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学者姓名：陈剑锋
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为实现鱿鱼加工下脚料鱿鱼皮的高值化利用，本研究采用酶法制备鱿鱼皮蛋白肽。以多肽浓度和三氯乙酸可溶性氮指数（Trichloroacetic acid-nitrogen soluble index，TCA-NSI）为评价指标，采用单因素和响应面试验优化酶解工艺参数，并对所制备蛋白肽的氨基酸组成、分子量分布、蛋白肽序列、抗氧化活性及其体外模拟消化特性进行表征。结果表明，最佳酶解工艺条件为酶解温度43.50 ℃、酶解时间100 min、酶解pH7.50、胰蛋白酶与碱性蛋白酶比例2:1、加酶量4000 U/g，在此条件下其TCA-NSI可达89.02%±0.66%。所制备的鱿鱼皮蛋白肽其氨基酸组成均衡，必需氨基酸指数（Essential amino acid index，EAAI）可达0.90。此外，鱿鱼皮蛋白肽含有43个多肽序列，其中38个肽段的分子质量低于1800 Da，其DPPH自由基、ABTS+自由基和羟自由基清除率的IC50分别为0.61、0.28和1.95 mg/mL，且经体外模拟胃肠消化后仍能维持较好的抗氧化活性。本研究结果为鱿鱼加工下脚料的开发利用和精深加工提供了理论依据。
Keyword ：
体外模拟消化 体外模拟消化 抗氧化活性 抗氧化活性 理化性质 理化性质 蛋白肽 蛋白肽 鱿鱼皮 鱿鱼皮
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| GB/T 7714 | 朱清清 , 马瑞娟 , 陈剑锋 et al. 响应面法优化鱿鱼皮蛋白肽的制备工艺及其理化性质分析 [J]. | 食品工业科技 , 2024 : 1-10 . |
| MLA | 朱清清 et al. "响应面法优化鱿鱼皮蛋白肽的制备工艺及其理化性质分析" . | 食品工业科技 (2024) : 1-10 . |
| APA | 朱清清 , 马瑞娟 , 陈剑锋 , 谢友坪 . 响应面法优化鱿鱼皮蛋白肽的制备工艺及其理化性质分析 . | 食品工业科技 , 2024 , 1-10 . |
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Polyethylene terephthalate (PET) is a polymer synthesized via the dehydration and condensation reaction between ethylene glycol and terephthalic acid. PET has emerged as one of the most extensively employed plastic materials due to its exceptional plasticity and durability. Nevertheless, PET has a complex structure and is extremely difficult to degrade in nature, causing severe pollution to the global ecological environment and posing a threat to human health. Currently, the methods for PET processing mainly include physical, chemical, and biological methods. Biological enzyme degradation is considered the most promising PET degradation method. In recent years, an increasing number of enzymes that can degrade PET have been identified, and they primarily target the ester bond of PET. This review comprehensively introduced the latest research progress in PET enzymatic degradation from the aspects of PET-degrading enzymes, PET biodegradation pathways, the catalytic mechanism of PET-degrading enzymes, and biotechnological strategies for enhancing PET-degrading enzymes. On this basis, the current challenges within the enzymatic PET degradation process were summarized, and the directions that need to be worked on in the future were pointed out. This review provides a reference and basis for the subsequent effective research on PET biodegradation.
