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学者姓名：郭良洽
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Abstract ：
Typical fluorescent biosensors use fluorescently labeled ssDNA for target recognition and nanomaterials for signal transduction. Herein, we propose a reverse sensing strategy that Mo5N6 nanosheets are used for target recognition while fluorescein (FAM)-labeled ssDNA only serves for signal generation. We discover that Mo5N6 nanosheets show high fluorescence quenching ability (>95%) and selective recognition for sodium hexametaphosphate (SHMP). After FAM-labeled ssDNA is adsorbed on Mo5N6 nanosheets, the fluorescence is quenched due to the photoinduced electron transfer (PET) effect between FAM and Mo5N6 nanosheets. SHMP can specifically displace the adsorbed FAM-labeled ssDNA from Mo5N6 nanosheets, resulting in more than 80% fluorescence recovery on addition of 5 mu mol L-1 SHMP. This biosensor can sensitively detect SHMP down to 150 nmol L-1 and selectively recognize SHMP over glucose, lactose, common amino acids, Zn2+, Mg2+, Ca2+ and other phosphates (such as Na2HPO4, sodium pyrophosphate, sodium tripolyphosphate). This biosensor also shows great potential for the detection of SHMP in bacon sample. This work not only provides a facile sensitive and selective biosensor for SHMP but also exploits the application of transition metal nitrides in the field of sensing and biosensing.
Keyword ：
Biosensor Biosensor Food safety Food safety Molybdenum nitrides Molybdenum nitrides Sodium hexametaphosphate Sodium hexametaphosphate Turn-on Turn-on
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| GB/T 7714 | Huang, Wenying , Wu, Yali , Xia, Chunqiu et al. Mo5N6 nanosheets for fluorescent quenching and target recognition: Highly selectively sensing of sodium hexametaphosphate [J]. | TALANTA , 2024 , 273 . |
| MLA | Huang, Wenying et al. "Mo5N6 nanosheets for fluorescent quenching and target recognition: Highly selectively sensing of sodium hexametaphosphate" . | TALANTA 273 (2024) . |
| APA | Huang, Wenying , Wu, Yali , Xia, Chunqiu , Guo, Liangqia . Mo5N6 nanosheets for fluorescent quenching and target recognition: Highly selectively sensing of sodium hexametaphosphate . | TALANTA , 2024 , 273 . |
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It is of vital significance to explore effective and economical multifunctional materials for the detection, remediation and recycling of heavy metal (Cr(VI)). Herein, two-dimensional (2D) phenyl -modified carbon nitride (PCN) polymer was prepared through thermal polymerization of phenylguanidine carbonate. The incorporation of phenyl groups endowed PCN polymer with both strong green fluorescence and high photocatalytic activity for the reduction of Cr(VI). An multifunctional gel for simultaneous detection, adsorption, photo -reduction and recycling of Cr(VI) was further prepared by assembling PCN with polyethyleneimine-N,N'-methyl- enebisacrylamide (PEI -MBA) gel. PCN-PEI-MBA gel showed a sensitive fluorescence / colorimetric dual -mode detection of Cr(VI). Meanwhile, PCN-PEI-MBA gel displayed preeminent adsorption capacity of 3055.9 mg g-1 MBA gel was photocatalytically reduced into Cr(III) with visual real-time monitoring the detoxification process and Cr(III) can be further recycled as Cr2O3 by calcination of PCN-PEI-MBA gel. Finally, PCN-PEI-MBA gel was utilize to adsorb and recycle Cr(VI) from the chromium -plating wastewater and detect the concentration of remaining Cr(VI) in the wastewater. This work provides an all -in -one treatment strategy for the detection, remediation and resource recycling of Cr(VI) in practical wastewater. , surpassing previously reported adsorbents. Under visible -light irradiation, the absorbed Cr(VI) in PCN-PEI
