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学者姓名：林翠英
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Abstract ：
Changes in the charge density on the inner surface of the microchannel can modulate the ion concentration at the tip, thus causing changes in the resistance of the system. In this study, this property is adopted to construct a portable sensor using a multimeter and aflatoxin B1 (AFB1) is used as the model target. Initially, the cDNA/aptamer complex is modified in the microchannel. The inner microchannel surface's charge density is then altered by the recognition of the target, leading to a change in the system's resistance, which can be conveniently monitored using a multimeter. Critical parameters influencing the performance of the system are optimized. Under optimum conditions, the resistance is linearly related to the logarithm of AFB1 concentration in the range of 100 fM-10 nM and the detection limit is 46 fM (S/N = 3). The resistive measurement is separated from the recognition reaction of the target, reducing the matrix interference during the detection process. This sensor boasts high sensitivity and specificity coupled with commendable reproducibility and stability. It is applied to assay the AFB1 content successfully in an actual sample of corn. Moreover, this approach is cost-effective, user-friendly, and highly accurate.
Keyword ：
aflatoxin B1 aflatoxin B1 microchannel microchannel portablesensor portablesensor resistance resistance surface charge density surface charge density
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| GB/T 7714 | Cai, Huabin , Huang, Yanling , Lin, Yue et al. Portable Sensor for Aflatoxin B1 Based on the Regulation of Resistance of a Microchannel Using a Multimeter as Readout [J]. | ACS SENSORS , 2024 , 9 (1) : 494-501 . |
| MLA | Cai, Huabin et al. "Portable Sensor for Aflatoxin B1 Based on the Regulation of Resistance of a Microchannel Using a Multimeter as Readout" . | ACS SENSORS 9 . 1 (2024) : 494-501 . |
| APA | Cai, Huabin , Huang, Yanling , Lin, Yue , Luo, Fang , Chen, Lifen , Guo, Longhua et al. Portable Sensor for Aflatoxin B1 Based on the Regulation of Resistance of a Microchannel Using a Multimeter as Readout . | ACS SENSORS , 2024 , 9 (1) , 494-501 . |
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The control of nanoparticle morphology can effectively change their properties. Adjusting the aggregation state of bovine serum albumin-coated gold nanoclusters (BSA-Au NCs) can regulate the enzyme-mimicking catalytic activity. Hyaluronic acid (HA) induces the aggregation of BSA-Au NCs, leading to the shielding of the catalytic active sites and a decrease in activity; this feature has been utilized to design a multicolor biosensor for hyaluronidase (HAase). The presence of HAase can hydrolyze the glycosidic bonds in HA, causing the aggregated BSA-Au NCs to disperse and express their catalytic activity, which in turn catalyzes the etching of Au nanobipyramids (Au-NBPs) in the presence of 3,3 ',5,5 ' -tetramethylbenzidine (TMB), resulting in a morphological transition from bipyramidal to ellipsoidal and spherical shapes, while the solution displays a variety of colors. Visual recognition of these multicolor changes establishes a relationship between the enzyme activity and color. Based on the controlled aggregation and dispersion of BSA-Au NCs and the etching of Au-NBPs, a simple multicolor HAase biosensor was designed. The proposed biosensor shows a linear response to HAase concentrations in the range of 5-80 U/mL, with clear color changes under optimized conditions. The limit of detection (LOD) of the sensor was determined to be 1.98 U/mL (LOD = 3s/k). This method successfully applies the changing of enzyme-mimicking catalytic activity nanoparticles for colorimetric analysis detection.