Keyword ：
Biodegradation pathway Biodegradation pathway Bioengineering modification Bioengineering modification Catalytic mechanism Catalytic mechanism PET-degrading enzyme PET-degrading enzyme
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| GB/T 7714 | Qiu, Jiarong , Chen, Yuxin , Zhang, Liangqing et al. A comprehensive review on enzymatic biodegradation of polyethylene terephthalate [J]. | ENVIRONMENTAL RESEARCH , 2024 , 240 . |
| MLA | Qiu, Jiarong et al. "A comprehensive review on enzymatic biodegradation of polyethylene terephthalate" . | ENVIRONMENTAL RESEARCH 240 (2024) . |
| APA | Qiu, Jiarong , Chen, Yuxin , Zhang, Liangqing , Wu, Jinzhi , Zeng, Xianhai , Shi, Xinguo et al. A comprehensive review on enzymatic biodegradation of polyethylene terephthalate . | ENVIRONMENTAL RESEARCH , 2024 , 240 . |
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To achieve the high-value utilization of squid skin obtained from squid processing, this study utilized an enzymatic approach to prepare protein peptides. The enzymatic hydrolysis parameters were optimized using single factor and response surface tests, with the concentration of peptide and trichloroacetic acid-nitrogen soluble index (TCA-NSI) serving as evaluation criteria. The amino acid composition, molecular weight distribution, peptide sequence, antioxidant activity, and in vitro simulated digestive properties of the prepared protein peptides were also thoroughly characterized. The results showed that the optimal conditions for enzymatic hydrolysis were as follows: Enzymatic temperature of 43.50 ℃, enzymatic time of 100 minutes, enzymatic pH of 7.50, trypsin to alkaline protease ratio of 2:1, and enzyme dosage of 4000 U/g. Under these specified conditions, the TCA-NSI achieved a value of 89.02%±0.66%. The prepared protein peptides exhibited a well-balanced amino acid composition, with an essential amino acid index (EAAI) value of 0.90. Furthermore, the prepared protein peptides encompassed a total of 43 polypeptide sequences, with 38 of these segments exhibiting molecular weights below 1800 Da. The IC50 values for scavenging DPPH radical, ABTS+ radical, and hydroxyl radical were determined to be 0.61, 0.28 and 1.95 mg/mL, respectively. Notably, the antioxidant activity remained favorable even after undergoing simulated gastrointestinal digestion in vitro. These findings serve to establish a solid theoretical basis for the advancement and exploitation of byproducts derived from squid processing, as well as their subsequent value-added utilization. © 2024 Editorial Department of Science and Technology of Food Science. All rights reserved.
Keyword ：
Alkalinity Alkalinity Amino acids Amino acids Antioxidants Antioxidants Enzymatic hydrolysis Enzymatic hydrolysis Molecular weight distribution Molecular weight distribution Peptides Peptides Physicochemical properties Physicochemical properties Shellfish Shellfish Trichloroacetic acid Trichloroacetic acid
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| GB/T 7714 | Qingqing, Zhu , Ruijuan, Ma , Jianfeng, Chen et al. Optimization of Preparation Process of Squid Skin Peptides by Response Surface Methodology and Its Physicochemical Properties [J]. | Science and Technology of Food Industry , 2024 , 45 (7) : 217-226 . |
| MLA | Qingqing, Zhu et al. "Optimization of Preparation Process of Squid Skin Peptides by Response Surface Methodology and Its Physicochemical Properties" . | Science and Technology of Food Industry 45 . 7 (2024) : 217-226 . |
| APA | Qingqing, Zhu , Ruijuan, Ma , Jianfeng, Chen , Youping, Xie . Optimization of Preparation Process of Squid Skin Peptides by Response Surface Methodology and Its Physicochemical Properties . | Science and Technology of Food Industry , 2024 , 45 (7) , 217-226 . |
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Combination of taxa and function can provide a more comprehensive picture on human-induced microbial homogenization. Here, we obtained 2.58 billion high-throughput sequencing reads and 479 high-quality metagenome-assembled genomes (MAGs) of planktonic microbial communities in a subtropical river for 5 years. We found the microbial taxa homogenization and functional homogenization were uncoupled. Although human activities in downstream sites significantly decreased the taxonomic diversity of non-abundant ASV communities (16S rRNA gene amplicon sequence variants), they did not significantly decrease the taxonomic diversity of abundant ASV and total observed MAG communities. However, the total observed MAG communities in downstream sites tended to homogenize into some specific taxa which encode human-activity-related functional genes, such as nutrient cycles, greenhouse gas emission, antibiotic and arsenic resistance. Those specific MAGs with high taxonomic diversity caused the weak heterogenization of total observed MAG communities in downstream sites. Moreover, functional homogenization promoted the synchrony among downstream MAGs, and these MAGs constructed some specific network modules might to synergistically execute or resist the humanactivity-related functions. High synchrony also led to the tandem effects among MAGs and thus decreased community stability. Overall, our findings revealed the links of microbial taxa, functions and stability under human activity impacts, and provided a strong evidence to encourage us re-thinking biotic homogenization based on microbial taxa and their functional attributes.