Keyword ：
Adsorption Adsorption Carbon nitride Carbon nitride Chromium ion Chromium ion Chromium recycling Chromium recycling Detection Detection Photocatalytic reduction Photocatalytic reduction
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| GB/T 7714 | Chen, Jingru , Qiu, Yonghan , Liu, Xiaotao et al. Phenyl-modified carbon nitride based multifunctional gel for the detection, adsorption, photocatalytic reduction and recycling of chromium(VI) in wastewater [J]. | APPLIED SURFACE SCIENCE , 2024 , 652 . |
| MLA | Chen, Jingru et al. "Phenyl-modified carbon nitride based multifunctional gel for the detection, adsorption, photocatalytic reduction and recycling of chromium(VI) in wastewater" . | APPLIED SURFACE SCIENCE 652 (2024) . |
| APA | Chen, Jingru , Qiu, Yonghan , Liu, Xiaotao , Guo, Liangqia . Phenyl-modified carbon nitride based multifunctional gel for the detection, adsorption, photocatalytic reduction and recycling of chromium(VI) in wastewater . | APPLIED SURFACE SCIENCE , 2024 , 652 . |
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Fluorescent labeling and imaging is a useful tool to track the temporal and spatial distribution of microplastics (MPs) with size and shape features. However, the limited fluorescently labeled MPs spheres, photo -bleaching and leaching of organic small molecular dyes hamper the tracking of MPs in the environment. In this work, phenyl -modified carbon nitride (PMCN) fluorescent polymer was prepared and applied for the staining of eight different types of MPs. The interface interaction mechanism between PMCN fluorescent polymer and MPs was investigated by the desorption experiments. The long-time photo -stability of the stained MPs against ultraviolet (UV) light irradiation, freeze -thaw cycles, various environmental factors and real environmental water samples was evaluated. The MPs stained with PMCN fluorescent polymer showed more robust stability than the common dye (Nile red) against the damage when exposure to UV light for 72 h, treatment with freeze -thaw cycles for 60 days, exposure to strong acid, strong alkali, high salinity, freshwater (river and lake) or seawater for 60 days. Results indicate PMCN fluorescent polymer is promising as a convenient, efficient and universal fluorescent tracer for visually tracking the migration and distribution of MPs in environmental water system.
Keyword ：
Environmental water Environmental water Fluorescence image Fluorescence image Microplastics Microplastics Migration Migration Tracking Tracking
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| GB/T 7714 | Feng, Qiaocheng , Cui, Mei , Chen, Jingru et al. Enhanced long-term stability of stained microplastics with carbon nitride fluorescent polymer for tracking [J]. | ENVIRONMENTAL TECHNOLOGY & INNOVATION , 2024 , 34 . |
| MLA | Feng, Qiaocheng et al. "Enhanced long-term stability of stained microplastics with carbon nitride fluorescent polymer for tracking" . | ENVIRONMENTAL TECHNOLOGY & INNOVATION 34 (2024) . |
| APA | Feng, Qiaocheng , Cui, Mei , Chen, Jingru , Zheng, Xueyi , Guo, Liangqia . Enhanced long-term stability of stained microplastics with carbon nitride fluorescent polymer for tracking . | ENVIRONMENTAL TECHNOLOGY & INNOVATION , 2024 , 34 . |
