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学者姓名：林翠英
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Abstract ：
Halide perovskites have emerged as a highly promising class of photoelectric materials. However, the application of lead-based perovskites has been hindered by their toxicity and relatively weak stability. In this work, a composite material comprising a lead-free perovskite cesium copper iodide (CsCu2I3) nanocrystal and a metal-organic framework (MOF-801) has been synthesized through an in situ growth approach. The resulting composite material, denoted as CsCu2I3/MOF-801, demonstrates outstanding stability and exceptional optoelectronic characteristics. MOF-801 may serve a dual role by acting as a protective barrier between CsCu2I3 nanocrystals and the external environment, as well as promoting the efficient transfer of photogenerated charge carriers, thereby mitigating their recombination. Consequently, CsCu2I3/MOF-801 demonstrates its utility by providing both stability and a notably high initial photocurrent. Leveraging the inherent reactivity between H2S and the composite material, which results in the formation of Cu2S and structural alteration, an exceptionally sensitive photoelectrochemical sensor for H2S detection has been designed. This sensor exhibits a linear detection range spanning from 0.005 to 100 mu M with a remarkable detection limit of 1.67 nM, rendering it highly suitable for precise quantification of H2S in rat brains. This eco-friendly sensor significantly broadens the application horizon of perovskite materials and lays a robust foundation for their future commercialization.
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| GB/T 7714 | Mao, Mengfan , Zu, Yexin , Zhang, Yating et al. Photoelectrochemical Sensor for H2S Based on a Lead-Free Perovskite/Metal-Organic Framework Composite [J]. | ANALYTICAL CHEMISTRY , 2024 , 96 (10) : 4290-4298 . |
| MLA | Mao, Mengfan et al. "Photoelectrochemical Sensor for H2S Based on a Lead-Free Perovskite/Metal-Organic Framework Composite" . | ANALYTICAL CHEMISTRY 96 . 10 (2024) : 4290-4298 . |
| APA | Mao, Mengfan , Zu, Yexin , Zhang, Yating , Qiu, Yongzhen , Lin, Yue , Luo, Fang et al. Photoelectrochemical Sensor for H2S Based on a Lead-Free Perovskite/Metal-Organic Framework Composite . | ANALYTICAL CHEMISTRY , 2024 , 96 (10) , 4290-4298 . |
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Changes in the charge density on the inner surface of the microchannel can modulate the ion concentration at the tip, thus causing changes in the resistance of the system. In this study, this property is adopted to construct a portable sensor using a multimeter and aflatoxin B1 (AFB1) is used as the model target. Initially, the cDNA/aptamer complex is modified in the microchannel. The inner microchannel surface's charge density is then altered by the recognition of the target, leading to a change in the system's resistance, which can be conveniently monitored using a multimeter. Critical parameters influencing the performance of the system are optimized. Under optimum conditions, the resistance is linearly related to the logarithm of AFB1 concentration in the range of 100 fM-10 nM and the detection limit is 46 fM (S/N = 3). The resistive measurement is separated from the recognition reaction of the target, reducing the matrix interference during the detection process. This sensor boasts high sensitivity and specificity coupled with commendable reproducibility and stability. It is applied to assay the AFB1 content successfully in an actual sample of corn. Moreover, this approach is cost-effective, user-friendly, and highly accurate.
Keyword ：
aflatoxin B1 aflatoxin B1 microchannel microchannel portablesensor portablesensor resistance resistance surface charge density surface charge density
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| GB/T 7714 | Cai, Huabin , Huang, Yanling , Lin, Yue et al. Portable Sensor for Aflatoxin B1 Based on the Regulation of Resistance of a Microchannel Using a Multimeter as Readout [J]. | ACS SENSORS , 2024 , 9 (1) : 494-501 . |
| MLA | Cai, Huabin et al. "Portable Sensor for Aflatoxin B1 Based on the Regulation of Resistance of a Microchannel Using a Multimeter as Readout" . | ACS SENSORS 9 . 1 (2024) : 494-501 . |
| APA | Cai, Huabin , Huang, Yanling , Lin, Yue , Luo, Fang , Chen, Lifen , Guo, Longhua et al. Portable Sensor for Aflatoxin B1 Based on the Regulation of Resistance of a Microchannel Using a Multimeter as Readout . | ACS SENSORS , 2024 , 9 (1) , 494-501 . |
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The control of nanoparticle morphology can effectively change their properties. Adjusting the aggregation state of bovine serum albumin-coated gold nanoclusters (BSA-Au NCs) can regulate the enzyme-mimicking catalytic activity. Hyaluronic acid (HA) induces the aggregation of BSA-Au NCs, leading to the shielding of the catalytic active sites and a decrease in activity; this feature has been utilized to design a multicolor biosensor for hyaluronidase (HAase). The presence of HAase can hydrolyze the glycosidic bonds in HA, causing the aggregated BSA-Au NCs to disperse and express their catalytic activity, which in turn catalyzes the etching of Au nanobipyramids (Au-NBPs) in the presence of 3,3 ',5,5 ' -tetramethylbenzidine (TMB), resulting in a morphological transition from bipyramidal to ellipsoidal and spherical shapes, while the solution displays a variety of colors. Visual recognition of these multicolor changes establishes a relationship between the enzyme activity and color. Based on the controlled aggregation and dispersion of BSA-Au NCs and the etching of Au-NBPs, a simple multicolor HAase biosensor was designed. The proposed biosensor shows a linear response to HAase concentrations in the range of 5-80 U/mL, with clear color changes under optimized conditions. The limit of detection (LOD) of the sensor was determined to be 1.98 U/mL (LOD = 3s/k). This method successfully applies the changing of enzyme-mimicking catalytic activity nanoparticles for colorimetric analysis detection.
