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学者姓名：许雪琴
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Abstract ：
In this paper, a novel and sensitive electrochemical aptasensor for sulfadimethoxine (SDM) detection has been designed based on the triple helix structure/exonuclease I (Exo I)-assisted double signal amplification strategy. The aptamer probe (Apt) hybridizes with the signal transduction probe (STP) on the electrode to form a rigid double-stranded DNA (dsDNA) structure, so that the STP remains upright and methylene blue (MB) on the STP is far away from the electrode surface, resulting in a delicate current signal. In the presence of SDM, the SDM and Apt combine into a complex, leading to the transfer of the Apt and the exposure of the STP. Meanwhile, the added Exo I can digest the Apt to realize the cyclic amplification of SDM. After the addition of the signal probe (SP), a triple helix structure between the SP and STP is formed under acidic conditions, and MB on the STP and SP collide with the electrode surface to generate a strong electrochemical signal. The proposed aptasensor combines the features of the triple helix structure and Exo I to achieve double signal amplification for the sensitive detection of SDM with a wide linear range of 0.05-1000 ng mL-1 and a low detection limit of 0.02 ng mL-1. Furthermore, it has been successfully used to detect SDM in milk and lake water samples. The triple helix/Exo I-assisted double-amplification strategy effectively improves the sensitivity of the aptasensor. The aptasensor has simple operation and strong specificity and has been applied to detect sulfadimethoxine (SDM) in real samples.
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| GB/T 7714 | Du, Meijuan , Cheng, Xin , Chen, Qian et al. A novel and sensitive electrochemical aptasensor for sulfadimethoxine detection based on the triple helix/exonuclease I-assisted double-amplification strategy [J]. | ANALYTICAL METHODS , 2024 , 16 (11) : 1570-1578 . |
| MLA | Du, Meijuan et al. "A novel and sensitive electrochemical aptasensor for sulfadimethoxine detection based on the triple helix/exonuclease I-assisted double-amplification strategy" . | ANALYTICAL METHODS 16 . 11 (2024) : 1570-1578 . |
| APA | Du, Meijuan , Cheng, Xin , Chen, Qian , Xu, Xueqin . A novel and sensitive electrochemical aptasensor for sulfadimethoxine detection based on the triple helix/exonuclease I-assisted double-amplification strategy . | ANALYTICAL METHODS , 2024 , 16 (11) , 1570-1578 . |
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A ratiometric electrochemical aptasensor based on gold nanoparticles (AuNPs) functionalization and hybridization chain reaction (HCR) assisted signal amplification has been for the first time designed for the detection of streptomycin (STR). The double-stranded DNA (dsDNA) formed by the hybridization of ferrocene (Fc)-labeled STR aptamer (Apt) and capture probe (CP) is first immobilized on the gold electrode (GE) surface via Au-S reaction. The specific binding of the target and Apt results in numerous Fc detachment from the sensing interface. Then, the remaining single-stranded CP is combined with AuNPs modified with initiator DNA (iDNA) by auxiliary DNA (aDNA). Among them, the iDNA triggers HCR between two hairpin probes (H1/H2), thus capturing a large number of methylene blue (MB) electrochemical probe, which generates a strong electrochemical signal of MB and a weak electrochemical signal of Fc. Signals are collected by square wave voltammetry (the potential window ranging from -0.5 V to 0.6 V, vs. Ag/AgCl ), and the oxidation peak currents at -0.200 V (MB) and 0.416 V (Fc) are recorded. The use of the ratiometric method has effectively improved the accuracy and reliability of the analysis. The successful application of AuNPs and HCR greatly improves the sensitivity of the sensor, and the detection limit is as low as 0.08 pM. It can sensitively determine STR in the range 0.1 pM to 10 nM. In addition, the designed aptasensor has been successfully applied to the detection of STR in milk and honey samples.