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MicroRNAs (miRNAs) are pivotal in regulating biological processes such as cell proliferation and disease progression. Traditional miRNA detection methods like qRT-PCR and Northern blotting do not allow for monitoring dynamic changes in living cells and typically require invasive sample collection. This research presents a robust, non-enzymatic technique known as the Localized Hybridization Chain Reaction Amplifier (LHCRA), designed for real-time, in vivo miRNA imaging. This method addresses these limitations by providing rapid and sensitive detection of miRNAs, crucial for progressing clinical diagnostics and research. The LHCRA utilizes hairpin chain reaction probes embedded within self-assembled DNA micelles (DM), significantly amplifying miRNA signals. LHCRA demonstrated exceptional performance, with a detection limit of 100 pM and a response time of 10 min. Importantly, it effectively differentiated between cancerous and normal cells using clinical peripheral blood samples, showcasing high specificity and stability under physiological conditions. Additionally, LHCRA maintained consistent performance in complex biological media, including 10 % serum and 2 U/mL DNase I, confirming its broad applicability in various diagnostic scenarios. This performance highlights LHCRA's potential as a transformative tool in miRNA-based diagnostics. The introduction of LHCRA marks a significant advancement in miRNA detection technology. By enabling accurate, non-invasive, and real-time monitoring of miRNA dynamics within living cells, this method offers substantial potential to enhance diagnostic accuracy and deepen our understanding of disease mechanisms, particularly in cancer. Thus, it could greatly improve therapeutic strategies and patient outcomes in clinical environment. © 2024
Keyword ：
Diagnosis Diagnosis Diseases Diseases
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| GB/T 7714 | Yang, Bingyu , Gao, Xiang , Yu, Hengxin et al. Localized hybridization chain reaction amplifier utilizing three-dimensional DNA nanostructures for efficient and reliable microRNA imaging in living cells [J]. | Analytica Chimica Acta , 2024 , 1330 . |
| MLA | Yang, Bingyu et al. "Localized hybridization chain reaction amplifier utilizing three-dimensional DNA nanostructures for efficient and reliable microRNA imaging in living cells" . | Analytica Chimica Acta 1330 (2024) . |
| APA | Yang, Bingyu , Gao, Xiang , Yu, Hengxin , Xu, Jiawei , Liu, Wenming , Xu, Huo et al. Localized hybridization chain reaction amplifier utilizing three-dimensional DNA nanostructures for efficient and reliable microRNA imaging in living cells . | Analytica Chimica Acta , 2024 , 1330 . |
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燃香产生大量的颗粒物和挥发性物质已经成为室内空气污染的重要来源之一.目前有关燃香对局部大气环境影响的研究主要集中在燃香起源颗粒物浓度、挥发性气体、有机污染物等含量的测定.有关燃香所排放颗粒态重金属的排放率、排放因子以及所排放的颗粒态重金属对室内空气环境质量影响研究未见报道.使用微波消解仪对9种(S1—S9)线型藏香样品及其燃烧后所释放的总悬浮颗粒物进行程序升温消解,并采用电感耦合等离子体发射光谱(ICP-OES)分别在波长188.980,214.439,327.395及220.353 nm下对藏香及其燃烧所释放的总悬浮颗粒物中As,Cd,Cu和Pb含量进行检测,分析藏香燃后起源颗粒态重金属的排放特征,为评估室内藏香密集性使用所排放的颗粒态重金属对人体健康的潜在影响以及制定藏香重金属限量标准提供参考.实验发现:(1)不同种类藏香样品中四种重金属含量不同,平均含量从高到低依次为Cu,Pb,As和Cd元素.不同种类藏香样品中Cd和Pb的来源相似、而As和Cu的来源明显不同.(2)不同种类藏香燃烧后烟尘量不同,最高为94.75 mg·kg-1,最低为38.52 mg·kg-1.(3)不同藏香燃烧后的总悬浮颗粒物中As,Cd和Pb的排放量主要取决于它们在藏香样品中的含量,与藏香品种无关;而Cu在总悬浮颗粒物中的排放量受藏香品种影响较大,可能还受到其他因素的影响.(4)藏香燃烧后四种重金属的排放率(E)由高到低依次为Cd,As,Pb和Cu;而排放因子(F)由高到低依次As,Pb,Cd和Cu.(5)藏香燃后所排放的As和Cd可能对局部大气环境质量造成影响,其中来自同一厂家的样品S3,S6及S9的总悬浮颗粒物中As和Cd重金属含量均最高,建议藏香生产企业更加严格控制As和Cd等重金属的来源和含量,以降低藏香燃烧后对室内空气中重金属的污染,进而降低对人体健康危害的风险.
