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学者姓名：王建
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Abstract ：
Changes in the charge density on the inner surface of the microchannel can modulate the ion concentration at the tip, thus causing changes in the resistance of the system. In this study, this property is adopted to construct a portable sensor using a multimeter and aflatoxin B1 (AFB1) is used as the model target. Initially, the cDNA/aptamer complex is modified in the microchannel. The inner microchannel surface's charge density is then altered by the recognition of the target, leading to a change in the system's resistance, which can be conveniently monitored using a multimeter. Critical parameters influencing the performance of the system are optimized. Under optimum conditions, the resistance is linearly related to the logarithm of AFB1 concentration in the range of 100 fM-10 nM and the detection limit is 46 fM (S/N = 3). The resistive measurement is separated from the recognition reaction of the target, reducing the matrix interference during the detection process. This sensor boasts high sensitivity and specificity coupled with commendable reproducibility and stability. It is applied to assay the AFB1 content successfully in an actual sample of corn. Moreover, this approach is cost-effective, user-friendly, and highly accurate.
Keyword ：
aflatoxin B1 aflatoxin B1 microchannel microchannel portablesensor portablesensor resistance resistance surface charge density surface charge density
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| GB/T 7714 | Cai, Huabin , Huang, Yanling , Lin, Yue et al. Portable Sensor for Aflatoxin B1 Based on the Regulation of Resistance of a Microchannel Using a Multimeter as Readout [J]. | ACS SENSORS , 2024 , 9 (1) : 494-501 . |
| MLA | Cai, Huabin et al. "Portable Sensor for Aflatoxin B1 Based on the Regulation of Resistance of a Microchannel Using a Multimeter as Readout" . | ACS SENSORS 9 . 1 (2024) : 494-501 . |
| APA | Cai, Huabin , Huang, Yanling , Lin, Yue , Luo, Fang , Chen, Lifen , Guo, Longhua et al. Portable Sensor for Aflatoxin B1 Based on the Regulation of Resistance of a Microchannel Using a Multimeter as Readout . | ACS SENSORS , 2024 , 9 (1) , 494-501 . |
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The electrochemiluminescence (ECL) intensity can be regulated by ionic current passing through the microchannel, which broadened the regulation of the ECL sensors. But in the early reported sensors, the electrostatic repulsion and steric hindrance caused few targets to approach the interface of the microchannel driven by concentration difference, which reduced the detection efficiency and prolonged the detection period. In this study, different accumulation strategies, such as a positive electric field and different polarity electric fields, were designed to accumulate targets in the microchannel. The interaction of azide groups and hydrogen sulfide served as a research model. Hydrogen sulfide can react with the negatively charged azide groups in the microchannel surface to produce positively charged amino groups, decreasing the negative charge density of the microchannel and thus altering the ionic current and ECL intensity. The accumulation of hydrogen sulfide at the microchannel tip can increase the collision probability with azide groups to improve the detection efficiency, and the integration of accumulation and reaction can shorten the detection period to 28 min. The hydrogen sulfide concentration on the microchannel tip accumulated by applying different polarity electric fields was 22.3-fold higher than that accumulated by applying a positive electric field. The selected research model broadened the application range of a microchannel-based ECL sensor and confirmed the universality of the microchannel-based ECL sensor.
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| GB/T 7714 | Huang, Yanling , Cai, Huabin , Lin, Yue et al. Charge Density-Regulated Microchannel-Based Electrochemiluminescence Sensor for Hydrogen Sulfide Detection with a Highly Efficient Accumulation Strategy [J]. | ANALYTICAL CHEMISTRY , 2024 , 96 (13) : 5251-5257 . |
| MLA | Huang, Yanling et al. "Charge Density-Regulated Microchannel-Based Electrochemiluminescence Sensor for Hydrogen Sulfide Detection with a Highly Efficient Accumulation Strategy" . | ANALYTICAL CHEMISTRY 96 . 13 (2024) : 5251-5257 . |
| APA | Huang, Yanling , Cai, Huabin , Lin, Yue , Luo, Fang , Lin, Cuiying , Wang, Jian et al. Charge Density-Regulated Microchannel-Based Electrochemiluminescence Sensor for Hydrogen Sulfide Detection with a Highly Efficient Accumulation Strategy . | ANALYTICAL CHEMISTRY , 2024 , 96 (13) , 5251-5257 . |
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A novel photoelectrochemical sensor, employing an S-scheme heterojunction of phthalocyanine and TiO2 nanoparticles, has been developed to enable highly sensitive determination of glutathione. By integrating the favorable stability, environmental benignity, and electronic properties of the TiO2 matrix with the unique photoactivity of phthalocyanine species, the designed sensor presents a substantial linear dynamic range and a low detection limit for the quantification of glutathione. The sensitivity is attributed to efficient charge transfer and separation across the staggered heterojunction energy levels, which generates measurable photocurrent signals. Systematic variation of phthalocyanine content reveals an optimal composition that balances light harvesting capacity and electron-hole recombination rates. The incorporation of phosphotungstic acid (PTA) in sample preparation effectively minimizes interference from compounds like L-cysteine and others. Consequently, this leads to an improvement in accuracy through the reduction of impurity levels. Appreciable photocurrent enhancements are observed upon introduction of both oxidized and reduced glutathione at the optimized composite photoanode. Coupled with advantageous features of photoelectrochemical transduction such as simplicity, cost-effectiveness, and resistance to fouling, this sensor holds great promise for practical applications in complex biological media.