Keyword ：
Gold nanoparticles Gold nanoparticles Hybridization chain reaction Hybridization chain reaction Ratiometric electrochemical aptasensor Ratiometric electrochemical aptasensor Signal amplification Signal amplification Square wave voltammetry Square wave voltammetry Streptomycin Streptomycin
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| GB/T 7714 | Zhang, Zhoubing , Jia, Xiaorun , Xu, Xueqin . An electrochemical aptasensor for detection of streptomycin based on signal amplification assisted by functionalized gold nanoparticles and hybridization chain reaction [J]. | MICROCHIMICA ACTA , 2023 , 190 (4) . |
| MLA | Zhang, Zhoubing et al. "An electrochemical aptasensor for detection of streptomycin based on signal amplification assisted by functionalized gold nanoparticles and hybridization chain reaction" . | MICROCHIMICA ACTA 190 . 4 (2023) . |
| APA | Zhang, Zhoubing , Jia, Xiaorun , Xu, Xueqin . An electrochemical aptasensor for detection of streptomycin based on signal amplification assisted by functionalized gold nanoparticles and hybridization chain reaction . | MICROCHIMICA ACTA , 2023 , 190 (4) . |
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A novel and label-free electrochemical aptasensor based on exonuclease III (Exo III) and G-quadruplex DNAzyme has been first designed to detect metronidazole (MNZ) sensitively and selectively in this paper. Capture probe (CP), helper probe (HP) and aptamer (Apt) hybridize to form double-stranded DNA (dsDNA) structure with a blunt 3'-terminus, resulting in the guanine (G)-rich CP being enclosed in the dsDNA, followed by specific digestion of the 3 & PRIME;-terminus by Exo III to expose the G-rich sequence. At this time, CP can bind with the added hemin to form G-quadruplex DNAzyme which catalyzes the redox reaction of 3,3',5,5'-tetramethylbenzidine (TMB) and H2O2, generating a strong current signal. However, in the presence of MNZ, the binding of MNZ and aptamer breaks the original blunt 3 & PRIME;-terminus of the dsDNA structure, blocking the subsequent series of reactions, and only a weak current signal can be observed. Under the optimal conditions, the aptasensor showed excellent sensitivity and specificity for the detection of MNZ with a wide linear range from 10 pM to 500 nM and a detection limit as low as 3.0 pM (S/N = 3). The aptasensor constructed based on MNZ aptamer can effectively avoid the interference of other nitro compounds and antibiotics to a certain extent. Compared with other specific recognition elements, the aptasensor possesses the advantages of strong specificity, simple preparation and low cost. Moreover, the proposed aptasensor has been successfully applied to the detection of MNZ in tap water and honey samples.
Keyword ：
Electrochemical aptasensor Electrochemical aptasensor Exo III Exo III G-quadruplex DNAzyme G-quadruplex DNAzyme Metronidazole Metronidazole
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| GB/T 7714 | Du, Meijuan , Chen, Qian , Xu, Xueqin . A novel and label-free electrochemical aptasensor based on exonuclease III and G-quadruplex DNAzyme for sensitive and selective detection of metronidazole [J]. | MICROCHEMICAL JOURNAL , 2022 , 179 . |
| MLA | Du, Meijuan et al. "A novel and label-free electrochemical aptasensor based on exonuclease III and G-quadruplex DNAzyme for sensitive and selective detection of metronidazole" . | MICROCHEMICAL JOURNAL 179 (2022) . |
| APA | Du, Meijuan , Chen, Qian , Xu, Xueqin . A novel and label-free electrochemical aptasensor based on exonuclease III and G-quadruplex DNAzyme for sensitive and selective detection of metronidazole . | MICROCHEMICAL JOURNAL , 2022 , 179 . |
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Di(2-ethylhexyl) phthalate (DEHP) is an endocrine disrupting compound that is often used as a plasticizer and tends to migrate in the environment and accumulates in the human body causing harm to humans. Thus, a simple, label-free, enzyme-free, high selective and ultrasensitive electrochemical aptasensor has been constructed for detection of DEHP based on the self-assembly of DNA nanostructure signal amplification strategy in this paper. The DNA-junction nanostructure is made of hairpin 1 (HP1), hairpin 2 (HP2), hairpin 3 (HP3) and quarter sequence (QS). Without DEHP, the electrochemical detection response is very low. Only when the aptamer binds to the target and detaches from the electrode surface, the DNA-junction is trapped on the electrode surface, enhancing the electrochemical signal. This is because the DNA-junction nanostructure is rich in guanine, which is able to adsorb more methylene blue (MB) for the purpose of enhancing the detection signal. The linear detection range of the obtained aptasensor is 0.1 ng mL(-1) to 5000 ng mL(-1) and the limit detection is 0.04 ng mL(-1). Finally, the proposed aptasensor has been applied to analyze DEHP in real samples, laying the foundation for its utilization in environmental water samples and food safety.