Keyword ：
排放因子 排放因子 排放率 排放率 藏香 藏香 重金属 重金属 颗粒物 颗粒物
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| GB/T 7714 | 陈萍芸 , 康秀棠 , 郭良洽 . 微波消解-电感耦合等离子体-发射光谱法研究藏香燃后起源颗粒态砷镉铜铅的排放特征 [J]. | 光谱学与光谱分析 , 2023 , 43 (2) : 419-425 . |
| MLA | 陈萍芸 et al. "微波消解-电感耦合等离子体-发射光谱法研究藏香燃后起源颗粒态砷镉铜铅的排放特征" . | 光谱学与光谱分析 43 . 2 (2023) : 419-425 . |
| APA | 陈萍芸 , 康秀棠 , 郭良洽 . 微波消解-电感耦合等离子体-发射光谱法研究藏香燃后起源颗粒态砷镉铜铅的排放特征 . | 光谱学与光谱分析 , 2023 , 43 (2) , 419-425 . |
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MoS2 nanosheets (NSs) have been found to possess the peroxidase-like activity and widely used in colorimetric sensors. However, the catalytic active sites of MoS2 NSs are still unclear. Herein, MoS2 NSs with sulfur defects were prepared using the molten salt method at different heating temperature (800 degrees C-1100 degrees C) and following by ultrasonic exfoliation. With the increasing of heating temperature, the crystallinity of MoS2 NSs is improved, accompanying with the decrement of S/Mo atomic ratio. The increment of sulfur defects in MoS2 NSs remarkably enhances the peroxidase-like activity of MoS2 NSs. The catalytic inhibition by phosphate-containing molecules further verifies the catalytic active sites of MoS2 NSs are Mo atoms with sulfur defects. Finally, the peroxidaselike activity of sulfur defect-rich MoS2 NSs was applied for the colorimetric detection of H2O2 and the inhibitor. This work helps to understand the peroxidase-like catalytic mechanism of MoS2 NSs.
Keyword ：
Active site Active site MoS 2 nanosheets MoS 2 nanosheets Peroxidase Peroxidase Pyrophosphate Pyrophosphate Sulfur defect Sulfur defect
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| GB/T 7714 | Xia, Chunqiu , Xu, Lanlan , Li, Zhihong et al. Sulfur defect-engineered MoS2 nanosheets with enhanced peroxidase-like activity [J]. | APPLIED SURFACE SCIENCE , 2023 , 635 . |
| MLA | Xia, Chunqiu et al. "Sulfur defect-engineered MoS2 nanosheets with enhanced peroxidase-like activity" . | APPLIED SURFACE SCIENCE 635 (2023) . |
| APA | Xia, Chunqiu , Xu, Lanlan , Li, Zhihong , Guo, Liangqia . Sulfur defect-engineered MoS2 nanosheets with enhanced peroxidase-like activity . | APPLIED SURFACE SCIENCE , 2023 , 635 . |
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Two-dimensional (2D) nanomaterials have been extensively used as quenching platforms of fluorescently labeled oligonucleotides for the design of biosensors. However, not all fluorophore-labeled single-stranded DNA (ssDNA) is necessarily quenched after they are adsorbed on the surface of 2D nanomaterials. Herein, we discovered that the fluorescence of tetramethylrhodamine (TMR)-labeled ssDNA was not quenched but enhanced by polymeric carbon nitride nanosheets (PCN NSs). Effects of the fluorophore, oligonucleotide sequence, and the number of proximal G bases on the fluorescence of fluorophore-labeled ssDNA were investigated in the presence of PCN NSs. The fluorescence enhancement phenomena were not observable for fluorescein- and X-rhodamine-labeled ssDNA and other 2D nanomaterials such as graphene oxide and MoS2 NSs. In the presence of PCN NSs, steric hindrance effect shields the photoinduced electron transfer quenching effect between TMR and proximal G bases, which may account for the fluorescence enhancement of TMR-labeled ssDNA adsorbed on PCN NSs. Benefiting from the fluorescence enhancement effect and the affinity change of PCN NSs to ssDNA probes upon hybridization with complementary ssDNA, a versatile ratiometric biosensing platform as a proof concept was demonstrated for the high sensitive fluorescence detection of ssDNA and microRNA.