Keyword ：
aggregation statemodulation aggregation statemodulation Au nanocluster Au nanocluster hyaluronidase hyaluronidase multicolor multicolor nanozymes nanozymes
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| GB/T 7714 | Tian, Mengjian , Lin, Cuiying , Lin, Yue et al. Multicolor Biosensor of Hyaluronidase by the Regulation of Enzyme-Like Catalytic Activity of Au Nanoclusters through Changes in the Aggregation State [J]. | ACS APPLIED NANO MATERIALS , 2024 , 7 (5) : 5620-5627 . |
| MLA | Tian, Mengjian et al. "Multicolor Biosensor of Hyaluronidase by the Regulation of Enzyme-Like Catalytic Activity of Au Nanoclusters through Changes in the Aggregation State" . | ACS APPLIED NANO MATERIALS 7 . 5 (2024) : 5620-5627 . |
| APA | Tian, Mengjian , Lin, Cuiying , Lin, Yue , Luo, Fang , Qiu, Bin , Wang, Jian et al. Multicolor Biosensor of Hyaluronidase by the Regulation of Enzyme-Like Catalytic Activity of Au Nanoclusters through Changes in the Aggregation State . | ACS APPLIED NANO MATERIALS , 2024 , 7 (5) , 5620-5627 . |
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The electrochemiluminescence (ECL) intensity can be regulated by ionic current passing through the microchannel, which broadened the regulation of the ECL sensors. But in the early reported sensors, the electrostatic repulsion and steric hindrance caused few targets to approach the interface of the microchannel driven by concentration difference, which reduced the detection efficiency and prolonged the detection period. In this study, different accumulation strategies, such as a positive electric field and different polarity electric fields, were designed to accumulate targets in the microchannel. The interaction of azide groups and hydrogen sulfide served as a research model. Hydrogen sulfide can react with the negatively charged azide groups in the microchannel surface to produce positively charged amino groups, decreasing the negative charge density of the microchannel and thus altering the ionic current and ECL intensity. The accumulation of hydrogen sulfide at the microchannel tip can increase the collision probability with azide groups to improve the detection efficiency, and the integration of accumulation and reaction can shorten the detection period to 28 min. The hydrogen sulfide concentration on the microchannel tip accumulated by applying different polarity electric fields was 22.3-fold higher than that accumulated by applying a positive electric field. The selected research model broadened the application range of a microchannel-based ECL sensor and confirmed the universality of the microchannel-based ECL sensor.