Keyword ：
aggregation statemodulation aggregation statemodulation Au nanocluster Au nanocluster hyaluronidase hyaluronidase multicolor multicolor nanozymes nanozymes
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| GB/T 7714 | Tian, Mengjian , Lin, Cuiying , Lin, Yue et al. Multicolor Biosensor of Hyaluronidase by the Regulation of Enzyme-Like Catalytic Activity of Au Nanoclusters through Changes in the Aggregation State [J]. | ACS APPLIED NANO MATERIALS , 2024 , 7 (5) : 5620-5627 . |
| MLA | Tian, Mengjian et al. "Multicolor Biosensor of Hyaluronidase by the Regulation of Enzyme-Like Catalytic Activity of Au Nanoclusters through Changes in the Aggregation State" . | ACS APPLIED NANO MATERIALS 7 . 5 (2024) : 5620-5627 . |
| APA | Tian, Mengjian , Lin, Cuiying , Lin, Yue , Luo, Fang , Qiu, Bin , Wang, Jian et al. Multicolor Biosensor of Hyaluronidase by the Regulation of Enzyme-Like Catalytic Activity of Au Nanoclusters through Changes in the Aggregation State . | ACS APPLIED NANO MATERIALS , 2024 , 7 (5) , 5620-5627 . |
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Halide perovskites have emerged as a highly promising class of photoelectric materials. However, the application of lead-based perovskites has been hindered by their toxicity and relatively weak stability. In this work, a composite material comprising a lead-free perovskite cesium copper iodide (CsCu2I3) nanocrystal and a metal-organic framework (MOF-801) has been synthesized through an in situ growth approach. The resulting composite material, denoted as CsCu2I3/MOF-801, demonstrates outstanding stability and exceptional optoelectronic characteristics. MOF-801 may serve a dual role by acting as a protective barrier between CsCu2I3 nanocrystals and the external environment, as well as promoting the efficient transfer of photogenerated charge carriers, thereby mitigating their recombination. Consequently, CsCu2I3/MOF-801 demonstrates its utility by providing both stability and a notably high initial photocurrent. Leveraging the inherent reactivity between H2S and the composite material, which results in the formation of Cu2S and structural alteration, an exceptionally sensitive photoelectrochemical sensor for H2S detection has been designed. This sensor exhibits a linear detection range spanning from 0.005 to 100 mu M with a remarkable detection limit of 1.67 nM, rendering it highly suitable for precise quantification of H2S in rat brains. This eco-friendly sensor significantly broadens the application horizon of perovskite materials and lays a robust foundation for their future commercialization.
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| GB/T 7714 | Mao, Mengfan , Zu, Yexin , Zhang, Yating et al. Photoelectrochemical Sensor for H2S Based on a Lead-Free Perovskite/Metal-Organic Framework Composite [J]. | ANALYTICAL CHEMISTRY , 2024 , 96 (10) : 4290-4298 . |
| MLA | Mao, Mengfan et al. "Photoelectrochemical Sensor for H2S Based on a Lead-Free Perovskite/Metal-Organic Framework Composite" . | ANALYTICAL CHEMISTRY 96 . 10 (2024) : 4290-4298 . |
| APA | Mao, Mengfan , Zu, Yexin , Zhang, Yating , Qiu, Yongzhen , Lin, Yue , Luo, Fang et al. Photoelectrochemical Sensor for H2S Based on a Lead-Free Perovskite/Metal-Organic Framework Composite . | ANALYTICAL CHEMISTRY , 2024 , 96 (10) , 4290-4298 . |
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The electrochemiluminescence (ECL) intensity can be regulated by ionic current passing through the microchannel, which broadened the regulation of the ECL sensors. But in the early reported sensors, the electrostatic repulsion and steric hindrance caused few targets to approach the interface of the microchannel driven by concentration difference, which reduced the detection efficiency and prolonged the detection period. In this study, different accumulation strategies, such as a positive electric field and different polarity electric fields, were designed to accumulate targets in the microchannel. The interaction of azide groups and hydrogen sulfide served as a research model. Hydrogen sulfide can react with the negatively charged azide groups in the microchannel surface to produce positively charged amino groups, decreasing the negative charge density of the microchannel and thus altering the ionic current and ECL intensity. The accumulation of hydrogen sulfide at the microchannel tip can increase the collision probability with azide groups to improve the detection efficiency, and the integration of accumulation and reaction can shorten the detection period to 28 min. The hydrogen sulfide concentration on the microchannel tip accumulated by applying different polarity electric fields was 22.3-fold higher than that accumulated by applying a positive electric field. The selected research model broadened the application range of a microchannel-based ECL sensor and confirmed the universality of the microchannel-based ECL sensor.