Keyword ：
Glutathione Glutathione Photoelectrochemical sensor Photoelectrochemical sensor Phthalocyanine/TiO2 composite Phthalocyanine/TiO2 composite S-Scheme heterojunction S-Scheme heterojunction
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| GB/T 7714 | Zhang, Yu-Xuan , Wu, Wen-Ru , Zhao, Ning et al. S-scheme heterojunction phthalocyanine/TiO2 photoelectrochemical sensor for innovative glutathione detection [J]. | MICROCHIMICA ACTA , 2024 , 191 (7) . |
| MLA | Zhang, Yu-Xuan et al. "S-scheme heterojunction phthalocyanine/TiO2 photoelectrochemical sensor for innovative glutathione detection" . | MICROCHIMICA ACTA 191 . 7 (2024) . |
| APA | Zhang, Yu-Xuan , Wu, Wen-Ru , Zhao, Ning , Song, Yan-Song , Wang, Jian . S-scheme heterojunction phthalocyanine/TiO2 photoelectrochemical sensor for innovative glutathione detection . | MICROCHIMICA ACTA , 2024 , 191 (7) . |
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Homogeneous electrochemiluminescence (ECL) has gained attention for its simplicity and stability. However, false positives due to solution background interference pose a challenge. To address this, magnetic ECL nanoparticles (Fe3O4@Ru@SiO2 NPs) were synthesized, offering easy modification, magnetic separation, and stable luminescence. These were utilized in an ECL sensor for miRNA-155 (miR-155) detection, with locked DNAzyme and substrate chain (mDNA) modified on their surface. The poor conductivity of long-chain DNA significantly impacts the conductivity and electron transfer capability of Fe3O4@Ru@SiO2 NPs, resulting in weaker ECL signals. Upon target presence, unlocked DNAzyme catalyzes mDNA cleavage, leading to shortened DNA chains and reduced density. In contrast, the presence of short-chain DNA has minimal impact on the conductivity and electron transfer capability of Fe3O4@Ru@SiO2 NPs. Simultaneously, the material surface’s electronegativity decreases, weakening the electrostatic repulsion with the negatively charged electrode, resulting in the system detecting stronger ECL signals. This sensor enables homogeneous ECL detection while mitigating solution background interference through magnetic separation. Within a range of 100 fM to 10 nM, the sensor exhibits a linear relationship between ECL intensity and target concentration, with a 26.91 fM detection limit. It demonstrates high accuracy in clinical sample detection, holding significant potential for clinical diagnostics. Future integration with innovative detection strategies may further enhance sensitivity and specificity in biosensing applications. © 2024 American Chemical Society
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| GB/T 7714 | Lin, Y. , Luo, P. , Luo, F. et al. High-Sensitivity Homogeneous Detection of miRNA-155 Governed by DNA Walker-Regulated Surface DNA Density of Magnetic Electrochemiluminescence Nanoparticles [J]. | Analytical Chemistry , 2024 . |
| MLA | Lin, Y. et al. "High-Sensitivity Homogeneous Detection of miRNA-155 Governed by DNA Walker-Regulated Surface DNA Density of Magnetic Electrochemiluminescence Nanoparticles" . | Analytical Chemistry (2024) . |
| APA | Lin, Y. , Luo, P. , Luo, F. , Lin, C. , Wang, J. , Qiu, B. et al. High-Sensitivity Homogeneous Detection of miRNA-155 Governed by DNA Walker-Regulated Surface DNA Density of Magnetic Electrochemiluminescence Nanoparticles . | Analytical Chemistry , 2024 . |
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The detection of trypsin is significantly important for both clinical diagnosis and disease treatment. In this study, an innovative multicolor sensor for trypsin detection has been established based on the regulation of the peroxidase activity of bovine serum albumin-coated gold nanoclusters (BSA-Au NCs) and efficient etching of gold nanobipyramids (Au NBPs). BSA-Au NCs have slight peroxidase enzyme activity and can catalyze the oxidation of 3,3 ',5,5 '-tetramethylbenzidine (TMB) to generate TMB+, while trypsin can hydrolyze BSA ligands on the surface of BSA-Au NCs, thus exposing more catalytic active sites of BSA-Au NCs and resulting in the enhancement of the peroxidase activity of BSA-Au NCs, hence more TMB+ is generated. Under acidic conditions, TMB+ can etch Au NBPs efficiently, consequently affecting the aspect ratio of Au NBPs accompanied by the ultraviolet-visible (UV-vis) spectra blue shifting of the system. Furthermore, this also results in color variations that can be distinguished and recognized by naked eyes without any expensive and sophisticated instruments. This multicolor sensor has an available linear relationship with the logarithm of the trypsin concentration in the range of 0.1-100 mu g/mL, and the detection limit is 0.045 mu g/mL. The designed sensor has been used to detect the concentration of trypsin in human serum samples from healthy individuals and pancreatitis patients with satisfactory results.