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| GB/T 7714 | Huang, Yanling , Cai, Huabin , Lin, Yue et al. Charge Density-Regulated Microchannel-Based Electrochemiluminescence Sensor for Hydrogen Sulfide Detection with a Highly Efficient Accumulation Strategy [J]. | ANALYTICAL CHEMISTRY , 2024 , 96 (13) : 5251-5257 . |
| MLA | Huang, Yanling et al. "Charge Density-Regulated Microchannel-Based Electrochemiluminescence Sensor for Hydrogen Sulfide Detection with a Highly Efficient Accumulation Strategy" . | ANALYTICAL CHEMISTRY 96 . 13 (2024) : 5251-5257 . |
| APA | Huang, Yanling , Cai, Huabin , Lin, Yue , Luo, Fang , Lin, Cuiying , Wang, Jian et al. Charge Density-Regulated Microchannel-Based Electrochemiluminescence Sensor for Hydrogen Sulfide Detection with a Highly Efficient Accumulation Strategy . | ANALYTICAL CHEMISTRY , 2024 , 96 (13) , 5251-5257 . |
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Homogeneous electrochemiluminescence (ECL) has gained attention for its simplicity and stability. However, false positives due to solution background interference pose a challenge. To address this, magnetic ECL nanoparticles (Fe3O4@Ru@SiO2 NPs) were synthesized, offering easy modification, magnetic separation, and stable luminescence. These were utilized in an ECL sensor for miRNA-155 (miR-155) detection, with locked DNAzyme and substrate chain (mDNA) modified on their surface. The poor conductivity of long-chain DNA significantly impacts the conductivity and electron transfer capability of Fe3O4@Ru@SiO2 NPs, resulting in weaker ECL signals. Upon target presence, unlocked DNAzyme catalyzes mDNA cleavage, leading to shortened DNA chains and reduced density. In contrast, the presence of short-chain DNA has minimal impact on the conductivity and electron transfer capability of Fe3O4@Ru@SiO2 NPs. Simultaneously, the material surface’s electronegativity decreases, weakening the electrostatic repulsion with the negatively charged electrode, resulting in the system detecting stronger ECL signals. This sensor enables homogeneous ECL detection while mitigating solution background interference through magnetic separation. Within a range of 100 fM to 10 nM, the sensor exhibits a linear relationship between ECL intensity and target concentration, with a 26.91 fM detection limit. It demonstrates high accuracy in clinical sample detection, holding significant potential for clinical diagnostics. Future integration with innovative detection strategies may further enhance sensitivity and specificity in biosensing applications. © 2024 American Chemical Society
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| GB/T 7714 | Lin, Y. , Luo, P. , Luo, F. et al. High-Sensitivity Homogeneous Detection of miRNA-155 Governed by DNA Walker-Regulated Surface DNA Density of Magnetic Electrochemiluminescence Nanoparticles [J]. | Analytical Chemistry , 2024 . |
| MLA | Lin, Y. et al. "High-Sensitivity Homogeneous Detection of miRNA-155 Governed by DNA Walker-Regulated Surface DNA Density of Magnetic Electrochemiluminescence Nanoparticles" . | Analytical Chemistry (2024) . |
| APA | Lin, Y. , Luo, P. , Luo, F. , Lin, C. , Wang, J. , Qiu, B. et al. High-Sensitivity Homogeneous Detection of miRNA-155 Governed by DNA Walker-Regulated Surface DNA Density of Magnetic Electrochemiluminescence Nanoparticles . | Analytical Chemistry , 2024 . |
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The detection of trypsin is significantly important for both clinical diagnosis and disease treatment. In this study, an innovative multicolor sensor for trypsin detection has been established based on the regulation of the peroxidase activity of bovine serum albumin-coated gold nanoclusters (BSA-Au NCs) and efficient etching of gold nanobipyramids (Au NBPs). BSA-Au NCs have slight peroxidase enzyme activity and can catalyze the oxidation of 3,3 ',5,5 '-tetramethylbenzidine (TMB) to generate TMB+, while trypsin can hydrolyze BSA ligands on the surface of BSA-Au NCs, thus exposing more catalytic active sites of BSA-Au NCs and resulting in the enhancement of the peroxidase activity of BSA-Au NCs, hence more TMB+ is generated. Under acidic conditions, TMB+ can etch Au NBPs efficiently, consequently affecting the aspect ratio of Au NBPs accompanied by the ultraviolet-visible (UV-vis) spectra blue shifting of the system. Furthermore, this also results in color variations that can be distinguished and recognized by naked eyes without any expensive and sophisticated instruments. This multicolor sensor has an available linear relationship with the logarithm of the trypsin concentration in the range of 0.1-100 mu g/mL, and the detection limit is 0.045 mu g/mL. The designed sensor has been used to detect the concentration of trypsin in human serum samples from healthy individuals and pancreatitis patients with satisfactory results.