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| GB/T 7714 | Huang, Yanling , Cai, Huabin , Lin, Yue et al. Charge Density-Regulated Microchannel-Based Electrochemiluminescence Sensor for Hydrogen Sulfide Detection with a Highly Efficient Accumulation Strategy [J]. | ANALYTICAL CHEMISTRY , 2024 , 96 (13) : 5251-5257 . |
| MLA | Huang, Yanling et al. "Charge Density-Regulated Microchannel-Based Electrochemiluminescence Sensor for Hydrogen Sulfide Detection with a Highly Efficient Accumulation Strategy" . | ANALYTICAL CHEMISTRY 96 . 13 (2024) : 5251-5257 . |
| APA | Huang, Yanling , Cai, Huabin , Lin, Yue , Luo, Fang , Lin, Cuiying , Wang, Jian et al. Charge Density-Regulated Microchannel-Based Electrochemiluminescence Sensor for Hydrogen Sulfide Detection with a Highly Efficient Accumulation Strategy . | ANALYTICAL CHEMISTRY , 2024 , 96 (13) , 5251-5257 . |
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The entry of Hg2+ into the human body can lead to serious damage to some organs and it is vital to construct some simple but sensitive Hg2+ assay methods. A multicolor sensor for Hg2+ was constructed depended on the inhibition of peroxidase activity of Fe/Co-MIL-88(NH2) mediated by DNA and color change caused by the etching of gold nanobipyramids (Au NBPs). The enzymatic activity of Fe/Co-MIL-88(NH2) can be inhibited by adsorbing the T-rich oligonucleotide sequence on its surface. Hg2+ can combine with thymine of the T-rich oligonucleotide sequence to constitute T-Hg2+-T complex, and cause the transforming of T-rich oligonucleotide sequence into three-dimensional structure and desorbed from Fe/Co-MIL-88(NH2) surface. Which results in the restoration of the peroxidase activity of Fe/Co-MIL-88(NH2), and then it catalyzes the 3,3 & PRIME;,5,5 & PRIME;-tetramethylbenzidine (TMB) to engender TMB2+, TMB2+ can etch Au NBPs, this accompanied with a peak shift of the UV-vis spectrum and recognizable color changes of the system. Semi-quantitative can be realized using the multicolor changes of the Au NBPs as the reading mode. Furthermore, quantitative sensing can be achieved by measuring the UV-vis peak shift of the system. The linear response range is 1.0 nM-10 & mu;M, and the detection limit (LOD) is 0.28 nM.
Keyword ：
Fe/Co-MIL-88(NH 2 ) Fe/Co-MIL-88(NH 2 ) Gold nanobipyramids Gold nanobipyramids Multicolor Multicolor Nanozyme Nanozyme
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| GB/T 7714 | Luo, Qin , Chen, Yu , Chen, Lifen et al. Multicolor Hg2+sensor based on the regulation of peroxidase activity of Fe/ Co-MIL-88(NH2) and etching of gold nanobipyramids [J]. | MICROCHEMICAL JOURNAL , 2023 , 193 . |
| MLA | Luo, Qin et al. "Multicolor Hg2+sensor based on the regulation of peroxidase activity of Fe/ Co-MIL-88(NH2) and etching of gold nanobipyramids" . | MICROCHEMICAL JOURNAL 193 (2023) . |
| APA | Luo, Qin , Chen, Yu , Chen, Lifen , Chen, Zhonghui , Luo, Fang , Wang, Jian et al. Multicolor Hg2+sensor based on the regulation of peroxidase activity of Fe/ Co-MIL-88(NH2) and etching of gold nanobipyramids . | MICROCHEMICAL JOURNAL , 2023 , 193 . |
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T-2 toxin is widely found in nature and also contaminates field crops and grain storage, posing a potential threat to human health. In this study, an ultra-sensitive electrochemiluminescence (ECL) aptasensor has been developed for the detection of T-2 toxin using Ti3C2 MXene-based nanomaterials functionalized with gold nanorods (AuNRs) and Ru(bpy)32+ (Ti3C2@AuNRs-Ru) as the luminescent indicator. Specifically, gold nanorods are grown in situ on the surface of Ti3C2 MXene, and Ru(bpy)32+ is loaded onto Ti3C2@AuNRs via electrostatic force to form the Ti3C2@AuNRs-Ru luminescent probe, which is then modified on the electrode surface. The aptamer of T-2 toxin is connected to the electrode surface through Au-S bonds, and then the capture chain (cDNA) with a quenching probe of ferrocene is introduced to the system. Ferrocene can quench the luminescence of the probe, reducing the ECL signal. In the presence of T-2 toxin, it binds to the aptamer with higher affinity, so the quenching probe is competed down, resulting in the recovery of the ECL signal. The increase in ECL signal is linearly correlated with the logarithm of T-2 toxin concentration, with the detection range of 50 fg/mL to 50 ng/ mL and a detection limit of 6.44 fg/mL. This strategy utilizes the high catalytic activity and excellent conductivity of MXene, improving the sensitivity significantly. The proposed sensor has been successfully applied for the detection of actual samples and provides a new strategy for the detection of hazardous pollutants.