Keyword ：
Di(2-ethylhexyl) phthalate Di(2-ethylhexyl) phthalate DNA-junction DNA-junction Electrochemical aptasensor Electrochemical aptasensor Nanostructure Nanostructure
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| GB/T 7714 | Chen, Qian , Du, Meijuan , Xu, Xueqin . A label-free and selective electrochemical aptasensor for ultrasensitive detection of Di(2-ethylhexyl) phthalate based on self-assembled DNA nanostructure amplification [J]. | JOURNAL OF ELECTROANALYTICAL CHEMISTRY , 2022 , 914 . |
| MLA | Chen, Qian et al. "A label-free and selective electrochemical aptasensor for ultrasensitive detection of Di(2-ethylhexyl) phthalate based on self-assembled DNA nanostructure amplification" . | JOURNAL OF ELECTROANALYTICAL CHEMISTRY 914 (2022) . |
| APA | Chen, Qian , Du, Meijuan , Xu, Xueqin . A label-free and selective electrochemical aptasensor for ultrasensitive detection of Di(2-ethylhexyl) phthalate based on self-assembled DNA nanostructure amplification . | JOURNAL OF ELECTROANALYTICAL CHEMISTRY , 2022 , 914 . |
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A rapid and simple method was developed to determine the phenolic acids ferulic acid, chlorogenic acid and salicylic acid in botanical drugs by capillary electrophoresis with chemiluminescence detection. The sensitive chemiluminescence detection was carried out in a laboratory-constructed temperature-controlled interface by the use of acidic potassium permanganate as a chemiluminescence reagent. To obtain the optimized conditions, the experimental conditions for the capillary electrophoresis separation and chemiluminescence detection were systematically examined. Under the optimized conditions, the analytes were well separated within 7 min. The limits of detection for ferulic acid, chlorogenic acid and salicylic acid were 35, 58 and 37 mu g/mL and the limits of quantification were 117, 193 and 123 mu g/mL, respectively. The proposed method was successfully applied for the quantitative determination of phenolic acids inLonicera japonicaflos and Rhizoma Chuanxiong.