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| GB/T 7714 | Luo, Qin , Tian, Mengjian , Luo, Fang et al. Multicolor Biosensor for Trypsin Detection Based on the Regulation of the Peroxidase Activity of Bovine Serum Albumin-Coated Gold Nanoclusters and Etching of Gold Nanobipyramids [J]. | ANALYTICAL CHEMISTRY , 2023 . |
| MLA | Luo, Qin et al. "Multicolor Biosensor for Trypsin Detection Based on the Regulation of the Peroxidase Activity of Bovine Serum Albumin-Coated Gold Nanoclusters and Etching of Gold Nanobipyramids" . | ANALYTICAL CHEMISTRY (2023) . |
| APA | Luo, Qin , Tian, Mengjian , Luo, Fang , Zhao, Min , Lin, Cuiying , Qiu, Bin et al. Multicolor Biosensor for Trypsin Detection Based on the Regulation of the Peroxidase Activity of Bovine Serum Albumin-Coated Gold Nanoclusters and Etching of Gold Nanobipyramids . | ANALYTICAL CHEMISTRY , 2023 . |
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A novel photoelectrochemical (PEC) sensor was constructed for the highly sensitive detection of reduced glutathione (GSH) based on the multiple catalytic properties of phosphotungstic acid (PTA). In this work, the catalytic properties of PTA were applied to PEC sensing for the first time and interpreted in detail. First, PTA as an electron acceptor can inhibit the complexation of photogenerated electron-hole pairs in p-Cu2O, thus significantly increasing the photogenerated current of p-type semiconductor material Cu2O. Secondly, when GSH is oxidized to oxidized glutathione (GSSG) by photogenerated holes on the photocathode, PTA is able to reduce GSSG to GSH by transferring protons, forming a redox cycle regeneration process of GSH. Finally, the relatively large amount of PTA in the background solution was able to pre-oxidize interfering substances such as l-cysteine and ascorbic acid, which improved the selectivity of the method. Under the optimal experimental conditions, the linear range of the PEC sensor response to GSH was 0.050-100 nmol L-1, with a detection limit as low as 0.017 nmol L-1 (S/N = 3), which can be applied to the detection of GSH content in cell lysate samples.
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| GB/T 7714 | Jiang, Yifan , Zhang, Huilan , Xu, Meizhu et al. Ultrasensitive photoelectrochemical detection of glutathione based on the multifunctional catalytic properties of phosphotungstic acid [J]. | ANALYST , 2023 , 148 (13) : 2983-2991 . |
| MLA | Jiang, Yifan et al. "Ultrasensitive photoelectrochemical detection of glutathione based on the multifunctional catalytic properties of phosphotungstic acid" . | ANALYST 148 . 13 (2023) : 2983-2991 . |
| APA | Jiang, Yifan , Zhang, Huilan , Xu, Meizhu , Luo, Fang , Lin, Cuiying , Qiu, Bin et al. Ultrasensitive photoelectrochemical detection of glutathione based on the multifunctional catalytic properties of phosphotungstic acid . | ANALYST , 2023 , 148 (13) , 2983-2991 . |
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In this work, an electrochemiluminescence (ECL) biosensor with dual signal enhancement was constructed and used for DNA adenine methylation methyltransferase (Dam MTase) detection. At present of Dam MTase, restriction endonuclease (DPnI) cleaves hairpin DNA (HP) and releases the HP stem end as a single strand that can activate CRISPR/Cas12a trans-cleavage activity. Assisted by trans-cleavage, the distance between the signal quenching factor ferrocene (Fc) and the ECL signal unit increased, and the repulsion between the signal unit and the Indium Tin Oxides (ITO) electrode decreased. The above results resulted in an enhanced ECL signal. ECL intensity has a good linear relationship with the logarithm of Dam MTase concentration in the range of 5-70 U/ mL with a detection limit of 23.4 mU/mL. The proposed biosensor was successfully utilized to detect of Dam MTase in serum samples.