Keyword ：
Electrochemiluminescent aptasensor Electrochemiluminescent aptasensor Hazardous pollutant Hazardous pollutant Nanomaterials Nanomaterials T-2 toxin T-2 toxin
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| GB/T 7714 | Zhang, Junyi , Deng, Ye , Chen, Lifen et al. A novel electrochemiluminescence aptasensor using Ti3C2@AuNRs-Ru for ultra-sensitive detection of T-2 toxin [J]. | ELECTROCHIMICA ACTA , 2023 , 475 . |
| MLA | Zhang, Junyi et al. "A novel electrochemiluminescence aptasensor using Ti3C2@AuNRs-Ru for ultra-sensitive detection of T-2 toxin" . | ELECTROCHIMICA ACTA 475 (2023) . |
| APA | Zhang, Junyi , Deng, Ye , Chen, Lifen , Luo, Fang , Weng, Zuquan , Lin, Cuiying et al. A novel electrochemiluminescence aptasensor using Ti3C2@AuNRs-Ru for ultra-sensitive detection of T-2 toxin . | ELECTROCHIMICA ACTA , 2023 , 475 . |
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In this work, an electrochemiluminescence (ECL) biosensor with dual signal enhancement was constructed and used for DNA adenine methylation methyltransferase (Dam MTase) detection. At present of Dam MTase, restriction endonuclease (DPnI) cleaves hairpin DNA (HP) and releases the HP stem end as a single strand that can activate CRISPR/Cas12a trans-cleavage activity. Assisted by trans-cleavage, the distance between the signal quenching factor ferrocene (Fc) and the ECL signal unit increased, and the repulsion between the signal unit and the Indium Tin Oxides (ITO) electrode decreased. The above results resulted in an enhanced ECL signal. ECL intensity has a good linear relationship with the logarithm of Dam MTase concentration in the range of 5-70 U/ mL with a detection limit of 23.4 mU/mL. The proposed biosensor was successfully utilized to detect of Dam MTase in serum samples.
Keyword ：
Biosensor Biosensor Cas Cas CRISPR CRISPR DNA adenine Methylation methyltransferase DNA adenine Methylation methyltransferase DNA methylation DNA methylation Electrochemiluminescence Electrochemiluminescence
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| GB/T 7714 | Lin, Cuiying , Huang, Qingqing , Tian, Mengjian et al. Electrochemiluminescence biosensor for DNA adenine methylation methyltransferase based on CRISPR/Cas12a trans-cleavage-induced dual signal enhancement [J]. | TALANTA , 2023 , 251 . |
| MLA | Lin, Cuiying et al. "Electrochemiluminescence biosensor for DNA adenine methylation methyltransferase based on CRISPR/Cas12a trans-cleavage-induced dual signal enhancement" . | TALANTA 251 (2023) . |
| APA | Lin, Cuiying , Huang, Qingqing , Tian, Mengjian , Luo, Fang , Wang, Jian , Qiu, Bin et al. Electrochemiluminescence biosensor for DNA adenine methylation methyltransferase based on CRISPR/Cas12a trans-cleavage-induced dual signal enhancement . | TALANTA , 2023 , 251 . |
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The detection of trypsin is significantly important for both clinical diagnosis and disease treatment. In this study, an innovative multicolor sensor for trypsin detection has been established based on the regulation of the peroxidase activity of bovine serum albumin-coated gold nanoclusters (BSA-Au NCs) and efficient etching of gold nanobipyramids (Au NBPs). BSA-Au NCs have slight peroxidase enzyme activity and can catalyze the oxidation of 3,3 ',5,5 '-tetramethylbenzidine (TMB) to generate TMB+, while trypsin can hydrolyze BSA ligands on the surface of BSA-Au NCs, thus exposing more catalytic active sites of BSA-Au NCs and resulting in the enhancement of the peroxidase activity of BSA-Au NCs, hence more TMB+ is generated. Under acidic conditions, TMB+ can etch Au NBPs efficiently, consequently affecting the aspect ratio of Au NBPs accompanied by the ultraviolet-visible (UV-vis) spectra blue shifting of the system. Furthermore, this also results in color variations that can be distinguished and recognized by naked eyes without any expensive and sophisticated instruments. This multicolor sensor has an available linear relationship with the logarithm of the trypsin concentration in the range of 0.1-100 mu g/mL, and the detection limit is 0.045 mu g/mL. The designed sensor has been used to detect the concentration of trypsin in human serum samples from healthy individuals and pancreatitis patients with satisfactory results.
