptides Brevinin-like peptide Brevinin-like peptide drug discovery drug discovery
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Li, Bin , Lyu, Peng , Xie, Shuping et al. LFB: A Novel Antimicrobial Brevinin-Like Peptide from the Skin Secretion of the Fujian Large Headed Frog, Limnonectes fujianensi [J]. | BIOMOLECULES , 2019 , 9 (6) . |
| MLA | Li, Bin et al. "LFB: A Novel Antimicrobial Brevinin-Like Peptide from the Skin Secretion of the Fujian Large Headed Frog, Limnonectes fujianensi" . | BIOMOLECULES 9 . 6 (2019) . |
| APA | Li, Bin , Lyu, Peng , Xie, Shuping , Qin, Haixin , Pu, Wenyuan , Xu, Houxi et al. LFB: A Novel Antimicrobial Brevinin-Like Peptide from the Skin Secretion of the Fujian Large Headed Frog, Limnonectes fujianensi . | BIOMOLECULES , 2019 , 9 (6) . |
| Export to | NoteExpress RIS BibTex |
Version ：
Export
| Results： |
Selected to |
| Format： |