Keyword ：
Biosensor Biosensor Cas Cas CRISPR CRISPR DNA adenine Methylation methyltransferase DNA adenine Methylation methyltransferase DNA methylation DNA methylation Electrochemiluminescence Electrochemiluminescence
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| GB/T 7714 | Lin, Cuiying , Huang, Qingqing , Tian, Mengjian et al. Electrochemiluminescence biosensor for DNA adenine methylation methyltransferase based on CRISPR/Cas12a trans-cleavage-induced dual signal enhancement [J]. | TALANTA , 2023 , 251 . |
| MLA | Lin, Cuiying et al. "Electrochemiluminescence biosensor for DNA adenine methylation methyltransferase based on CRISPR/Cas12a trans-cleavage-induced dual signal enhancement" . | TALANTA 251 (2023) . |
| APA | Lin, Cuiying , Huang, Qingqing , Tian, Mengjian , Luo, Fang , Wang, Jian , Qiu, Bin et al. Electrochemiluminescence biosensor for DNA adenine methylation methyltransferase based on CRISPR/Cas12a trans-cleavage-induced dual signal enhancement . | TALANTA , 2023 , 251 . |
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In this work, a novel photo-chemiluminescence (PCL) array imaging technique was developed to detect HIV DNA sequences using water-dispersed ultrathin sulfur-doped g-C3N4 porous nanosheets (SCNNSs) as photocatalysts, with complementary chains of HIV DNA as the biorecognition elements. The PCL response was enhanced when a suitable amount of SCNNSs was used. The large specific surface area and p-conjugated structure of the SCNNSs provided a good platform for immobilizing the complementary chains of HIV DNA. When DNA complementary chains were present, some of the catalytically active sites of SCNNSs were blocked, and the PCL of the platform was weakened. When the HIV DNA was added, the DNA double chain was far away from the surfaces of the SCNNSs because p-p the stacking interactions between the formed dsDNA and SCNNSs were weak. Therefore, the addition of the target HIV DNA sequence noticeably restored the signal. In the range of 5.00 x 10(-8) M to 200 x 10(-8) M, the enhanced PCL response was linearly related to the concentration of the HIV DNA sequence, and the detection limit (3S/N) was 1.50 x 10(-8) mol L-1. In addition, the combination of SCNNSs with complementary chains of HIV DNA successfully produced a high-performance PCL imaging sensor. In these proof-of-concept experiments, we demonstrated that our method was fast, portable, and ultra-sensitive, with high throughput.
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| GB/T 7714 | Zhang, Huilan , Jiang, Yifan , Wang, Jian et al. High-throughput photo-chemiluminescence imaging for HIV DNA determination based on a sulfur-doped graphitic carbonitride photocatalyst [J]. | ANALYTICAL METHODS , 2023 , 15 (17) : 2114-2120 . |
| MLA | Zhang, Huilan et al. "High-throughput photo-chemiluminescence imaging for HIV DNA determination based on a sulfur-doped graphitic carbonitride photocatalyst" . | ANALYTICAL METHODS 15 . 17 (2023) : 2114-2120 . |
| APA | Zhang, Huilan , Jiang, Yifan , Wang, Jian , Jiang, Zhou . High-throughput photo-chemiluminescence imaging for HIV DNA determination based on a sulfur-doped graphitic carbonitride photocatalyst . | ANALYTICAL METHODS , 2023 , 15 (17) , 2114-2120 . |
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The detection of trypsin is significantly important for both clinical diagnosis and disease treatment. In this study, an innovative multicolor sensor for trypsin detection has been established based on the regulation of the peroxidase activity of bovine serum albumin-coated gold nanoclusters (BSA-Au NCs) and have slight peroxidase enzyme activity and can catalyze the oxidation of 3,3 & PRIME;,5,5 & PRIME;-tetramethylbenzidine (TMB) to generate TMB+, while trypsin can hydrolyze BSA ligands on the surface of BSA-Au NCs, thus exposing more catalytic active sites of BSA-Au NCs and resulting in the enhancement of the peroxidase activity of BSA-Au NCs, hence more TMB+ is generated. Under acidic conditions, TMB+ can etch Au NBPs efficiently, consequently affecting the aspect ratio of Au NBPs accompanied by the ultraviolet-visible (UV-vis) spectra blue shifting of the system. Furthermore, this also results in color variations that can be distinguished and recognized by naked eyes without any expensive and sophisticated instruments. This multicolor sensor has an available linear relationship with the logarithm of the trypsin concentration in the range of 0.1-100 mu g/mL, and the detection limit is 0.045 mu g/mL. The designed sensor has been used to detect the concentration of trypsin in human serum samples from healthy individuals and pancreatitis patients with satisfactory results.