Keyword ：
botanical drugs botanical drugs Capillary electrophoresis Capillary electrophoresis chemiluminescence chemiluminescence phenolic acids phenolic acids
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| GB/T 7714 | Chen, Xin , Mao, Jingxiu , Wen, Fuyu et al. Determination of Phenolic Acids in Botanical Pharmaceutical Products by Capillary Electrophoresis with Chemiluminescence Detection [J]. | ANALYTICAL LETTERS , 2020 . |
| MLA | Chen, Xin et al. "Determination of Phenolic Acids in Botanical Pharmaceutical Products by Capillary Electrophoresis with Chemiluminescence Detection" . | ANALYTICAL LETTERS (2020) . |
| APA | Chen, Xin , Mao, Jingxiu , Wen, Fuyu , Xu, Xueqin . Determination of Phenolic Acids in Botanical Pharmaceutical Products by Capillary Electrophoresis with Chemiluminescence Detection . | ANALYTICAL LETTERS , 2020 . |
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T4 polynucleotide kinase (T4 PNK), a bifunctional repair enzyme, catalyzes the transfer of gamma-phosphate residues from adenosine triphosphate (ATP) to 5'- hydroxyl termini in nucleic acids square which plays an essential role in regulating DNA phosphorylation modes during the repair of damaged DNA. Herein, a sensitive DNAzyme-driven DNA walker biosensor was developed for amplified electrochemical detection of T4 PNK activity and inhibition based on T4 PNK-triggered phosphorylation reaction and lambda exonuclease (lambda exo) cleavage. A novel functional hairpin-shaped swing arm DNA (SA) containing a DNA sequence of the Pb2+-dependent DNAzyme is designed to specifically bind to and hydrolyze the substrate strand (rSS) sequence that can drive DNA walker. Upon the reaction of SA square T4 PNK and ATP, lambda exo removes the mononucleotides from the stem of the phosphorylated SA, unfolding the hairpin structure and initiating DNAzyme-driven DNA walker. Subsequently, the DNA walker can repeatedly bind and cleave rSS with the aid of Pb2+, releasing the methylene blue (MB) - labeled fragment of rSS. The T4 PNK activity can be facilely monitored by recording the electrochemical signal change of MB. The proposed biosensor exhibited a broad linear range from 0.01 U mL(-1 )to 15 U mL(-1) with a low detection limit of 0.001 U mL(-1). The proposed biosensor has also been utilized to screen the T4 PNK inhibitors and exhibited good performance in real sample analysis, making such a strategy hold potential promise in biological fields and clinical diagnosis.
Keyword ：
DNA walker biosensor DNA walker biosensor DNAzyme-driven DNAzyme-driven Electrodiemical detection Electrodiemical detection Inhibition Inhibition T4 polynucleotide kinase T4 polynucleotide kinase
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| GB/T 7714 | Mao, Jingxiu , Chen, Xin , Xu, Huahuang et al. DNAzyme-driven DNA walker biosensor for amplified electrochemical detection of T4 polynucleotide kinase activity and inhibition [J]. | JOURNAL OF ELECTROANALYTICAL CHEMISTRY , 2020 , 874 . |
| MLA | Mao, Jingxiu et al. "DNAzyme-driven DNA walker biosensor for amplified electrochemical detection of T4 polynucleotide kinase activity and inhibition" . | JOURNAL OF ELECTROANALYTICAL CHEMISTRY 874 (2020) . |