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| GB/T 7714 | Luo, Qin , Tian, Mengjian , Luo, Fang et al. Multicolor Biosensor for Trypsin Detection Based on the Regulation of the Peroxidase Activity of Bovine Serum Albumin-Coated Gold Nanoclusters and Etching of Gold Nanobipyramids [J]. | ANALYTICAL CHEMISTRY , 2023 . |
| MLA | Luo, Qin et al. "Multicolor Biosensor for Trypsin Detection Based on the Regulation of the Peroxidase Activity of Bovine Serum Albumin-Coated Gold Nanoclusters and Etching of Gold Nanobipyramids" . | ANALYTICAL CHEMISTRY (2023) . |
| APA | Luo, Qin , Tian, Mengjian , Luo, Fang , Zhao, Min , Lin, Cuiying , Qiu, Bin et al. Multicolor Biosensor for Trypsin Detection Based on the Regulation of the Peroxidase Activity of Bovine Serum Albumin-Coated Gold Nanoclusters and Etching of Gold Nanobipyramids . | ANALYTICAL CHEMISTRY , 2023 . |
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Point-of-care testing (POCT) has experienced rapid development owing to its advantages of rapid testing, low cost and strong operability, making it indispensable for analyte detection in outdoor or rural areas. In this study, we propose a novel method for the detection of aflatoxin B1 (AFB1) using a dual-signal readout approach within a unified system. This method employs dual channel modes, namely visual fluorescence and weight measurements, as the signal readouts. Specifically, a pressure-sensitive material is utilized as a visual fluorescent agent, its signal can be quenched in the presence of high oxygen pressure. Additionally, an electronic balance, commonly used for weight measurement, is adopted as another signal device, where the signal is generated through the catalytic decomposition of H2O2 by platinum nanoparticles. The experimental results demonstrate that the proposed device enables accurate AFB1 detection within the concentration range of 1.5-32 & mu;g mL-1, with a detection limit of 0.47 & mu;g mL-1. Moreover, this method has been successfully applied for practical AFB1 detection with satisfactory results. Notably, this study pioneers the use of a pressure-sensitive material as a visual signal in POCT. By addressing the limitations of single-signal readout approaches, our method fulfills requirements of intuitiveness, sensitivity, quantitative analysis and reusability.
Keyword ：
Dual-mode readout Dual-mode readout Fluorescence Fluorescence Platinum nanoparticles Platinum nanoparticles Point-of-care testing Point-of-care testing
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| GB/T 7714 | Luo, Fang , Zhan, Linxiu , Deng, Ye et al. Oxygen-induced dual-signal point-of-care testing aptasensor for aflatoxin B1 detection using platinum nanoparticle catalysis in visual fluorometry and gravimetry [J]. | ANALYTICA CHIMICA ACTA , 2023 , 1273 . |
| MLA | Luo, Fang et al. "Oxygen-induced dual-signal point-of-care testing aptasensor for aflatoxin B1 detection using platinum nanoparticle catalysis in visual fluorometry and gravimetry" . | ANALYTICA CHIMICA ACTA 1273 (2023) . |
| APA | Luo, Fang , Zhan, Linxiu , Deng, Ye , Qiao, Kun , Pan, Nan , Weng, Zuquan et al. Oxygen-induced dual-signal point-of-care testing aptasensor for aflatoxin B1 detection using platinum nanoparticle catalysis in visual fluorometry and gravimetry . | ANALYTICA CHIMICA ACTA , 2023 , 1273 . |
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A novel photoelectrochemical (PEC) sensor was constructed for the highly sensitive detection of reduced glutathione (GSH) based on the multiple catalytic properties of phosphotungstic acid (PTA). In this work, the catalytic properties of PTA were applied to PEC sensing for the first time and interpreted in detail. First, PTA as an electron acceptor can inhibit the complexation of photogenerated electron-hole pairs in p-Cu2O, thus significantly increasing the photogenerated current of p-type semiconductor material Cu2O. Secondly, when GSH is oxidized to oxidized glutathione (GSSG) by photogenerated holes on the photocathode, PTA is able to reduce GSSG to GSH by transferring protons, forming a redox cycle regeneration process of GSH. Finally, the relatively large amount of PTA in the background solution was able to pre-oxidize interfering substances such as l-cysteine and ascorbic acid, which improved the selectivity of the method. Under the optimal experimental conditions, the linear range of the PEC sensor response to GSH was 0.050-100 nmol L-1, with a detection limit as low as 0.017 nmol L-1 (S/N = 3), which can be applied to the detection of GSH content in cell lysate samples.