Keyword ：
Biotic homogenization Biotic homogenization Human activities Human activities Metagenome Metagenome Microbial community Microbial community Nitrogen cycle Nitrogen cycle
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| GB/T 7714 | Liu, Lemian , Wang, Hongwei , Guo, Yisong et al. Human-induced homogenization of microbial taxa and function in a subtropical river and its impacts on community stability [J]. | WATER RESEARCH , 2024 , 252 . |
| MLA | Liu, Lemian et al. "Human-induced homogenization of microbial taxa and function in a subtropical river and its impacts on community stability" . | WATER RESEARCH 252 (2024) . |
| APA | Liu, Lemian , Wang, Hongwei , Guo, Yisong , Yan, Qi , Chen, Jianfeng . Human-induced homogenization of microbial taxa and function in a subtropical river and its impacts on community stability . | WATER RESEARCH , 2024 , 252 . |
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戊二醇（1,2-戊二醇、1,4-戊二醇及1,5-戊二醇等）是全球广泛使用的重要医药化工原料，不仅可以用作杀菌剂丙环唑的中间体和化妆品配方，还是生产表面活性剂、聚酯纤维和医药等产品的关键成分。近年来，随着国内外市场的发展，开发生产戊二醇的需求不断增加，工业上通常采用不可再生的化石原料制备戊二醇，对环境污染严重，因此以可再生的生物质基为原料催化转化制备戊二醇是一种绿色生产工艺，更具有良好的应用前景和研究价值。本文系统总结了近年来以国内外生物质基平台化合物（木糖、糠醛、糠醇、四氢糠醇、γ-戊内酯和乙酰丙酸）为原料催化加氢制备戊二醇的研究现状，对制备戊二醇的催化剂进行了分析总结，从不同催化体系（贵金属和非贵金属）、反应机理、反应路径、反应条件及催化剂的稳定性等方面进行详细阐述，并且在此基础上从绿色环保和经济等多角度出发对完善生物质基制备戊二醇的生产工艺进行了展望，为进一步开发新型、高效、绿色和稳定的催化剂体系提供参考。
Keyword ：
乙酰丙酸 乙酰丙酸 催化剂 催化剂 四氢糠醇 四氢糠醇 戊二醇 戊二醇 糠醇 糠醇 糠醛 糠醛
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| GB/T 7714 | 邓佳慧 , 刘毅 , 张良清 et al. 生物质基平台化合物催化转化制备戊二醇 [J]. | 化学进展 , 2024 , 36 (07) : 1061-1087 . |
| MLA | 邓佳慧 et al. "生物质基平台化合物催化转化制备戊二醇" . | 化学进展 36 . 07 (2024) : 1061-1087 . |
| APA | 邓佳慧 , 刘毅 , 张良清 , 邱佳容 , 陈剑锋 , 曾宪海 . 生物质基平台化合物催化转化制备戊二醇 . | 化学进展 , 2024 , 36 (07) , 1061-1087 . |
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Global climate change intensifies the water cycle and makes freshest waters become fresher and vice-versa. But how this change impacts phytoplankton in coastal, particularly harmful algal blooms (HABs), remains poorly understood. Here, we monitored a coastal bay for a decade and found a significant correlation between salinity decline and the increase of Karenia mikimotoi blooms. To examine the physiological linkage between salinity decreases and K. mikimotoi blooms, we compare chemical, physiological and multi-omic profiles of this species in laboratory cultures under high (33) and low (25) salinities. Under low salinity, photosynthetic efficiency and capacity as well as growth rate and cellular protein content were significantly higher than that under high salinity. More strikingly, the omics data show that low salinity activated the glyoxylate shunt to bypass the decarboxylation reaction in the tricarboxylic acid cycle, hence redirecting carbon from CO2 release to biosynthesis. Furthermore, the enhanced glyoxylate cycle could promote hydrogen peroxide metabolism, consistent with the detected decrease in reactive oxygen species. These findings suggest that salinity declines can reprogram metabolism to enhance cell proliferation, thus promoting bloom formation in HAB species like K. mikimotoi, which has important ecological implications for future climate-driven salinity declines in the coastal ocean with respect to HAB outbreaks.