| APA | Mao, Jingxiu , Chen, Xin , Xu, Huahuang , Xu, Xueqin . DNAzyme-driven DNA walker biosensor for amplified electrochemical detection of T4 polynucleotide kinase activity and inhibition . | JOURNAL OF ELECTROANALYTICAL CHEMISTRY , 2020 , 874 . |
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设计了一种基于Ag纳米信号和限制性核酸外切酶Ⅲ(Exo Ⅲ)辅助的mi-RNA检测生物传感器。该传感器将银纳米直接沉积在金电极表面,在实现放大金电极表面可接触位点的同时,完成对电化学氧化还原检测信号的加载。目标检测物mi-RNA与电极表面加载的捕获探针进行双链杂交,对银纳米与检测缓冲液间的电荷交换行为产生抑制,加入Exo Ⅲ对双链结构剪切,改变电荷交换过程中的抑制作用强弱,进而实现目标检测物与检测信号间的线性关系。该传感器对mi-RNA的检测线性范围为1.0×10~(-18)-1.0×10~(-10)M,检测限为6.1×10~(-4)fM。
Keyword ：
Exo Ⅲ Exo Ⅲ mi-RNA mi-RNA 生物传感器 生物传感器 银纳米 银纳米
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| GB/T 7714 | 杜梅娟 , 李明玉 , 许雪琴 . 基于银纳米信号和限制性核酸外切酶Ⅲ辅助的mi-RNA检测生物传感器 [C] //中国化学会第十四届全国电分析化学学术会议会议论文集（第二分册） . 2020 . |
| MLA | 杜梅娟 et al. "基于银纳米信号和限制性核酸外切酶Ⅲ辅助的mi-RNA检测生物传感器" 中国化学会第十四届全国电分析化学学术会议会议论文集（第二分册） . (2020) . |
| APA | 杜梅娟 , 李明玉 , 许雪琴 . 基于银纳米信号和限制性核酸外切酶Ⅲ辅助的mi-RNA检测生物传感器 中国化学会第十四届全国电分析化学学术会议会议论文集（第二分册） . (2020) . |
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本文设计了一种基于Lambda核酸外切酶(λExo)的T4多聚核苷酸激酶(T4 PNK)检测生物传感器。利用HCR策略在金电极表面加载5'-OH/3'-OH型DNA长双链结构;而后,加入5'-三磷酸腺苷(ATP)及目标检测物T4 PNK,在ATP的存在下,T4 PNK可将5'-OH/3'-OH型长DNA双链结构转化为5'-PO4/3'-OH型;此时加入特异切割5'-PO4/3'-OH型双链DNA结构的λExo,金电极表面的长DNA双链结构被切割减少,再在剩余DNA结构上加载亚甲基蓝作为电化学响应信号。该传感器检测T4PNK的线性范围为0.0001-30.0 UmL~(-1),检测限为2.8x10~(-5)UmL~(-1)。
Keyword ：
DNA磷酸化 DNA磷酸化 T4PNK T4PNK 生物传感器 生物传感器
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| GB/T 7714 | 陈倩 , 李明玉 , 许雪琴 . 基于Lambda核酸外切酶辅助的信号放大型T4多聚核苷酸激酶检测生物传感器 [C] //中国化学会第十四届全国电分析化学学术会议会议论文集（第二分册） . 2020 . |
| MLA | 陈倩 et al. "基于Lambda核酸外切酶辅助的信号放大型T4多聚核苷酸激酶检测生物传感器" 中国化学会第十四届全国电分析化学学术会议会议论文集（第二分册） . (2020) . |
| APA | 陈倩 , 李明玉 , 许雪琴 . 基于Lambda核酸外切酶辅助的信号放大型T4多聚核苷酸激酶检测生物传感器 中国化学会第十四届全国电分析化学学术会议会议论文集（第二分册） . (2020) . |
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An ultrasensitive conformation-dependent colorimetric assay has been developed for the detection of mercury(II) ions. It is based on the use of exonuclease III (Exo III)-assisted target recycling and gold nanoparticles (AuNPs). In the absence of Hg(II), the hairpin-shaped DNA probe (H-DNA) binds to AuNPs and stabilizes them in solutions of high ionic strength. In the presence of Hg(II), on the other hand, the sticky termini of the H-DNA form a rigid DNA duplex stem with a blunt 3'-terminus. Thus, Exo III is activated as a biocatalyst for selective and stepwise removal of mononucleotides from the 3'-terminus of the H-DNA. As a result, Hg(II) is released from the T-Hg(II)-T complexes. The guanine-rich sequences released from the H-DNA are then self-assembled with potassium ion to form a stable G-quadruplex conformation. In solutions of high ionic strength, this results in aggregation of AuNPs and a color change from red to blue which can be seen with bare eyes. The method is highly sensitive and selective. It has a linear response in the 10 pM to 100 nM Hg(II) concentration range, and the detection limit is as low as 3.2 pM (at an S/N ratio of 3). The relative standard deviation at a level of 0.5 nM of Hg(II) is 4.9% (for n = 10). The method was applied to the detection of Hg(II) in spiked environment water samples, with recoveries ranging from 92% to 106%.