Keyword ：
fluorescence enhancement fluorescence enhancement guanine base guanine base oligonucleotide probe oligonucleotide probe polymeric carbon nitride polymeric carbon nitride ratiometric sensor ratiometric sensor
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| GB/T 7714 | Chen, Jingru , Zhou, Dongshen , Chen, Zhifeng et al. Enhanced Polymeric Carbon Nitride Nanosheet-Based Fluorescence for Biosensing Applications [J]. | ACS APPLIED NANO MATERIALS , 2023 , 6 (2) : 1441-1447 . |
| MLA | Chen, Jingru et al. "Enhanced Polymeric Carbon Nitride Nanosheet-Based Fluorescence for Biosensing Applications" . | ACS APPLIED NANO MATERIALS 6 . 2 (2023) : 1441-1447 . |
| APA | Chen, Jingru , Zhou, Dongshen , Chen, Zhifeng , Liu, Xueting , Xu, Yuanteng , Guo, Liangqia . Enhanced Polymeric Carbon Nitride Nanosheet-Based Fluorescence for Biosensing Applications . | ACS APPLIED NANO MATERIALS , 2023 , 6 (2) , 1441-1447 . |
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Herein, a new strategy for laccase-like nanozyme is proposed by utilizing copper nanoparticles (Cu NPs) as the active center, carbon nitride as the skeleton and triazole groups as the cofactor. Cu NPs incorporated nitrogen -rich carbon nitride (Cu-g-C3N5) nanocomposites are found to possess excellent laccase-like activity, which can catalyze the oxidation of bisphenol A (BPA) to produce a color change in the presence of 4-aminoantipyrine. The catalytic efficiency of Cu-g-C3N5 nanocomposites is 4-fold higher than their analogue (Cu-g-C3N4 nano -composites) without triazole groups. Cu-g-C3N5 nanocomposites also display robust catalytic activity with high temperature stability, resistance to acid and alkalinity, and long term stability. Density functional theory cal-culations indicate that triazole groups are beneficial to improve the stability of Cu NPs via additional Cu-N bonds and facilitate the activation of the adsorbed O2 molecules. There is a linear relationship between the absorbance and BPA concentration over the range of 0.25-25 mg L-1 with the limit of detection of 0.09 mg L-1. Finally, Cu-g-C3N5 nanocomposites are successfully applied for colorimetric detection of BPA released from polycarbonate microplastics. To the best of our knowledge, this is the first example to utilize laccase-like nanozyme for colorimetric detection of BPA.
Keyword ：
Bisphenol A Bisphenol A Cofactor Cofactor Laccase Laccase Microplastics Microplastics Nanozyme Nanozyme
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| GB/T 7714 | Liu, Xiaotao , Wang, Fengxia , Xia, Chunqiu et al. Copper nanoparticles incorporated nitrogen-rich carbon nitride as laccase-like nanozyme for colorimetric detection of bisphenol a released from microplastics [J]. | MICROCHEMICAL JOURNAL , 2023 , 190 . |
| MLA | Liu, Xiaotao et al. "Copper nanoparticles incorporated nitrogen-rich carbon nitride as laccase-like nanozyme for colorimetric detection of bisphenol a released from microplastics" . | MICROCHEMICAL JOURNAL 190 (2023) . |
| APA | Liu, Xiaotao , Wang, Fengxia , Xia, Chunqiu , You, Qi , Chen, Xiaoxiao , Li, Yi et al. Copper nanoparticles incorporated nitrogen-rich carbon nitride as laccase-like nanozyme for colorimetric detection of bisphenol a released from microplastics . | MICROCHEMICAL JOURNAL , 2023 , 190 . |
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Large amounts of particulate and volatile matter derived from incense burning have become one of the important sources of indoor air pollution. At present, the research about the effect of burning incense on the local air environment mainly focused on determining particulate matters, volatile gases, and organic pollutants after incense burning. The research about the emission efficiencies and emission factors of heavy metals and the effect of particulate heavy metals on indoor air quality after incense burning has not been reported. To better understand the emission characteristics of heavy metals in Tibetan incense, nine kinds (S1S9) of line-type Tibetan incense powder samples and their total suspended particulates (TSP) after burning were digested by microwave digestion method with programmed temperature procedure, and four primary kinds of heavy metal elements (i.e. As, Cd, Cu, Pb) in these powder samples. Their TSP were determined by the inductively coupled plasma optical emission spectrometry (ICP-OES) with the wavelength 188.980 nm for As, 214.439 nm for Cd, 327.395 nm for Cu and 220.353 nm for Pb, respectively. The experiment results are listed as follows: (1) The contents of four heavy metals are different in different Tibetan incense samples, and the average content of heavy metal from high to low is in the order of Cu, Pb, As and Cd. The source of Cd and Pb in different Tibetan incense samples is similar, remarkably different from As and Cu. (2) After burning, the amounts of smoke and dust from different Tibetan incense samples are different. The largest and minimum amounts are 94.75 and 38.52 mg center dot kg(-1), respectively. (3) The emission amounts of As, Cd and Pb in the total suspended particles from different Tibetan incense samples depend on their contents in the incense sample. However, the emission amount of Cu is not only dependent on the incense samples but also affected by other factors. (4) The emission efficiency of heavy metals in Tibetan incense samples from high to low is in the order of Cd, As, Pb, and Cu. The emission factor from high to low is in the order of As, Pb, Cd, and Cu. (5) The emission of As and Cd from Tibetan incense samples after burning may adversely affect local atmosphere quality. Among these samples, the highest contents of As and Cd in the TSP of S3, S5 and S9 from the same manufacturer. The manufacturer is suggested to control the source and content of As and Cd stricter to decrease the heavy metal pollution in indoor air and the risk to human health after burning Tibetan incenses.