Keyword ：
global climate change global climate change harmful algal bloom harmful algal bloom Karenia mikimotoi Karenia mikimotoi ROS ROS salinity salinity
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| GB/T 7714 | Shi, Xinguo , Zou, Yazhen , Zhang, Yingjiao et al. Salinity decline promotes growth and harmful blooms of a toxic alga by diverting carbon flow [J]. | GLOBAL CHANGE BIOLOGY , 2024 , 30 (6) . |
| MLA | Shi, Xinguo et al. "Salinity decline promotes growth and harmful blooms of a toxic alga by diverting carbon flow" . | GLOBAL CHANGE BIOLOGY 30 . 6 (2024) . |
| APA | Shi, Xinguo , Zou, Yazhen , Zhang, Yingjiao , Ding, Guangmao , Xiao, Yuchun , Lin, Senjie et al. Salinity decline promotes growth and harmful blooms of a toxic alga by diverting carbon flow . | GLOBAL CHANGE BIOLOGY , 2024 , 30 (6) . |
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Dinoflagellate blooms have negative adverse effects on marine ecosystems. However, our knowledge about the spatiotemporal distribution of dinoflagellate communities and their correlations with micro-eukaryotic and bacterial communities is still rare. Here, the sediment micro-eukaryotic and bacterial communities were explored in the Taiwan Strait (TWS) by 16S and 18S rRNA gene high-throughput sequencing. We found that the dinoflagellates were the most abundant algal group in TWS, and their relative abundance was higher in spring and autumn than in summer. Moreover, the species richness and community composition of dinoflagellates showed strong seasonal patterns. NO3-N and NH4-N had the strongest correlations with the spatiotemporal dynamics of community composition of dinoflagellates. The dinoflagellates had a significantly wider niche breadth than other algal groups for NH4-N, NO3-N and NO2-N, and therefore potentially contributed to a wider distribution range and high abundance in TWS. In addition, the dinoflagellates had stronger impacts on microeukaryotes than on bacteria for both community composition and species richness. However, the dinoflagellates showed close coexistence with bacteria but loose coexistence with microeukaryotes in spring co-occurrence networks. This close coexistence suggests the potentially strong synergy effects between dinoflagellates and bacteria in spring dinoflagellate blooms in TWS. Overall, this study revealed the distribution mechanisms of dinoflagellates in TWS based on niche breadth and also unveiled the different effects of dinoflagellates on micro-eukaryotic and bacterial communities. © 2024 Elsevier Ltd
Keyword ：
Abiotic Abiotic Anthropogenic Anthropogenic
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| GB/T 7714 | Wang, Hongwei , Liu, Lemian , Luo, Zhaohe et al. Spatiotemporal dynamics of dinoflagellate communities in the Taiwan Strait and their correlations with micro-eukaryotic and bacterial communities [J]. | Marine Pollution Bulletin , 2024 , 208 . |
| MLA | Wang, Hongwei et al. "Spatiotemporal dynamics of dinoflagellate communities in the Taiwan Strait and their correlations with micro-eukaryotic and bacterial communities" . | Marine Pollution Bulletin 208 (2024) . |
| APA | Wang, Hongwei , Liu, Lemian , Luo, Zhaohe , Chen, Jianfeng . Spatiotemporal dynamics of dinoflagellate communities in the Taiwan Strait and their correlations with micro-eukaryotic and bacterial communities . | Marine Pollution Bulletin , 2024 , 208 . |
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Chlorella meal is considered a promising alternative to fish meal in aquafeeds, but the effects of incorporating Chlorella meal as a feed ingredient on the growth and quality of eel have not been explored. The effects of partially substituting fish meal (0 %, 5 %, 10 %, 15 %, and 20 %) with Chlorella meal on the growth performance, whole-body composition, pigmentation, and physiological health of marbled eel (Anguilla marmorata) were evaluated in this study. The results revealed that dietary inclusion of Chlorella meal significantly increased the final body weight (FBW), weight gain rate (WGR), and