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| GB/T 7714 | Jiang, Yifan , Zhang, Huilan , Xu, Meizhu et al. Ultrasensitive photoelectrochemical detection of glutathione based on the multifunctional catalytic properties of phosphotungstic acid [J]. | ANALYST , 2023 , 148 (13) : 2983-2991 . |
| MLA | Jiang, Yifan et al. "Ultrasensitive photoelectrochemical detection of glutathione based on the multifunctional catalytic properties of phosphotungstic acid" . | ANALYST 148 . 13 (2023) : 2983-2991 . |
| APA | Jiang, Yifan , Zhang, Huilan , Xu, Meizhu , Luo, Fang , Lin, Cuiying , Qiu, Bin et al. Ultrasensitive photoelectrochemical detection of glutathione based on the multifunctional catalytic properties of phosphotungstic acid . | ANALYST , 2023 , 148 (13) , 2983-2991 . |
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In this work, an electrochemiluminescence (ECL) biosensor with dual signal enhancement was constructed and used for DNA adenine methylation methyltransferase (Dam MTase) detection. At present of Dam MTase, restriction endonuclease (DPnI) cleaves hairpin DNA (HP) and releases the HP stem end as a single strand that can activate CRISPR/Cas12a trans-cleavage activity. Assisted by trans-cleavage, the distance between the signal quenching factor ferrocene (Fc) and the ECL signal unit increased, and the repulsion between the signal unit and the Indium Tin Oxides (ITO) electrode decreased. The above results resulted in an enhanced ECL signal. ECL intensity has a good linear relationship with the logarithm of Dam MTase concentration in the range of 5-70 U/ mL with a detection limit of 23.4 mU/mL. The proposed biosensor was successfully utilized to detect of Dam MTase in serum samples.
Keyword ：
Biosensor Biosensor Cas Cas CRISPR CRISPR DNA adenine Methylation methyltransferase DNA adenine Methylation methyltransferase DNA methylation DNA methylation Electrochemiluminescence Electrochemiluminescence
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| GB/T 7714 | Lin, Cuiying , Huang, Qingqing , Tian, Mengjian et al. Electrochemiluminescence biosensor for DNA adenine methylation methyltransferase based on CRISPR/Cas12a trans-cleavage-induced dual signal enhancement [J]. | TALANTA , 2023 , 251 . |
| MLA | Lin, Cuiying et al. "Electrochemiluminescence biosensor for DNA adenine methylation methyltransferase based on CRISPR/Cas12a trans-cleavage-induced dual signal enhancement" . | TALANTA 251 (2023) . |
| APA | Lin, Cuiying , Huang, Qingqing , Tian, Mengjian , Luo, Fang , Wang, Jian , Qiu, Bin et al. Electrochemiluminescence biosensor for DNA adenine methylation methyltransferase based on CRISPR/Cas12a trans-cleavage-induced dual signal enhancement . | TALANTA , 2023 , 251 . |
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The detection of trypsin is significantly important for both clinical diagnosis and disease treatment. In this study, an innovative multicolor sensor for trypsin detection has been established based on the regulation of the peroxidase activity of bovine serum albumin-coated gold nanoclusters (BSA-Au NCs) and have slight peroxidase enzyme activity and can catalyze the oxidation of 3,3 & PRIME;,5,5 & PRIME;-tetramethylbenzidine (TMB) to generate TMB+, while trypsin can hydrolyze BSA ligands on the surface of BSA-Au NCs, thus exposing more catalytic active sites of BSA-Au NCs and resulting in the enhancement of the peroxidase activity of BSA-Au NCs, hence more TMB+ is generated. Under acidic conditions, TMB+ can etch Au NBPs efficiently, consequently affecting the aspect ratio of Au NBPs accompanied by the ultraviolet-visible (UV-vis) spectra blue shifting of the system. Furthermore, this also results in color variations that can be distinguished and recognized by naked eyes without any expensive and sophisticated instruments. This multicolor sensor has an available linear relationship with the logarithm of the trypsin concentration in the range of 0.1-100 mu g/mL, and the detection limit is 0.045 mu g/mL. The designed sensor has been used to detect the concentration of trypsin in human serum samples from healthy individuals and pancreatitis patients with satisfactory results.
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| GB/T 7714 | Luo, Qin , Tian, Mengjian , Luo, Fang et al. Multicolor Biosensor for Trypsin Detection Based on the Regulation of the Peroxidase Activity of Bovine Serum Albumin-Coated Gold Nanoclusters and Etching of Gold Nanobipyramids [J]. | ANALYTICAL CHEMISTRY , 2023 : 2390-2397 . |
| MLA | Luo, Qin et al. "Multicolor Biosensor for Trypsin Detection Based on the Regulation of the Peroxidase Activity of Bovine Serum Albumin-Coated Gold Nanoclusters and Etching of Gold Nanobipyramids" . | ANALYTICAL CHEMISTRY (2023) : 2390-2397 . |
| APA | Luo, Qin , Tian, Mengjian , Luo, Fang , Zhao, Min , Lin, Cuiying , Qiu, Bin et al. Multicolor Biosensor for Trypsin Detection Based on the Regulation of the Peroxidase Activity of Bovine Serum Albumin-Coated Gold Nanoclusters and Etching of Gold Nanobipyramids . | ANALYTICAL CHEMISTRY , 2023 , 2390-2397 . |
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