Keyword ：
Emission efficiency Emission efficiency Emission factor Emission factor Heavy metals Heavy metals Tibetan incense Tibetan incense Total suspended particles Total suspended particles
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| GB/T 7714 | Chen Ping-yun , Kang Xiu-tang , Guo Liang-qia . Study of Emission Characteristics of Particulate Arsenic, Cadmium, Copper and Lead Derived From Burning of Tibetan Incenses by ICP-OES Method With Microwave Digestion [J]. | SPECTROSCOPY AND SPECTRAL ANALYSIS , 2023 , 43 (2) : 419-425 . |
| MLA | Chen Ping-yun et al. "Study of Emission Characteristics of Particulate Arsenic, Cadmium, Copper and Lead Derived From Burning of Tibetan Incenses by ICP-OES Method With Microwave Digestion" . | SPECTROSCOPY AND SPECTRAL ANALYSIS 43 . 2 (2023) : 419-425 . |
| APA | Chen Ping-yun , Kang Xiu-tang , Guo Liang-qia . Study of Emission Characteristics of Particulate Arsenic, Cadmium, Copper and Lead Derived From Burning of Tibetan Incenses by ICP-OES Method With Microwave Digestion . | SPECTROSCOPY AND SPECTRAL ANALYSIS , 2023 , 43 (2) , 419-425 . |
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Nanozymes have attracted great interest in the field of biotechnology, (bio)sensing, and environmental monitoring. Herein, a co-doping strategy is proposed to enhance the peroxidase-like activity of graphitic carbon nitride (g-C3N4). Cu2+ and triazole group co-doped graphitic carbon nitride (g-C3N5-Cu2+) was derived from thermal polymerization of 3-amino-1,2,4-triazole and then coordination with Cu2+. g-C3N5-Cu2+ nanosheets (NSs) could catalyze the oxidation reaction of 3,3 ',5,5 '-tetramethylbenzidine (TMB) by H2O2 under neutral conditions, yielding 10.9-fold better catalytic activity than g-C3N4 NSs. Reactive oxygen species scavenging experiments indicated that (OH)-O-center dot played a crucial role during the catalytic process. When glutathione (GSH) was introduced, the absorbance of the system decreased because of the reduction of the oxidation product of TMB by GSH. The colorimetric method demonstrated a sensitive response for GSH with a linear range of 0.8-33.3 mu mol L-1 and a limit of detection of 0.3 mu mol L-1. The sensitivity is higher than most other peroxidase mimetic-based colorimetric methods for GSH. Finally, the feasibility for the detection of GSH in HeLa cells was demonstrated.
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| GB/T 7714 | Liu, Xiaotao , Zheng, Xueyi , Xia, Chunqiu et al. Graphitic carbon nitride with Cu2+ and triazole group co-doping for enhanced peroxidase-like activity and its application for glutathione detection [J]. | SENSORS & DIAGNOSTICS , 2023 , 2 (4) : 902-908 . |
| MLA | Liu, Xiaotao et al. "Graphitic carbon nitride with Cu2+ and triazole group co-doping for enhanced peroxidase-like activity and its application for glutathione detection" . | SENSORS & DIAGNOSTICS 2 . 4 (2023) : 902-908 . |
| APA | Liu, Xiaotao , Zheng, Xueyi , Xia, Chunqiu , Guo, Liangqia . Graphitic carbon nitride with Cu2+ and triazole group co-doping for enhanced peroxidase-like activity and its application for glutathione detection . | SENSORS & DIAGNOSTICS , 2023 , 2 (4) , 902-908 . |
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