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| GB/T 7714 | Luo, Qin , Tian, Mengjian , Luo, Fang et al. Multicolor Biosensor for Trypsin Detection Based on the Regulation of the Peroxidase Activity of Bovine Serum Albumin-Coated Gold Nanoclusters and Etching of Gold Nanobipyramids [J]. | ANALYTICAL CHEMISTRY , 2023 . |
| MLA | Luo, Qin et al. "Multicolor Biosensor for Trypsin Detection Based on the Regulation of the Peroxidase Activity of Bovine Serum Albumin-Coated Gold Nanoclusters and Etching of Gold Nanobipyramids" . | ANALYTICAL CHEMISTRY (2023) . |
| APA | Luo, Qin , Tian, Mengjian , Luo, Fang , Zhao, Min , Lin, Cuiying , Qiu, Bin et al. Multicolor Biosensor for Trypsin Detection Based on the Regulation of the Peroxidase Activity of Bovine Serum Albumin-Coated Gold Nanoclusters and Etching of Gold Nanobipyramids . | ANALYTICAL CHEMISTRY , 2023 . |
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提出分析化学课程虚拟教研室建设的总体思路。依托“分析化学课程”虚拟教研室，广泛开展课程思政建设相关教学研究交流活动，建立“线上分析化学课程群”，跨校协作打造国家精品“分析化学”教学资源库，探索突破时空限制、“线上+线下”结合的教师教研模式、丰富教研活动，形成优质教学资源的共建共享，组织开展常态化“分析化学课程”虚拟教研室内教师培训。旨在打造中部、东部、西部、东北地区高校教师分析化学课程共建共享平台，构建“分析化学课程”高校教学学术共同体。
Keyword ：
分析化学 分析化学 教学研讨 教学研讨 虚拟教研室 虚拟教研室
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| GB/T 7714 | 王玉枝 , 宦双燕 , 张文清 et al. 分析化学课程虚拟教研室建设途径探索 [J]. | 大学化学 , 2023 , 38 (10) : 7-13 . |
| MLA | 王玉枝 et al. "分析化学课程虚拟教研室建设途径探索" . | 大学化学 38 . 10 (2023) : 7-13 . |
| APA | 王玉枝 , 宦双燕 , 张文清 , 曹秋娥 , 刘晨江 , 陈明丽 et al. 分析化学课程虚拟教研室建设途径探索 . | 大学化学 , 2023 , 38 (10) , 7-13 . |
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Two-dimensional (2D) layered MoS2 has good dispersion and adsorption properties, but being a narrow bandgap semiconductor limits its application in photoelectric sensing. In this study, a homogeneous photoelectrochemical sensor based on three-dimensional (3D) ZnO/Au/2D MoS2 is proposed for the ultrasensitive detection of tetracycline (TET). MoS2 is uniformly embedded on the 3D ZnO/Au surface by ordered self-assembly. The physical method of p-p interaction of MoS2 replaces the conventional use of chemically modifying aptamers on the electrode material surface. Under optimal conditions, this method has been successfully applied to the detection of TET in milk, honey, pig kidney and pork samples with reliable results. We believe that this study presents a method for the preparation of sensing carriers and target detection with great potential for application.
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| GB/T 7714 | Li, Weixin , Wang, Xinyang , Chen, Lifen et al. A photoelectrochemical aptasensor for tetracycline based on the self-assembly of 2D MoS2 on a 3D ZnO/Au/ITO electrode [J]. | ANALYST , 2023 , 148 (20) : 4995-5001 . |
| MLA | Li, Weixin et al. "A photoelectrochemical aptasensor for tetracycline based on the self-assembly of 2D MoS2 on a 3D ZnO/Au/ITO electrode" . | ANALYST 148 . 20 (2023) : 4995-5001 . |
| APA | Li, Weixin , Wang, Xinyang , Chen, Lifen , Luo, Fang , Guo, Longhua , Lin, Cuiying et al. A photoelectrochemical aptasensor for tetracycline based on the self-assembly of 2D MoS2 on a 3D ZnO/Au/ITO electrode . | ANALYST , 2023 , 148 (20) , 4995-5001 . |
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