Keyword ：
AuNPs AuNPs Colorimetric method Colorimetric method DNA probe DNA probe Environmental water analysis Environmental water analysis Exo III Exo III G-quadruplex G-quadruplex Hg(II) Hg(II) TEM TEM UV-Vis spectrophotometry UV-Vis spectrophotometry Visual detection Visual detection
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| GB/T 7714 | Hong, Minqiang , Zeng, Bihua , Li, Mingyu et al. An ultrasensitive conformation-dependent colorimetric probe for the detection of mercury(II) using exonuclease III-assisted target recycling and gold nanoparticles [J]. | MICROCHIMICA ACTA , 2018 , 185 (1) . |
| MLA | Hong, Minqiang et al. "An ultrasensitive conformation-dependent colorimetric probe for the detection of mercury(II) using exonuclease III-assisted target recycling and gold nanoparticles" . | MICROCHIMICA ACTA 185 . 1 (2018) . |
| APA | Hong, Minqiang , Zeng, Bihua , Li, Mingyu , Xu, Xueqin , Chen, Guonan . An ultrasensitive conformation-dependent colorimetric probe for the detection of mercury(II) using exonuclease III-assisted target recycling and gold nanoparticles . | MICROCHIMICA ACTA , 2018 , 185 (1) . |
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In this paper, a novel and signal-on electrochemical biosensor based on Hg2+- triggered nicking endonucleaseassisted target recycling and hybridization chain reaction (HCR) amplification tactics was developed for sensitive and selective detection of Hg2+. The hairpin-shaped capture probe A (PA) contained a specific sequence which was recognized by nicking endonuclease (NEase). In the presence of Hg2+, probe B (PB) hybridized with PA to form stand-up duplex DNA strands via the Hg2+ mediated thymine-Hg2+-thymine (T-Hg2+-T) structure, which automatically triggered NEase to selectively digest duplex region from the recognition sites, spontaneously dissociating PB and Hg2+ and leaving the remnant initiators. The released PB and Hg2+ could be reused to initiate the next cycle and more initiators were generated. The long nicked double helices were formed through HCR event, which was triggered by the initiators and two hairpin-shaped signal probes labeled with methylene blue, resulting in a significant signal increase. Under optimum conditions, the resultant biosensor showed the high sensitivity and selectivity for the detection of Hg2+ in a linear range from 10 pM to 50 nM (R-2=0.9990), and a detection limit as low as 1.6 pM (S/N=3). Moreover, the proposed biosensor was successfully applied in the detection of Hg2+ in environment water samples with satisfactory results.
Keyword ：
Electrochemical biosensor Electrochemical biosensor Hg2+ detection Hg2+ detection Hybridization chain reaction Hybridization chain reaction Nicking endonuclease Nicking endonuclease
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| GB/T 7714 | Hong, Minqiang , Wang, Mengyan , Wang, Jing et al. Ultrasensitive and selective electrochemical biosensor for detection of mercury (II) ions by nicking endonuclease-assisted target recycling and hybridization chain reaction signal amplification [J]. | BIOSENSORS & BIOELECTRONICS , 2017 , 94 : 19-23 . |
| MLA | Hong, Minqiang et al. "Ultrasensitive and selective electrochemical biosensor for detection of mercury (II) ions by nicking endonuclease-assisted target recycling and hybridization chain reaction signal amplification" . | BIOSENSORS & BIOELECTRONICS 94 (2017) : 19-23 . |
| APA | Hong, Minqiang , Wang, Mengyan , Wang, Jing , Xu, Xueqin , Lin, Zhenyu . Ultrasensitive and selective electrochemical biosensor for detection of mercury (II) ions by nicking endonuclease-assisted target recycling and hybridization chain reaction signal amplification . | BIOSENSORS & BIOELECTRONICS , 2017 , 94 , 19-23 . |
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