Cite：
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| GB/T 7714 | Luo, Qin , Tian, Mengjian , Luo, Fang et al. Multicolor Biosensor for Trypsin Detection Based on the Regulation of the Peroxidase Activity of Bovine Serum Albumin-Coated Gold Nanoclusters and Etching of Gold Nanobipyramids [J]. | ANALYTICAL CHEMISTRY , 2023 : 2390-2397 . |
| MLA | Luo, Qin et al. "Multicolor Biosensor for Trypsin Detection Based on the Regulation of the Peroxidase Activity of Bovine Serum Albumin-Coated Gold Nanoclusters and Etching of Gold Nanobipyramids" . | ANALYTICAL CHEMISTRY (2023) : 2390-2397 . |
| APA | Luo, Qin , Tian, Mengjian , Luo, Fang , Zhao, Min , Lin, Cuiying , Qiu, Bin et al. Multicolor Biosensor for Trypsin Detection Based on the Regulation of the Peroxidase Activity of Bovine Serum Albumin-Coated Gold Nanoclusters and Etching of Gold Nanobipyramids . | ANALYTICAL CHEMISTRY , 2023 , 2390-2397 . |
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Laser-induced fluorescence (LIF) is one of the most sensitive techniques for detection. As for the LIF with UV excitation, replacing UV laser with UV LED (i.e., UV LED-IF) has advantages of long life time and low cost. However, for LED-IF, the background signal (noise) would be much higher, due to the poor beam quality of LED. Here, an axial-excitation UV LED-IF with short metal capillary (MC) and black sealing (i.e., MC-LED-IF) was proposed for non-invasive detection of human saliva glucose (HSG). The SNR was improved 3.1-fold by employing the black sealing, due to the effective suppression of the background signal, which mainly results from light leakage. Moreover, the transmission loss of UV LED light in MC and saliva-filled MC was investigated, and an optimal MC length of only 4 cm was obtained. By using the MC-LED-IF, a detection limit of concentration (DLC) of 2.4 mu M was realized for detecting HSG via conventional 3-(4-hydroxyphenyl)-propionic acid (HPPA) reagent with good selectivity and reliability. The MC-LED-IF features low cost and compact (11 x 4 x 4 cm), which is suitable for personal use.
Keyword ：
Background signal Background signal capillary length capillary length LED-induced fluorescence LED-induced fluorescence metal capillary (MC) metal capillary (MC) saliva glucose detection saliva glucose detection
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| GB/T 7714 | Cai, Weicheng , Li, Zhaolin , Huang, Hui et al. A Compact UV-LED-Induced Fluorescence With Short Metal Capillary and Low Light Leakage: Application to Noninvasive Glucose Detection [J]. | IEEE SENSORS JOURNAL , 2023 , 23 (8) : 8366-8374 . |
| MLA | Cai, Weicheng et al. "A Compact UV-LED-Induced Fluorescence With Short Metal Capillary and Low Light Leakage: Application to Noninvasive Glucose Detection" . | IEEE SENSORS JOURNAL 23 . 8 (2023) : 8366-8374 . |
| APA | Cai, Weicheng , Li, Zhaolin , Huang, Hui , Li, Xuejing , Zhao, Jian , Liu, Pengbo et al. A Compact UV-LED-Induced Fluorescence With Short Metal Capillary and Low Light Leakage: Application to Noninvasive Glucose Detection . | IEEE SENSORS JOURNAL , 2023 , 23 (8) , 8366-8374 . |
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