specific growth rate (SGR), but it significantly decreased the feed conversion rate (FCR), with these parameters exhibiting linear trends (P < 0.05). The whole-body protein and fat contents were significantly higher in all groups fed with diets containing Chlorella meal compared to the control group (P < 0.05). In addition, the dorsal and abdominal skin of marbled eel exhibited an orange-yellow color, and the color intensity increased with increasing levels of dietary Chlorella meal. The color change corresponded to increased values of a* (redness), b* (yellowness), and lutein content in the fish skin (P < 0.05). The physiological health of marbled eel also improved in the groups where Chlorella meal was included. The optimal replacement rate was approximately 10 % (9 % Chlorella meal supplementation), because the whole-body protein content and most physiological health parameters were highest under this condition, and the growth performance did not show a significant increase when the fish meal replacement rate exceeded 10 % (P > 0.05). These findings indicate the potential of Chlorella meal as a promising alternative to fish meal in feed formulations, offering benefits in enhancing growth performance and quality of marbled eel.
Keyword ：
Chlorella meal Chlorella meal Growth performance Growth performance Marbled eel Marbled eel Physiological health Physiological health Pigmentation Pigmentation Whole-body composition Whole-body composition
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| GB/T 7714 | Huang, Kaiyun , Liu, Xinyu , Ma, Ruijuan et al. Effects of substituting fish meal with Chlorella meal on growth performance, whole-body composition, pigmentation, and physiological health of marbled eel ( Anguilla marmorata ) [J]. | ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS , 2024 , 80 . |
| MLA | Huang, Kaiyun et al. "Effects of substituting fish meal with Chlorella meal on growth performance, whole-body composition, pigmentation, and physiological health of marbled eel ( Anguilla marmorata )" . | ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 80 (2024) . |
| APA | Huang, Kaiyun , Liu, Xinyu , Ma, Ruijuan , Wang, Baobei , Ho, Shih-Hsin , Chen, Jianfeng et al. Effects of substituting fish meal with Chlorella meal on growth performance, whole-body composition, pigmentation, and physiological health of marbled eel ( Anguilla marmorata ) . | ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS , 2024 , 80 . |
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BackgroundChlorella sorokiniana FZU60 is a promising lutein producing microalga. A mixotrophy/photoautotrophy two-stage strategy can achieve high biomass concentration at stage 1 and high lutein content at stage 2, leading to excellent lutein production efficiency in C. sorokiniana FZU60. However, the underlying molecular mechanisms are still unclear, restraining the further improvement of lutein production.ResultsIn this study, physiological and biochemical analysis revealed that photochemical parameters (Fv/Fm and NPQ) and photosynthetic pigments contents increased during the shift from mixotrophy to photoautotrophy, indicating that photosynthesis and photoprotection enhanced. Furthermore, transcriptomic analysis revealed that the glyoxylate cycle and TCA cycle were suppressed after the shift to photoautotrophy, leading to a decreased cell growth rate. However, the gene expression levels of photosynthesis, CO2 fixation, autophagy, and lutein biosynthesis were upregulated at the photoautotrophy stage, demonstrating that microalgal cells could obtain more precursor to synthesize lutein for enhancing photosynthesis and reducing reactive oxygen species.ConclusionsThe findings help to elucidate the molecular mechanisms for high lutein production efficiency of C. sorokiniana FZU60 under the mixotrophy/photoautotrophy strategy, identify key functional genes responsible for lutein biosynthesis, and shed light on further improvement of lutein production by genetic or metabolic engineering in future studies.
Keyword ：
Chlorella sorokiniana FZU60 Chlorella sorokiniana FZU60 Lutein Lutein Mixotrophy Mixotrophy Molecular mechanisms Molecular mechanisms Photoautotrophy Photoautotrophy
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| GB/T 7714 | Ma, Ruijuan , Zhang, Zhen , Fang, Hong et al. Unveiling the underlying molecular mechanisms of high lutein production efficiency in Chlorella sorokiniana FZU60 under a mixotrophy/photoautotrophy two-stage strategy by transcriptomic, physiological, and biochemical analyses [J]. | BIOTECHNOLOGY FOR BIOFUELS AND BIOPRODUCTS , 2023 , 16 (1) . |
| MLA | Ma, Ruijuan et al. "Unveiling the underlying molecular mechanisms of high lutein production efficiency in Chlorella sorokiniana FZU60 under a mixotrophy/photoautotrophy two-stage strategy by transcriptomic, physiological, and biochemical analyses" . | BIOTECHNOLOGY FOR BIOFUELS AND BIOPRODUCTS 16 . 1 (2023) . |
| APA | Ma, Ruijuan , Zhang, Zhen , Fang, Hong , Liu, Xinyu , Ho, Shih-Hsin , Xie, Youping et al. Unveiling the underlying molecular mechanisms of high lutein production efficiency in Chlorella sorokiniana FZU60 under a mixotrophy/photoautotrophy two-stage strategy by transcriptomic, physiological, and biochemical analyses . | BIOTECHNOLOGY FOR BIOFUELS AND BIOPRODUCTS , 2023 , 16 (1) . |
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Microalgae can accumulate various compounds simultaneously, thus co-production of high-value metabolites to improve the economic value of microalgae production is feasible. In this study, a microalga strain Nitzschia sp. FZU62 capable of co-producing fucoxanthin and eicosapentaenoic acid (EPA) was isolated and identified. Further, heterotrophic conditions were optimized to improve the co-production efficiency in flasks. Results showed that the optimal conditions for flask culture were cultivation for 84 h with an inoculum size of 0.47 g/L and a temperature of 19 degrees C. Besides, three fed-batch culture strategies with distinct nutrient compositions were explored to improve the heterotrophic performance of strain FZU62. Results indicated that the highest biomass, fucoxanthin, and EPA productivities were obtained by Fed-batch III, in which concentrated medium was pulse -fed to supply 60 % initial nutrients once glucose concentration dropped to about 2 g/L. Moreover, the hetero-trophic performance was verified in a 5 L fermenter, resulting in excellent fucoxanthin and EPA productions of 63.03 and 398.60 mg/L, and productivities of 3.41 and 22.52 mg/L/d, respectively. These results, especially the EPA production and productivity, are superior to many literature reports. The present study provides new in-sights into the highly efficient co-production of fucoxanthin and EPA by marine microalgae and has guiding significance for commercial production in heterotrophic mode.
Keyword ：
Co -production Co -production Eicosapentaenoic acid Eicosapentaenoic acid Fucoxanthin Fucoxanthin Heterotrophic cultivation Heterotrophic cultivation Microalgae Microalgae
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| GB/T 7714 | Ma, Ruijuan , You, Yuanwei , Liu, Xinyu et al. Highly efficient co-production of fucoxanthin and eicosapentaenoic acid by heterotrophic cultivation of a newly isolated microalga Nitzschia sp. FZU62 [J]. | ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS , 2023 , 71 . |
| MLA | Ma, Ruijuan et al. "Highly efficient co-production of fucoxanthin and eicosapentaenoic acid by heterotrophic cultivation of a newly isolated microalga Nitzschia sp. FZU62" . | ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 71 (2023) . |
| APA | Ma, Ruijuan , You, Yuanwei , Liu, Xinyu , Ho, Shih-Hsin , Xie, Youping , Chen, Jianfeng . Highly efficient co-production of fucoxanthin and eicosapentaenoic acid by heterotrophic cultivation of a newly isolated microalga Nitzschia sp. FZU62 . | ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS , 2023 , 71 . |
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